FastQCFastQC Report
Mon 15 Aug 2016
SRR2921294_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921294_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493240
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33130.22186654523050547No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23980.1605903940424848No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23890.15998767780129117No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25350.071.1973341
GTATCAA42850.050.3490371
GTACATG85950.038.5906331
TACATGG88900.036.9405062
ACATGGG91100.035.9829983
TATCAAC62300.034.0576742
CATGGGG81850.033.6415754
TCAACGC61700.033.6160854
CAACGCA62850.033.095825
ATCAACG63950.032.6197433
ATGGGGG51450.031.972515
AACGCAG65000.031.7122046
TGGTATC11300.026.3720932
TGGGGGG42300.026.1960756
ACGCAGA78500.026.1023527
CGCAGAG82750.024.6864558
GTGGTAT12350.024.6795961
CGTATAC1451.3250049E-424.6624553
GTAAGTA9700.024.5777022
AGAGTAC82150.023.45226310-11
Produced by FastQC (version 0.11.5)