FastQCFastQC Report
Mon 15 Aug 2016
SRR2921292_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921292_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803465
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25250.056.7989121
GGTATCA14150.055.0933571
TCAACGC30300.045.7548834
CAACGCA31450.044.2697755
GTACATG86650.041.2066271
AACGCAG34050.041.063016
TATCAAC34500.040.357192
ATCAACG34450.040.2430463
TACATGG89350.039.6229252
TAGTACG1850.038.5952342
ACATGGG92350.038.0780533
CATGGGG83650.034.7834
ATGGGGG49950.032.16185
ACGCAGA43900.031.7113887
CGCAGAG48300.028.575438
AGTACAT57800.026.9710332
GAGTACA59800.026.6696931
TACGGTC1451.3405901E-424.6204155
GCAGAGT55450.024.2456979
TGGGGGG36000.023.6338026
Produced by FastQC (version 0.11.5)