##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921293_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1953523
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.49247641312644	31.0	31.0	34.0	27.0	34.0
2	30.66386113703294	31.0	31.0	34.0	27.0	34.0
3	30.929707507922867	33.0	31.0	34.0	28.0	34.0
4	34.370275650709004	37.0	35.0	37.0	32.0	37.0
5	34.52038138276335	37.0	35.0	37.0	32.0	37.0
6	34.52827174289732	37.0	35.0	37.0	32.0	37.0
7	34.414411808819246	37.0	35.0	37.0	31.0	37.0
8	34.48638690202265	37.0	35.0	37.0	32.0	37.0
9	36.139154235706464	39.0	37.0	39.0	32.0	39.0
10-11	36.162766959999956	39.0	37.0	39.0	32.0	39.0
12-13	36.11323977245213	39.0	37.0	39.0	32.0	39.0
14-15	37.47924544528014	41.0	38.0	41.0	32.0	41.0
16-17	37.467271949191286	41.0	38.0	41.0	32.0	41.0
18-19	37.443908518097814	41.0	38.5	41.0	32.0	41.0
20-21	37.47791221296089	41.0	38.5	41.0	32.0	41.0
22-23	37.42075573207994	41.0	38.0	41.0	32.0	41.0
24-25	37.397072110233665	41.0	38.0	41.0	31.5	41.0
26-27	37.36182271721398	41.0	38.0	41.0	31.0	41.0
28-29	37.30013800707747	41.0	38.0	41.0	31.0	41.0
30-31	37.228037243482675	41.0	38.0	41.0	31.0	41.0
32-33	37.16006415076761	41.0	38.0	41.0	30.5	41.0
34-35	37.0663726508467	40.0	38.0	41.0	30.0	41.0
36-37	36.960248996300535	40.0	38.0	41.0	30.0	41.0
38-39	36.863168746925425	40.0	38.0	41.0	30.0	41.0
40-41	36.76631040433105	40.0	38.0	41.0	30.0	41.0
42-43	36.58244975871797	40.0	37.0	41.0	30.0	41.0
44-45	36.333056994977795	40.0	37.0	41.0	29.5	41.0
46-47	36.12729514830386	40.0	36.0	41.0	28.5	41.0
48-49	35.92911422082054	40.0	36.0	41.0	28.0	41.0
50-51	35.75716282838748	40.0	35.0	41.0	28.0	41.0
52-53	35.56198493695749	39.0	35.0	41.0	27.5	41.0
54-55	35.349710241445834	39.0	35.0	41.0	27.0	41.0
56-57	35.09802469691936	39.0	35.0	40.5	26.5	41.0
58-59	34.83061474065061	38.0	35.0	40.0	26.0	41.0
60-61	34.53519666776383	38.0	34.5	40.0	26.0	41.0
62-63	34.206709621540156	37.0	34.0	40.0	26.0	41.0
64-65	33.85171559280336	37.0	34.0	39.5	25.5	41.0
66-67	33.50277089135884	36.0	34.0	39.0	24.5	41.0
68-69	33.14005875538706	35.5	33.5	39.0	23.5	40.0
70-71	33.07661133244912	35.0	34.0	38.5	25.0	40.0
72-73	32.77819559841374	35.0	34.0	37.0	24.5	39.5
74-75	32.449886947837314	35.0	33.5	37.0	24.0	39.0
76-77	32.15138751885696	35.0	33.0	36.5	23.5	39.0
78-79	31.86744998651155	35.0	33.0	36.0	23.0	37.5
80-81	31.60451220692052	35.0	33.0	35.5	22.0	37.0
82-83	31.395655694865123	35.0	33.0	35.0	21.0	37.0
84-85	31.191127260851292	35.0	33.0	35.0	20.0	36.0
86-87	31.03098632573049	35.0	33.0	35.0	20.0	36.0
88-89	30.89857529192131	35.0	33.0	35.0	19.5	36.0
90-91	30.78389248552487	35.0	33.0	35.0	19.0	35.0
92-93	30.681705564766833	35.0	32.0	35.0	18.0	35.0
94-95	30.563720519287465	35.0	32.0	35.0	17.5	35.0
96-97	30.477139250472096	35.0	32.0	35.0	16.5	35.0
98-99	30.399703765965388	35.0	32.0	35.0	15.0	35.0
100-101	30.308012242497274	35.0	32.0	35.0	10.5	35.0
102-103	29.47524139720904	34.0	30.5	35.0	9.0	35.0
104-105	30.072663081008002	34.5	31.5	35.0	7.0	35.0
