##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921297_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1397258 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.285757533683828 33.0 30.0 34.0 19.0 34.0 2 30.03802733639743 33.0 31.0 34.0 26.0 34.0 3 30.27886904208099 33.0 31.0 34.0 26.0 34.0 4 32.5047292625986 37.0 35.0 37.0 19.0 37.0 5 32.1457955510006 37.0 33.0 37.0 19.0 37.0 6 32.28170745846508 37.0 35.0 37.0 17.0 37.0 7 32.48059127233481 37.0 35.0 37.0 17.0 37.0 8 32.73302568315945 37.0 35.0 37.0 17.0 37.0 9 34.48365298319995 39.0 37.0 39.0 17.0 39.0 10-11 34.66862669600031 39.0 37.0 39.0 24.0 39.0 12-13 34.680362538629225 39.0 37.0 39.0 25.0 39.0 14-15 36.09367561323678 40.5 38.0 41.0 26.0 41.0 16-17 36.11294299263271 41.0 38.0 41.0 26.0 41.0 18-19 36.1356753727658 41.0 38.0 41.0 26.0 41.0 20-21 36.13036425627908 41.0 38.0 41.0 26.0 41.0 22-23 36.07998701742986 41.0 38.0 41.0 25.5 41.0 24-25 36.03167382115544 41.0 38.0 41.0 25.0 41.0 26-27 35.943169049667276 41.0 38.0 41.0 25.0 41.0 28-29 35.86772163766463 40.0 38.0 41.0 24.5 41.0 30-31 35.777117039229694 40.0 37.5 41.0 24.0 41.0 32-33 35.65992178967664 40.0 37.0 41.0 23.5 41.0 34-35 35.57518654393104 40.0 37.0 41.0 23.0 41.0 36-37 35.49871033123446 40.0 37.0 41.0 22.0 41.0 38-39 35.40218091433364 40.0 36.5 41.0 20.5 41.0 40-41 35.277635912623154 40.0 36.0 41.0 18.5 41.0 42-43 35.14417451895069 40.0 36.0 41.0 17.0 41.0 44-45 35.000259078853006 40.0 35.5 41.0 15.0 41.0 46-47 34.79832965708552 40.0 35.0 41.0 10.5 41.0 48-49 34.59199839972288 40.0 35.0 41.0 7.5 41.0 50-51 34.41050614847079 40.0 35.0 41.0 4.0 41.0 52-53 34.22555032785642 39.5 35.0 41.0 2.0 41.0 54-55 34.02192580038905 39.0 35.0 41.0 2.0 41.0 56-57 33.774622510660166 39.0 35.0 41.0 2.0 41.0 58-59 33.706799674791625 39.0 35.0 41.0 2.0 41.0 60-61 33.46579658159051 38.0 34.5 41.0 2.0 41.0 62-63 33.178459167884526 37.0 34.0 40.0 2.0 41.0 64-65 32.86719775445909 37.0 34.0 40.0 2.0 41.0 66-67 32.5458097216119 36.0 34.0 39.0 2.0 41.0 68-69 32.208304765476385 36.0 34.0 39.0 2.0 41.0 70-71 31.876344239932784 35.0 33.5 38.5 2.0 40.5 72-73 31.53682498149948 35.0 33.0 37.0 2.0 39.5 74-75 31.202491236407305 35.0 33.0 37.0 2.0 39.0 76-77 30.90591071942333 35.0 33.0 36.0 2.0 39.0 78-79 30.64850299658331 35.0 33.0 36.0 2.0 37.5 80-81 30.422687148686926 35.0 33.0 35.5 2.0 37.0 82-83 30.224936625877255 35.0 33.0 35.0 2.0 37.0 84-85 30.06000108784491 35.0 32.0 35.0 2.0 36.0 86-87 29.9161142752448 35.0 32.0 35.0 2.0 36.0 88-89 29.796076673026743 35.0 32.0 35.0 2.0 36.0 90-91 29.695730852856094 35.0 32.0 35.0 2.0 35.5 92-93 29.625620322087975 35.0 32.0 35.0 2.0 35.0 94-95 29.556164287483057 35.0 32.0 35.0 2.0 35.0 96-97 29.498042594853636 35.0 32.0 35.0 2.0 35.0 98-99 29.440239025290964 35.0 32.0 35.0 2.0 35.0 100-101 29.38915254018943 35.0 32.0 35.0 2.0 35.0 102-103 28.717046529703175 34.0 30.0 35.0 2.0 35.0 104-105 29.261336846881534 35.0 31.5 35.0 2.0 35.0 106-107 29.32206900944564 