FastQCFastQC Report
Mon 15 Aug 2016
SRR2921293_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921293_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953523
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20950.061.0647851
GTATCAA34800.049.3019071
GTACATG90950.036.8083531
TCAACGC47550.034.7222334
TACATGG96000.034.7071532
ACATGGG97400.033.96353
CAACGCA48850.033.5541735
TATCAAC49950.033.531212
ATCAACG50550.032.4257433
CATGGGG89100.032.243914
AACGCAG51800.031.3986436
ATGGGGG52500.030.1995685
ACGCAGA64950.024.6664377
CGCAGAG67150.023.8552478
GTGGTAT11500.022.872621
TGGGGGG38800.022.7252126
AGAGTAC69300.021.18009810-11
GCAGAGT76250.020.3829579
GGGTTAG5600.020.2827421
CAGAGTA73450.019.45645510-11
Produced by FastQC (version 0.11.5)