106-107	30.15262093151706	35.0	32.0	35.0	7.0	35.0
108-109	30.08591785200379	35.0	32.0	35.0	3.5	35.0
110-111	30.007045988196708	35.0	32.0	35.0	2.0	35.0
112-113	29.916422791029333	35.0	31.5	35.0	2.0	35.0
114-115	29.79350767817937	35.0	31.0	35.0	2.0	35.0
116-117	29.66872133064213	34.5	31.0	35.0	2.0	35.0
118-119	29.66241451981881	34.5	31.5	35.0	2.0	35.0
120-121	29.83177136895752	35.0	32.0	35.0	2.0	35.0
122-123	29.696389804471202	35.0	32.0	35.0	2.0	35.0
124-125	29.134438908576968	34.5	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	58544.0
3	8299.0
4	5325.0
5	4497.0
6	4649.0
7	5346.0
8	5774.0
9	6143.0
10	6601.0
11	7053.0
12	7146.0
13	7114.0
14	6993.0
15	6308.0
16	5156.0
17	4919.0
18	4678.0
19	4584.0
20	4675.0
21	4876.0
22	5589.0
23	7345.0
24	10155.0
25	12795.0
26	13947.0
27	16136.0
28	19356.0
29	23590.0
30	28876.0
31	36742.0
32	47529.0
33	64960.0
34	102496.0
35	175252.0
36	382844.0
37	661136.0
38	176024.0
39	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.13602460812543	17.411692545884243	12.378915161767296	25.07336768422303
2	16.75829042256146	18.735377145495928	39.143685640241685	25.362646791700925
3	20.2869858549804	22.89241562981405	28.04946789148642	28.77113062371913
4	14.104381598341625	14.58025830966749	34.2913839948762	37.023976097114684
5	15.058653923930617	36.95171610210708	32.73955855716569	15.250071416796615
6	34.96874302299621	33.3539699023578	16.689146700914154	14.98814037373185
7	30.99403138820142	31.02259962789612	19.248030685770868	18.735338298131595
8	27.841521423821035	34.984177433913075	18.42278121852958	18.751519923736307
9	27.854919903077885	15.962250956192046	18.122008620190947	38.060820520539124
10-11	27.135621151765882	25.017905311490985	26.080782007760078	21.765691528983055
12-13	26.74591979006446	22.25077129860499	26.512168894492905	24.491140016837644
14-15	24.282746155939183	23.655855353605475	23.60522491764875	28.45617357280659
16-17	24.870117786664256	26.7783503109571	24.185057421542748	24.166474480835895
18-19	24.871453056560654	26.24917322455733	25.45769571796872	23.421678000913293
20-21	25.6141892957262	25.155550254224966	25.21398718534157	24.016273264707266
22-23	25.581957282903307	24.630635053533954	25.11714911513927	24.67025854842347
24-25	25.004997764124475	24.931765481614242	25.180975374141195	24.882261380120084
26-27	24.704528153717543	25.164699925924065	25.33391761343421	24.79685430692418
28-29	24.895571793544047	25.628661770355983	25.091284292241816	24.384482143858158
30-31	25.32977984643592	25.231412216681132	25.093113933161714	24.34569400372123
32-33	25.01466720045379	25.132823914755154	25.071211433965857	24.7812974508252
34-35	25.009733341012495	25.390133553469386	25.183871194814007	24.416261910704115
36-37	25.01036678451525	25.396651093452594	25.152711694859203	24.440270427172955
38-39	25.040788963763223	25.443405600745795	25.109056404212872	24.406749031278107
40-41	24.939085339398137	25.26864779564312	25.18632757356282	24.605939291395924