35.0 32.0 35.0 2.0 35.0 108-109 29.391372602626 35.0 32.0 35.0 2.0 35.0 110-111 29.500220431731293 35.0 32.0 35.0 2.0 35.0 112-113 29.465914312174274 35.0 32.0 35.0 2.0 35.0 114-115 29.404950266879844 35.0 32.0 35.0 2.0 35.0 116-117 29.347828389603066 35.0 32.0 35.0 2.0 35.0 118-119 29.273144973941818 35.0 32.0 35.0 2.0 35.0 120-121 29.18016071477136 35.0 32.0 35.0 2.0 35.0 122-123 29.07119587076975 35.0 32.0 35.0 2.0 35.0 124-125 28.54162652852945 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 91473.0 3 6166.0 4 4457.0 5 4115.0 6 4200.0 7 4469.0 8 4735.0 9 4828.0 10 4781.0 11 4924.0 12 5192.0 13 6028.0 14 7200.0 15 6301.0 16 4266.0 17 3735.0 18 3411.0 19 3155.0 20 2965.0 21 3110.0 22 3514.0 23 4434.0 24 5777.0 25 7516.0 26 8921.0 27 10444.0 28 12335.0 29 14821.0 30 17747.0 31 22080.0 32 28084.0 33 37761.0 34 57602.0 35 98234.0 36 229660.0 37 489314.0 38 169261.0 39 242.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.16969360547657 17.886023563948466 11.64567760893497 27.29860522164 2 16.00828193504707 19.3297873406343 37.544104238444156 27.117826485874474 3 19.262942133807787 22.476665011043057 28.33964808217237 29.920744772976786 4 13.53091538220917 15.302969242453418 31.436418727537056 39.72969664780036 5 14.426149124737536 37.09529998501742 32.372058413437586 16.106492476807464 6 35.20163992645248 33.47586507122769 15.942599710163192 15.37989529215665 7 30.626127902839826 31.11726896648487 18.48117793680666 19.77542519386865 8 28.16206431812212 34.90515328000092 17.89537121667052 19.037411185206444 9 27.482208717173894 15.888869882255097 17.74096693823886 38.887954462332154 10-11 27.022609019273848 25.096190319417705 25.602334619210865 22.27886604209758 12-13 26.493429490277965 22.296445243604037 26.34788362345834 24.862241642659654 14-15 23.86098344042403 23.583260929620728 23.552056957269166 29.003698672686074 16-17 24.311580252179628 26.75740629146514 24.169766786091042 24.76124667026419 18-19 24.421652980337203 25.857429336600685 25.815454268288317 23.90546341477379 20-21 24.99860440949345 24.960601406468953 25.77265615942081 24.268138024616785 22-23 24.89694100874713 24.37767398719492 25.681048167196035 25.044336836861913 24-25 24.54976103196403 24.83217129549446 25.63735544902946 24.98071222351205 26-27 24.31283270519832 25.00286274975703 25.771904687609588 24.912399857435062 28-29 24.41739464007363 25.326496609788602 25.608191185879775 24.647917564257995 30-31 24.827197267791632 25.065485400691927 25.50309248542503 24.604224846091416 32-33 24.5340159083004 25.15455270250734 25.5635322896702 24.747899099522066 34-35 24.589839528562372 25.213883191221665 25.747463961558996 24.44881331865697 36-37 24.65947591640198 25.08813690814438 25.801498363222823 24.45088881223081 38-39 24.503456054644168 25.41474087104887 25.672066289833374 24.40973678447359 40-41 24.510934988384392 25.40450654066751 25.629518671569603 24.455039799378497 42-43 24.388946064363203 