##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921292_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1803465
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.449854030990345	31.0	31.0	34.0	27.0	34.0
2	30.551665821072213	33.0	31.0	34.0	27.0	34.0
3	30.574064370531172	33.0	31.0	34.0	27.0	34.0
4	34.04016934068585	37.0	35.0	37.0	30.0	37.0
5	34.1140587702007	37.0	35.0	37.0	30.0	37.0
6	34.046354101687584	37.0	35.0	37.0	30.0	37.0
7	34.08702026377002	37.0	35.0	37.0	30.0	37.0
8	34.08984704443945	37.0	35.0	37.0	30.0	37.0
9	35.68272353497296	39.0	37.0	39.0	30.0	39.0
10-11	35.71589301705328	39.0	37.0	39.0	30.0	39.0
12-13	35.651388299745214	39.0	37.0	39.0	30.0	39.0
14-15	36.97586174392073	41.0	38.0	41.0	30.0	41.0
16-17	36.94894550213062	41.0	38.0	41.0	30.0	41.0
18-19	36.92208997679467	41.0	38.0	41.0	30.0	41.0
20-21	36.94650076380745	41.0	38.0	41.0	30.0	41.0
22-23	36.880225011297696	40.0	38.0	41.0	30.0	41.0
24-25	36.852713526461564	40.5	38.0	41.0	30.0	41.0
26-27	36.8145830387615	40.0	38.0	41.0	30.0	41.0
28-29	36.74857205435093	40.0	38.0	41.0	30.0	41.0
30-31	36.671870538103036	40.0	38.0	41.0	30.0	41.0
32-33	36.594080007097446	40.0	38.0	41.0	30.0	41.0
34-35	36.47674338010441	40.0	37.5	41.0	29.5	41.0
36-37	36.360533750308434	40.0	37.0	41.0	29.0	41.0
38-39	36.25720127643176	40.0	37.0	41.0	28.0	41.0
40-41	36.15142683667274	40.0	37.0	41.0	28.0	41.0
42-43	35.94046959602765	40.0	36.5	41.0	27.0	41.0
44-45	35.657883851363906	40.0	36.0	41.0	26.5	41.0
46-47	35.42765565175925	40.0	35.0	41.0	26.0	41.0
48-49	35.21030211287716	39.5	35.0	41.0	25.0	41.0
50-51	35.02185321034786	39.0	35.0	41.0	24.0	41.0
52-53	34.8159462479172	39.0	35.0	41.0	24.0	41.0
54-55	34.59492088840094	39.0	35.0	40.5	23.5	41.0
56-57	34.33795804188049	38.0	34.0	40.0	23.0	41.0
58-59	34.056921814396176	38.0	34.0	40.0	22.5	41.0
60-61	33.754667820002055	37.0	34.0	40.0	22.0	41.0
62-63	33.425509505313386	37.0	33.5	40.0	21.5	41.0
64-65	33.071172437502256	36.0	33.0	39.0	20.0	41.0
66-67	32.73199174921609	36.0	33.0	39.0	19.5	40.5
68-69	32.38095832189701	35.0	33.0	38.5	18.5	40.0
70-71	32.33687124507545	35.0	33.0	37.5	19.5	40.0
72-73	32.057606330036904	35.0	33.0	37.0	18.5	39.0
74-75	31.738986617428118	35.0	33.0	37.0	17.5	39.0
76-77	31.45394088601664	35.0	33.0	36.0	16.0	39.0
78-79	31.178356108934747	35.0	32.5	36.0	13.0	37.0
80-81	30.916124793106604	35.0	32.0	35.0	10.5	37.0
82-83	30.702401765490322	35.0	32.0	35.0	7.5	36.5
84-85	30.50121017042194	35.0	32.0	35.0	7.0	36.0
86-87	30.34026083123321	35.0	32.0	35.0	6.0	36.0
88-89	30.20481933389337	35.0	32.0	35.0	2.0	35.5
90-91	30.083480134075238	35.0	32.0	35.0	2.0	35.0
92-93	29.97647001743865	35.0	31.0	35.0	2.0	35.0
94-95	29.849830742487377	35.0	31.0	35.0	2.0	35.0
96-97	29.759175254302136	35.0	31.0	35.0	2.0	35.0
98-99	29.674400944847832	35.0	31.0	35.0	2.0	35.0
100-101	29.576226597133854	34.0	31.0	35.0	2.0	35.0
102-103	28.698552231399002	33.5	29.5	34.5	2.0	35.0
104-105	29.3160615814557	34.0	30.5	35.0	2.0	35.0