42-43	24.834476922501974	25.20458411766844	25.098177339170135	24.86276162065945
44-45	24.67603740342555	25.231826034293388	25.291799909949596	24.800336652331467
46-47	24.73494153854345	25.128200008652048	25.214285293750997	24.922573159053506
48-49	24.593398852317097	25.371327995773363	25.246745680574733	24.788527471334802
50-51	24.602440224932558	25.39723981774207	25.354544712244458	24.645775245080912
52-53	24.875447171785638	25.282406772233678	25.200395415554922	24.641750640425762
54-55	24.512471504418254	25.0203110209878	25.45543017129857	25.011787303295375
56-57	24.697800903883998	25.242609099863415	25.164026072159185	24.895563924093402
58-59	24.686001615682564	25.377837299332327	25.318375471112763	24.617785613872346
60-61	24.979010869298122	25.218235766297802	25.37240349095317	24.4303498734509
62-63	24.848416901131575	25.358019723720478	25.30267986222883	24.49088351291912
64-65	24.721035206067285	25.085190005771906	25.28601650176421	24.907758286396604
66-67	24.7662051517798	25.273801564638877	25.186032511152224	24.773960772429103
68-69	24.51185568303345	25.263020210743807	25.352199561065586	24.872924545157158
70-71	24.544172630418217	25.1283852219606	25.506853824245283	24.8205883233759
72-73	24.639757797654152	25.496670434440734	25.057822021820325	24.80574974608479
74-75	24.5349105374205	25.46740591922503	25.23465961781281	24.76302392554166
76-77	24.527900555031685	25.115414031520395	25.296431558890593	25.06025385455733
78-79	24.43576459988533	25.174973380293224	25.415113850438203	24.97414816938324
80-81	24.64278437752439	25.416188450949655	25.092401002947618	24.848626168578335
82-83	24.713235487973847	25.36313180916136	25.229697011116837	24.693935691747953
84-85	24.721970938456554	25.50779372171138	24.987290758269452	24.782944581562617
86-87	24.371652398498483	25.61630423514385	25.219218704205453	24.792824662152217
88-89	24.395604170580786	25.273715481287212	25.324089266032455	25.006591082099543
90-91	24.65365374691818	25.156650585238395	25.29397581233055	24.895719855512873
92-93	24.653995513825205	25.380880745207747	25.296409805898577	24.668713935068467
94-95	24.65830601283125	25.248418619033092	25.177566198890528	24.915709169245133
96-97	24.464281417552044	25.20586839474103	25.31862565664147	25.01122453106546
98-99	24.362271441724598	25.265203494391837	25.218821839411454	25.153703224472107
100-101	24.350666720250363	25.36245146430673	25.32172614926148	24.965155666181428
102-103	24.31890753882678	25.259581112298505	25.44556571596777	24.97594563290694
104-105	24.405298363797808	25.32262411785017	25.302966040415264	24.96911147793675
106-107	24.41352601111681	25.20084112017959	25.39225611418347	24.993376754520124
108-109	24.38677583901413	25.183964688608985	25.41830401297454	25.010955459402346
110-111	24.257913608662886	25.54649872219128	25.323342944202352	24.87224472494348
112-113	24.21041611580709	25.594064461897325	25.359702240186465	24.83581718210912
