##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921296_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1939763
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.608973364271822	31.0	31.0	34.0	27.0	34.0
2	30.837416735962073	33.0	31.0	34.0	27.0	34.0
3	30.912034614537962	33.0	31.0	34.0	27.0	34.0
4	34.35377260005475	37.0	35.0	37.0	32.0	37.0
5	34.377283204185254	37.0	35.0	37.0	32.0	37.0
6	34.441170390403364	37.0	35.0	37.0	32.0	37.0
7	34.386329670171044	37.0	35.0	37.0	31.0	37.0
8	34.32774570914076	37.0	35.0	37.0	31.0	37.0
9	36.036950905858085	39.0	37.0	39.0	32.0	39.0
10-11	36.03297774006412	39.0	37.0	39.0	32.0	39.0
12-13	35.97382695721075	39.0	37.0	39.0	31.5	39.0
14-15	37.3274616538206	41.0	38.0	41.0	31.0	41.0
16-17	37.32148489274205	41.0	38.0	41.0	31.0	41.0
18-19	37.32225766756042	41.0	38.0	41.0	31.0	41.0
20-21	37.35433735977024	41.0	38.0	41.0	31.0	41.0
22-23	37.30896377547154	41.0	38.0	41.0	31.0	41.0
24-25	37.29808358031367	41.0	38.0	41.0	31.0	41.0
26-27	37.27299907256712	41.0	38.0	41.0	31.0	41.0
28-29	37.21073888923544	41.0	38.0	41.0	30.0	41.0
30-31	37.133541056304296	40.5	38.0	41.0	30.0	41.0
32-33	37.0618807555356	40.0	38.0	41.0	30.0	41.0
34-35	36.958569423171795	40.0	38.0	41.0	30.0	41.0
36-37	36.85322897694203	40.0	38.0	41.0	30.0	41.0
38-39	36.755916573313336	40.0	38.0	41.0	30.0	41.0
40-41	36.652044863212666	40.0	37.5	41.0	30.0	41.0
42-43	36.468254111455884	40.0	37.0	41.0	29.5	41.0
44-45	36.228051571248656	40.0	36.5	41.0	28.5	41.0
46-47	36.01465694520413	40.0	36.0	41.0	28.0	41.0
48-49	35.813858703357056	40.0	35.5	41.0	27.5	41.0
50-51	35.64288163038474	40.0	35.0	41.0	27.0	41.0
52-53	35.45226452922341	39.0	35.0	41.0	27.0	41.0
54-55	35.247243348800865	39.0	35.0	41.0	27.0	41.0
56-57	34.99816369319345	39.0	35.0	40.5	26.0	41.0
58-59	34.73061090452803	38.0	35.0	40.0	26.0	41.0
60-61	34.44480253515507	38.0	34.0	40.0	26.0	41.0
62-63	34.12131198502085	37.0	34.0	40.0	25.5	41.0
64-65	33.77186027365199	37.0	34.0	39.5	24.5	41.0
66-67	33.41887823409355	36.0	34.0	39.0	23.0	41.0
68-69	33.061009257316485	35.5	33.0	39.0	23.0	40.0
70-71	33.00051990887546	35.0	34.0	39.0	24.0	40.0
72-73	32.70133542087358	35.0	33.5	37.5	23.0	40.0
74-75	32.378984442944834	35.0	33.0	37.0	23.0	39.0
76-77	32.0769421315903	35.0	33.0	36.5	22.0	39.0
78-79	31.790656384310868	35.0	33.0	36.0	21.5	38.0
80-81	31.51583775956135	35.0	33.0	36.0	20.0	37.0
82-83	31.298140030508883	35.0	33.0	35.0	20.0	37.0
84-85	31.092410773893512	35.0	33.0	35.0	19.5	36.0
86-87	30.925332630842014	35.0	33.0	35.0	18.5	36.0
88-89	30.789930780203562	35.0	32.5	35.0	18.0	36.0
90-91	30.675165729009162	35.0	32.0	35.0	17.5	35.5
92-93	30.568189000408815	35.0	32.0	35.0	17.0	35.0
94-95	30.451799266199014	35.0	32.0	35.0	14.0	35.0
96-97	30.366981172442202	35.0	32.0	35.0	10.5	35.0
98-99	30.292025108221985	35.0	32.0	35.0	8.5	35.0
100-101	30.20399631295163	35.0	32.0	35.0	7.0	35.0
102-103	29.37717623235416	34.0	30.0	35.0	7.0	35.0
104-105	29.976192710140364	34.5	31.5	35.0	6.0	35.0
106-107	30.056295794898656	35.0	32.0	35.0	2.0	35.0
108-109	29.996263461051683	35.0	32.0	35.0	2.0	35.0
110-111	29.919002476075686	35.0	31.5	35.0	2.0	35.0
112-113	29.83812094570316	35.0	31.0	35.0	2.0	35.0
114-115	29.720457602294715	35.0	31.0	35.0	2.0	35.0
116-117	29.594048087317883	34.0	31.0	35.0	2.0	35.0
118-119	29.591728989572438	34.5	31.5	35.0	2.0	35.0
120-121	29.75964512159475	35.0	32.0	35.0	2.0	35.0
122-123	29.636367432516238	35.0	32.0	35.0	2.0	35.0
124-125	29.08135297971969	34.5	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	58029.0