25.322095132037177 25.51440034696527 24.774558456634352 44-45 24.29426061614963 25.389942301278644 25.668201577661392 24.64759550491033 46-47 24.315087120631983 25.36875795307667 25.39416485717026 24.92199006912109 48-49 24.350298942643377 25.554264137331835 25.282660754134167 24.812776165890625 50-51 24.271036558745774 25.70434379334382 25.37630845556082 24.64831119234959 52-53 24.472788847872046 25.575520054277735 25.303701964848297 24.64798913300192 54-55 24.156311862233032 25.347788311106466 25.534546948380328 24.961352878280174 56-57 24.25683016307654 25.520054277735394 25.31830198860912 24.90481357057895 58-59 24.260659090876562 25.5209131026625 25.47850862188658 24.73991918457436 60-61 24.533980123928437 25.363497650398138 25.56288817097487 24.53963405469856 62-63 24.347185702282616 25.524205264883076 25.52796262393917 24.600646408895134 64-65 24.26037281590086 25.242474904419943 25.49954983260071 24.997602447078492 66-67 24.265561549835464 25.43113011340783 25.390049654394538 24.91325868236217 68-69 24.051606789869872 25.402323693977774 25.57788182282728 24.96818769332507 70-71 24.093259798834573 25.29160684712487 25.59895881791337 25.016174536127185 72-73 24.186227597193934 25.50788759126804 25.261118562212566 25.044766249325463 74-75 24.033356760168843 25.574661229350625 25.357736366512125 25.0342456439684 76-77 24.01832732394447 25.311109329844594 25.40418448131984 25.26637886489109 78-79 23.95642036044882 25.2996583308165 25.53197047359901 25.21195083513567 80-81 24.131227017487106 25.552653840593504 25.235353814399346 25.080765327520044 82-83 24.20225899583327 25.4524218147257 25.37884914597018 24.966470043470856 84-85 24.107931391339324 25.560454833681394 25.152190933957797 25.179422841021488 86-87 23.80490932955832 25.661688821964162 25.36178000054392 25.171621847933594 88-89 23.7714509417731 25.402609968953477 25.413452633658206 25.412486455615213 90-91 23.98129762237472 25.30965167072164 25.476407142430286 25.232643564473356 92-93 23.975229960225512 25.47921724956656 25.428224318797067 25.11732847141086 94-95 23.98786479075245 25.330925281077597 25.36917881577219 25.312031112397758 96-97 23.74424092996792 25.244041431308943 25.45441858218182 25.55729905654132 98-99 23.663855803243138 25.346294437496308 25.37395576692199 25.615893992338563 100-101 23.664276747744513 25.409444783998374 25.441292875045267 25.484985593211846 102-103 23.51917111943535 25.304847064751108 25.517513587326036 25.658468228487507 104-105 23.543378204582265 25.44199098662594 25.380620744787823 25.634010064003977 106-107 23.643698792490426 25.245624280733093 25.46763084216493 25.643046084611555 108-109 23.445993795676724 25.244954111697144 25.54651041705806 25.76254167556807 110-111 23.394965043873633 25.57363509718902 25.477768318731453 25.553631540205895 112-113 23.4558195192543 25.572483299290177 25.452711977704713 25.518985203750805 114-115 23.328746951606814 25.61594987305444 25.382992694591707 25.672310480747036 