114-115	24.296818749691255	25.703450004005713	25.217415341945564	24.78231590435747
116-117	24.171960322282906	25.6026935061704	25.291396352791157	24.933949818755536
118-119	24.262416369247365	25.40403478485574	25.359428298662472	24.974120547234424
120-121	24.128724552628753	25.49627637530164	25.548880429576737	24.826118642492865
122-123	24.126996477808866	25.705033321448507	25.318874629089805	24.849095571652825
124-125	24.133542770835632	25.620704315079728	25.24517062185994	25.0005822922247
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	201.0
1	186.0
2	166.0
3	136.5
4	105.0
5	100.0
6	101.5
7	130.5
8	171.5
9	201.0
10	224.5
11	212.5
12	220.5
13	247.5
14	272.5
15	302.5
16	339.5
17	373.0
18	396.0
19	446.5
20	522.0
21	584.0
22	645.0
23	796.0
24	935.5
25	1131.0
26	1483.0
27	1996.0
28	2762.5
29	3896.0
30	5511.5
31	7498.0
32	10114.5
33	13224.0
34	16307.0
35	19793.0
36	24217.5
37	28953.0
38	33224.5
39	37809.5
40	42635.0
41	46769.5
42	51060.5
43	56825.5
44	63150.5
45	69110.0
46	74074.0
47	78672.0
48	84901.0
49	90083.5
50	92004.5
51	92928.5
52	92887.0
53	91599.5
54	90545.0
55	88332.5
56	82997.5
57	75718.5
58	68591.0
59	61102.0
60	51973.5
61	43214.0
62	35710.5
63	27666.0
64	20445.0
65	15396.5
66	11778.5
67	9102.0
68	6957.5
69	5377.5
70	4191.0
71	3192.5
72	2362.5
73	1766.0
74	1347.5
75	978.0
76	730.5
77	489.0
78	283.5
79	200.5
80	151.5
81	97.0
82	64.5
83	50.0
84	39.0
85	30.0
86	24.5
87	17.5
88	12.5
89	13.0
90	13.0
91	5.0
92	3.5
93	3.5
94	2.0
95	2.0
96	2.0
97	1.5
98	0.5
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01776278037166698
2	0.01254144435463519
3	0.013104529611373912
4	0.014640216675206793
5	0.010289103327680299
6	0.03475771721141752
7	0.01561281848230095
8	0.015356870638328805
9	0.010340292896474727
10-11	0.009828397208530435
12-13	0.00826711536030034
14-15	0.007806409241150475
16-17	0.006065963902139877
18-19	0.0077296248879588315
20-21	0.006910591787247961
22-23	0.006987376140439606
24-25	0.007652840534767187
26-27	0.00624512739292038
28-29	0.007550461397178329
30-31	0.006987376140439606
32-33	0.009470070226969428
34-35	0.009495665011366644
36-37	0.00865103712625856
38-39	0.009009364107819565
40-41	0.00898376932342235
42-43	0.008702226695052988
44-45	0.008830200617039062
46-47	0.011645626900732677
48-49	0.010033155483708152
50-51	0.0070385657092340344
52-53	0.006910591787247961
54-55	0.007678435319164402
56-57	0.008958174539025136
58-59	0.00865103712625856
60-61	0.006705833512070245
62-63	0.007115350062425679
64-65	0.0048886038198680025
66-67	0.0051701464482373635
68-69	0.00798557273193098
70-71	0.006373101314906453
72-73	0.005886800411359375
74-75	0.0055540682141955846
76-77	0.0061939378241259516
78-79	0.0042487342099376355
80-81	0.004146355072348777
82-83	0.006884997002850748
84-85	0.011261705134774456
86-87	0.008471873635478057
88-89	0.0064498856680980975
90-91	0.006705833512070245
92-93	0.009649233717749933
94-95	0.008548657988669701
96-97	0.01085218858441902
98-99	0.008420684066683627