106-107	29.399887161658253	34.0	31.0	35.0	2.0	35.0
108-109	29.340199005802717	34.0	31.0	35.0	2.0	35.0
110-111	29.25663237157361	34.0	31.0	35.0	2.0	35.0
112-113	29.159855334037534	34.0	31.0	35.0	2.0	35.0
114-115	29.024317910245003	34.0	30.0	35.0	2.0	35.0
116-117	28.882066743740523	34.0	30.0	35.0	2.0	35.0
118-119	28.87931703692614	34.0	30.5	35.0	2.0	35.0
120-121	29.070359557851134	35.0	31.0	35.0	2.0	35.0
122-123	28.935325054825018	35.0	31.0	35.0	2.0	35.0
124-125	28.331961529611053	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	68263.0
3	9402.0
4	5876.0
5	5162.0
6	5330.0
7	6073.0
8	6050.0
9	6442.0
10	7001.0
11	7509.0
12	7278.0
13	7139.0
14	7236.0
15	6410.0
16	5721.0
17	5332.0
18	4934.0
19	4936.0
20	4902.0
21	5200.0
22	5909.0
23	7489.0
24	9993.0
25	12569.0
26	13929.0
27	16307.0
28	19735.0
29	23477.0
30	28810.0
31	36129.0
32	47616.0
33	65871.0
34	103008.0
35	174593.0
36	362615.0
37	556637.0
38	132546.0
39	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.8745594551802	16.985189929928765	11.346105320367244	25.794145294523794
2	17.144590520121714	19.0037327027685	37.781318296787305	26.070358480322486
3	20.36684793157259	22.883187837098934	27.827860079611632	28.922104151716844
4	14.152824215052165	14.02904488608919	33.92562925318141	37.892501645677235
5	14.857200212500013	37.15949811844325	32.87256542748992	15.110736241566821
6	35.402844075065474	33.4772861147503	16.175219340331665	14.944650469852562
7	31.12661778423037	31.1194085306543	18.7515458303341	19.00242785478123
8	28.99200440096227	33.865338096606	18.430637706974437	18.712019795457294
9	28.189625485839198	15.28311899753954	17.654796173508572	38.87245934311269
10-11	27.356463658254924	24.80108354948408	25.872377944721787	21.97007484753921
12-13	26.769419733524092	22.068563966279473	26.392538341782874	24.769477958413567
14-15	24.21098890130395	23.60204395671425	23.33471038863458	28.852256753347216
16-17	24.964490477514705	26.70881692501814	24.012671694051672	24.314020903415486
18-19	24.948901053808328	26.119934744178103	25.25591062037392	23.675253581639648
20-21	25.512574072980442	25.01298261286417	25.270194998429336	24.204248315726055
22-23	25.545422685946107	24.570138607009536	25.049851056798044	24.834587650246316
24-25	25.139100992255592	24.880251707085527	25.077521701085637	24.903125599573244
26-27	24.739531781032024	25.167642885304154	25.257640207010475	24.835185126653347
28-29	24.853371336362855	25.513637006374196	25.113468432624565	24.519523224638384
30-31	25.326651779748282	25.10700781258231	25.0676648702182	24.49867553745121
32-33	24.909500957670623	25.175508253046857	25.0621349123346	24.852855876947917
34-35	25.04022405136257	25.321036866940805	25.224022249868504	24.414716831828116
36-37	24.949822427260887	25.323156322512798	25.1815861131436	24.545435137082716
38-39	25.021564123287725	25.493299248478866	25.084419626332767	24.400717001900638
