##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921296_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1939763
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.090834808169863	33.0	30.0	34.0	16.0	34.0
2	29.882544929457875	33.0	31.0	34.0	26.0	34.0
3	30.113532941910943	33.0	31.0	34.0	26.0	34.0
4	32.22841501771093	37.0	35.0	37.0	19.0	37.0
5	31.8373306429703	37.0	33.0	37.0	19.0	37.0
6	31.98533686847311	37.0	35.0	37.0	17.0	37.0
7	32.22105896441988	37.0	35.0	37.0	17.0	37.0
8	32.4783909168285	37.0	35.0	37.0	17.0	37.0
9	34.23373267765186	39.0	37.0	39.0	17.0	39.0
10-11	34.420485904721346	39.0	37.0	39.0	19.0	39.0
12-13	34.45624774779187	39.0	37.0	39.0	21.5	39.0
14-15	35.87487749792114	40.5	38.0	41.0	24.0	41.0
16-17	35.89600791436892	41.0	38.0	41.0	25.0	41.0
18-19	35.929847357641115	41.0	38.0	41.0	25.0	41.0
20-21	35.91719246114087	41.0	38.0	41.0	24.0	41.0
22-23	35.87594747399554	41.0	38.0	41.0	24.0	41.0
24-25	35.82477060341908	41.0	38.0	41.0	23.5	41.0
26-27	35.728899612993956	40.5	38.0	41.0	22.5	41.0
28-29	35.65260420989574	40.0	37.5	41.0	21.5	41.0
30-31	35.55872109118485	40.0	37.0	41.0	20.0	41.0
32-33	35.43193807697126	40.0	37.0	41.0	18.0	41.0
34-35	35.35449407994688	40.0	37.0	41.0	18.0	41.0
36-37	35.274022908984236	40.0	36.0	41.0	16.0	41.0
38-39	35.180128964208514	40.0	36.0	41.0	15.5	41.0
40-41	35.05155913377047	40.0	36.0	41.0	14.0	41.0
42-43	34.908982437545205	40.0	35.5	41.0	9.0	41.0
44-45	34.75511518675219	40.0	35.0	41.0	7.5	41.0
46-47	34.53798324846901	40.0	35.0	41.0	2.0	41.0
48-49	34.327843917014604	40.0	35.0	41.0	2.0	41.0
50-51	34.14592736329129	40.0	35.0	41.0	2.0	41.0
52-53	33.957830673128626	39.0	35.0	41.0	2.0	41.0
54-55	33.76021503657921	39.0	35.0	41.0	2.0	41.0
56-57	33.51700259258476	39.0	34.5	41.0	2.0	41.0
58-59	33.44847437547783	38.5	35.0	41.0	2.0	41.0
60-61	33.21161038745455	38.0	34.0	41.0	2.0	41.0
62-63	32.92873407730738	37.0	34.0	40.0	2.0	41.0
64-65	32.634489625794494	37.0	34.0	40.0	2.0	41.0
66-67	32.31653506124202	36.0	34.0	39.0	2.0	41.0
68-69	31.98547502968146	36.0	34.0	39.0	2.0	41.0
70-71	31.657867739512508	35.0	33.0	38.5	2.0	40.5
72-73	31.316468042745427	35.0	33.0	37.0	2.0	39.5
74-75	30.987783301362075	35.0	33.0	37.0	2.0	39.0
76-77	30.694639242010492	35.0	33.0	36.0	2.0	39.0
78-79	30.43722093884665	35.0	33.0	36.0	2.0	37.5
80-81	30.207509628753613	35.0	32.5	35.5	2.0	37.0
82-83	30.008715755481468	35.0	32.0	35.0	2.0	37.0
84-85	29.836366865436652	35.0	32.0	35.0	2.0	36.0
86-87	29.692532799109994	35.0	32.0	35.0	2.0	36.0
88-89	29.568588275990418	35.0	32.0	35.0	2.0	36.0
90-91	29.469875443546453	35.0	32.0	35.0	2.0	35.5
92-93	29.4023543082325	35.0	32.0	35.0	2.0	35.0
94-95	29.331492043100113	35.0	32.0	35.0	2.0	35.0
96-97	29.26665499857457	35.0	32.0	35.0	2.0	35.0
98-99	29.210102213517835	35.0	31.0	35.0	2.0	35.0
100-101	29.155192928208237	35.0	31.0	35.0	2.0	35.0
102-103	28.4895262978003	34.0	29.5	35.0	2.0	35.0
104-105	29.02993948229758	35.0	31.0	35.0	2.0	35.0
106-107	29.091550101739234	35.0	31.0	35.0	2.0	35.0
108-109	29.164496899879005	35.0	31.5	35.0	2.0	35.0
110-111	29.272511641886148	35.0	32.0	35.0	2.0	35.0
112-113	29.239444715668874	35.0	32.0	35.0	2.0	35.0
114-115	29.186227389634713	35.0	32.0	35.0	2.0	35.0
116-117	29.132950520243966	35.0	32.0	35.0	2.0	35.0
118-119	29.061191753837967	35.0	32.0	35.0	2.0	35.0
120-121	28.971082292011964	35.0	32.0	35.0	2.0	35.0
122-123	28.86713634603815	35.0	32.0	35.0	2.0	35.0