100-101	0.00985399199292765
102-103	0.006936186571645176
104-105	0.006526670021289741
106-107	0.00898376932342235
108-109	0.008139141438314266
110-111	0.009162932814202853
112-113	0.006987376140439606
114-115	0.003148158480857405
116-117	0.004299923778732065
118-119	0.1604281086017416
120-121	0.012874176551798979
122-123	0.0026362627929131114
124-125	0.0018684192609966712
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1953523.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.95024234562765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.1293425335267	49.64032188947788
2	10.847954559342167	13.440668278112073
3	3.5080429484999143	6.519723324553195
4	1.7063298406543654	4.228301886004565
5	0.9925400742152417	3.0744049067690735
6	0.6116288930283237	2.2734334889215573
7	0.4208190493840204	1.8248889465097702
8	0.3127507352444607	1.5499987073734056
9	0.22847665657009242	1.273876582035237
>10	1.1772833891011334	12.618263723204903
>50	0.051766731188788086	2.1518771031624744
>100	0.012899330858672502	1.3083130053507892
>500	8.262919304391596E-5	0.03680488045869962
>1k	8.262919304391596E-5	0.05912327806647851
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.118956879442935E-5	0.0
5	0.0	0.0	0.0	5.118956879442935E-5	0.0
6	0.0	0.0	0.0	5.118956879442935E-5	0.0
7	0.0	0.0	0.0	5.118956879442935E-5	0.0
8	0.0	0.0	0.0	5.118956879442935E-5	0.0
9	0.0	0.0	0.0	5.118956879442935E-5	0.0
10-11	0.0	0.0	0.0	5.118956879442935E-5	0.0
12-13	0.0	0.0	0.0	5.118956879442935E-5	5.118956879442935E-5
14-15	0.0	0.0	0.0	5.118956879442935E-5	5.118956879442935E-5
16-17	0.0	0.0	0.0	5.118956879442935E-5	5.118956879442935E-5
18-19	0.0	0.0	0.0	7.678435319164402E-5	5.118956879442935E-5
20-21	0.0	0.0	0.0	1.023791375888587E-4	7.678435319164402E-5
22-23	0.0	0.0	0.0	2.047582751777174E-4	1.7916349078050272E-4
24-25	0.0	0.0	0.0	3.071374127665761E-4	2.5594784397214675E-4
26-27	0.0	0.0	0.0	4.863009035470788E-4	2.5594784397214675E-4
28-29	0.0	0.0	0.0	0.0021243671049688178	2.5594784397214675E-4
30-31	0.0	0.0	0.0	0.009649233717749933	2.815426283693614E-4
32-33	0.0	0.0	0.0	0.024289450392956727	3.839217659582201E-4
34-35	2.5594784397214675E-5	0.0	0.0	0.04177068813625434	4.3511133475264944E-4
36-37	5.118956879442935E-5	0.0	0.0	0.06826128998737153	4.863009035470788E-4
38-39	5.118956879442935E-5	0.0	0.0	0.11187480260022534	5.118956879442935E-4
40-41	1.023791375888587E-4	0.0	0.0	0.16669883077905917	5.630852567387228E-4
42-43	1.023791375888587E-4	0.0	0.0	0.21632711772525842	5.630852567387228E-4
44-45	1.2797392198607337E-4	0.0	0.0	0.27652605062750735	5.630852567387228E-4
46-47	1.5356870638328805E-4	0.0	0.0	0.3402058742077775	5.630852567387228E-4
48-49	1.5356870638328805E-4	0.0	0.0	0.40790407893841024	6.142748255331522E-4
50-51	1.5356870638328805E-4	0.0	0.0	0.4797230439569946	6.654643943275815E-4
52-53	1.5356870638328805E-4	0.0	0.0	0.5576079728777189	7.422487475192256E-4
54-55	1.5356870638328805E-4	0.0	0.0	0.639767230792778	8.190331007108696E-4