40-41	25.047723905871404	25.290190460320467	25.067160047057087	24.59492558675104
42-43	24.816376007814362	25.213083127997383	25.027899534504066	24.94264132968419
44-45	24.647176399184957	25.23031511727238	25.293059645564114	24.829448837978546
46-47	24.692968227957955	25.131244402669527	25.234387780282425	24.941399589090093
48-49	24.618843181987064	25.396129152466056	25.196692112241493	24.788335553305394
50-51	24.684750027795175	25.39206447039031	25.269627067760066	24.653558434054453
52-53	24.858750953074097	25.246052540375686	25.17890067235045	24.716295834199762
54-55	24.473705223909565	25.04888092351952	25.4363239327434	25.04108991982752
56-57	24.572350034158763	25.280146217249733	25.08908450079408	25.058419247797424
58-59	24.65037975167875	25.350465896932345	25.255780978673297	24.743373372715606
60-61	24.929950942092592	25.262049741069404	25.31373050257322	24.494268814264778
62-63	24.78656626205382	25.344050305816335	25.20575367229132	24.663629759838525
64-65	24.628251319381004	25.05752320959963	25.29244026222654	25.021785208792824
66-67	24.700135355664894	25.244469187871555	25.148954999925145	24.906440456538412
68-69	24.49528906889865	25.23940047433706	25.243836640402716	25.021473816361578
70-71	24.47180594498514	25.043674799425858	25.44633413099972	25.03818512458928
72-73	24.618171665616334	25.433134329103073	25.044672674407803	24.904021330872787
74-75	24.44547706356393	25.434776701968566	25.239894488013455	24.879851746454055
76-77	24.50487637713016	25.026145233305314	25.270518601654217	25.19845978791031
78-79	24.397238784989984	25.086710779418315	25.399285464362215	25.11676497122949
80-81	24.605365558959296	25.346960853871515	25.05678826689113	24.99088532027806
82-83	24.68462648558053	25.263457041024413	25.292375001906148	24.759541471488912
84-85	24.685710324749593	25.35356462066336	24.993505008144734	24.96722004644231
86-87	24.32963650982893	25.542462638977458	25.16067319156015	24.96722765963346
88-89	24.34989562674828	25.183911242395197	25.234482848490075	25.231710282366453
90-91	24.584252902882366	25.021792412026304	25.340638135057503	25.053316550033827
92-93	24.70547624262824	25.142278057044308	25.266908256092186	24.885337444235265
94-95	24.671874384825124	25.007305851501844	25.269900612693384	25.05091915097965
96-97	24.36492600981471	24.978047355604	25.371850207964396	25.28517642661689
98-99	24.284131121487476	25.05744168978798	25.26036951773934	25.398057670985203
100-101	24.342605069510295	25.133835737219478	25.344751099355083	25.178808093915144
102-103	24.191750680530316	25.054883265968826	25.449199915935306	25.304166137565552
104-105	24.188732164750544	25.18139539010337	25.298481019817775	25.331391425328313
106-107	24.276121778342656	24.973632289382714	25.436106961408317	25.314138970866313
108-109	24.259753913959166	24.9802174061318	25.480535424844717	25.279493255064317
110-111	24.123403377514364	25.32950007153401	25.442457265360126	25.104639285591496
112-113	24.070562140431136	25.428710057531017	25.38257433977958	25.118153462258263