124-125	28.35551508096608	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	138662.0
3	7917.0
4	5964.0
5	5771.0
6	5872.0
7	6224.0
8	6573.0
9	6792.0
10	6752.0
11	6697.0
12	7651.0
13	8959.0
14	11047.0
15	9508.0
16	6217.0
17	5442.0
18	4868.0
19	4344.0
20	4293.0
21	4361.0
22	4882.0
23	6154.0
24	8122.0
25	10498.0
26	12205.0
27	14424.0
28	17004.0
29	20280.0
30	24314.0
31	30322.0
32	38303.0
33	52289.0
34	79112.0
35	135634.0
36	310344.0
37	669577.0
38	242029.0
39	356.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.830706895543806	18.29482356970325	12.459925720638623	26.41454381411432
2	15.937256252439086	19.58249538732309	38.059855765884805	26.42039259435302
3	19.01624064383123	23.22428049199825	28.582048425503526	29.177430438666992
4	13.524033548277986	16.214536968383232	32.001625233188996	38.259804250149784
5	14.37539606995312	37.156745829870815	32.17195853137907	16.295899568796994
6	33.70783039785607	34.00171337177847	16.578393463811597	15.712062766553869
7	29.73330445678573	31.659473312397836	18.90557884586627	19.701643384950163
8	27.583598849259133	34.77217186871655	17.94418494344253	19.700044338581783
9	27.25116760816003	16.072804925014783	18.323624532067402	38.352402934757784
10-11	26.571254467130373	25.306770447754424	25.527007966081772	22.59496711903343
12-13	26.200670412731704	22.604829684887445	26.498350790869864	24.696149111510987
14-15	23.81221314150234	24.097402620835638	24.022470786379575	28.06791345128245
16-17	23.942899209851927	27.144218133864804	24.599061844153127	24.313820812130142
18-19	24.095340513248267	26.10692646472791	26.29125310669396	23.506479915329862
20-21	24.861774350784092	24.97178779057029	26.190648032775137	23.975789825870482
22-23	24.832904844560908	24.435382054405615	26.033515434617527	24.69819766641595
24-25	24.394011020934002	24.993285262168627	25.95600596567725	24.656697751220126
26-27	24.019506506722728	25.196995715455962	26.158401825377638	24.625095952443672
28-29	24.095005420765318	25.499970872730326	26.018565154609092	24.386458551895256
30-31	24.680025343302248	25.167610682335933	25.908448609443525	24.24391536491829
32-33	24.386149235757152	25.293992101096883	25.86506702107422	24.45479164207174
34-35	24.44997146558626	25.427822883517216	26.076690812228094	24.045514838668435
36-37	24.53487874549623	25.316005099592065	26.089192339476526	24.059923815435187
38-39	24.337921694557533	25.544512396617524	26.083495767266413	24.03407014155853
40-41	24.319208068202148	25.540800602960257	25.964950357337468	24.175040971500124
42-43	24.22814024187491	25.532036645713934	25.7780460808872	24.461777031523955
44-45	24.141480170515674	25.568690608079443	25.98425683962422	24.30557238178066
46-47	24.14972860086516	25.57400053511692	25.601813211201573	24.674457652816347
48-49	24.1991934066172	25.81101402594028	25.412382852956778	24.57740971448574
50-51	24.155786041903056	25.895354226263724	25.553559893657113	24.395299838176108
52-53	24.365992134090607	25.806451612903224	25.485123698101265	24.342432554904903
54-55	24.0143512377543	25.491078033759795	25.748996140250124	24.74557458823578
56-57	24.10021224242343	25.665790099099734	25.457749219878924	24.77624843859791
58-59	24.178778541502236	25.70424840560419	25.645091694191507	24.47188135870207
60-61	24.473118623254493	25.542347183650786	25.792171517860684	24.192362675234037
62-63	24.21543250386774	25.6120464201039	25.757347675978977	24.415173400049387
