##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921291_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1606951
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.585057042809645	33.0	31.0	34.0	27.0	34.0
2	30.64075755888014	33.0	31.0	34.0	27.0	34.0
3	30.64203824509895	33.0	31.0	34.0	27.0	34.0
4	34.03246209747528	37.0	35.0	37.0	30.0	37.0
5	34.09287402042751	37.0	35.0	37.0	30.0	37.0
6	34.163520231793	37.0	35.0	37.0	30.0	37.0
7	34.08331056765265	37.0	35.0	37.0	30.0	37.0
8	34.10147851427953	37.0	35.0	37.0	30.0	37.0
9	35.7176734075899	39.0	37.0	39.0	30.0	39.0
10-11	35.7131838494142	39.0	37.0	39.0	30.0	39.0
12-13	35.670571473554574	39.0	37.0	39.0	30.0	39.0
14-15	37.02432432600621	41.0	38.0	41.0	31.0	41.0
16-17	37.03521638183118	41.0	38.0	41.0	31.0	41.0
18-19	37.03277044539628	41.0	38.0	41.0	30.0	41.0
20-21	37.03023396481909	41.0	38.0	41.0	30.0	41.0
22-23	37.006326888623235	41.0	38.0	41.0	30.0	41.0
24-25	37.00168268976465	41.0	38.0	41.0	30.0	41.0
26-27	36.9589863038761	41.0	38.0	41.0	30.0	41.0
28-29	36.87914877304908	41.0	38.0	41.0	30.0	41.0
30-31	36.805559099188464	40.0	38.0	41.0	30.0	41.0
32-33	36.714928457681665	40.0	38.0	41.0	30.0	41.0
34-35	36.60609657668466	40.0	38.0	41.0	30.0	41.0
36-37	36.50670462260517	40.0	37.5	41.0	29.0	41.0
38-39	36.405956062132574	40.0	37.0	41.0	29.0	41.0
40-41	36.28726358177692	40.0	37.0	41.0	28.5	41.0
42-43	36.11519953004168	40.0	37.0	41.0	27.5	41.0
44-45	35.911140103214095	40.0	36.0	41.0	27.0	41.0
46-47	35.71208300688696	40.0	35.5	41.0	27.0	41.0
48-49	35.50330159413697	40.0	35.0	41.0	26.0	41.0
50-51	35.322851474624926	40.0	35.0	41.0	26.0	41.0
52-53	35.11955498331935	39.0	35.0	41.0	26.0	41.0
54-55	34.902050218083815	39.0	35.0	41.0	25.0	41.0
56-57	34.64733896677621	38.5	35.0	41.0	24.0	41.0
58-59	34.37216847308972	38.0	34.5	40.0	23.0	41.0
60-61	34.0737555158807	37.5	34.0	40.0	23.0	41.0
62-63	33.746839511596804	37.0	34.0	40.0	22.0	41.0
64-65	33.38807219386278	36.5	34.0	39.5	21.5	41.0
66-67	33.03834031031438	36.0	33.5	39.0	20.5	41.0
68-69	32.67398196958091	35.0	33.0	39.0	20.0	40.0
70-71	32.59101522075035	35.0	34.0	38.5	20.0	40.0
72-73	32.29556128344922	35.0	33.0	37.0	19.5	39.5
74-75	31.975277093078756	35.0	33.0	37.0	18.5	39.0
76-77	31.68208707048317	35.0	33.0	36.0	18.0	39.0
78-79	31.408746439686087	35.0	33.0	36.0	17.0	37.5
80-81	31.14703590837555	35.0	33.0	35.5	13.5	37.0
82-83	30.94034821223547	35.0	33.0	35.0	11.5	37.0
84-85	30.736314921861336	35.0	33.0	35.0	7.5	36.0
86-87	30.575525327156832	35.0	32.5	35.0	7.0	36.0
88-89	30.436315419698545	35.0	32.0	35.0	6.0	36.0
90-91	30.314477541630083	35.0	32.0	35.0	2.0	35.0
92-93	30.209020996906567	35.0	32.0	35.0	2.0	35.0
94-95	30.085511008114125	35.0	32.0	35.0	2.0	35.0
96-97	29.99087713315465	35.0	32.0	35.0	2.0	35.0
98-99	29.896023898675192	35.0	32.0	35.0	2.0	35.0
100-101	29.790374753181645	35.0	31.5	35.0	2.0	35.0
102-103	29.009520203167362	34.0	29.5	35.0	2.0	35.0
104-105	29.561954596001993	34.5	31.0	35.0	2.0	35.0
106-107	29.620336276588397	35.0	31.0	35.0	2.0	35.0
108-109	29.53175765782529	35.0	31.0	35.0	2.0	35.0
110-111	29.432065445679427	35.0	31.0	35.0	2.0	35.0
112-113	29.3342544358851	35.0	31.0	35.0	2.0	35.0
114-115	29.200603192007723	35.0	31.0	35.0	2.0	35.0
116-117	29.057662305820152	34.5	31.0	35.0	2.0	35.0
118-119	29.028008632497198	34.5	31.0	35.0	2.0	35.0
120-121	29.165333603824884	35.0	31.0	35.0	2.0	35.0
122-123	29.00923985858934	35.0	31.0	35.0	2.0	35.0
124-125	28.464980575014422	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	59203.0
