FastQCFastQC Report
Mon 15 Aug 2016
SRR2921295_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921295_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162351
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22550.057.266911
GTATCAA38700.054.5893551
TCAACGC49050.041.490394
CAACGCA50300.040.5766835
AACGCAG53250.038.5513736
TATCAAC53850.038.1235962
ATCAACG54250.037.513433
GTACATG111150.035.7649461
TACATGG116650.033.5151442
ACATGGG121450.031.6515833
ACGCAGA65650.030.9921267
CATGGGG110750.030.5723614
ATGGGGG70100.028.945885
CGCAGAG70750.028.8421528
TGGGGGG50550.025.1902776
GCAGAGT80550.025.1836519
AGAGTAC71200.022.60045210-11
GTGGTAT12500.021.8997121
CAGAGTA77650.021.25941810-11
GTGTAGG8700.020.5207211
Produced by FastQC (version 0.11.5)