##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921294_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1493240
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.23273686748279	31.0	31.0	34.0	26.0	34.0
2	30.185124293482627	31.0	31.0	34.0	26.0	34.0
3	30.299525863223593	33.0	31.0	34.0	26.0	34.0
4	33.7123088050146	37.0	35.0	37.0	28.0	37.0
5	33.743234175350246	37.0	35.0	37.0	29.0	37.0
6	33.888418472583105	37.0	35.0	37.0	30.0	37.0
7	33.7495908226407	37.0	35.0	37.0	29.0	37.0
8	33.76977378050414	37.0	35.0	37.0	29.0	37.0
9	35.35669952586322	39.0	37.0	39.0	29.0	39.0
10-11	35.37799918298465	39.0	37.0	39.0	29.0	39.0
12-13	35.2962929602743	39.0	37.0	39.0	28.5	39.0
14-15	36.57618299804452	40.5	38.0	41.0	28.0	41.0
16-17	36.5624380541641	40.0	38.0	41.0	28.0	41.0
18-19	36.55819124856018	40.0	38.0	41.0	28.0	41.0
20-21	36.62247160536819	40.5	38.0	41.0	28.0	41.0
22-23	36.56966897484664	40.0	38.0	41.0	28.0	41.0
24-25	36.57042404435991	40.0	38.0	41.0	28.0	41.0
26-27	36.559322011197125	40.0	38.0	41.0	29.0	41.0
28-29	36.49966750154027	40.0	38.0	41.0	29.0	41.0
30-31	36.41688208191583	40.0	37.0	41.0	29.0	41.0
32-33	36.345771945568025	40.0	37.0	41.0	29.0	41.0
34-35	36.23301244274195	40.0	37.0	41.0	28.0	41.0
36-37	36.10814236157617	40.0	37.0	41.0	27.0	41.0
38-39	36.00318401596529	40.0	36.5	41.0	27.0	41.0
40-41	35.89545987249203	40.0	36.0	41.0	27.0	41.0
42-43	35.67756556213335	40.0	35.5	41.0	26.0	41.0
44-45	35.38245124695294	40.0	35.0	41.0	25.0	41.0
46-47	35.14096293964801	40.0	35.0	41.0	24.0	41.0
48-49	34.91840025715893	39.0	35.0	41.0	23.5	41.0
50-51	34.737875358281315	39.0	35.0	41.0	23.0	41.0
52-53	34.54701655460609	39.0	35.0	41.0	22.5	41.0
54-55	34.346885966087164	39.0	34.0	40.5	22.0	41.0
56-57	34.10911507862099	38.0	34.0	40.0	21.5	41.0
58-59	33.840321716535854	38.0	34.0	40.0	20.0	41.0
60-61	33.55278287482253	37.5	34.0	40.0	18.5	41.0
62-63	33.22041868688222	37.0	33.0	40.0	17.5	41.0
64-65	32.87761511880207	36.0	33.0	39.5	14.0	41.0
66-67	32.52909813559776	36.0	33.0	39.0	12.0	41.0
68-69	32.16804063646835	35.0	32.5	39.0	8.0	40.0
70-71	32.12056735688838	35.0	33.0	38.5	8.0	40.0
72-73	31.830722790710134	35.0	33.0	37.0	7.0	39.5
74-75	31.501191703945782	35.0	32.5	37.0	3.5	39.0
76-77	31.19811115426857	35.0	32.0	36.5	2.0	39.0
78-79	30.913786129490237	35.0	32.0	36.0	2.0	37.5
80-81	30.641601484021322	35.0	32.0	35.5	2.0	37.0
82-83	30.4238612011465	35.0	32.0	35.0	2.0	37.0
84-85	30.216226795424717	35.0	32.0	35.0	2.0	36.0
86-87	30.05087829150036	35.0	31.5	35.0	2.0	36.0
88-89	29.907007580830946	35.0	31.0	35.0	2.0	36.0
90-91	29.78879550507621	35.0	31.0	35.0	2.0	35.0
92-93	29.677352267552436	35.0	31.0	35.0	2.0	35.0
94-95	29.553243952747046	35.0	31.0	35.0	2.0	35.0
96-97	29.460227090085986	35.0	31.0	35.0	2.0	35.0
98-99	29.378963528970562	35.0	31.0	35.0	2.0	35.0
100-101	29.282105019956603	34.0	31.0	35.0	2.0	35.0
102-103	28.428221853151534	33.5	29.0	34.5	2.0	35.0
104-105	29.023368982882857	34.0	30.0	35.0	2.0	35.0
106-107	29.103248305697676	34.0	31.0	35.0	2.0	35.0
108-109	29.040274168921272	34.0	30.0	35.0	2.0	35.0
110-111	28.958804010072058	34.0	30.0	35.0	2.0	35.0
112-113	28.869565508558573	34.0	30.0	35.0	2.0	35.0
114-115	28.740067906029843	34.0	30.0	35.0	2.0	35.0
116-117	28.598552811336425	34.0	30.0	35.0	2.0	35.0
118-119	28.59419182448903	34.0	30.0	35.0	2.0	35.0
120-121	28.755041721357586	35.0	31.0	35.0	2.0	35.0
