##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921294_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1493240
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.85221933513702	31.0	30.0	34.0	16.0	34.0
2	29.65574254640915	33.0	31.0	34.0	25.0	34.0
3	29.884994374648414	33.0	31.0	34.0	25.0	34.0
4	31.95549476306555	37.0	33.0	37.0	19.0	37.0
5	31.57091425356942	37.0	33.0	37.0	17.0	37.0
6	31.714541533845864	37.0	33.0	37.0	17.0	37.0
7	31.953366505049424	37.0	35.0	37.0	17.0	37.0
8	32.2107906297715	37.0	35.0	37.0	17.0	37.0
9	33.92824194369291	39.0	35.0	39.0	17.0	39.0
10-11	34.119441616886775	39.0	36.0	39.0	17.0	39.0
12-13	34.14870683882029	39.0	37.0	39.0	17.0	39.0
14-15	35.51978851356782	40.0	37.0	41.0	17.0	41.0
16-17	35.54413054833785	40.0	37.0	41.0	17.5	41.0
18-19	35.586799509790794	40.0	37.5	41.0	18.0	41.0
20-21	35.577138303286816	40.0	37.5	41.0	18.0	41.0
22-23	35.53033035546865	40.0	37.0	41.0	18.0	41.0
24-25	35.4678604912807	40.5	37.0	41.0	18.0	41.0
26-27	35.36265771074978	40.0	37.0	41.0	17.0	41.0
28-29	35.270825185502666	40.0	37.0	41.0	16.0	41.0
30-31	35.15098611073907	40.0	36.0	41.0	14.5	41.0
32-33	34.996955278454905	40.0	35.5	41.0	9.5	41.0
34-35	34.90571810291715	40.0	35.0	41.0	9.0	41.0
36-37	34.8181189895797	40.0	35.0	41.0	8.0	41.0
38-39	34.71872773298331	40.0	35.0	41.0	5.0	41.0
40-41	34.58067892636147	40.0	35.0	41.0	2.0	41.0
42-43	34.418846267177415	40.0	35.0	41.0	2.0	41.0
44-45	34.24031100158045	40.0	35.0	41.0	2.0	41.0
46-47	33.97868795371139	40.0	35.0	41.0	2.0	41.0
48-49	33.72610564946024	40.0	35.0	41.0	2.0	41.0
50-51	33.531960033216365	39.5	35.0	41.0	2.0	41.0
52-53	33.35172343360746	39.0	34.0	41.0	2.0	41.0
54-55	33.15558751439822	39.0	34.0	41.0	2.0	41.0
56-57	32.92030818890466	38.5	34.0	41.0	2.0	41.0
58-59	32.859856419597655	38.5	34.0	41.0	2.0	41.0
60-61	32.626358790281536	37.5	34.0	41.0	2.0	41.0
62-63	32.33775180145187	37.0	34.0	40.0	2.0	41.0
64-65	32.049916289410945	36.5	33.0	40.0	2.0	41.0
66-67	31.741474913610674	36.0	33.0	39.0	2.0	41.0
68-69	31.414459162626237	35.5	33.0	39.0	2.0	41.0
70-71	31.086640459671585	35.0	33.0	38.5	2.0	40.5
72-73	30.74800467439929	35.0	32.5	37.0	2.0	39.5
74-75	30.417020036966598	35.0	32.0	37.0	2.0	39.0
76-77	30.120844606359327	35.0	32.0	36.0	2.0	39.0
78-79	29.86682683292706	35.0	32.0	36.0	2.0	37.5
80-81	29.63659425142643	35.0	32.0	35.5	2.0	37.0
82-83	29.428236921057568	35.0	31.5	35.0	2.0	37.0
84-85	29.255099314242855	35.0	31.0	35.0	2.0	36.0
86-87	29.115571508933595	35.0	31.0	35.0	2.0	36.0
88-89	28.990360558249176	35.0	31.0	35.0	2.0	36.0
90-91	28.887864308483564	35.0	31.0	35.0	2.0	35.5
92-93	28.8121259810881	35.0	31.0	35.0	2.0	35.0
94-95	28.741477257507164	35.0	31.0	35.0	2.0	35.0
96-97	28.67444483137339	35.0	31.0	35.0	2.0	35.0
98-99	28.621818662773563	35.0	31.0	35.0	2.0	35.0
100-101	28.56770445474271	35.0	31.0	35.0	2.0	35.0
102-103	27.913846401114355	34.0	29.0	35.0	2.0	35.0
104-105	28.43728938415794	35.0	30.0	35.0	2.0	35.0
106-107	28.491138397042672	35.0	31.0	35.0	2.0	35.0
108-109	28.559849722750528	35.0	31.0	35.0	2.0	35.0
110-111	28.66319613725858	35.0	31.0	35.0	2.0	35.0
112-113	28.62384713776754	35.0	31.0	35.0	2.0	35.0
114-115	28.565572513460662	35.0	31.0	35.0	2.0	35.0
116-117	28.50482775709196	35.0	31.0	35.0	2.0	35.0
118-119	28.42938509549704	35.0	31.0	35.0	2.0	35.0
120-121	28.332477029814363	35.0	31.0	35.0	2.0	35.0