64-65	24.104645773736276	25.39070494694455	25.72543656106442	24.779212718254755
66-67	24.072167579235195	25.651690438471093	25.584749270916085	24.691392711377627
68-69	23.909905488454005	25.609262574860946	25.72883903858358	24.75199289810147
70-71	23.95846812213657	25.477648558097044	25.79853827503669	24.765345044729692
72-73	24.054356124949287	25.780211293853938	25.472596394507992	24.692836186688787
74-75	23.890805216925987	25.804518387040066	25.588899262435667	24.71577713359828
76-77	23.932459790190862	25.39787077081066	25.610138970585584	25.059530468412895
78-79	23.790380577421054	25.494506287623796	25.759693323359606	24.95541981159554
80-81	24.00172082878166	25.728529722445476	25.434163864348374	24.83558558442449
82-83	24.02731673920989	25.748764153146542	25.54930679675816	24.6746123108854
84-85	24.017547504514727	25.758868480324658	25.374775165832116	24.8488088493285
86-87	23.653817502447463	25.94894324719051	25.488783939068842	24.908455311293185
88-89	23.672582681492532	25.673239462759113	25.553740328071008	25.10043752767735
90-91	23.85872361397659	25.506248717623535	25.593141000062896	25.04188666833698
92-93	23.843481224041497	25.636182310699628	25.602337821616082	24.91799864364279
94-95	23.869725154213626	25.60473316164547	25.445796014507977	25.079745669632924
96-97	23.666745628508334	25.543141906286642	25.509658304873987	25.280454160331033
98-99	23.548997248116006	25.560094485818365	25.450209871107898	25.440698394957735
100-101	23.59125831351562	25.65166466212625	25.56137012614428	25.195706898213853
102-103	23.44067290694791	25.575083141600285	25.600498617614626	25.383745333837176
104-105	23.53288095836475	25.61646077805917	25.50845786533145	25.34220039824463
106-107	23.619789881114098	25.41130109462052	25.561216625364285	25.407692398901098
108-109	23.51671306858677	25.413886138684045	25.600327257908152	25.469073534821028
110-111	23.35693854199666	25.75912273479828	25.53971363916741	25.344225084037653
112-113	23.303827812862483	25.82990076040727	25.56406753447609	25.302203892254155
114-115	23.341474385329093	25.778428489128153	25.447974811284404	25.432122314258354
116-117	23.093164253256152	25.679449145934413	25.586550648369133	25.6408359524403
118-119	23.28126067304805	25.502440382367386	25.59964325437587	25.616655690208702
120-121	23.13504086328161	25.560855177519294	25.75165187876238	25.552452080436716
122-123	23.1437899668614	25.756212713921308	25.49622834494662	25.603768974270675
124-125	22.89781501488714	25.640497556013976	25.49483531867191	25.96685211042698
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	66.0
1	60.0
2	67.0
3	74.5
4	71.0
5	87.5
6	167.0
7	471.0
8	687.0
9	779.0
10	801.5
11	681.0
12	683.0
13	745.0
14	815.0
15	907.0
16	983.5
17	1095.0
18	1202.0
19	1287.0
20	1372.5
21	1469.5
22	1631.0
23	1813.0
24	2022.0
25	2328.0
26	2926.0
27	3803.5
28	4813.0
29	6142.5
30	8090.0
31	10489.0
32	13488.5
33	16875.0
34	20199.0
35	23266.0
36	27320.0
37	31606.0
38	35014.5
39	38989.0
40	43019.0
41	46376.0
42	49914.0
43	55076.5
44	60839.5
45	66775.5
46	71722.0
47	75865.5
48	81433.0
49	86183.0
50	89099.0
51	90828.5
52	90327.5
53	88603.5
54	87178.0
55	84560.0
56	79666.5
57	72784.0
58	65782.5
59	58778.0
60	50287.0
61	41783.5
62	34098.0
63	26295.5
64	19119.5
65	14050.0
66	10493.5
67	7887.5
68	5977.5
69	4455.0
70	3374.0
71	2508.5
72	1851.0
73	1421.5
74	1112.0
75	839.0
76	613.5
77	447.0
78	344.5
79	271.5