116-117 23.202315121469084 25.480113236836537 25.475389667621755 25.842181974072613 118-119 23.251936745909028 25.326864452298743 25.55864057595848 25.862558225833755 120-121 23.159885103415156 25.39802105053725 25.610258388492017 25.831835457555574 122-123 23.091072011359667 25.49154609425547 25.401725134981934 26.015656759402933 124-125 22.8970210183388 25.441186153432156 25.392984535403677 26.26880829282537 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 48.0 1 43.0 2 50.0 3 62.5 4 55.0 5 71.0 6 142.0 7 282.5 8 414.0 9 473.0 10 462.5 11 421.5 12 434.5 13 482.0 14 523.5 15 577.5 16 652.5 17 706.0 18 739.5 19 808.0 20 887.5 21 936.0 22 1003.0 23 1145.0 24 1314.0 25 1502.0 26 1831.0 27 2280.5 28 2858.5 29 3818.5 30 5051.5 31 6329.0 32 8040.0 33 10085.5 34 12169.5 35 14485.0 36 17375.5 37 20348.0 38 23090.5 39 26248.0 40 29671.5 41 32480.5 42 35618.0 43 39615.0 44 43889.5 45 48077.0 46 51552.0 47 55152.0 48 59283.0 49 62617.0 50 64241.0 51 65688.0 52 66042.5 53 65194.5 54 64599.0 55 63041.5 56 59097.0 57 53543.5 58 48805.5 59 43807.5 60 37513.5 61 31378.0 62 25638.0 63 19859.5 64 14706.0 65 11054.0 66 8394.0 67 6366.0 68 4913.5 69 3710.0 70 2901.5 71 2301.5 72 1794.5 73 1413.5 74 1098.5 75 814.0 76 608.0 77 431.5 78 294.0 79 239.0 80 185.5 81 149.5 82 134.0 83 118.0 84 102.5 85 99.0 86 99.0 87 80.0 88 77.5 89 89.0 90 80.5 91 67.5 92 60.0 93 52.0 94 46.5 95 40.0 96 38.0 97 36.0 98 24.5 99 9.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3368740776578127 2 0.0 3 0.0 4 0.01681865482251667 5 0.16496595474851458 6 0.04365693379461774 7 0.021971604385160076 8 0.007800993087890711 9 0.006083343233676243 10-11 0.0020039248299168803 12-13 0.056610876445151864 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 1.7892185981400715E-4 92-93 3.9362809159081575E-4 94-95 1.0735311588840429E-4 96-97 3.9362809159081575E-4 98-99 3.578437196280143E-5 100-101 0.0 102-103 0.0 104-105 7.156874392560286E-5 106-107 1.4313748785120572E-4 108-109 4.6519683551641855E-4 110-111 4.6519683551641855E-4 112-113 8.588249271072343E-4 114-115 3.9362809159081575E-4 116-117 6.799030672932271E-4 118-119 3.220593476652129E-4 120-121 1.0735311588840429E-4 122-123 0.0024333372934704973 124-125 1.4313748785120572E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1397258.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.18721382480214 #Duplication Level Percentage of deduplicated Percentage of total 1 83.96073315507067 57.25048464531959 2 8.864776145828882 12.089287731292785 3 2.83906345224433 5.807634800410951 4 1.3668412282122497 3.728043803706554 5 0.8091095158569342 2.7585461782709455 6 0.4783922286952894 1.957213991410161 7 0.3521546122460641 1.6808709291228863 8 0.2362008141123655 1.2884700333977768 9 0.1939524941464966 1.1902572171198764 >10 0.8631812622338118 10.021788273188672 >50 0.028449823052061774 1.2705493361789022 >100 0.006724982811244418 0.6649703472403423 >500 2.1014274480808827E-4 