##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921292_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1803465
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.330371257551437	33.0	30.0	34.0	19.0	34.0
2	30.083088388186074	33.0	31.0	34.0	26.0	34.0
3	30.32144399808147	33.0	31.0	34.0	27.0	34.0
4	32.55586274199943	37.0	35.0	37.0	19.0	37.0
5	32.24256029365693	37.0	33.0	37.0	19.0	37.0
6	32.37082560515452	37.0	35.0	37.0	17.0	37.0
7	32.581797262491925	37.0	35.0	37.0	17.0	37.0
8	32.818811011026	37.0	35.0	37.0	17.0	37.0
9	34.57059327461304	39.0	37.0	39.0	19.0	39.0
10-11	34.746248748936075	39.0	37.0	39.0	25.5	39.0
12-13	34.77023867943099	39.0	37.0	39.0	26.0	39.0
14-15	36.18404349405173	41.0	38.0	41.0	27.0	41.0
16-17	36.18954429390091	41.0	38.0	41.0	27.0	41.0
18-19	36.206793866251914	41.0	38.0	41.0	27.0	41.0
20-21	36.195752343405616	41.0	38.0	41.0	27.0	41.0
22-23	36.15027377853188	41.0	38.0	41.0	26.5	41.0
24-25	36.094574887785456	41.0	38.0	41.0	26.0	41.0
26-27	35.99531402051052	41.0	38.0	41.0	25.0	41.0
28-29	35.92217675419262	40.0	38.0	41.0	25.0	41.0
30-31	35.834912238385556	40.0	37.5	41.0	24.5	41.0
32-33	35.71519186676758	40.0	37.0	41.0	24.0	41.0
34-35	35.633683769854144	40.0	37.0	41.0	24.0	41.0
36-37	35.55211772892737	40.0	37.0	41.0	23.0	41.0
38-39	35.45402489097377	40.0	36.5	41.0	22.0	41.0
40-41	35.3241182945053	40.0	36.0	41.0	20.5	41.0
42-43	35.18609038711591	40.0	36.0	41.0	18.5	41.0
44-45	35.04286054899873	40.0	35.0	41.0	16.0	41.0
46-47	34.84253312373681	40.0	35.0	41.0	13.5	41.0
48-49	34.638594039806705	40.0	35.0	41.0	8.5	41.0
50-51	34.454260548444246	40.0	35.0	41.0	7.5	41.0
52-53	34.25778986561979	39.0	35.0	41.0	6.5	41.0
54-55	34.044873063796636	39.0	35.0	41.0	2.0	41.0
56-57	33.792674102352976	39.0	35.0	41.0	2.0	41.0
58-59	33.71962194996853	38.5	35.0	41.0	2.0	41.0
60-61	33.47196923699656	37.5	34.5	41.0	2.0	41.0
62-63	33.17388582534177	37.0	34.0	40.0	2.0	41.0
64-65	32.86630763557929	36.5	34.0	40.0	2.0	41.0
66-67	32.54197281344523	36.0	34.0	39.0	2.0	41.0
68-69	32.20442204312255	35.5	34.0	39.0	2.0	41.0
70-71	31.87810658926012	35.0	33.5	38.0	2.0	40.5
72-73	31.541667567709936	35.0	33.0	37.0	2.0	39.5
74-75	31.21407512760159	35.0	33.0	37.0	2.0	39.0
76-77	30.929010543592476	35.0	33.0	36.0	2.0	39.0
78-79	30.679743992813833	35.0	33.0	36.0	2.0	37.0
80-81	30.460128973947374	35.0	33.0	35.0	2.0	37.0
82-83	30.270730787678165	35.0	33.0	35.0	2.0	36.5
84-85	30.10869991932197	35.0	32.5	35.0	2.0	36.0
86-87	29.974489940198453	35.0	32.0	35.0	2.0	36.0
88-89	29.85589656577754	35.0	32.0	35.0	2.0	36.0
90-91	29.762674074628563	35.0	32.0	35.0	2.0	35.0
92-93	29.70072970642624	35.0	32.0	35.0	2.0	35.0
94-95	29.631988422287098	35.0	32.0	35.0	2.0	35.0
96-97	29.56889016421167	35.0	32.0	35.0	2.0	35.0
98-99	29.512981954182642	35.0	32.0	35.0	2.0	35.0
100-101	29.45680925329851	35.0	32.0	35.0	2.0	35.0
102-103	28.773115641279425	34.0	30.0	35.0	2.0	35.0
104-105	29.328094251898428	35.0	31.5	35.0	2.0	35.0
106-107	29.389071592739533	35.0	32.0	35.0	2.0	35.0
108-109	29.460140618198857	35.0	32.0	35.0	2.0	35.0
110-111	29.573845347705667	35.0	32.5	35.0	2.0	35.0
112-113	29.533725910954743	35.0	33.0	35.0	2.0	35.0
114-115	29.469156873019436	35.0	32.0	35.0	2.0	35.0
116-117	29.405420953553296	35.0	32.0	35.0	2.0	35.0
118-119	29.326335415436397	35.0	32.0	35.0	2.0	35.0