3	7591.0
4	4956.0
5	4385.0
6	4643.0
7	5192.0
8	5320.0
9	5538.0
10	6078.0
11	6644.0
12	6857.0
13	6989.0
14	7115.0
15	6197.0
16	4673.0
17	4188.0
18	4162.0
19	4036.0
20	4017.0
21	4364.0
22	5083.0
23	6770.0
24	9457.0
25	11187.0
26	12324.0
27	13770.0
28	16400.0
29	20090.0
30	24276.0
31	30437.0
32	39100.0
33	53307.0
34	81805.0
35	139034.0
36	303130.0
37	531017.0
38	147545.0
39	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.59936766174882	17.211052227031836	11.546272409575941	25.643307701643398
2	17.573773552613154	19.19708733332296	37.04793763905961	26.18120147500428
3	20.791520930058876	22.70366820597717	27.905375969329967	28.59943489463399
4	14.740143954741077	14.438168855861658	33.151725060759105	37.669962128638154
5	15.22864090026207	37.037168421373636	32.08621246548309	15.647978212881211
6	35.48443320120312	32.606928370814494	16.22462801027827	15.684010417704123
7	30.810211650452807	31.61792758438914	18.527987811443328	19.04387295371473
8	28.59777254296677	33.735051485438134	18.524488646441764	19.142687325153336
9	27.977896742763043	15.862366833414033	17.859538891053273	38.30019753276965
10-11	27.167472494098654	24.99727725961003	25.615012599286676	22.22023764700464
12-13	26.800352490459606	22.600587981967227	25.892490409819946	24.706569117753222
14-15	24.488840194901798	24.13818747800447	22.949036335139237	28.423935991954497
16-17	24.896025540097767	27.030465776953548	23.863468760987875	24.210039921960814
18-19	24.752087370045984	26.12500789580217	25.471842247006833	23.651062487145012
20-21	25.54407674281765	24.818817411575957	25.387406040363476	24.249699805242912
22-23	25.54662169310523	24.47556429580489	25.276873673663708	24.70094033742618
24-25	25.02286282027785	24.989257041577506	25.1701681502477	24.81771198789694
26-27	24.667314503740307	25.315867247541906	25.374770013171442	24.64204823554634
28-29	24.627659839289446	25.516502952334484	25.397700116500214	24.458137091875855
30-31	25.163353035166136	24.9847140952378	25.350829017236897	24.50110385235916
32-33	24.829672769456202	24.97094440712121	25.25168289060441	24.94769993281818
34-35	24.941437780286503	25.26819754076018	25.319851783440523	24.4705128955128
36-37	24.714207388755742	25.5229651609643	25.324937595178632	24.437889855101325
38-39	24.72045383330258	25.627417197235204	25.434648197353138	24.217480772109077
40-41	24.790988059838963	25.173165318891794	25.489530421708118	24.546316199561126
42-43	24.633614774773203	25.012368943651758	25.347621771083368	25.006394510491663
44-45	24.539353659079232	25.29406218581687	25.34453374639939	24.822050408704506
46-47	24.567194637087628	25.38297070568551	25.162472184855307	24.88736247237156
48-49	24.348275255821626	25.620280894243802	25.40971134203472	24.62173250789986
50-51	24.36931540608576	25.441465213319102	25.601341743083584	24.58787763751155
52-53	24.649490034371105	25.250968807117925	25.370860049300724	24.72868110921025
54-55	24.410340664401378	25.127266843408886	25.377226391844964	25.085166100344775
56-57	24.46248537502178	25.50132558313211	25.101876975927905	24.9343120659182
58-59	24.340624547832164	25.638107032892147	25.36773205836913	24.65353636090656
60-61	24.682551733923464	25.290065941615907	25.59547220818532	24.431910116275308
62-63	24.540939839563872	25.32270812200116	25.507570618656146	24.628781419778825
64-65	24.542659779700042	25.13541601842056	25.28346505694194	25.03845914493746
66-67	24.498295004248877	25.639066137157023	25.08031791737546	24.782320941218643