122-123	28.62111984677614	35.0	31.0	35.0	2.0	35.0
124-125	28.037729701856364	34.5	29.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61281.0
3	8277.0
4	5151.0
5	4409.0
6	4656.0
7	5327.0
8	5429.0
9	5713.0
10	6326.0
11	7187.0
12	7618.0
13	7764.0
14	8028.0
15	7108.0
16	4992.0
17	4481.0
18	4251.0
19	4050.0
20	4005.0
21	4223.0
22	4814.0
23	6311.0
24	9470.0
25	11963.0
26	12038.0
27	13218.0
28	16054.0
29	19028.0
30	23182.0
31	29037.0
32	38524.0
33	52370.0
34	85305.0
35	137224.0
36	279184.0
37	463673.0
38	121532.0
39	37.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.02988373635118	18.355162071178164	12.986056131805658	24.628898060665
2	16.131184169811245	19.901396206145023	39.656581243980426	24.310838380063306
3	20.144347946062414	24.424219002731977	28.348779251188333	27.08265380001728
4	13.49500204954816	15.38165801748412	34.93863411680549	36.18470581616223
5	14.198101802892102	38.584194907109435	32.20639461094728	15.011308679051183
6	33.41613408899072	34.37129037876656	17.477390570360544	14.735184961882176
7	29.420889348500516	32.51496268036929	19.359257036612355	18.704890934517845
8	27.729308825144738	35.44323975320351	18.675504938876877	18.151946482774882
9	28.168323595361134	16.47525590640831	18.286759840441917	37.06966065778864
10-11	26.518813995159835	26.168677151796178	25.946760849101935	21.365748003942052
12-13	26.1264703410277	23.46678846745652	26.562997585303805	23.843743606211973
14-15	24.03454689018383	24.718834363922397	23.967407355814082	27.279211390079688
16-17	24.299263517635865	27.713690053235414	24.522311122778984	23.46473530634974
18-19	24.202837547730294	27.087098430136713	25.99893313188077	22.71113089025222
20-21	25.160162821598103	25.768734663480597	25.95883383517461	23.112268679746688
22-23	25.287425987849986	25.09093088716801	25.67452267562838	23.947120449353623
24-25	24.713377289181036	25.32570077653843	25.76490207043428	24.19601986384626
26-27	24.034200821668914	25.83053421873645	25.911234442185656	24.22403051740898
28-29	24.157685449507337	26.464333252408895	25.69084074908123	23.687140549002535
30-31	25.138430504805886	25.694697448646096	25.719242576856722	23.447629469691286
32-33	24.65565028877055	25.726748420527066	25.638076256052262	23.97952503465012
34-35	24.507723491341565	26.17776168938	25.896039854280197	23.418474964998246
36-37	24.516726383819442	26.16183906506379	25.803937983457793	23.517496567658977
38-39	24.587416418422237	26.249638348942373	25.582459120036006	23.580486112599385
40-41	24.540476971024038	25.87808403902752	25.730476780152582	23.85096220979586
42-43	24.20110605652173	25.962558831457816	25.539759266111574	24.29657584590888
44-45	23.93721844254294	26.001417812262773	25.998236608555565	24.063127136638727
46-47	23.89986916539443	25.78416208086965	25.88177735479686	24.434191398939056
48-49	23.904152066523075	26.150799391348144	25.88458117679836	24.060467365330418
50-51	24.060353557000955	26.170430922709297	25.84668972279074	23.92252579749901
52-53	24.212748715819366	25.98856794604767	25.74325093592826	24.055432402204705
54-55	23.70204626703939	25.75343667582399	26.193745069175534	24.350771987961085
56-57	23.981496798372834	25.895039135463556	25.63880180585033	24.484662260313282
58-59	24.111467665181774	26.11742754665387	25.82539402424605	23.945710763918306
60-61	24.649814170709796	26.05896241657438	25.855871211043073	23.43535220167275
