FastQCFastQC Report
Mon 15 Aug 2016
SRR2921290_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921290_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378723
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11650.055.1619071
GTATCAA20050.049.5613941
GTACATG69250.041.8456841
TCAACGC22950.040.4452554
TACATGG71050.040.114112
CAACGCA23650.039.2467235
ACATGGG72050.039.227023
AACGCAG24650.038.1373146
ATCAACG25500.036.867413
CATGGGG64450.036.0053754
TATCAAC26250.035.5873832
ATGGGGG37950.034.0218475
GAGTACA47000.028.6122531
ACGCAGA33350.028.1853837
AGTACAT46350.027.9854582
CGCAGAG35450.026.5157268
TGGGGGG28250.025.695166
TACCGAC1451.3414027E-424.617617
AGAGTAC35700.023.9996135
ATGGGGA26250.022.6663025
Produced by FastQC (version 0.11.5)