80	224.5
81	204.0
82	193.0
83	173.5
84	153.0
85	150.0
86	148.5
87	137.5
88	125.0
89	116.5
90	119.0
91	113.5
92	95.0
93	90.5
94	79.0
95	67.0
96	64.0
97	53.5
98	39.5
99	16.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35004276295609305
2	0.0
3	0.0
4	0.01799188869980508
5	0.18311515375847462
6	0.046191209957092694
7	0.02438442222065273
8	0.0073720346248485
9	0.005877006624005097
10-11	0.0020105548976859546
12-13	0.05704305113562843
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	2.5776344842127623E-4
92-93	4.3819786231616957E-4
94-95	1.2888172421063812E-4
96-97	3.866451726319143E-4
98-99	5.155268968425524E-5
100-101	0.0
102-103	0.0
104-105	2.577634484212762E-5
106-107	2.0621075873702096E-4
108-109	4.3819786231616957E-4
110-111	3.866451726319143E-4
112-113	6.701849658953181E-4
114-115	2.8353979326340383E-4
116-117	5.928559313689353E-4
118-119	2.8353979326340383E-4
120-121	1.0310537936851048E-4
122-123	0.0015981333802119126
124-125	7.732903452638287E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1939763.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.36164971782171
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.91836874325527	52.53844635483317
2	9.109702844895478	11.544116013834223
3	2.977268996090236	5.6593402573800065
4	1.4481977141630342	3.6704078514779304
5	0.8453746954963812	2.6782167668175947
6	0.5698378924512093	2.166352136246117
7	0.37890931169011277	1.6805823357490897
8	0.30442217899637486	1.543095317752346
9	0.21234436813517715	1.2109040525999948
>10	1.1640092214187678	13.006761971121808
>50	0.05608614671348006	2.385910622613092
>100	0.014989454854733388	1.4750142369886687
>500	1.6281061327163502E-4	0.0857771163379538
>1k	3.2562122654327004E-4	0.3550749662480301
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2421	0.12480906172558194	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.155268968425524E-5	0.0
5	0.0	0.0	0.0	5.155268968425524E-5	0.0
6	0.0	0.0	0.0	5.155268968425524E-5	0.0
7	0.0	0.0	0.0	5.155268968425524E-5	0.0
8	0.0	0.0	0.0	5.155268968425524E-5	5.155268968425524E-5
9	0.0	0.0	0.0	5.155268968425524E-5	1.546580690527657E-4
10-11	0.0	0.0	0.0	7.732903452638286E-5	1.546580690527657E-4
12-13	0.0	0.0	0.0	1.546580690527657E-4	1.8043441389489333E-4
14-15	0.0	0.0	0.0	1.546580690527657E-4	2.0621075873702096E-4
16-17	0.0	0.0	0.0	1.546580690527657E-4	2.5776344842127623E-4
18-19	0.0	0.0	0.0	1.546580690527657E-4	3.093161381055314E-4
20-21	0.0	0.0	0.0	1.546580690527657E-4	3.093161381055314E-4
22-23	0.0	0.0	0.0	2.3198710357914858E-4	3.6086882778978667E-4
24-25	0.0	0.0	0.0	4.381978623161695E-4	4.124215174740419E-4
26-27	0.0	0.0	0.0	7.47514000421701E-4	4.124215174740419E-4
28-29	0.0	0.0	0.0	0.001830120483791061	4.124215174740419E-4
30-31	0.0	0.0	0.0	0.006315204486321268	4.124215174740419E-4
32-33	0.0	0.0	0.0	0.01768257256169955	4.6397420715829716E-4
34-35	0.0	0.0	0.0	0.031679127810974844	5.155268968425525E-4
36-37	0.0	0.0	0.0	0.04928437133814801	5.155268968425525E-4
38-39	0.0	5.155268968425524E-5	0.0	0.08209765832217647	5.4130324168468E-4
40-41	0.0	5.155268968425524E-5	0.0	0.12220565089652705	5.670795865268077E-4
42-43	0.0	5.155268968425524E-5	0.0	0.15829253367550572	5.670795865268077E-4
44-45	0.0	5.155268968425524E-5	0.0	0.19917381659512012	5.670795865268077E-4
46-47	0.0	5.155268968425524E-5	0.0	0.24482372331052815	5.670795865268077E-4
48-49	0.0	5.155268968425524E-5	0.0	0.2941596473383604	5.670795865268077E-4