0.11284125515743455 >1k 2.1014274480808827E-4 0.1790414581831295 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.156874392560286E-5 2 0.0 0.0 0.0 0.0 7.156874392560286E-5 3 0.0 0.0 0.0 0.0 7.156874392560286E-5 4 0.0 0.0 0.0 0.0 7.156874392560286E-5 5 0.0 0.0 0.0 0.0 7.156874392560286E-5 6 0.0 0.0 0.0 0.0 1.4313748785120572E-4 7 7.156874392560286E-5 0.0 0.0 0.0 1.4313748785120572E-4 8 7.156874392560286E-5 0.0 0.0 0.0 2.1470623177680858E-4 9 7.156874392560286E-5 0.0 0.0 0.0 2.1470623177680858E-4 10-11 7.156874392560286E-5 0.0 0.0 0.0 2.1470623177680858E-4 12-13 7.156874392560286E-5 0.0 0.0 0.0 2.1470623177680858E-4 14-15 7.156874392560286E-5 7.156874392560286E-5 0.0 0.0 3.578437196280143E-4 16-17 7.156874392560286E-5 1.4313748785120572E-4 0.0 0.0 5.009812074792201E-4 18-19 7.156874392560286E-5 1.4313748785120572E-4 0.0 0.0 7.156874392560286E-4 20-21 7.156874392560286E-5 1.4313748785120572E-4 0.0 0.0 7.872561831816315E-4 22-23 7.156874392560286E-5 1.4313748785120572E-4 0.0 7.156874392560286E-5 7.872561831816315E-4 24-25 7.156874392560286E-5 1.4313748785120572E-4 0.0 5.367655794420215E-4 8.588249271072343E-4 26-27 7.156874392560286E-5 1.4313748785120572E-4 0.0 8.23040555144433E-4 9.661780429956387E-4 28-29 7.156874392560286E-5 1.4313748785120572E-4 0.0 0.0028627497570241143 0.0010735311588840428 30-31 7.156874392560286E-5 1.4313748785120572E-4 0.0 0.011307861540245252 0.0011093155308468444 32-33 7.156874392560286E-5 1.4313748785120572E-4 0.0 0.02716033831976629 0.001180884274772447 34-35 7.156874392560286E-5 1.4313748785120572E-4 0.0 0.04745007722267469 0.0012166686467352486 36-37 7.156874392560286E-5 1.4313748785120572E-4 0.0 0.0749324748901062 0.0012166686467352486 38-39 7.156874392560286E-5 1.4313748785120572E-4 0.0 0.11837470245294712 0.0012524530186980502 40-41 7.156874392560286E-5 1.4313748785120572E-4 0.0 0.17233753537285168 0.0012882373906608516 42-43 7.156874392560286E-5 1.4313748785120572E-4 0.0 0.2204317312908568 0.0012882373906608516 44-45 7.156874392560286E-5 2.1470623177680858E-4 0.0 0.27657741090049226 0.0012882373906608516 46-47 7.156874392560286E-5 2.1470623177680858E-4 0.0 0.3350490746877098 0.0012882373906608516 48-49 7.156874392560286E-5 2.1470623177680858E-4 0.0 0.39974721919645473 0.0013955905065492556 50-51 7.156874392560286E-5 2.1470623177680858E-4 0.0 0.4686679195968103 0.0014313748785120572 52-53 7.156874392560286E-5 2.1470623177680858E-4 0.0 0.5407376447298924 0.001646081110288866 54-55 7.156874392560286E-5 2.1470623177680858E-4 0.0 0.6189622818405763 0.0017892185981400715 56-57 7.156874392560286E-5 2.1470623177680858E-4 0.0 0.7034491840447505 0.0017892185981400715 58-59 7.156874392560286E-5 2.1470623177680858E-4 0.0 0.7982062010022487 0.0017892185981400715 60-61 7.156874392560286E-5 2.1470623177680858E-4 0.0 0.8966490082719154 0.0017892185981400715 62-63 7.156874392560286E-5 2.1470623177680858E-4 0.0 1.0056839896425713 0.0019323560859912771 64-65 7.156874392560286E-5 