FastQCFastQC Report
Mon 15 Aug 2016
SRR2921293_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921293_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953523
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18200.060.166731
GTATCAA28250.048.6635131
GTACATG92750.037.9213261
TCAACGC36450.036.8957524
CAACGCA36900.036.6070675
TACATGG96450.036.1543732
ACATGGG99550.034.5503163
AACGCAG40400.033.434816
ATCAACG40750.032.856433
TATCAAC41850.032.135012
CATGGGG91650.031.6849334
ATGGGGG56200.029.965095
ACGCAGA50250.026.9931187
TGGGGGG39300.025.740136
CGCAGAG54400.025.1519768
ACCGTAT2201.5423757E-724.3367718
GAGTACA63800.022.0141181
GCAGAGT62200.021.1365419
GTGGTAT11700.020.8548931
AGTACAT66900.020.8139842
Produced by FastQC (version 0.11.5)