68-69	24.296686890386773	25.608656391133263	25.32854469496079	24.766112023519177
70-71	24.204390863797094	25.361071939320993	25.61995840391516	24.814578792966753
72-73	24.33545544499001	25.560897136705062	25.332101139468165	24.77154627883676
74-75	24.291070667342918	25.61312344614925	25.24424433457071	24.85156155193712
76-77	24.363855194501912	25.42755820028359	25.18180344411382	25.026783161100674
78-79	24.27435083321613	25.475945699375075	25.31694448835215	24.932758979056647
80-81	24.359459843780485	25.731569155746868	25.177958504716052	24.731012495756595
82-83	24.462909540907184	25.548712392466	25.396398165259228	24.59197990136759
84-85	24.550740566252035	25.568221775927597	25.11533714917311	24.76570050864725
86-87	24.27926465998506	25.755477258745923	25.160088209933917	24.8051698713351
88-89	24.247984606212526	25.587161969578354	25.17484171208589	24.99001171212323
90-91	24.40295604823026	25.440466744457407	25.326052119797744	24.830525087514584
92-93	24.379058446609918	25.476371548025494	25.3978631271183	24.74670687824629
94-95	24.436326409320113	25.29543096031204	25.38168681222082	24.886555818147023
96-97	24.38153406872027	25.291842233581452	25.324422142264357	25.002201555433924
98-99	24.26059457378838	25.476639521398564	25.204274080599387	25.058491824213668
100-101	24.15496713717871	25.624007751904216	25.3108136449944	24.91021146592268
102-103	23.90001453132434	25.559417091637894	25.575939860854014	24.964628516183758
104-105	24.066817663167857	25.56051927943615	25.46508592269307	24.907577134702922
106-107	24.24389899340042	25.478740799908888	25.347707666978565	24.929652539712123
108-109	24.15472225688278	25.626760622545863	25.333361130930733	24.885155989640623
110-111	23.951418873589	26.003679275695497	25.297478650616927	24.747423200098577
112-113	23.87413457798522	26.040606767794632	25.38893815635939	24.696320497860754
114-115	23.88949512279893	26.036278821132786	25.353417774612293	24.720808281455994
116-117	23.902441149363344	25.962392347534735	25.280430043555725	24.8547364595462
118-119	24.01408683327963	25.92505221327573	25.25449102729425	24.80636992615039
120-121	23.866398430413387	26.11062100426782	25.321377838146493	24.7016027271723
122-123	23.709233021505394	26.255902198145836	25.31659118594705	24.718273594401722
124-125	23.665301431023757	26.107510206596256	25.33249031614078	24.894698046239203
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	344.0
1	311.0
2	265.0
3	223.0
4	183.5
5	168.5
6	170.5
7	232.0
8	324.0
9	360.5
10	356.0
11	374.5
12	418.0
13	456.5
14	485.5
15	518.0
16	592.5
17	635.5
18	697.5
19	770.5
20	817.5
21	901.5
22	994.5
23	1159.5
24	1327.5
25	1526.5
26	1883.5
27	2364.0
28	3014.0
29	4000.5
30	5408.5
31	7078.0
32	9474.5
33	11937.0
34	14156.5
35	16803.0
36	20044.5
37	23283.5
38	26095.0
39	29459.0
40	33134.5
41	36370.0
42	39728.0
43	44612.5
44	50166.0
45	55346.5
46	59380.5
47	63665.5
48	68993.5
49	72580.0
50	74028.5
51	74827.5
52	74986.0
53	74164.0
54	73903.5
55	72273.0
56	67533.0
57	61539.5
58	56416.0
59	51461.5
60	44427.0
61	37217.5
62	30850.5
63	23976.0
64	17790.0
65	13529.0
66	10421.0
67	8047.0
68	6260.5
69	4873.0
70	3841.0
71	2942.0
72	2185.0
73	1692.0
74	1292.0
75	958.5
76	693.0
77	441.5
78	279.5
79	176.0
80	116.0
81	91.0
82	63.0
83	40.5
84	35.0
85	33.5
86	32.0
87	21.5
88	11.0
89	8.0
90	7.0
91	5.5
92	3.5
93	7.0
94	9.0
95	5.5
96	3.5
97	3.5
98	2.0
99	1.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013441604628890364
2	0.01095241858650326
3	0.010641270331204872
4	0.012197011607696812
