FastQCFastQC Report
Mon 15 Aug 2016
SRR2921291_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921291_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1606951
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16350.056.417041
GTATCAA27150.045.811321
GTACATG78350.037.5978131
TACATGG80300.036.4590532
TCAACGC34150.034.849384
ACATGGG84250.034.6084373
CAACGCA35150.034.1965145
CATGGGG78200.032.8725054
TATCAAC37800.032.4288252
ATCAACG37200.032.152073
AACGCAG38600.031.2932766
GTGGTAT10450.030.7520331
ATGGGGG47200.030.0048035
ACGCAGA47350.025.2536267
TGGTATC11450.024.9454612
TACCGTA1501.6888278E-423.7962047
AGTACAT55150.023.737392
GAGTACA57650.023.6391871
CGCAGAG51900.023.0396778
ATGGGGA30700.021.9026095
Produced by FastQC (version 0.11.5)