FastQCFastQC Report
Mon 15 Aug 2016
SRR2921292_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921292_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803465
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20050.064.099541
GTATCAA31400.046.6409341
GTACATG83500.038.4431231
TACATGG85600.037.454022
ACATGGG88100.036.932333
TCAACGC41650.033.766574
CATGGGG78050.033.670974
CAACGCA43400.032.6796345
AACGCAG45400.031.23746
ATCAACG45450.030.9434053
TATCAAC46700.030.8807932
ATGGGGG44450.030.1648185
GTGGTAT10800.029.8882751
ACGCAGA58750.024.7245981
GTAAGTA7200.024.0024452
CGCAGAG64800.023.082812
AGAGTAC64200.022.9272465
TGGTATC12500.022.8834322
ATGGGGA30650.022.1648255
CAGAGTA67900.021.941194
Produced by FastQC (version 0.11.5)