5	0.00808985463775809
6	0.028314491232153315
7	0.012321470909816166
8	0.011574715097100036
9	0.008027624986698413
10-11	0.0070941802208032475
12-13	0.006160735454908083
14-15	0.005631783420900823
16-17	0.004107156969938723
18-19	0.005258405514542758
20-21	0.004822797957125015
22-23	0.0050406017358338865
24-25	0.005258405514542758
26-27	0.0040760421444088835
28-29	0.005413979642191952
30-31	0.004885027608184693
32-33	0.007311983999512119
34-35	0.0070319505697435695
36-37	0.006471883710206472
38-39	0.006565228186795988
40-41	0.00653411336126615
42-43	0.006471883710206472
44-45	0.006565228186795988
46-47	0.00855657702070567
48-49	0.007374213650571797
50-51	0.005351749991132275
52-53	0.004853912782654853
54-55	0.005662898246430663
56-57	0.006907491267624215
58-59	0.006751917139975022
60-61	0.004667223829475821
62-63	0.0050406017358338865
64-65	0.003173712204043558
66-67	0.0034537456338121077
68-69	0.005725127897490341
70-71	0.004293845923117755
72-73	0.0037960087146403343
74-75	0.0034848604593419466
76-77	0.004013812493349206
78-79	0.002800334297685493
80-81	0.0028314491232153314
82-83	0.00469833865500566
84-85	0.008712151148354864
86-87	0.006378539233616955
88-89	0.004293845923117755
90-91	0.004729453480535499
92-93	0.0072808691739822805
94-95	0.006440768884676633
96-97	0.008245428765407284
98-99	0.006378539233616955
100-101	0.007467558127161313
102-103	0.004822797957125015
104-105	0.004387190399707272
106-107	0.006565228186795988
108-109	0.006067390978318567
110-111	0.0070319505697435695
112-113	0.004729453480535499
114-115	0.002209152612618555
116-117	0.002738104646625815
118-119	0.1539561567216424
120-121	0.009863399692958902
122-123	0.0017735450552008119
124-125	0.0015246264509621015
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1606951.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.23484023796318
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.58319858234063	49.344992910536895
2	10.3046030988414	12.620014489463468
3	3.4158661366155467	6.275100514497646
4	1.666558711091429	4.082058256834779
5	0.9790083273636251	2.997470925887357
6	0.6476042541033236	2.37935658224654
7	0.45607909246952694	1.9549551254272701
8	0.3196873811100503	1.5660804566693425
9	0.2458598433420086	1.3549669405180698
>10	1.3181704315447398	13.864539341265711
>50	0.0498561944104347	2.0575420620161995
>100	0.01310174629919263	1.3349010863465371
>500	4.06200468094327E-4	0.16802130829015002
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.222965105967761E-5
7	0.0	0.0	0.0	0.0	6.222965105967761E-5
8	0.0	0.0	0.0	0.0	6.222965105967761E-5
9	0.0	0.0	0.0	0.0	1.2445930211935522E-4
10-11	0.0	0.0	0.0	0.0	1.2445930211935522E-4
12-13	0.0	0.0	0.0	0.0	2.1780377870887164E-4
14-15	0.0	0.0	0.0	0.0	4.0449273188790445E-4
16-17	0.0	0.0	0.0	6.222965105967761E-5	4.978372084774209E-4
18-19	0.0	0.0	0.0	6.222965105967761E-5	4.978372084774209E-4
20-21	0.0	0.0	0.0	1.2445930211935522E-4	4.978372084774209E-4
22-23	0.0	0.0	0.0	2.1780377870887164E-4	5.600668595370985E-4
24-25	0.0	0.0	0.0	5.289520340072597E-4	6.222965105967762E-4
26-27	0.0	0.0	0.0	0.0013690523233129076	6.222965105967762E-4
28-29	0.0	0.0	0.0	0.005756242723020179	6.222965105967762E-4
30-31	0.0	0.0	0.0	0.02448736769198314	6.222965105967762E-4
32-33	0.0	0.0	0.0	0.054824322583575974	6.222965105967762E-4
34-35	0.0	0.0	0.0	0.09707825565309708	6.222965105967762E-4
36-37	6.222965105967761E-5	0.0	0.0	0.14574184278176497	6.222965105967762E-4
38-39	6.222965105967761E-5	0.0	0.0	0.21354104761128373	6.534113361266149E-4
