##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921290_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1378723
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.621368469228408	31.0	31.0	34.0	27.0	34.0
2	30.58942441665222	31.0	31.0	34.0	27.0	34.0
3	30.77733525878657	33.0	31.0	34.0	27.0	34.0
4	34.21576052622608	37.0	35.0	37.0	31.0	37.0
5	34.26269671282774	37.0	35.0	37.0	31.0	37.0
6	34.28857065559942	37.0	35.0	37.0	31.0	37.0
7	34.28294153357854	37.0	35.0	37.0	31.0	37.0
8	34.261489798893614	37.0	35.0	37.0	31.0	37.0
9	35.872312277375514	39.0	37.0	39.0	31.0	39.0
10-11	35.88320025124699	39.0	37.0	39.0	31.0	39.0
12-13	35.80082075949991	39.0	37.0	39.0	30.0	39.0
14-15	37.14731494288556	41.0	38.0	41.0	31.0	41.0
16-17	37.13505976182308	41.0	38.0	41.0	31.0	41.0
18-19	37.12669042294935	41.0	38.0	41.0	31.0	41.0
20-21	37.170473691959884	41.0	38.0	41.0	31.0	41.0
22-23	37.1273069354758	41.0	38.0	41.0	30.5	41.0
24-25	37.12097934102789	41.0	38.0	41.0	30.0	41.0
26-27	37.098938655553	41.0	38.0	41.0	30.0	41.0
28-29	37.04381554525456	41.0	38.0	41.0	30.0	41.0
30-31	36.97136698234526	40.0	38.0	41.0	30.0	41.0
32-33	36.90111356668453	40.0	38.0	41.0	30.0	41.0
34-35	36.79543461594534	40.0	38.0	41.0	30.0	41.0
36-37	36.691179446487794	40.0	38.0	41.0	30.0	41.0
38-39	36.593967026008855	40.0	37.5	41.0	30.0	41.0
40-41	36.492024503834344	40.0	37.0	41.0	29.0	41.0
42-43	36.29789413827143	40.0	37.0	41.0	28.5	41.0
44-45	36.04117360775152	40.0	36.0	41.0	28.0	41.0
46-47	35.831992720800336	40.0	36.0	41.0	27.0	41.0
48-49	35.63709207723379	40.0	35.0	41.0	27.0	41.0
50-51	35.4659373202594	39.5	35.0	41.0	27.0	41.0
52-53	35.27301423128503	39.0	35.0	41.0	26.0	41.0
54-55	35.070828585582454	39.0	35.0	41.0	26.0	41.0
56-57	34.83119778229565	39.0	35.0	40.5	26.0	41.0
58-59	34.566327318830545	38.0	34.5	40.0	25.0	41.0
60-61	34.280057705572474	38.0	34.0	40.0	24.5	41.0
62-63	33.95240341968619	37.0	34.0	40.0	23.5	41.0
64-65	33.60047957421469	37.0	34.0	39.5	22.5	41.0
66-67	33.25505232015423	36.0	33.5	39.0	22.0	41.0
68-69	32.89527265447809	36.0	33.0	39.0	22.0	40.0
70-71	32.84338841087006	35.0	33.0	39.0	22.0	40.0
72-73	32.552607376536116	35.0	33.0	37.5	21.5	40.0
74-75	32.22105709413711	35.0	33.0	37.0	20.5	39.0
76-77	31.91544168045358	35.0	33.0	36.5	20.0	39.0
78-79	31.62264537546701	35.0	33.0	36.0	20.0	38.5
80-81	31.34257062513645	35.0	33.0	36.0	18.5	37.0
82-83	31.117221878506413	35.0	33.0	35.0	18.0	37.0
84-85	30.893924305317313	35.0	32.0	35.0	17.0	36.5
86-87	30.717311961866162	35.0	32.0	35.0	15.0	36.0
88-89	30.57943908965035	35.0	32.0	35.0	12.5	36.0
90-91	30.446536396360983	35.0	32.0	35.0	10.0	35.5
92-93	30.329274625867562	35.0	32.0	35.0	7.0	35.0
94-95	30.20290986659394	35.0	32.0	35.0	7.0	35.0
96-97	30.108344098125585	35.0	32.0	35.0	6.0	35.0
98-99	30.02235655748109	35.0	31.5	35.0	2.0	35.0
100-101	29.92541757844034	35.0	31.0	35.0	2.0	35.0
102-103	29.06762272044493	33.5	29.5	34.5	2.0	35.0
104-105	29.67004866097106	34.0	31.0	35.0	2.0	35.0
106-107	29.749550489837333	35.0	31.0	35.0	2.0	35.0
108-109	29.682925069067537	35.0	31.0	35.0	2.0	35.0
110-111	29.598166564277236	34.5	31.0	35.0	2.0	35.0