3	8930.0
4	5524.0
5	4539.0
6	4987.0
7	5582.0
8	6054.0
9	6129.0
10	6697.0
11	7552.0
12	7877.0
13	7974.0
14	8104.0
15	7004.0
16	5557.0
17	5182.0
18	4874.0
19	4687.0
20	4656.0
21	4754.0
22	5613.0
23	7475.0
24	10900.0
25	13794.0
26	14870.0
27	16138.0
28	19675.0
29	23313.0
30	28486.0
31	35705.0
32	46744.0
33	64034.0
34	100529.0
35	170208.0
36	369075.0
37	651360.0
38	187073.0
39	79.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.94319066549109	18.203065786013706	13.014792701098754	24.838950847396454
2	16.31865800421642	19.12929691749516	39.43277827588643	25.11926680240199
3	19.784395954988053	23.554605289615143	28.32761214575984	28.333386609636964
4	13.569735223421029	15.053760668865834	35.35979483604723	36.0167092716659
5	14.692002992349504	37.37224900018715	32.83742684752061	15.098321159942731
6	34.02875935633085	33.97203478763095	17.238647995688932	14.76055786034927
7	29.864312418118004	31.90037375053428	19.653689117306314	18.581624714041396
8	27.38648942812522	34.9178384693635	18.770549405449664	18.92512269706161
9	27.487244897959183	16.046062725817894	18.39230033161203	38.074392044610896
10-11	26.475258632334274	25.512031700956783	26.4433710416338	21.569338625075144
12-13	26.16878538810431	22.856516824534292	26.819004950629044	24.155692836732353
14-15	23.873423112000612	24.18718960394585	23.96075805077078	27.97862923328276
16-17	24.278060254551313	27.292360238440473	24.58885774125987	23.84072176574835
18-19	24.294489755936606	26.754740521534703	25.888996009609933	23.06177371291876
20-21	25.226017827774825	25.59757587630886	25.666479349580086	23.509926946336233
22-23	25.186056085299025	24.996745581272695	25.567744624666922	24.249453708761358
24-25	24.62676679356097	25.347739205391427	25.556047620422422	24.46944638062518
26-27	24.257935928970127	25.60281508755776	25.718658281138595	24.420590702333516
28-29	24.315339776193483	26.037285275587323	25.579835502649555	24.06753944556964
30-31	24.997312693444186	25.566351467733373	25.514590205727817	23.921745633094623
32-33	24.58785911525303	25.552013066423047	25.513448970627355	24.346678847696566
34-35	24.608753305393343	25.787558780449704	25.636834390835794	23.966853523321163
36-37	24.62505448796522	25.754537484497835	25.575123533980516	24.045284493556434
38-39	24.59879401047198	25.87729654597835	25.514551063799075	24.009358379750594
40-41	24.59420346641287	25.611427263381593	25.6047765515492	24.189592718656332
42-43	24.360798640164937	25.624250185729668	25.462210391890892	24.552740782214496
44-45	24.230187542098566	25.648669124677227	25.73347848020604	24.38766485301817
46-47	24.3132361038645	25.551143429277317	25.62213730225345	24.513483164604732
48-49	24.19389805516781	25.769097416900927	25.668382202252754	24.368622325678512
50-51	24.278781585930385	25.775222473525123	25.66270327566198	24.283292664882513
52-53	24.375366523558682	25.742475231062894	25.55656803772792	24.3255902076505
54-55	24.10517201334976	25.463167394500225	25.79526159345927	24.63639899869075
56-57	24.321619025008037	25.62835871566657	25.52058078923551	24.529441470089886
58-59	24.34969598904545	25.680425416847	25.73512599305906	24.234752601048488
60-61	24.63136979740483	25.646717166379418	25.688296168287465	24.033616867928284
62-63	24.463975232932363	25.738623401630946	25.607286797126832	24.190114568309856
64-65	24.265022979027464	25.49247861851051	25.667531145868427	24.574967256593595
66-67	24.33389742472731	25.734631302045806	25.52323235968495	24.408238913541933