122-123	28.215360558249174	35.0	31.0	35.0	2.0	35.0
124-125	27.701445514451798	34.5	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	113131.0
3	7383.0
4	5452.0
5	5217.0
6	5525.0
7	5699.0
8	5753.0
9	5610.0
10	5802.0
11	6107.0
12	6856.0
13	8770.0
14	11169.0
15	9807.0
16	5915.0
17	5032.0
18	4454.0
19	3832.0
20	3692.0
21	3648.0
22	4170.0
23	5073.0
24	6786.0
25	8782.0
26	10229.0
27	11227.0
28	13435.0
29	15612.0
30	19010.0
31	23753.0
32	29986.0
33	40863.0
34	62735.0
35	107287.0
36	227384.0
37	496829.0
38	180966.0
39	259.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.74566044735245	18.382539235577443	12.712844565083714	26.15895575198639
2	15.737054994508584	19.76353432803836	38.50064289732394	25.998767780129118
3	18.93935335244167	23.82309608636254	28.88691703945782	28.35063352173797
4	13.401092342749665	16.37295051770402	31.8522939331707	38.37366320637562
5	13.943735017440655	37.98506926949183	31.89831752525859	16.172878187808916
6	33.26070161967382	34.18387482151962	16.724323114640853	15.83110044416571
7	29.267729020148725	32.617096571722925	18.73397778341949	19.381196624708863
8	27.875712352079574	34.932560542810776	17.7857461940357	19.405980911073947
9	27.868038900650106	16.032822172379362	18.211590764946553	37.88754816202398
10-11	26.68518604711124	25.58668051155547	25.172338394352305	22.555795046980982
12-13	26.32572204294735	23.009993085112086	26.283509064399585	24.38077580754098
14-15	23.976721759395677	24.324756904449384	24.248446331467143	27.45007500468779
16-17	23.755926709705072	27.22465913048137	24.805121748680723	24.21429241113284
18-19	23.76844981382765	26.19110122954113	26.844746993115642	23.195701963515578
20-21	24.641718678846	24.95613565133535	26.976306554873965	23.425839114944687
22-23	24.609573812649003	24.335170501727788	26.731101497415015	24.32415418820819
24-25	24.098637861294904	24.84560418954756	26.66972489352013	24.38603305563741
26-27	23.477806648629826	25.117027403498433	26.989934638772066	24.415231309099674
28-29	23.66026224853339	25.68736438884573	26.64263614690204	24.00973721571884
30-31	24.606526747207415	25.09730518871715	26.63620717366264	23.659960890412794
32-33	24.1917240363237	25.152554177493236	26.622646058235784	24.03307572794728
34-35	24.159746591304813	25.453878813854438	26.90083308778227	23.48554150705848
36-37	24.1617221612065	25.322553641745465	26.94627119552115	23.56945300152688
38-39	24.109453269400767	25.533169483807022	26.730431812702584	23.62694543408963
40-41	24.050387077763787	25.406130293857654	26.681243470574056	23.862239157804506
42-43	23.809903297527523	25.465096032787766	26.43309180038038	24.291908869304333
44-45	23.626978918325253	25.540536015643834	26.724973882296215	24.107511183734697
46-47	23.614288393024562	25.51465270150813	26.20442795531864	24.666630950148672
48-49	23.750636200476816	25.937859955532932	25.94988079612119	24.361623047869063
50-51	23.943070102595698	25.956209316653716	26.02893707642442	24.071783504326163
52-53	24.068937344298305	25.786846052878303	25.920749511130158	24.223467091693234
54-55	23.492238354182852	25.43904529747395	26.430647451179983	24.638068897163215
56-57	23.674493048672684	25.701561704749405	25.817818970828533	24.806126275749378
58-59	23.838230960863626	25.77743698266856	26.111073906404865	24.27325815006295
60-61	24.36446920789692	25.62267284562428	26.286464332592214	23.726393613886582