112-113	29.503701613739672	34.0	31.0	35.0	2.0	35.0
114-115	29.369438966347843	34.0	31.0	35.0	2.0	35.0
116-117	29.2371419059521	34.0	31.0	35.0	2.0	35.0
118-119	29.229919280377565	34.5	31.0	35.0	2.0	35.0
120-121	29.384378878135784	35.0	31.5	35.0	2.0	35.0
122-123	29.24699994124998	35.0	31.0	35.0	2.0	35.0
124-125	28.66406921477338	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	44216.0
3	6752.0
4	4118.0
5	3414.0
6	3671.0
7	4290.0
8	4222.0
9	4617.0
10	5178.0
11	5721.0
12	6155.0
13	6042.0
14	5776.0
15	4922.0
16	4144.0
17	3801.0
18	3548.0
19	3637.0
20	3535.0
21	3732.0
22	4270.0
23	5671.0
24	8206.0
25	10381.0
26	10613.0
27	12127.0
28	14400.0
29	17497.0
30	21236.0
31	26679.0
32	34435.0
33	47229.0
34	74301.0
35	121351.0
36	257599.0
37	449355.0
38	131787.0
39	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.969840453430585	17.267998047108783	11.41788462417363	25.344276875287004
2	16.691197219113086	19.421668952880303	38.344626575837125	25.542507252169482
3	20.7123525362353	22.9887875673076	28.226430642431698	28.0724292540254
4	13.565510207338605	14.290999464642464	34.77778309750036	37.36570723051857
5	14.018402794149114	38.319956187277626	32.96747775815232	14.694163260420936
6	34.65176430470759	33.74478072705243	16.576156946394303	15.027298021845672
7	30.496259429407928	32.05849986906371	18.891169090620245	18.554071610908117
8	27.94547874752003	35.64417556191492	18.30292083319732	18.107424857367732
9	27.795343843958204	16.074945325165658	17.764261699326493	38.365449131549646
10-11	26.973626467684642	25.708010274064513	26.096660638886654	21.221702619364187
12-13	26.104916972102078	22.89735556888599	26.674067717804522	24.32365974120741
14-15	23.599192684107738	24.356858286321508	23.685509477620194	28.35843955195056
16-17	24.286367607586595	27.572400471904597	24.358611225251032	23.78262069525778
18-19	24.116957121573257	27.01633201367723	25.74801870246228	23.118692162287235
20-21	24.882775198572233	25.931188706089888	25.740132861423948	23.44590323391394
22-23	24.773048485351833	25.408850011986335	25.65593892881714	24.162162573844697
24-25	24.257796137156163	25.548698996661585	25.82643496615342	24.36706990002883
26-27	24.040057635528903	25.861169168546205	25.793241062811916	24.305532133112976
28-29	24.140260908355668	26.46526118036188	25.519658795411438	23.87481911587102
30-31	24.78338465756624	25.92054493750605	25.60683304398096	23.68923736094675
32-33	24.220177496980668	25.884275160215143	25.71910939602427	24.17643794677992
34-35	24.247573211665994	26.15121253279995	25.802168468888333	23.799045786645728
36-37	24.239805255518714	26.101331252035543	25.817897081661684	23.840966410784063
38-39	24.323496045355107	26.21544400536184	25.689052626081505	23.77200732320155
40-41	24.388830336269347	25.98784877998182	25.71489605237675	23.908424831372084
42-43	24.025850385039302	26.019430222348273	25.606337616705915	24.34838177590651
44-45	23.84159161310188	26.076859831143533	26.01241162853028	24.069136927224307
46-47	23.863464087039215	25.89545748322389	25.832785070618897	24.408293359118