68-69	24.179018064515464	25.72238213005482	25.64303828662667	24.455561518803048
70-71	24.140330110556697	25.57937931626474	25.831972071732324	24.44831850144624
72-73	24.210119034423524	25.930393036829063	25.464906523771052	24.39458140497636
74-75	24.1467539621554	25.91355189512993	25.536327738039294	24.40336640467537
76-77	24.152073028296066	25.545777629529308	25.55191264155316	24.750236700621468
78-79	24.046010006142676	25.61879787607262	25.71203353493579	24.623158582848916
80-81	24.231579126451216	25.927607163653715	25.389871725571133	24.450941984323933
82-83	24.296267960146963	25.8939055043457	25.567278873564742	24.2425476619426
84-85	24.342726659668674	25.909695999802025	25.33661510287667	24.410962237652633
86-87	23.979407577312454	26.05473525961316	25.525953067197072	24.439904095877313
88-89	23.986336960295883	25.746751213018808	25.610492010173814	24.65641981651149
90-91	24.262371315658754	25.619271441117693	25.692247452348415	24.426109790875135
92-93	24.29339616318576	25.729984584688832	25.608028335301064	24.368590916824342
94-95	24.310455850047248	25.668200104039336	25.513584660333226	24.50775938558019
96-97	24.045160706565998	25.64277857390146	25.66237017663119	24.649690542901357
98-99	23.921663348205403	25.727062661691207	25.543911439875362	24.807362550228028
100-101	23.966635792977346	25.800342179809636	25.663588835668367	24.569433191544647
102-103	23.81090106706158	25.75135751009513	25.744268684533083	24.693472738310206
104-105	23.98772171137154	25.798789905984687	25.566535580022315	24.646952802621456
106-107	23.97651939647672	25.64958457572229	25.718824103501493	24.65507192429949
108-109	23.9591123058256	25.6824346810218	25.659724450926696	24.698728562225902
110-111	23.779810345956488	26.043043105488294	25.640003773902027	24.537142774653194
112-113	23.773901822440255	26.1771081253372	25.62121731966544	24.427772732557106
114-115	23.877132908736407	26.163826568961944	25.508655364016082	24.450385158285567
116-117	23.722392451034548	26.00299168509511	25.658275118000915	24.61634074586942
118-119	23.764331983073998	25.85201880552393	25.739221806559215	24.64442740484286
120-121	23.73445694863172	25.901687098574556	25.846442966169093	24.517412986624628
122-123	23.737915831498025	26.11899709650013	25.60008413550766	24.543002936494187
124-125	23.607313692642514	26.041465017194387	25.65458157482105	24.696639715342044
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	352.0
1	308.5
2	247.0
3	216.5
4	183.0
5	147.5
6	133.5
7	185.5
8	253.5
9	304.5
10	308.5
11	307.5
12	347.5
13	367.5
14	390.5
15	434.0
16	463.0
17	497.5
18	570.0
19	644.0
20	698.5
21	756.5
22	893.0
23	1076.0
24	1306.0
25	1689.5
26	2307.0
27	3220.0
28	4284.5
29	5889.0
30	8386.5
31	11135.0
32	14541.0
33	18159.0
34	21665.0
35	25464.5
36	29710.5
37	34057.0
38	37715.5
39	41603.5
40	45194.5
41	48259.5
42	52152.5
43	57579.5
44	63377.0
45	68658.5
46	73445.5
47	77607.5
48	82566.5
49	86962.0
50	88573.5
51	89059.0
52	88693.5
53	87026.0
54	85768.5
55	83366.0
56	77968.5
57	71407.5
58	64622.0
59	57264.0
60	48868.0
61	40772.0
62	33498.0
63	25517.5
64	18349.0
65	13586.5
66	10152.0
67	7559.5
68	5617.5
69	4105.0
70	3127.5
71	2316.0
72	1629.0
73	1201.0
74	894.5
75	620.0
76	427.5
77	278.0
78	171.5
79	111.5
80	70.5
81	57.5
82	44.5
83	27.5
84	31.5
85	34.5
86	23.5
87	9.5
88	8.5
89	10.0
90	5.0
91	6.0
92	7.0
93	5.0
94	4.5
95	3.5
96	3.0
97	2.0
98	2.0
99	2.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015104938077486784
2	0.01051674869558807