62-63	23.99975891350352	25.556574964506712	26.307425464091505	24.136240657898263
64-65	23.670977203932388	25.190424847981568	26.38467359567116	24.753924352414884
66-67	23.692474083201628	25.58878679917495	26.114355361495807	24.604383756127614
68-69	23.498365969301652	25.58165465698749	26.209584527604402	24.710394846106453
70-71	23.619679354959686	25.48632503817203	26.253850687096513	24.64014491977177
72-73	23.65440250729956	26.044607698695454	25.760125632852056	24.54086416115293
74-75	23.517050172778657	25.929087085800006	25.96387720661113	24.58998553481021
76-77	23.429924191690553	25.295397926656133	26.074308215692056	25.200369665961265
78-79	23.446431919852134	25.45793040636468	26.175932870804424	24.919704802978757
80-81	23.74909592563821	25.731797969515952	25.762804371701804	24.75630173314404
82-83	23.72311215879564	25.722991615547404	25.9863451287134	24.56755109694356
84-85	23.613920066432723	25.8179194235354	25.65327743698267	24.914883073049207
86-87	23.135966087166164	26.18520800407168	25.768094880930057	24.910731027832096
88-89	23.08597412338271	25.72958800996491	25.99615600975061	25.188281856901774
90-91	23.56583286232049	25.356407158680877	26.012532513279886	25.06522746571875
92-93	23.67467099910061	25.46779370667538	25.94082772079106	24.916707573432948
94-95	23.59103264782128	25.566235545682908	25.704157204573413	25.138574601922397
96-97	23.165708231736108	25.420446775553447	25.907276359416116	25.506568633294336
98-99	22.90921851451157	25.498488353676684	25.75008898438596	25.84220414742578
100-101	23.090561463662908	25.751721089710966	25.866471565187112	25.291245881439018
102-103	22.982306929897405	25.608040234657526	25.868380166617555	25.541272668827514
104-105	23.238301692394288	25.565021552135477	25.648899590454178	25.54777716501606
106-107	23.218896378575828	25.37843084008348	25.795276508072888	25.607396273267803
108-109	23.12828042518496	25.334366885264014	25.848419452429543	25.688933237121482
110-111	22.893948901477533	25.91045737671214	25.80534986837285	25.39024385343747
112-113	22.871052339029724	25.97480220676576	25.729594850344156	25.424550603860368
114-115	22.885799699444696	25.971480730440465	25.602048169211027	25.540671400903808
116-117	22.62913722122048	25.686608370124446	25.75689236904624	25.92736203960883
118-119	22.65675618933262	25.327435175118175	26.026253746481213	25.989554889067996
120-121	22.706412034510215	25.41619258537984	26.09321079880716	25.784184581302792
122-123	22.64162189203325	25.644496888935915	25.742205262590417	25.971675956440414
124-125	22.31176656918283	25.466653362254803	25.680115507296552	26.54146456126581
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	119.0
1	97.0
2	89.0
3	94.5
4	78.0
5	97.5
6	222.5
7	627.5
8	920.5
9	1041.0
10	1030.0
11	890.5
12	907.0
13	950.5
14	1057.0
15	1174.5
16	1286.0
17	1354.5
18	1453.0
19	1523.5
20	1557.5
21	1667.0
22	1854.5
23	2076.5
24	2234.5
25	2537.5
26	3168.0
27	3955.0
28	4684.5
29	5618.0
30	7083.0
31	8737.0
32	11013.0
33	13662.5
34	16156.5
35	18868.0
36	22138.5
37	25448.5
38	27868.0
39	30494.5
40	33441.5
41	36124.5
42	38695.0
43	42235.0
44	46214.5
45	49848.0
46	53952.0
47	58494.0
48	63344.5
49	66859.5
50	68636.5
51	69696.0
52	69514.0
53	68426.5
54	66961.5
55	64190.0
56	59692.0
57	53977.5
58	48482.0
59	42395.5
60	35659.0
61	29916.0