2.1470623177680858E-4 0.0 1.1094228839627327 0.00200392482991688 66-67 7.156874392560286E-5 2.1470623177680858E-4 0.0 1.2204617901633057 0.002075493573842483 68-69 7.156874392560286E-5 2.1470623177680858E-4 0.0 1.3501085697845352 0.002075493573842483 70-71 7.156874392560286E-5 2.1470623177680858E-4 0.0 1.4885583049086137 0.0021470623177680856 72-73 7.156874392560286E-5 2.1470623177680858E-4 0.0 1.6228212685130448 0.0025049060373961 74-75 7.156874392560286E-5 2.1470623177680858E-4 0.0 1.7628455160034868 0.002576474781321703 76-77 7.156874392560286E-5 2.1470623177680858E-4 0.0 1.919795771432334 0.002648043525247306 78-79 7.156874392560286E-5 2.1470623177680858E-4 0.0 2.0861931010593606 0.002648043525247306 80-81 7.156874392560286E-5 2.1470623177680858E-4 0.0 2.2587095582920265 0.0027196122691729085 82-83 7.156874392560286E-5 2.8627497570241143E-4 0.0 2.443356917620082 0.0027911810130985116 84-85 7.156874392560286E-5 2.8627497570241143E-4 0.0 2.6350895825967715 0.0027911810130985116 86-87 7.156874392560286E-5 2.8627497570241143E-4 0.0 2.8397046214800703 0.002826965385061313 88-89 7.156874392560286E-5 2.8627497570241143E-4 0.0 3.0680089146027436 0.002934318500949717 90-91 7.156874392560286E-5 2.8627497570241143E-4 0.0 3.3118436251572723 0.002934318500949717 92-93 7.156874392560286E-5 2.8627497570241143E-4 0.0 3.5537817639977725 0.00300588724487532 94-95 7.156874392560286E-5 2.8627497570241143E-4 0.0 3.8048807020607507 0.003077455988800923 96-97 7.156874392560286E-5 2.8627497570241143E-4 0.0 4.089294890421096 0.0033637309645033345 98-99 7.156874392560286E-5 2.8627497570241143E-4 0.0 4.399545395338585 0.00350686845235454 100-101 7.156874392560286E-5 2.8627497570241143E-4 0.0 4.729978286043092 0.003578437196280143 102-103 7.156874392560286E-5 2.8627497570241143E-4 0.0 5.071611685171958 0.0037931434280569516 104-105 7.156874392560286E-5 2.8627497570241143E-4 0.0 5.431960310837368 0.0037931434280569516 106-107 7.156874392560286E-5 3.578437196280143E-4 0.0 5.813457500332795 0.0038647121719825542 108-109 7.156874392560286E-5 3.578437196280143E-4 0.0 6.209161085497453 0.003936280915908157 110-111 7.156874392560286E-5 3.578437196280143E-4 0.0 6.639897570813694 0.003936280915908157 112-113 7.156874392560286E-5 3.578437196280143E-4 0.0 7.071814940404707 0.003936280915908157 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1440 0.0 56.030388 1 GTATCAA 2500 0.0 48.529785 1 GTACATG 6315 0.0 37.66709 1 TCAACGC 3275 0.0 35.669464 4 TACATGG 6645 0.0 35.24921 2 ACATGGG 6530 0.0 35.139805 3 CAACGCA 3360 0.0 34.76711 5 TATCAAC 3590 0.0 34.03386 2 ATCAACG 3540 0.0 32.83093 3 AACGCAG 3555 0.0 32.513092 6 CATGGGG 6065 0.0 32.429104 4 ATGGGGG 3625 0.0 28.608366 5 ACGCAGA 4475 0.0 25.823282 7 GTGGTAT 850 0.0 25.312551 1 CGCAGAG 4890 0.0 23.382244 8 TGGTATC 985 0.0 21.177935 2 CTACACT 685 0.0 20.882019 4 TACCGTA 200 4.867965E-5 20.848307 7 ATGGGGA 2405 0.0 20.816898 5 TGGGGGG 2580 0.0 20.783512 6 >>END_MODULE