3	0.00974345835032424
4	0.012424198213905512
5	0.0077329034526382855
6	0.029281927740656976
7	0.012063329386115726
8	0.011857118627378706
9	0.007578245383585521
10-11	0.007062718486742969
12-13	0.005696572210110205
14-15	0.005361479727162545
16-17	0.0038922280711612706
18-19	0.00530992703747829
20-21	0.004794400140635737
22-23	0.00476862379579361
24-25	0.004691294761267227
26-27	0.0037117936572663773
28-29	0.004974834554530631
30-31	0.004562413037056589
32-33	0.006959613107374458
34-35	0.006701849658953181
36-37	0.005902782968847225
38-39	0.005722348554952332
40-41	0.006237875451794884
42-43	0.005980112003373608
44-45	0.006108993727584246
46-47	0.008196877659796584
48-49	0.006985389452216585
50-51	0.004845952830319992
52-53	0.004407754968003824
54-55	0.0052841506926361624
56-57	0.006521415245058288
58-59	0.005799677589478715
60-61	0.0042273205541089294
62-63	0.0046655184164251
64-65	0.0030673850362131867
66-67	0.0033767011743187185
68-69	0.005361479727162545
70-71	0.004098438829898292
72-73	0.0037117936572663773
74-75	0.003479806553687229
76-77	0.0039437807608455255
78-79	0.0028096215877919106
80-81	0.0028096215877919106
82-83	0.0044593076576880785
84-85	0.008196877659796584
86-87	0.006057441037899991
88-89	0.003995333450529781
90-91	0.004356202278319568
92-93	0.006856507728005948
94-95	0.005773901244636587
96-97	0.008067995935585945
98-99	0.005619243175583821
100-101	0.007191600210953606
102-103	0.0046655184164251
104-105	0.004072662485056164
106-107	0.006160546417268502
108-109	0.005593466830741693
110-111	0.00649563890021616
112-113	0.004304649588635313
114-115	0.0021136602770544647
116-117	0.0027580688981076553
118-119	0.15971023264182274
120-121	0.009820787384850625
122-123	0.0018043441389489333
124-125	0.00162390972505404
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1939763.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.4400709230043
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.37054240534502	48.576012830993776
2	10.462416823958545	12.646984297321756
3	3.471020884211214	6.293662452508646
4	1.6700801316860094	4.037590464248111
5	0.9766858127586671	2.951547989631296
6	0.6191797875471837	2.245396216406551
7	0.4436063244696534	1.876811839898738
8	0.3283196283427749	1.587492929796138
9	0.24836252180133203	1.3509943589059836
>10	1.3314241774395807	14.262051392740723
>50	0.06297874622730437	2.571399905867759
>100	0.015041144147591556	1.4334335681746995
>500	2.5620904873626906E-4	0.10700337516024276
>1k	8.540301624542298E-5	0.059618378345431905
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	5.155268968425524E-5
5	0.0	0.0	0.0	0.0	5.155268968425524E-5
6	0.0	0.0	0.0	0.0	5.155268968425524E-5
7	0.0	0.0	0.0	0.0	5.155268968425524E-5
8	0.0	0.0	0.0	0.0	5.155268968425524E-5
9	0.0	0.0	0.0	0.0	5.155268968425524E-5
10-11	0.0	0.0	0.0	5.155268968425524E-5	5.155268968425524E-5
12-13	0.0	0.0	0.0	1.0310537936851048E-4	1.288817242106381E-4
14-15	0.0	0.0	0.0	1.0310537936851048E-4	2.577634484212762E-4
16-17	0.0	0.0	0.0	1.0310537936851048E-4	3.093161381055314E-4
18-19	0.0	0.0	0.0	1.0310537936851048E-4	4.124215174740419E-4
20-21	0.0	0.0	0.0	1.0310537936851048E-4	4.6397420715829716E-4
22-23	0.0	0.0	0.0	1.8043441389489333E-4	5.4130324168468E-4
24-25	0.0	0.0	0.0	4.6397420715829716E-4	5.670795865268077E-4
26-27	0.0	0.0	0.0	7.990666901059563E-4	5.670795865268077E-4
28-29	0.0	0.0	0.0	0.0019332258631595716	5.670795865268077E-4
30-31	0.0	0.0	0.0	0.006676073314111053	5.670795865268077E-4
