FastQCFastQC Report
Mon 15 Aug 2016
SRR2921295_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921295_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162351
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28420.1314310211431909No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27950.067.3446961
GTATCAA47900.052.5195731
GTACATG106850.037.245551
TCAACGC66800.036.13394
TATCAAC68850.035.9236452
TACATGG112650.035.6586572
CAACGCA67950.034.9961055
ATCAACG70050.034.5425343
ACATGGG116900.034.0053753
GTGGTAT13500.033.639881
AACGCAG71150.032.8388336
CATGGGG106350.031.7747294
ATGGGGG71550.028.0709115
ACGCAGA86750.026.445217
TGGTATC15700.025.433812
CGCAGAG90600.025.3807378
TGGGGGG51500.023.262986
GTAAGTA11850.023.1353322
AGAGTAC92100.022.79919410-11
GCAGAGT100500.021.9908969
Produced by FastQC (version 0.11.5)