62	24566.5
63	18771.5
64	13746.0
65	10386.5
66	7768.0
67	5805.5
68	4405.5
69	3328.0
70	2541.5
71	1896.0
72	1434.5
73	1199.0
74	940.0
75	705.5
76	537.0
77	396.5
78	294.0
79	212.0
80	190.0
81	162.0
82	126.5
83	127.0
84	124.5
85	118.0
86	117.0
87	107.0
88	92.0
89	86.0
90	80.0
91	70.5
92	66.0
93	66.5
94	62.0
95	56.5
96	50.0
97	37.5
98	24.0
99	10.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3509817577884332
2	0.0
3	0.0
4	0.020224478315609012
5	0.1846320752189869
6	0.052168439098872255
7	0.026184672256301732
8	0.00823712196297983
9	0.00689775253810506
10-11	0.0019755699016902844
12-13	0.054110524764940666
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	2.678738849749538E-4
92-93	4.687792987061691E-4
94-95	6.696847124373845E-5
96-97	4.687792987061691E-4
98-99	3.3484235621869224E-5
100-101	0.0
102-103	0.0
104-105	3.3484235621869224E-5
106-107	1.0045270686560768E-4
108-109	5.022635343280384E-4
110-111	4.687792987061691E-4
112-113	9.375585974123383E-4
114-115	2.678738849749538E-4
116-117	8.371058905467306E-4
118-119	3.6832659184056146E-4
120-121	6.696847124373845E-5
122-123	0.0014063378961185074
124-125	6.696847124373845E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1493240.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.0212191172776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.65325734511153	54.39236775710508
2	8.85320322058778	11.512921329912514
3	2.8159692365803464	5.492932582776108
4	1.417184936690443	3.6858836919301785
5	0.8053236295906522	2.618156208996754
6	0.5137560554294407	2.004302703174353
7	0.37347137859788054	1.699849503929117
8	0.2551621718587546	1.3272764389494822
9	0.20244868539312735	1.1847114299656194
>10	1.0499395183020657	11.85811362384685
>50	0.04576098720759668	1.9899290001036727
>100	0.01380188220009297	1.4430051127999701
>500	2.0598641438656075E-4	0.09589737254067811
>1k	5.149660359664019E-4	0.6946532439695907
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3313	0.22186654523050547	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2398	0.1605903940424848	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2389	0.15998767780129117	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	6.696847124373845E-5
9	0.0	0.0	0.0	6.696847124373845E-5	1.339369424874769E-4
10-11	0.0	0.0	0.0	1.339369424874769E-4	1.6742117810934613E-4
12-13	0.0	0.0	0.0	2.3438964935308456E-4	2.0090541373121533E-4
14-15	0.0	0.0	0.0	2.678738849749538E-4	2.3438964935308456E-4
16-17	0.0	0.0	0.0	2.678738849749538E-4	3.3484235621869226E-4
18-19	0.0	0.0	0.0	2.678738849749538E-4	4.0181082746243066E-4
20-21	0.0	0.0	0.0	2.678738849749538E-4	4.0181082746243066E-4
22-23	0.0	0.0	0.0	3.3484235621869226E-4	4.3529506308429987E-4
24-25	0.0	0.0	0.0	5.692320055717768E-4	5.357477699499076E-4
26-27	0.0	0.0	0.0	0.0012054324823872922	5.357477699499076E-4
28-29	0.0	0.0	0.0	0.002544801907262061	5.357477699499076E-4
30-31	0.0	0.0	0.0	0.010011786450938897	5.357477699499076E-4
32-33	0.0	0.0	0.0	0.026084219549436124	5.357477699499076E-4
34-35	0.0	0.0	0.0	0.04466797031957354	5.357477699499076E-4
36-37	0.0	6.696847124373845E-5	0.0	0.0746363612011465	5.357477699499076E-4
38-39	0.0	6.696847124373845E-5	0.0	0.12322198708847876	6.696847124373845E-4
40-41	0.0	6.696847124373845E-5	0.0	0.18101577777182504	6.696847124373845E-4
42-43	0.0	6.696847124373845E-5	0.0	0.23428919664621894	6.696847124373845E-4