120-121	29.22758883593527	35.0	32.0	35.0	2.0	35.0
122-123	29.110798379785578	35.0	32.0	35.0	2.0	35.0
124-125	28.570129722506397	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	115021.0
3	7810.0
4	5838.0
5	5400.0
6	5485.0
7	5731.0
8	6042.0
9	5957.0
10	6214.0
11	6243.0
12	6786.0
13	7678.0
14	8652.0
15	7682.0
16	5366.0
17	4742.0
18	4361.0
19	3972.0
20	3847.0
21	4014.0
22	4576.0
23	5695.0
24	7424.0
25	9632.0
26	11649.0
27	13454.0
28	16149.0
29	19248.0
30	23305.0
31	28742.0
32	37135.0
33	50435.0
34	76301.0
35	132257.0
36	307157.0
37	628966.0
38	204250.0
39	249.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.13290687037173	17.28616198915146	11.037344444518638	27.543586695958172
2	16.532785499025486	19.300624076430648	36.29125045398718	27.875339970556677
3	19.668305179196715	22.092804684316025	28.051611758476046	30.187278378011218
4	13.69662679574434	15.199883315438742	30.83145514077571	40.27203474804121
5	14.59703835452537	37.24270017547241	32.335082873081625	15.825178596920594
6	35.67171167452764	32.87021781075284	15.696886785230454	15.761183729489053
7	31.293925814180497	30.984892182092466	18.17702324962286	19.54415875410418
8	28.970698081159902	33.74692027910603	17.790819090692825	19.491562549041245
9	27.831920508428432	15.402961470276889	17.46555459500344	39.29956342629124
10-11	27.48847266362635	24.942214296586425	25.097002629155934	22.472310410631295
12-13	26.82335820365554	22.179507949330233	26.007175923871074	24.98995792314316
14-15	24.393930572536757	23.415342133060523	23.118663239929802	29.072064054472918
16-17	24.809963043363748	26.49300097312673	23.753524465404098	24.943511518105424
18-19	24.767821942760186	25.688604990947983	25.40420801069053	24.1393650556013
20-21	25.392175617491887	24.66069482912061	25.48483059000313	24.462298963384374
22-23	25.27379239408583	24.19647733668244	25.313271951493377	25.216458317738354
24-25	24.902202149750618	24.6204112638726	25.27942044896907	25.19796613740771
26-27	24.55184880216694	24.789862847352182	25.51455115569196	25.14373719478892
28-29	24.680240536966338	25.078030347137314	25.379394665269356	24.86233445062699
30-31	25.128183801737215	24.880133520750334	25.263395740976396	24.728286936536055
32-33	24.72071817307239	24.978499721369694	25.30062962131231	25.000152484245604
34-35	24.907331165284603	25.08889831518771	25.436479221942204	24.56729129758548
36-37	24.888034977113502	24.979276004801868	25.53093628099242	24.60175273709221
38-39	24.860643261721187	25.159318312248917	25.463732315293058	24.51630611073683
40-41	24.846753333166987	25.19480555486244	25.295750125452948	24.66269098651762
42-43	24.67749582054545	25.100930708386354	25.200627680603727	25.020945790464467
44-45	24.513145528191565	25.12521729004999	25.42142486823975	24.940212313518696
46-47	24.51214744949306	25.092280692999307	25.20958266448198	25.185989193025648
48-49	24.588888611644805	25.34870374529032	25.07370533944379	24.988702303621086
50-51	24.56055426637057	25.428855009661955	25.14528976165326	24.865300962314212
52-53	24.76771104512702	25.284438566869888	25.113018550401588	24.834831837601506
54-55	24.36107714871095	25.058373741658418	25.36287091792743	25.21767819170319
56-57	24.404743091770563	25.24401637958042	25.078141244770485	25.273099283878537
58-59	24.471614364570424	25.28862495252195	25.21942482942558	25.020335853482045