62-63	24.315765504816948	25.989626062759307	25.919874896612228	23.774733535811514
64-65	23.97518868167771	25.656689924487146	25.98417682417403	24.383944569661118
66-67	24.072276399868873	26.001374913816743	25.699938621114804	24.226410065199577
68-69	23.86502946277196	26.015408308109905	25.923823741063806	24.195738488054324
70-71	23.867581584488367	25.843838904456007	26.103185424892978	24.18539408616265
72-73	23.944210473796424	26.475680986295387	25.553255845647904	24.026852694260285
74-75	23.900111540059825	26.35847990755335	25.674840801762404	24.066567750624422
76-77	23.7827305250948	25.801009658593955	25.74770054206531	24.668559274245936
78-79	23.775418256240734	25.900025683208504	25.939036032503488	24.38552002804727
80-81	24.124208993746958	26.259666365968766	25.466131973562696	24.14999266672158
82-83	24.208729359321925	26.247046548010672	25.761669149084366	23.78255494358304
84-85	24.085637016815838	26.34835471590955	25.404623619330312	24.1613846479443
86-87	23.588511122637833	26.716934049091567	25.525659370116234	24.168895458154363
88-89	23.590536943091063	26.174594404540656	25.825539538232288	24.409329114135986
90-91	24.115681441390276	25.826572267917342	25.835111151145263	24.22263513954712
92-93	24.33106784022246	26.00363264721129	25.62928379788444	24.036015714681817
94-95	24.149190487368497	26.09453896674249	25.535419547179238	24.220850998709775
96-97	23.76909852100946	25.995386819349907	25.68800887809563	24.547505781545002
98-99	23.428703077078143	26.07843065029818	25.648468158163247	24.844398114460436
100-101	23.592459451721304	26.33070518090384	25.689802110000652	24.387033257374203
102-103	23.537798982499297	26.258791288458067	25.792869324527008	24.410540404515633
104-105	23.74828804393323	26.2290086560517	25.5699767274432	24.452726572571866
106-107	23.748293027918997	26.048678590970308	25.832791410858054	24.37023697025264
108-109	23.719074157727782	26.0610046489013	25.73052847724121	24.48939271612971
110-111	23.42150232078581	26.737508593487604	25.66161901473844	24.179370070988153
112-113	23.36906602427519	26.857582573456952	25.62520489120298	24.148146511064876
114-115	23.54792111459653	26.911125338533783	25.448398209487788	24.0925553373819
116-117	23.388356250619477	26.753587606717407	25.506363531646215	24.351692611016908
118-119	23.292012331236986	26.441662731546074	25.824094743394365	24.442230193822574
120-121	23.326613443352	26.522523910306212	25.939768800064293	24.211093846277493
122-123	23.363373780897383	26.81155149747959	25.634020421789582	24.191054299833446
124-125	23.194946996774064	26.61080717996318	25.566781921644026	24.627463901618725
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	593.0
1	503.0
2	389.5
3	319.5
4	246.0
5	199.0
6	224.5
7	309.0
8	389.0
9	446.0
10	453.0
11	465.5
12	519.5
13	552.0
14	568.0
15	632.0
16	710.5
17	787.0
18	857.0
19	908.5
20	960.5
21	1069.0
22	1173.0
23	1348.5
24	1625.0
25	2014.5
26	2735.5
27	3672.5
28	4519.5
29	5700.5
30	7505.5
31	9379.5
32	11785.0
33	14723.5
34	17650.0
35	20392.5
36	23669.0
37	27170.0
38	29727.5
39	32542.5
40	35344.5
41	37901.0
42	40490.5
43	43881.5
44	48069.5
45	51865.0
46	55964.5
47	60175.5
48	64339.0
49	67789.5
50	69272.5
51	69498.5
52	68453.5
53	66832.5
54	65447.5
55	62588.5
56	57733.5
57	52096.0
58	46982.0
59	40818.5
60	34089.0
61	28950.0
62	23962.0
63	18315.5
64	13252.5
65	9893.0
66	7488.5
67	5594.5