32-33	0.0	0.0	0.0	0.018790955389911036	6.186322762110628E-4
34-35	0.0	0.0	0.0	0.033767011743187185	6.701849658953181E-4
36-37	0.0	0.0	0.0	0.052119769270782046	6.701849658953181E-4
38-39	0.0	0.0	0.0	0.0875364670838654	6.959613107374457E-4
40-41	0.0	0.0	0.0	0.12991277800432321	7.217376555795733E-4
42-43	0.0	0.0	0.0	0.16940213830246273	7.990666901059563E-4
44-45	0.0	0.0	0.0	0.21329925356860607	8.248430349480838E-4
46-47	0.0	0.0	0.0	0.26356312601075493	8.248430349480838E-4
48-49	0.0	0.0	0.0	0.3173841340411174	8.248430349480838E-4
50-51	0.0	0.0	0.0	0.37486538303906203	8.248430349480838E-4
52-53	0.0	0.0	0.0	0.4385071784542751	9.53724759158722E-4
54-55	0.0	0.0	0.0	0.5096498902185473	0.0010568301385272326
56-57	0.0	0.0	0.0	0.5823134063285051	0.0011083828282114877
58-59	0.0	0.0	0.0	0.6644110646506816	0.001159935517895743
60-61	0.0	0.0	0.0	0.7472820133181219	0.0011857118627378706
62-63	0.0	0.0	0.0	0.8420874096474673	0.0011857118627378706
64-65	0.0	0.0	0.0	0.9336192101818624	0.001211488207579998
66-67	0.0	5.155268968425524E-5	0.0	1.0408745810699553	0.0012372645524221257
68-69	0.0	5.155268968425524E-5	0.0	1.161946072793429	0.0012372645524221257
70-71	0.0	5.155268968425524E-5	0.0	1.287193332381327	0.0012372645524221257
72-73	0.0	5.155268968425524E-5	0.0	1.413033447900594	0.001495028000843402
74-75	0.0	5.155268968425524E-5	0.0	1.543513305491444	0.0015465806905276573
76-77	0.0	5.155268968425524E-5	0.0	1.6983518089581047	0.0015465806905276573
78-79	0.0	5.155268968425524E-5	0.0	1.8698160548479374	0.0015465806905276573
80-81	0.0	5.155268968425524E-5	0.0	2.0442961330842997	0.0016496860698961679
82-83	0.0	5.155268968425524E-5	0.0	2.226947312635616	0.0018043441389489333
84-85	0.0	5.155268968425524E-5	0.0	2.4203987806757836	0.0018043441389489333
86-87	0.0	5.155268968425524E-5	0.0	2.6383893290056566	0.0018558968286331886
88-89	0.0	5.155268968425524E-5	0.0	2.8870279513528203	0.001907449518317444
90-91	0.0	5.155268968425524E-5	0.0	3.1466988492924135	0.001907449518317444
92-93	0.0	5.155268968425524E-5	0.0	3.403946770816847	0.0020105548976859546
94-95	0.0	5.155268968425524E-5	0.0	3.669649333449499	0.0021136602770544647
96-97	0.0	7.732903452638286E-5	0.0	3.9625974925802794	0.00216521296673872
98-99	0.0	1.0310537936851048E-4	0.0	4.2979735153212015	0.00216521296673872
100-101	0.0	1.0310537936851048E-4	0.0	4.65353241607351	0.0022167656564229753
102-103	0.0	1.0310537936851048E-4	0.0	5.027366745318886	0.0022683183461072306
104-105	0.0	1.0310537936851048E-4	0.0	5.41146005981143	0.0022683183461072306
106-107	0.0	1.0310537936851048E-4	0.0	5.826742751562949	0.0022683183461072306
108-109	0.0	1.0310537936851048E-4	0.0	6.2727250700214405	0.0022683183461072306
110-111	0.0	1.0310537936851048E-4	0.0	6.754845823948596	0.0023456473806336134
112-113	0.0	1.0310537936851048E-4	0.0	7.233512547666906	0.0023714237254757412
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1675	0.0	56.841587	1
GTATCAA	3320	0.0	53.232807	1
TCAACGC	3970	0.0	43.46564	4
CAACGCA	4015	0.0	42.680977	5
TATCAAC	4310	0.0	40.312916	2
ATCAACG	4300	0.0	39.991528	3
AACGCAG	4290	0.0	39.94296	6
GTACATG	9920	0.0	37.431217	1
TACATGG	10505	0.0	35.061718	2
ACATGGG	10695	0.0	33.715565	3
ACGCAGA	5320	0.0	32.205486	7
CATGGGG	9660	0.0	31.845789	4
ATGGGGG	5755	0.0	30.5003	5
CGCAGAG	5780	0.0	29.641657	8
GCAGAGT	6695	0.0	25.234482	9
TGGGGGG	4030	0.0	24.212732	6
AGTACAT	7050	0.0	22.619566	2
GAGTACA	7140	0.0	22.585623	1
AGAGTAC	5930	0.0	22.220083	10-11
CAGAGTA	6425	0.0	20.739655	10-11
>>END_MODULE