114-115	24.12135619103386	25.548479179957397	25.253539319908274	25.076625309100464
116-117	23.98407795006241	25.41372987078375	25.343113688449275	25.25907849070456
118-119	24.009801528489717	25.247183407380746	25.41063478059722	25.332380283532313
120-121	23.904999541112826	25.335174797195435	25.574295220003822	25.18553044168792
122-123	23.95898025090051	25.42732601983782	25.38651476302855	25.227178966233122
124-125	23.867375130480347	25.423685111585826	25.31267458027955	25.396265177654282
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	353.0
1	279.0
2	215.0
3	186.0
4	150.5
5	138.5
6	131.0
7	173.5
8	232.5
9	266.0
10	260.5
11	269.0
12	291.5
13	315.0
14	341.0
15	376.5
16	433.0
17	465.5
18	501.0
19	526.5
20	569.0
21	645.5
22	693.5
23	819.0
24	1013.5
25	1239.5
26	1658.0
27	2256.0
28	3035.5
29	4226.5
30	5999.0
31	7870.0
32	10441.5
33	13054.5
34	15593.0
35	18759.5
36	22221.0
37	25920.5
38	29446.5
39	33340.5
40	37145.5
41	40674.5
42	44473.0
43	49639.5
44	55262.5
45	60547.0
46	65596.5
47	70306.0
48	75910.5
49	80273.5
50	82311.0
51	84288.5
52	84958.5
53	84646.5
54	84455.5
55	82718.5
56	78143.5
57	71140.5
58	64592.0
59	58269.0
60	50489.5
61	42592.5
62	35566.0
63	27920.0
64	20721.0
65	15645.0
66	11746.0
67	8950.0
68	6979.5
69	5351.0
70	4241.0
71	3339.0
72	2528.0
73	2066.5
74	1639.5
75	1181.5
76	870.0
77	572.0
78	355.0
79	235.5
80	162.0
81	114.0
82	69.0
83	46.5
84	43.0
85	33.5
86	27.0
87	20.0
88	15.5
89	10.5
90	7.5
91	6.5
92	4.5
93	3.0
94	5.0
95	4.5
96	3.5
97	5.0
98	4.0
99	3.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016634644975089617
2	0.011866046748897262
3	0.011699700299146364
4	0.014194897045409808
5	0.009481747635801082
6	0.030995888470250324
7	0.012586881364484478
8	0.012365086098149949
9	0.007873731954875753
10-11	0.007346968197331248
12-13	0.006237991865658607
14-15	0.005544881658363206
16-17	0.004186385652064221
18-19	0.0056003304749468385
20-21	0.0050735667174023336
22-23	0.005156739942277782
24-25	0.005489432841779575
26-27	0.004325007693523301
28-29	0.005544881658363206
30-31	0.0051012911256941495
32-33	0.0072637949724558
34-35	0.007125172930996721
36-37	0.006626133581744031
38-39	0.006792480031494927
40-41	0.00684792884807856
42-43	0.006626133581744031
44-45	0.0066815823983276635
46-47	0.008317322487544809
48-49	0.007707385505124857
50-51	0.005239913167153229
52-53	0.004990393492526886
54-55	0.005711228108114102
56-57	0.00670930680661948
58-59	0.006404338315409504
60-61	0.004768598226192357
62-63	0.005267637575445046
64-65	0.0034655510364770036
66-67	0.00377051952768698
68-69	0.005877574557864999
70-71	0.004408180918398749
72-73	0.0038536927525624283
74-75	0.00377051952768698
76-77	0.004269558876939669
78-79	0.0031051337286833957
80-81	0.0030774093203915797
82-83	0.004851771451067806
84-85	0.00845594452900389
86-87	0.006376613907117687
88-89	0.0045190785515660135
90-91	0.004713149409608725
92-93	0.0072083461558721685