60-61	24.837216136714602	25.138885423337854	25.258322174258996	24.765576265688548
62-63	24.54649799136662	25.205202207971876	25.29322720429839	24.955072596363113
64-65	24.43185756307996	24.904863692946634	25.309861849273478	25.353416894699926
66-67	24.478711813093128	25.203122877349994	25.110412456022157	25.207752853534725
68-69	24.261879215842836	25.168827784293015	25.283690007846012	25.28560299201814
70-71	24.23127146908867	25.020446751115216	25.370661476657435	25.37762030313868
72-73	24.368174597233658	25.32003670711658	25.030067120792477	25.28172157485729
74-75	24.128164394651407	25.3429925171822	25.2572963711522	25.271546717014193
76-77	24.233933012284687	24.9825474849803	25.17689558710593	25.60662391562908
78-79	24.113969497605996	25.068853567992726	25.32752229735537	25.48965463704591
80-81	24.26193466465942	25.282886000005544	25.05751428500137	25.39766505033366
82-83	24.30928795402184	25.232566198955897	25.150446501595543	25.30769934542672
84-85	24.287552017921058	25.242131119816573	24.94525815582781	25.525058706434557
86-87	23.922310663084673	25.44343804842345	25.127352069488456	25.506899219003422
88-89	23.88488271189072	25.16023321772255	25.18964881492017	25.76523525546656
90-91	24.070696308568998	25.022144926379035	25.245465592398613	25.66169317265335
92-93	24.109716042342047	25.057119513068105	25.241487799393997	25.59167664519585
94-95	24.076845491146756	25.01273937973776	25.15266462356034	25.757750505555144
96-97	23.83353063979972	24.905639453160738	25.323476327382714	25.937353579656826
98-99	23.707696018497725	25.002897200666496	25.209444042440527	26.079962738395256
100-101	23.790730621331715	25.1031486610497	25.269439661984016	25.83668105563457
102-103	23.568796732955725	24.972039934237703	25.37440427177683	26.08475906102974
104-105	23.58959003917459	25.104340810606253	25.18327220101305	26.122796949206112
106-107	23.647995812497243	24.910484643004576	25.274312759102425	26.167206785395752
108-109	23.58262335350757	24.885012379016942	25.346487713860338	26.185876553615145
110-111	23.45550623664023	25.239082760590087	25.28588182128193	26.019529181487755
112-113	23.38723651535571	25.334587966006257	25.24589682161879	26.03227869701924
114-115	23.363242524504297	25.336117982166495	25.184797658277784	26.115841835051423
116-117	23.12969793484939	25.20383131904814	25.316559575746954	26.349911170355515
118-119	23.193048245911953	25.093258314510702	25.33360207623297	26.380091363344377
120-121	23.063788094408345	25.138435016843978	25.442350233314954	26.35542665543273
122-123	23.03069302593545	25.225222477721733	25.253169084177507	26.49091541216531
124-125	22.81881501898985	25.174837195208	25.14251050825259	26.863837277549557
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	67.0
1	51.0
2	58.0
3	68.5
4	54.0
5	74.5
6	183.0
7	402.5
8	582.5
9	663.0
10	622.5
11	528.0
12	525.5
13	563.5
14	612.0
15	659.0
16	726.0
17	798.0
18	813.0
19	886.0
20	976.5
21	1044.0
22	1144.0
23	1252.5
24	1398.0
25	1601.5
26	1983.5
27	2575.0
28	3282.5
29	4265.5
30	5736.0
31	7452.0
32	9763.5
33	12067.5
34	14310.0
35	17163.5
36	20631.5
37	24269.5
38	27762.5
39	31498.5
40	35149.5
41	38708.0
42	42675.0
43	47928.0
44	53400.5
45	58744.5
46	64051.5
47	69104.0
48	75105.5
49	79972.0
50	82467.0
51	84956.5
52	86017.0
53	85673.5
54	85748.0