40-41	6.222965105967761E-5	0.0	0.0	0.291857063469888	6.845261616564537E-4
42-43	6.222965105967761E-5	0.0	0.0	0.381810024076652	6.845261616564537E-4
44-45	6.222965105967761E-5	0.0	0.0	0.4907741430821475	6.845261616564537E-4
46-47	6.222965105967761E-5	0.0	0.0	0.6108462548017954	7.156409871862926E-4
48-49	6.222965105967761E-5	0.0	0.0	0.718254632530799	7.467558127161314E-4
50-51	6.222965105967761E-5	0.0	0.0	0.8158307253923736	8.08985463775809E-4
52-53	6.222965105967761E-5	0.0	0.0	0.9275018342189649	8.08985463775809E-4
54-55	6.222965105967761E-5	0.0	0.0	1.0776308674004373	8.08985463775809E-4
56-57	9.334447658951642E-5	0.0	0.0	1.2478289630486556	8.08985463775809E-4
58-59	1.2445930211935522E-4	0.0	0.0	1.4015984308171188	8.08985463775809E-4
60-61	1.2445930211935522E-4	0.0	0.0	1.539219304135596	8.08985463775809E-4
62-63	1.2445930211935522E-4	0.0	0.0	1.6908107341169707	9.334447658951642E-4
64-65	1.2445930211935522E-4	0.0	0.0	1.87429486026643	9.334447658951642E-4
66-67	1.2445930211935522E-4	0.0	0.0	2.1060691956381996	9.334447658951642E-4
68-69	1.2445930211935522E-4	0.0	0.0	2.3305626618359865	9.334447658951642E-4
70-71	1.2445930211935522E-4	0.0	0.0	2.5423923940431292	9.334447658951642E-4
72-73	1.2445930211935522E-4	0.0	0.0	2.743238592838239	0.0010579040680145195
74-75	1.2445930211935522E-4	0.0	0.0	2.9788711665757077	0.001151248544604036
76-77	1.2445930211935522E-4	0.0	0.0	3.2823962896192853	0.0012445930211935523
78-79	1.2445930211935522E-4	0.0	0.0	3.596904946074896	0.0012445930211935523
80-81	1.2445930211935522E-4	0.0	0.0	3.8846548525748448	0.0012445930211935523
82-83	1.2445930211935522E-4	0.0	0.0	4.150873299808146	0.0012445930211935523
84-85	1.2445930211935522E-4	0.0	0.0	4.4481443429202265	0.0012445930211935523
86-87	1.2445930211935522E-4	0.0	0.0	4.824198124273858	0.0012757078467233911
88-89	1.2445930211935522E-4	0.0	0.0	5.248666574151919	0.0013690523233129074
90-91	1.2445930211935522E-4	0.0	0.0	5.657079774056583	0.0013690523233129074
92-93	1.2445930211935522E-4	0.0	0.0	6.019536376653676	0.0013690523233129074
94-95	1.2445930211935522E-4	0.0	0.0	6.421477692848133	0.0014312819743725852
96-97	1.2445930211935522E-4	0.0	0.0	6.9059666411732525	0.001462396799902424
98-99	1.2445930211935522E-4	0.0	0.0	7.476674771041556	0.0015246264509621015
100-101	1.2445930211935522E-4	0.0	0.0	8.030798698902457	0.0015557412764919403
102-103	1.2445930211935522E-4	0.0	0.0	8.534298805626307	0.0015557412764919403
104-105	1.2445930211935522E-4	0.0	0.0	9.04361738472424	0.0015557412764919403
106-107	1.2445930211935522E-4	0.0	0.0	9.643262302335293	0.0015557412764919403
108-109	1.2445930211935522E-4	0.0	0.0	10.358156533708868	0.0015868561020217792
110-111	1.2445930211935522E-4	0.0	0.0	11.081887375532919	0.001617970927551618
112-113	1.2445930211935522E-4	0.0	0.0	11.73231168840867	0.001617970927551618
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1635	0.0	56.41704	1
GTATCAA	2715	0.0	45.81132	1
GTACATG	7835	0.0	37.597813	1
TACATGG	8030	0.0	36.459053	2
TCAACGC	3415	0.0	34.84938	4
ACATGGG	8425	0.0	34.608437	3
CAACGCA	3515	0.0	34.196514	5
CATGGGG	7820	0.0	32.872505	4
TATCAAC	3780	0.0	32.428825	2
ATCAACG	3720	0.0	32.15207	3
AACGCAG	3860	0.0	31.293276	6
GTGGTAT	1045	0.0	30.752033	1
ATGGGGG	4720	0.0	30.004803	5
ACGCAGA	4735	0.0	25.253626	7
TGGTATC	1145	0.0	24.945461	2
TACCGTA	150	1.6888278E-4	23.796204	7
AGTACAT	5515	0.0	23.73739	2
GAGTACA	5765	0.0	23.639187	1
CGCAGAG	5190	0.0	23.039677	8
ATGGGGA	3070	0.0	21.902609	5
>>END_MODULE