56-57	1.5356870638328805E-4	0.0	0.0	0.7260472489957887	8.190331007108696E-4
58-59	1.5356870638328805E-4	0.0	0.0	0.8283751970158528	9.214122382997282E-4
60-61	1.5356870638328805E-4	0.0	0.0	0.9315477729210253	9.726018070941576E-4
62-63	1.5356870638328805E-4	0.0	0.0	1.0482599897723242	9.726018070941576E-4
64-65	1.5356870638328805E-4	0.0	0.0	1.1585735105243193	9.726018070941576E-4
66-67	1.5356870638328805E-4	0.0	0.0	1.2857539941940792	9.726018070941576E-4
68-69	1.5356870638328805E-4	0.0	0.0	1.425859843984432	9.726018070941576E-4
70-71	1.5356870638328805E-4	0.0	0.0	1.5730042594840192	0.001023791375888587
72-73	1.5356870638328805E-4	0.0	0.0	1.7096548133807485	0.0012285496510663044
74-75	1.5356870638328805E-4	0.0	0.0	1.84766189085053	0.0012285496510663044
76-77	1.5356870638328805E-4	0.0	0.0	2.0209385812196734	0.0012285496510663044
78-79	1.5356870638328805E-4	0.0	0.0	2.211901267607292	0.0012285496510663044
80-81	1.5356870638328805E-4	0.0	0.0	2.400893155596325	0.0012797392198607336
82-83	1.5356870638328805E-4	0.0	0.0	2.598433701574028	0.001407713141846807
84-85	1.5356870638328805E-4	0.0	0.0	2.8118942034467986	0.0014333079262440218
86-87	1.5356870638328805E-4	0.0	0.0	3.042093694315347	0.0015356870638328804
88-89	1.5356870638328805E-4	0.0	0.0	3.303416443010909	0.0016380662014217392
90-91	1.5356870638328805E-4	0.0	0.0	3.5792770292440887	0.0016380662014217392
92-93	1.5356870638328805E-4	0.0	0.0	3.849481168125484	0.0016636609858189538
94-95	1.5356870638328805E-4	0.0	0.0	4.1272357684040575	0.0017404453390105978
96-97	1.5356870638328805E-4	0.0	0.0	4.438084424908229	0.0017404453390105978
98-99	1.5356870638328805E-4	0.0	0.0	4.784023530820983	0.0017404453390105978
100-101	1.5356870638328805E-4	0.0	0.0	5.152230099159313	0.0017404453390105978
102-103	1.5356870638328805E-4	0.0	0.0	5.529906737724614	0.0017404453390105978
104-105	1.5356870638328805E-4	0.0	0.0	5.921609318139587	0.0017404453390105978
106-107	1.5356870638328805E-4	2.5594784397214675E-5	0.0	6.344076829400012	0.0017404453390105978
108-109	1.5356870638328805E-4	5.118956879442935E-5	0.0	6.790705817131409	0.0017404453390105978
110-111	1.5356870638328805E-4	5.118956879442935E-5	0.0	7.275650197105435	0.0018428244765994564
112-113	1.5356870638328805E-4	5.118956879442935E-5	0.0	7.758879726524848	0.0018940140453938858
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1820	0.0	60.16673	1
GTATCAA	2825	0.0	48.663513	1
GTACATG	9275	0.0	37.921326	1
TCAACGC	3645	0.0	36.895752	4
CAACGCA	3690	0.0	36.607067	5
TACATGG	9645	0.0	36.154373	2
ACATGGG	9955	0.0	34.550316	3
AACGCAG	4040	0.0	33.43481	6
ATCAACG	4075	0.0	32.85643	3
TATCAAC	4185	0.0	32.13501	2
CATGGGG	9165	0.0	31.684933	4
ATGGGGG	5620	0.0	29.96509	5
ACGCAGA	5025	0.0	26.993118	7
TGGGGGG	3930	0.0	25.74013	6
CGCAGAG	5440	0.0	25.151976	8
ACCGTAT	220	1.5423757E-7	24.336771	8
GAGTACA	6380	0.0	22.014118	1
GCAGAGT	6220	0.0	21.136541	9
GTGGTAT	1170	0.0	20.854893	1
AGTACAT	6690	0.0	20.813984	2
>>END_MODULE