55	83746.0
56	78807.0
57	72449.5
58	66224.0
59	59738.5
60	51858.5
61	44028.0
62	36887.5
63	28803.5
64	21336.5
65	16212.5
66	12209.5
67	9235.5
68	7220.5
69	5544.5
70	4281.0
71	3379.0
72	2645.5
73	2176.0
74	1766.5
75	1411.5
76	1070.5
77	688.5
78	430.0
79	316.5
80	275.0
81	227.0
82	194.5
83	167.5
84	141.5
85	132.5
86	127.5
87	116.5
88	116.0
89	106.0
90	89.0
91	88.5
92	81.0
93	71.5
94	69.0
95	66.0
96	59.5
97	44.5
98	29.0
99	13.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35248812702214904
2	0.0
3	0.0
4	0.017355479590676837
5	0.17643813436911723
6	0.049404895576016176
7	0.023565747048043626
8	0.00754103905537396
9	0.005766676924697735
10-11	0.0019129841721353062
12-13	0.05816580859623003
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	2.495196746263443E-4
92-93	5.267637575445046E-4
94-95	1.1089763316726412E-4
96-97	5.544881658363206E-4
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0
106-107	1.9407085804271223E-4
108-109	5.544881658363206E-4
110-111	5.544881658363206E-4
112-113	8.594566570462969E-4
114-115	3.326928995017924E-4
116-117	7.208346155872169E-4
118-119	3.6041730779360845E-4
120-121	8.31732248754481E-5
122-123	0.0014416692311744338
124-125	8.31732248754481E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1803465.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.532670847055805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.23994765010578	52.24923524533271
2	9.619061959782673	12.222493946966162
3	3.0824992389446537	5.8751822860251215
4	1.49099370390196	3.7890724890014327
5	0.8648369239162058	2.7472699811774275
6	0.5661357879159792	2.15808712010427
7	0.3955552760126995	1.7591478206911804
8	0.285197697608397	1.4495497158793955
9	0.22561394420002845	1.290047080982978
>10	1.1691231374232285	12.976956264493758
>50	0.0493195918357811	2.0877821568734074
>100	0.011190722271822735	1.052981710526281
>500	2.6218304030722536E-4	0.10660600832972354
>1k	2.621830403072246E-4	0.2355881736161541
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.544881658363206E-5
2	0.0	0.0	0.0	0.0	1.1089763316726412E-4
3	0.0	0.0	0.0	0.0	1.1089763316726412E-4
4	0.0	0.0	0.0	0.0	1.1089763316726412E-4
5	0.0	0.0	0.0	0.0	1.1089763316726412E-4
6	0.0	0.0	0.0	0.0	1.1089763316726412E-4
7	0.0	0.0	0.0	0.0	1.1089763316726412E-4
8	0.0	0.0	0.0	0.0	2.2179526633452824E-4
9	0.0	0.0	0.0	0.0	2.772440829181603E-4
10-11	0.0	0.0	0.0	0.0	2.772440829181603E-4
12-13	0.0	0.0	0.0	0.0	3.049684912099763E-4
14-15	0.0	0.0	0.0	0.0	3.6041730779360845E-4
16-17	0.0	0.0	0.0	0.0	3.8814171608542446E-4
18-19	0.0	0.0	0.0	0.0	3.8814171608542446E-4
20-21	0.0	0.0	0.0	0.0	3.8814171608542446E-4
22-23	0.0	0.0	0.0	1.663464497508962E-4	3.8814171608542446E-4
24-25	0.0	0.0	0.0	3.326928995017924E-4	3.8814171608542446E-4
26-27	0.0	0.0	0.0	7.485590238790329E-4	4.435905326690565E-4
28-29	0.0	0.0	0.0	0.005045842309110518	4.435905326690565E-4
30-31	0.0	0.0	0.0	0.017300030774093204	4.713149409608725E-4
32-33	0.0	0.0	0.0	0.03834285666758157	5.544881658363206E-4
34-35	0.0	0.0	0.0	0.06481966658626588	5.544881658363206E-4
36-37	0.0	0.0	0.0	0.1049646097928155	6.099369824199527E-4
38-39	0.0	2.772440829181603E-5	0.0	0.1703110401366259	6.099369824199527E-4
40-41	0.0	5.544881658363206E-5	0.0	0.24461245435869286	6.099369824199527E-4
42-43	0.0	5.544881658363206E-5	0.0	0.3141729947628593	6.376613907117688E-4