68	4250.0
69	3155.5
70	2408.0
71	1837.0
72	1376.5
73	1091.0
74	829.0
75	589.0
76	416.5
77	285.0
78	201.0
79	146.0
80	95.5
81	60.5
82	53.0
83	36.5
84	30.5
85	26.5
86	21.0
87	22.0
88	16.0
89	12.0
90	10.0
91	9.5
92	7.0
93	4.0
94	3.5
95	4.0
96	4.0
97	3.5
98	2.5
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017009991695909565
2	0.012991883421285258
3	0.012121293295116659
4	0.015268811443572367
5	0.009308617502879644
6	0.033484235621869224
7	0.012857946478797783
8	0.012590072593822828
9	0.00776834266427366
10-11	0.00733304760118936
12-13	0.006127615118802068
14-15	0.005424446170742814
16-17	0.004085076745868045
18-19	0.005658835820095898
20-21	0.004821729929549168
22-23	0.004888698400792907
24-25	0.005022635343280383
26-27	0.0039846240390024375
28-29	0.00519005652138973
30-31	0.004821729929549168
32-33	0.0066633628887519765
34-35	0.006730331359995714
36-37	0.00602716241193646
38-39	0.005893225469448983
40-41	0.005926709705070853
42-43	0.006529425946264499
44-45	0.006328520532533283
46-47	0.008940290911039083
48-49	0.006964721009348798
50-51	0.004821729929549168
52-53	0.004687792987061692
54-55	0.0054914146419865525
56-57	0.006228067825667675
58-59	0.005993678176314591
60-61	0.004587340280196084
62-63	0.005022635343280383
64-65	0.0033819077978087917
66-67	0.003716750154027484
68-69	0.005658835820095898
70-71	0.004285982159599261
72-73	0.0037837186252712225
74-75	0.0036497816827837453
76-77	0.0041520452171117835
78-79	0.0031140339128338376
80-81	0.003147518148455707
82-83	0.004821729929549168
84-85	0.008304090434223567
86-87	0.006127615118802068
88-89	0.004352950630842999
90-91	0.00472127722268356
92-93	0.007634405721786183
94-95	0.005926709705070853
96-97	0.008504995847954782
98-99	0.0057927727625833765
100-101	0.007533953014920575
102-103	0.004788245693927299
104-105	0.004352950630842999
106-107	0.006629878653130106
108-109	0.006127615118802068
110-111	0.006931236773726929
112-113	0.004654308751439822
114-115	0.002410864964774584
116-117	0.0029466127347244917
118-119	0.16018858321502236
120-121	0.010714955398998152
122-123	0.0020760226085558917
124-125	0.001942085666068415
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1493240.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.21497794356853
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.81073635981446	51.71663894532771
2	9.918726014061717	12.540240924144216
3	3.1766688280140056	6.024391496907812
4	1.5373264701205334	3.8872823560293432
5	0.8957843345879793	2.831349347658666
6	0.5468562625381791	2.0741703944791983
7	0.3979329353011099	1.7608725209655403
8	0.27757832601892746	1.4037686205519337
9	0.21993778848753728	1.2513026203377218
>10	1.1572598082871366	12.828118443078756
>50	0.04753916770717092	2.0316859720640315
>100	0.012912860436301845	1.2519015965257374
>500	5.29174732101484E-4	0.2390463414032647
>1k	2.116698928405936E-4	0.1592304205261041
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.696847124373845E-5	1.339369424874769E-4
10-11	0.0	0.0	0.0	6.696847124373845E-5	1.339369424874769E-4
12-13	0.0	0.0	0.0	1.0045270686560767E-4	1.339369424874769E-4
14-15	0.0	0.0	0.0	1.339369424874769E-4	2.3438964935308456E-4
16-17	0.0	0.0	0.0	1.339369424874769E-4	4.0181082746243066E-4
18-19	0.0	0.0	0.0	1.339369424874769E-4	4.0181082746243066E-4
20-21	0.0	0.0	0.0	1.339369424874769E-4	4.0181082746243066E-4
