FastQCFastQC Report
Mon 15 Aug 2016
SRR2921294_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921294_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493240
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17700.063.2080961
GTATCAA32150.054.2343941
TCAACGC41300.041.4983254
CAACGCA41950.040.9958085
TATCAAC44200.038.775582
AACGCAG44250.038.7252856
ATCAACG45100.037.869843
GTACATG89450.037.854721
TACATGG91000.036.6213842
ACATGGG95450.034.539973
CATGGGG84700.033.162464
ATGGGGG56000.031.985425
ACGCAGA54400.031.3852527
CGCAGAG57850.029.4106988
TGGGGGG43100.027.748166
GCAGAGT64300.025.9970139
TATACCG1701.3977153E-524.5031915
GTGGTAT9750.024.4142551
AGAGTAC57850.023.59975210-11
CAGAGTA61600.022.83907510-11
Produced by FastQC (version 0.11.5)