94-95	0.006432062723701319
96-97	0.008400495712420257
98-99	0.006348889498825871
100-101	0.007485590238790328
102-103	0.0051012911256941495
104-105	0.004657700593025093
106-107	0.006820204439786744
108-109	0.006210267457366791
110-111	0.007041999706121272
112-113	0.004990393492526886
114-115	0.002578369971138891
116-117	0.0029942360955161314
118-119	0.16243730818175012
120-121	0.010507550742598275
122-123	0.0022734014799289147
124-125	0.0018020865389680421
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1803465.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.06163597485074
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.76688697386194	50.12525138193733
2	10.393509443158184	12.900763991249134
3	3.3989997389430133	6.32842453430682
4	1.6306940157434804	4.048141535657575
5	0.9163130542758444	2.8433943606735554
6	0.6192376791436249	2.3058542054953843
7	0.4184185803710246	1.8177419134070247
8	0.30509843267097875	1.5147926291939031
9	0.23408692214064125	1.3075035613529133
>10	1.256944735660616	13.668381426491205
>50	0.0490024057920837	2.036722965328917
>100	0.010540150529856126	0.9963548504225958
>500	2.678677087716007E-4	0.10667264448353592
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	5.544881658363206E-5
5	0.0	0.0	0.0	0.0	5.544881658363206E-5
6	0.0	0.0	0.0	0.0	5.544881658363206E-5
7	0.0	0.0	0.0	0.0	5.544881658363206E-5
8	0.0	0.0	0.0	0.0	5.544881658363206E-5
9	0.0	0.0	0.0	0.0	1.1089763316726412E-4
10-11	0.0	0.0	0.0	0.0	1.1089763316726412E-4
12-13	0.0	0.0	0.0	0.0	2.2179526633452824E-4
14-15	0.0	0.0	0.0	0.0	3.049684912099763E-4
16-17	0.0	0.0	0.0	0.0	3.326928995017924E-4
18-19	0.0	0.0	0.0	0.0	3.8814171608542446E-4
20-21	0.0	5.544881658363206E-5	0.0	0.0	3.8814171608542446E-4
22-23	0.0	5.544881658363206E-5	0.0	1.663464497508962E-4	5.267637575445046E-4
24-25	0.0	5.544881658363206E-5	0.0	3.326928995017924E-4	7.208346155872168E-4
26-27	0.0	5.544881658363206E-5	0.0	7.762834321708488E-4	7.208346155872168E-4
28-29	0.0	5.544881658363206E-5	0.0	0.004990393492526886	7.485590238790329E-4
30-31	0.0	5.544881658363206E-5	0.0	0.017715896898470446	7.762834321708489E-4
32-33	0.0	5.544881658363206E-5	0.0	0.03950728181583785	7.762834321708489E-4
34-35	0.0	5.544881658363206E-5	0.0	0.06728713892423752	7.762834321708489E-4
36-37	0.0	5.544881658363206E-5	0.0	0.1096500347941324	7.762834321708489E-4
38-39	0.0	5.544881658363206E-5	0.0	0.17893333111538068	7.762834321708489E-4
40-41	0.0	5.544881658363206E-5	0.0	0.25825286323826635	8.31732248754481E-4
42-43	0.0	5.544881658363206E-5	0.0	0.3336632537920059	8.31732248754481E-4
44-45	0.0	5.544881658363206E-5	0.0	0.4181395258571694	8.31732248754481E-4
46-47	0.0	5.544881658363206E-5	0.0	0.510295459019166	8.31732248754481E-4
48-49	0.0	5.544881658363206E-5	0.0	0.6049743133357176	8.31732248754481E-4
50-51	0.0	5.544881658363206E-5	0.0	0.7058357107013444	8.31732248754481E-4
52-53	0.0	5.544881658363206E-5	0.0	0.8064475883923448	0.0010535275150890093