50-51	0.0	5.155268968425524E-5	0.0	0.34702693060956413	5.670795865268077E-4
52-53	0.0	5.155268968425524E-5	0.0	0.40638985278098405	6.18632276211063E-4
54-55	0.0	5.155268968425524E-5	0.0	0.4731763622669367	6.701849658953181E-4
56-57	0.0	5.155268968425524E-5	0.0	0.5412516889949958	6.701849658953181E-4
58-59	0.0	5.155268968425524E-5	0.0	0.6192509084872739	6.701849658953181E-4
60-61	0.0	5.155268968425524E-5	0.0	0.6958324290132352	7.732903452638286E-4
62-63	0.0	5.155268968425524E-5	0.0	0.7845030552701542	8.248430349480838E-4
64-65	0.0	5.155268968425524E-5	0.0	0.8697712040079123	8.248430349480838E-4
66-67	0.0	5.155268968425524E-5	0.0	0.9679790778564186	8.248430349480838E-4
68-69	0.0	5.155268968425524E-5	0.0	1.079745309091884	8.248430349480838E-4
70-71	0.0	7.732903452638286E-5	0.0	1.1961770587437743	8.763957246323391E-4
72-73	0.0	1.0310537936851048E-4	0.0	1.3133305460512443	0.0013145935869485086
74-75	0.0	1.0310537936851048E-4	0.0	1.434608248533455	0.0013919226214748916
76-77	0.0	1.0310537936851048E-4	0.0	1.5803992549605286	0.0013919226214748916
78-79	0.0	1.0310537936851048E-4	0.0	1.7411147650511944	0.0013919226214748916
80-81	2.577634484212762E-5	1.0310537936851048E-4	0.0	1.904433685970915	0.0014692516560012745
82-83	5.155268968425524E-5	1.0310537936851048E-4	0.0	2.073114086617798	0.0015981333802119126
84-85	5.155268968425524E-5	1.0310537936851048E-4	0.0	2.2525689994086906	0.0015981333802119126
86-87	5.155268968425524E-5	1.0310537936851048E-4	0.0	2.454655542970971	0.0016754624147382953
88-89	5.155268968425524E-5	1.0310537936851048E-4	0.0	2.6867457519294886	0.001701238759580423
90-91	5.155268968425524E-5	1.0310537936851048E-4	0.0	2.92785768158275	0.001701238759580423
92-93	5.155268968425524E-5	1.0310537936851048E-4	0.0	3.167551912269695	0.0017527914492646783
94-95	5.155268968425524E-5	1.0310537936851048E-4	0.0	3.4151337044783308	0.0018558968286331886
96-97	5.155268968425524E-5	1.0310537936851048E-4	0.0	3.687074142562777	0.0019590022080016993
98-99	5.155268968425524E-5	1.0310537936851048E-4	0.0	4.001339338877997	0.0020878839322123373
100-101	5.155268968425524E-5	1.0310537936851048E-4	0.0	4.33491101747997	0.0021136602770544647
102-103	5.155268968425524E-5	1.0310537936851048E-4	0.0	4.683922726642378	0.0022167656564229753
104-105	5.155268968425524E-5	1.0310537936851048E-4	0.0	5.041981932844373	0.0022940946909493585
106-107	5.155268968425524E-5	1.546580690527657E-4	0.0	5.431720266857343	0.002319871035791486
108-109	5.155268968425524E-5	1.546580690527657E-4	0.0	5.851513818956233	0.0023456473806336134
110-111	5.155268968425524E-5	1.546580690527657E-4	0.0	6.303991776314941	0.0025260817945285067
112-113	5.155268968425524E-5	1.546580690527657E-4	0.0	6.754716942224386	0.0026291871738970173
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2405	0.0	68.59061	1
GTATCAA	4155	0.0	51.928616	1
TATCAAC	6255	0.0	34.965546	2
TCAACGC	5925	0.0	34.197323	4
GTACATG	9445	0.0	33.85497	1
CAACGCA	6115	0.0	33.03732	5
TACATGG	9745	0.0	31.922007	2
ATCAACG	6425	0.0	31.907066	3
AACGCAG	6380	0.0	31.659357	6
GTGGTAT	1210	0.0	31.61296	1
ACATGGG	9795	0.0	31.39401	3
CATGGGG	9250	0.0	28.991608	4
ATGGGGG	5860	0.0	27.457924	5
TGGTATC	1345	0.0	25.698467	2
ACGCAGA	7885	0.0	25.081142	7
CGCAGAG	8380	0.0	23.66826	8
TGGGGGG	4115	0.0	22.443207	6
AGAGTAC	8345	0.0	22.231838	10-11
GTACTAA	405	0.0	22.13639	1
CAGAGTA	8800	0.0	21.049055	9
>>END_MODULE