FastQCFastQC Report
Mon 15 Aug 2016
SRR2921296_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921296_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1939763
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24210.12480906172558194No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24050.068.590611
GTATCAA41550.051.9286161
TATCAAC62550.034.9655462
TCAACGC59250.034.1973234
GTACATG94450.033.854971
CAACGCA61150.033.037325
TACATGG97450.031.9220072
ATCAACG64250.031.9070663
AACGCAG63800.031.6593576
GTGGTAT12100.031.612961
ACATGGG97950.031.394013
CATGGGG92500.028.9916084
ATGGGGG58600.027.4579245
TGGTATC13450.025.6984672
ACGCAGA78850.025.0811427
CGCAGAG83800.023.668268
TGGGGGG41150.022.4432076
AGAGTAC83450.022.23183810-11
GTACTAA4050.022.136391
CAGAGTA88000.021.0490559
Produced by FastQC (version 0.11.5)