54-55	0.0	8.31732248754481E-5	0.0	0.9212266387204631	0.0012198739648399055
56-57	0.0	1.1089763316726412E-4	0.0	1.0429367911215355	0.0012198739648399055
58-59	0.0	1.1089763316726412E-4	0.0	1.1757089824310425	0.0013030471897153536
60-61	0.0	1.1089763316726412E-4	0.0	1.3121407956350692	0.0013862204145908017
62-63	0.0	1.1089763316726412E-4	0.0	1.4534521046984556	0.0013862204145908017
64-65	0.0	1.1089763316726412E-4	0.0	1.5987002797392798	0.0013862204145908017
66-67	0.0	1.1089763316726412E-4	0.0	1.7646308633657986	0.0013862204145908017
68-69	0.0	1.3862204145908015E-4	0.0	1.9505230209624251	0.0013862204145908017
70-71	0.0	1.663464497508962E-4	0.0	2.1467009340353154	0.0013862204145908017
72-73	0.0	1.663464497508962E-4	0.0	2.334672422253828	0.0014416692311744336
74-75	0.0	1.663464497508962E-4	0.0	2.5291591464209175	0.0014416692311744336
76-77	0.0	1.663464497508962E-4	0.0	2.7512593812466557	0.0014693936394662497
78-79	0.0	1.663464497508962E-4	0.0	2.9975353001028573	0.0014971180477580657
80-81	0.0	1.663464497508962E-4	0.0	3.2504373525408035	0.0014971180477580657
82-83	0.0	1.663464497508962E-4	0.0	3.5129320502477173	0.0014971180477580657
84-85	0.0	1.663464497508962E-4	0.0	3.782801440560255	0.0014971180477580657
86-87	0.0	1.663464497508962E-4	0.0	4.074129522890658	0.0015525668643416976
88-89	0.0	1.663464497508962E-4	0.0	4.399198210112201	0.0016080156809253298
90-91	0.0	1.663464497508962E-4	0.0	4.751852683584101	0.0016080156809253298
92-93	0.0	1.663464497508962E-4	0.0	5.1075568419681	0.001663464497508962
94-95	0.0	1.663464497508962E-4	0.0	5.468362291477794	0.001774362130676226
96-97	0.0	1.663464497508962E-4	0.0	5.848796622058094	0.001829810947259858
98-99	0.0	1.663464497508962E-4	0.0	6.275613887710602	0.0018852597638434902
100-101	0.0	1.663464497508962E-4	0.0	6.744489080741794	0.0018852597638434902
102-103	0.0	1.663464497508962E-4	0.0	7.2275037220018135	0.0019407085804271221
104-105	0.0	1.663464497508962E-4	0.0	7.727929291669092	0.0019407085804271221
106-107	0.0	1.663464497508962E-4	0.0	8.246763868442137	0.0020238818053025702
108-109	0.0	1.663464497508962E-4	0.0	8.792629743299702	0.0021347794384698345
110-111	0.0	1.663464497508962E-4	0.0	9.382716049382715	0.0023842991130961786
112-113	0.0	1.663464497508962E-4	0.0	9.975602520703202	0.002467472337971627
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2525	0.0	56.798912	1
GGTATCA	1415	0.0	55.093357	1
TCAACGC	3030	0.0	45.754883	4
CAACGCA	3145	0.0	44.269775	5
GTACATG	8665	0.0	41.206627	1
AACGCAG	3405	0.0	41.06301	6
TATCAAC	3450	0.0	40.35719	2
ATCAACG	3445	0.0	40.243046	3
TACATGG	8935	0.0	39.622925	2
TAGTACG	185	0.0	38.595234	2
ACATGGG	9235	0.0	38.078053	3
CATGGGG	8365	0.0	34.783	4
ATGGGGG	4995	0.0	32.1618	5
ACGCAGA	4390	0.0	31.711388	7
CGCAGAG	4830	0.0	28.57543	8
AGTACAT	5780	0.0	26.971033	2
GAGTACA	5980	0.0	26.669693	1
TACGGTC	145	1.3405901E-4	24.620415	5
GCAGAGT	5545	0.0	24.245697	9
TGGGGGG	3600	0.0	23.633802	6
>>END_MODULE