44-45	0.0	5.544881658363206E-5	0.0	0.39426881031791583	6.931102072954007E-4
46-47	0.0	5.544881658363206E-5	0.0	0.48063034214692274	7.208346155872168E-4
48-49	0.0	5.544881658363206E-5	0.0	0.567851330632976	7.762834321708489E-4
50-51	0.0	5.544881658363206E-5	0.0	0.6628906022573213	7.762834321708489E-4
52-53	0.0	5.544881658363206E-5	0.0	0.7580684959231258	8.04007840462665E-4
54-55	0.0	8.31732248754481E-5	0.0	0.8667759008353364	8.594566570462969E-4
56-57	0.0	1.1089763316726412E-4	0.0	0.9827748251282947	8.87181065338113E-4
58-59	0.0	1.663464497508962E-4	0.0	1.1080614261990114	8.87181065338113E-4
60-61	0.0	1.663464497508962E-4	0.0	1.2372294444305822	8.87181065338113E-4
62-63	0.0	2.2179526633452824E-4	0.0	1.3720255175453917	9.426298819217451E-4
64-65	0.0	2.2179526633452824E-4	0.0	1.5102594172883865	9.426298819217451E-4
66-67	0.0	2.2179526633452824E-4	0.0	1.6682330957351543	9.703542902135612E-4
68-69	0.0	2.2179526633452824E-4	0.0	1.8437563246306414	9.980786985053771E-4
70-71	0.0	2.2179526633452824E-4	0.0	2.030424765659439	0.0010812519233808252
72-73	0.0	2.2179526633452824E-4	0.0	2.2089422306504423	0.0011089763316726412
74-75	0.0	2.2179526633452824E-4	0.0	2.394917561471944	0.0011367007399644574
76-77	0.0	2.772440829181603E-4	0.0	2.6062884502887496	0.0011644251482562733
78-79	0.0	2.772440829181603E-4	0.0	2.839811141330716	0.0011644251482562733
80-81	0.0	2.772440829181603E-4	0.0	3.080542178528555	0.0011644251482562733
82-83	0.0	2.772440829181603E-4	0.0	3.32748348318376	0.0013862204145908017
84-85	0.0	2.772440829181603E-4	0.0	3.5825480394684677	0.0013862204145908017
86-87	0.0	2.772440829181603E-4	0.0	3.8592099098125	0.0014139448228826176
88-89	0.0	2.772440829181603E-4	0.0	4.167255810342867	0.0014971180477580657
90-91	0.0	2.772440829181603E-4	0.0	4.500392300377329	0.0014971180477580657
92-93	0.0	2.772440829181603E-4	0.0	4.838546908312609	0.0014971180477580657
94-95	0.0	2.772440829181603E-4	0.0	5.181581012107249	0.0016080156809253298
96-97	0.0	2.772440829181603E-4	0.0	5.543994477297868	0.0016080156809253298
98-99	0.0	2.772440829181603E-4	0.0	5.948632216316923	0.0016080156809253298
100-101	0.0	2.772440829181603E-4	0.0	6.395771473247333	0.0016080156809253298
102-103	0.0	2.772440829181603E-4	0.0	6.854804501334931	0.0016080156809253298
104-105	0.0	2.772440829181603E-4	0.0	7.330555347622493	0.001635740089217146
106-107	0.0	2.772440829181603E-4	0.0	7.8282639252771755	0.001663464497508962
108-109	0.0	2.772440829181603E-4	0.0	8.34939962793844	0.00174663772238441
110-111	0.0	2.772440829181603E-4	0.0	8.90979309274092	0.0019407085804271221
112-113	0.0	2.772440829181603E-4	0.0	9.481359494084998	0.0019407085804271221
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2005	0.0	64.09954	1
GTATCAA	3140	0.0	46.640934	1
GTACATG	8350	0.0	38.443123	1
TACATGG	8560	0.0	37.45402	2
ACATGGG	8810	0.0	36.93233	3
TCAACGC	4165	0.0	33.76657	4
CATGGGG	7805	0.0	33.67097	4
CAACGCA	4340	0.0	32.679634	5
AACGCAG	4540	0.0	31.2374	6
ATCAACG	4545	0.0	30.943405	3
TATCAAC	4670	0.0	30.880793	2
ATGGGGG	4445	0.0	30.164818	5
GTGGTAT	1080	0.0	29.888275	1
ACGCAGA	5875	0.0	24.724598	1
GTAAGTA	720	0.0	24.002445	2
CGCAGAG	6480	0.0	23.08281	2
AGAGTAC	6420	0.0	22.927246	5
TGGTATC	1250	0.0	22.883432	2
ATGGGGA	3065	0.0	22.164825	5
CAGAGTA	6790	0.0	21.94119	4
>>END_MODULE