FastQCFastQC Report
Mon 15 Aug 2016
SRR2921296_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921296_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1939763
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16750.056.8415871
GTATCAA33200.053.2328071
TCAACGC39700.043.465644
CAACGCA40150.042.6809775
TATCAAC43100.040.3129162
ATCAACG43000.039.9915283
AACGCAG42900.039.942966
GTACATG99200.037.4312171
TACATGG105050.035.0617182
ACATGGG106950.033.7155653
ACGCAGA53200.032.2054867
CATGGGG96600.031.8457894
ATGGGGG57550.030.50035
CGCAGAG57800.029.6416578
GCAGAGT66950.025.2344829
TGGGGGG40300.024.2127326
AGTACAT70500.022.6195662
GAGTACA71400.022.5856231
AGAGTAC59300.022.22008310-11
CAGAGTA64250.020.73965510-11
Produced by FastQC (version 0.11.5)