22-23	0.0	0.0	0.0	3.3484235621869226E-4	4.0181082746243066E-4
24-25	0.0	0.0	0.0	5.692320055717768E-4	4.0181082746243066E-4
26-27	0.0	0.0	0.0	0.0012724009536310304	4.0181082746243066E-4
28-29	0.0	0.0	0.0	0.0025782861428839303	4.0181082746243066E-4
30-31	0.0	0.0	0.0	0.010681471163376283	4.687792987061691E-4
32-33	0.0	0.0	0.0	0.02769146285928585	6.696847124373845E-4
34-35	0.0	0.0	0.0	0.047346709169323084	6.696847124373845E-4
36-37	0.0	0.0	0.0	0.07885537488950202	6.696847124373845E-4
38-39	0.0	0.0	0.0	0.13005277115534006	7.031689480592537E-4
40-41	0.0	0.0	0.0	0.1908266588090327	7.366531836811229E-4
42-43	0.0	0.0	0.0	0.24835257560740404	7.366531836811229E-4
44-45	0.0	0.0	0.0	0.31110203316278695	7.366531836811229E-4
46-47	0.0	0.0	0.0	0.37957729500950954	7.366531836811229E-4
48-49	0.0	0.0	0.0	0.45170233853901587	8.036216549248613E-4
50-51	0.0	0.0	0.0	0.5281803326993652	8.371058905467306E-4
52-53	0.0	0.0	0.0	0.6103506469154323	0.0010045270686560768
54-55	0.0	0.0	0.0	0.6985816077790576	0.0011384640111435536
56-57	0.0	0.0	0.0	0.7897591813774075	0.0011384640111435536
58-59	0.0	0.0	0.0	0.8951005866438082	0.0011384640111435536
60-61	0.0	0.0	0.0	1.0082103345744824	0.0011384640111435536
62-63	0.0	0.0	0.0	1.1285861616351023	0.0011384640111435536
64-65	0.0	0.0	0.0	1.2449773646567195	0.0011384640111435536
66-67	0.0	0.0	0.0	1.3791821810291713	0.0011384640111435536
68-69	0.0	0.0	0.0	1.524001500093756	0.0011384640111435536
70-71	0.0	0.0	0.0	1.6795022903217165	0.0011384640111435536
72-73	0.0	0.0	0.0	1.8291098550802283	0.001205432482387292
74-75	0.0	0.0	0.0	1.9904369023063941	0.001205432482387292
76-77	0.0	0.0	0.0	2.1696110471190164	0.001205432482387292
78-79	0.0	0.0	0.0	2.380628700008036	0.001205432482387292
80-81	0.0	0.0	0.0	2.5854182850713885	0.001205432482387292
82-83	0.0	0.0	0.0	2.801960836838017	0.001339369424874769
84-85	0.0	0.0	0.0	3.026171278562053	0.001339369424874769
86-87	0.0	0.0	0.0	3.272213441911548	0.001339369424874769
88-89	0.0	0.0	0.0	3.5555570437438053	0.001339369424874769
90-91	0.0	0.0	0.0	3.847506094130883	0.001339369424874769
92-93	0.0	0.0	0.0	4.130682274785031	0.0014063378961185074
94-95	0.0	0.0	0.0	4.424272052717581	0.0014733063673622458
96-97	0.0	0.0	0.0	4.755531595724733	0.0014733063673622458
98-99	0.0	0.0	0.0	5.131894404114543	0.0014733063673622458
100-101	0.0	0.0	0.0	5.532499799094587	0.0014733063673622458
102-103	0.0	0.0	0.0	5.953564062039591	0.0014733063673622458
104-105	0.0	0.0	0.0	6.390399399962497	0.0014733063673622458
106-107	0.0	0.0	0.0	6.863565133535132	0.0016072433098497227
108-109	0.0	0.0	0.0	7.35903136803193	0.0017076960167153304
110-111	0.0	0.0	0.0	7.888484101684926	0.0017411802523371997
112-113	0.0	0.0	0.0	8.41432053789076	0.0017411802523371997
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1770	0.0	63.208096	1
GTATCAA	3215	0.0	54.234394	1
TCAACGC	4130	0.0	41.498325	4
CAACGCA	4195	0.0	40.995808	5
TATCAAC	4420	0.0	38.77558	2
AACGCAG	4425	0.0	38.725285	6
ATCAACG	4510	0.0	37.86984	3
GTACATG	8945	0.0	37.85472	1
TACATGG	9100	0.0	36.621384	2
ACATGGG	9545	0.0	34.53997	3
CATGGGG	8470	0.0	33.16246	4
ATGGGGG	5600	0.0	31.98542	5
ACGCAGA	5440	0.0	31.385252	7
CGCAGAG	5785	0.0	29.410698	8
TGGGGGG	4310	0.0	27.74816	6
GCAGAGT	6430	0.0	25.997013	9
TATACCG	170	1.3977153E-5	24.503191	5
GTGGTAT	975	0.0	24.414255	1
AGAGTAC	5785	0.0	23.599752	10-11
CAGAGTA	6160	0.0	22.839075	10-11
>>END_MODULE