44-45	0.0	6.696847124373845E-5	0.0	0.2935562936969275	6.696847124373845E-4
46-47	0.0	6.696847124373845E-5	0.0	0.3581138999758914	6.696847124373845E-4
48-49	0.0	6.696847124373845E-5	0.0	0.4256181189895797	7.366531836811229E-4
50-51	0.0	6.696847124373845E-5	0.0	0.49620288768048004	7.366531836811229E-4
52-53	0.0	6.696847124373845E-5	0.0	0.5731161769039137	8.036216549248613E-4
54-55	0.0	6.696847124373845E-5	0.0	0.6560566285392837	9.04074361790469E-4
56-57	0.0	6.696847124373845E-5	0.0	0.7414414293750502	9.375585974123383E-4
58-59	0.0	6.696847124373845E-5	0.0	0.8406217352870269	9.375585974123383E-4
60-61	0.0	6.696847124373845E-5	0.0	0.9487758163456644	9.375585974123383E-4
62-63	0.0	6.696847124373845E-5	0.0	1.063090996758726	9.375585974123383E-4
64-65	0.0	6.696847124373845E-5	0.0	1.1727853526559695	9.375585974123383E-4
66-67	0.0	6.696847124373845E-5	0.0	1.2977150357611635	9.375585974123383E-4
68-69	0.0	6.696847124373845E-5	0.0	1.433861937799684	9.375585974123383E-4
70-71	0.0	6.696847124373845E-5	0.0	1.580255015938496	9.375585974123383E-4
72-73	0.0	6.696847124373845E-5	0.0	1.7208888055503468	0.0011384640111435536
74-75	0.0	6.696847124373845E-5	0.0	1.8723045190324394	0.0011384640111435536
76-77	0.0	6.696847124373845E-5	0.0	2.0420361093996946	0.0011384640111435536
78-79	0.0	6.696847124373845E-5	0.0	2.240597626637379	0.0011384640111435536
80-81	0.0	6.696847124373845E-5	0.0	2.433065012991883	0.0011384640111435536
82-83	0.0	6.696847124373845E-5	0.0	2.6355776700329487	0.001205432482387292
84-85	0.0	6.696847124373845E-5	0.0	2.8461600278588843	0.001205432482387292
86-87	0.0	6.696847124373845E-5	0.0	3.076129758109882	0.001205432482387292
88-89	0.0	6.696847124373845E-5	0.0	3.3438027376711044	0.001205432482387292
90-91	0.0	6.696847124373845E-5	0.0	3.617301974230532	0.001205432482387292
92-93	0.0	6.696847124373845E-5	0.0	3.881090782459618	0.0012389167180091613
94-95	0.0	6.696847124373845E-5	0.0	4.15663255739198	0.001339369424874769
96-97	0.0	6.696847124373845E-5	0.0	4.467935495968498	0.001339369424874769
98-99	0.0	6.696847124373845E-5	0.0	4.822868393560311	0.001339369424874769
100-101	0.0	6.696847124373845E-5	0.0	5.202479172805443	0.001339369424874769
102-103	0.0	6.696847124373845E-5	0.0	5.599468270338324	0.0014398221317403767
104-105	0.0	6.696847124373845E-5	0.0	6.012261927084728	0.0014733063673622458
106-107	0.0	6.696847124373845E-5	0.0	6.459109051458573	0.0014733063673622458
108-109	0.0	6.696847124373845E-5	0.0	6.932877501272401	0.001506790602984115
110-111	0.0	6.696847124373845E-5	0.0	7.433600760761834	0.0015402748386059842
112-113	0.0	6.696847124373845E-5	0.0	7.932649808470172	0.0015402748386059842
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2535	0.0	71.197334	1
GTATCAA	4285	0.0	50.349037	1
GTACATG	8595	0.0	38.590633	1
TACATGG	8890	0.0	36.940506	2
ACATGGG	9110	0.0	35.982998	3
TATCAAC	6230	0.0	34.057674	2
CATGGGG	8185	0.0	33.641575	4
TCAACGC	6170	0.0	33.616085	4
CAACGCA	6285	0.0	33.09582	5
ATCAACG	6395	0.0	32.619743	3
ATGGGGG	5145	0.0	31.97251	5
AACGCAG	6500	0.0	31.712204	6
TGGTATC	1130	0.0	26.372093	2
TGGGGGG	4230	0.0	26.196075	6
ACGCAGA	7850	0.0	26.102352	7
CGCAGAG	8275	0.0	24.686455	8
GTGGTAT	1235	0.0	24.679596	1
CGTATAC	145	1.3250049E-4	24.662455	3
GTAAGTA	970	0.0	24.577702	2
AGAGTAC	8215	0.0	23.452263	10-11
>>END_MODULE