FastQCFastQC Report
Mon 15 Aug 2016
SRR2921291_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921291_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1606951
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22940.14275481953090044No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23300.068.996821
GTATCAA36950.043.7073022
GTACATG77650.038.7132151
GTGGTAT10950.037.6589051
TACATGG80200.036.8558542
ACATGGG83250.035.4339943
CATGGGG77000.033.4342964
TCAACGC48900.033.2700085
TGGTATC12500.032.895632
CAACGCA50250.032.5961076
ATCAACG51200.031.7754614
TATCAAC52100.031.4553223
AACGCAG52450.031.2220657
ATGGGGG43250.030.4512635
ATGGGGA29150.025.3502525
ACGCAGA66000.024.80598
CGCAGAG70800.023.1234259
TGGGGGG32600.022.1073596
TACCGTA1653.1982054E-421.6541797
CAGAGTA73250.020.80518210-11
Produced by FastQC (version 0.11.5)