48-49	23.822385252423995	26.1931013272035	25.84311188128938	24.14140153908312
50-51	23.90065342979012	26.208529585314892	25.843316842545434	24.047500142349556
52-53	24.12331412141372	26.13704776674128	25.777495216362578	23.96214289548242
54-55	23.760619595000154	25.867547243030735	26.09788251849192	24.27395064347719
56-57	24.00846203310029	26.058651768768744	25.676714212712128	24.256171985418842
58-59	23.883738246034742	26.24292731083492	25.94582087078445	23.92751357234589
60-61	24.305364396953696	26.067118338931184	25.95414587193528	23.67337139217984
62-63	24.151434989710957	26.17232352495302	25.89248475502502	23.783756730311005
64-65	23.941214742518017	25.92438123740189	25.883472512455764	24.250931507624326
66-67	23.870211757435484	26.16302664185543	25.803696516187806	24.16306508452128
68-69	23.794130820716756	26.05381743272821	25.97917879363214	24.172872952922894
70-71	23.769213835303297	25.967505439152394	26.131360018394652	24.13192070714965
72-73	23.80103737584964	26.478543466860476	25.670881595391503	24.04953756189838
74-75	23.760884994329704	26.33745214156631	25.84843206396284	24.05323080014115
76-77	23.727356734698617	25.935288169868553	25.966804142555194	24.370550952877636
78-79	23.629509278769262	25.96020845951033	26.13639083619536	24.273891425525047
80-81	23.875986310158257	26.2893703535938	25.651734458849056	24.182908877398887
82-83	24.025880296375814	26.269878976248588	25.784804755359392	23.919435972016203
84-85	24.03041789239999	26.394742769185907	25.568757790985252	24.006081547428852
86-87	23.6402845135112	26.512221517821267	25.77449852535191	24.07299544331563
88-89	23.61195129770749	26.13660926318416	25.90689378542384	24.344545653684516
90-91	23.98665079133085	25.922082952377256	25.933543368426626	24.15772288786527
92-93	23.980136600764826	26.166133281493902	25.85277553720543	24.00095458053584
94-95	23.95574018302201	26.128582536891642	25.788100087623096	24.127577192463253
96-97	23.653107248735854	26.03139128927266	25.983697942621458	24.331803519370027
98-99	23.561404170498957	26.0879594033523	25.7864294853754	24.564206940773346
100-101	23.59721430364119	26.257313504722855	25.93568613114331	24.20978606049264
102-103	23.472233253509128	26.19695507467607	25.998174306905753	24.332637364909047
104-105	23.609301785792	26.266338328521897	25.84973815153845	24.27462173414765
106-107	23.591351098847692	26.15042078127063	25.948371784660964	24.30985633522072
108-109	23.5771122265421	26.10946294899321	25.98876428944467	24.32466053502002
110-111	23.441596028498022	26.532508548391075	25.879864096177197	24.146031326933706
112-113	23.419837871086045	26.605331569296474	25.888546526472155	24.086284033145326
114-115	23.53733912770378	26.708056063181512	25.723755335210228	24.030849473904482
116-117	23.441879728452076	26.54127455186578	25.79897489534438	24.217870824337766
118-119	23.45269229651585	26.446001511056867	25.882733269404007	24.218572923023277
120-121	23.338067687236684	26.55435753995427	26.02090853170228	24.086666241106766
122-123	23.335795798866975	26.649570845568594	25.848526092886807	24.166107262677617
124-125	23.27723610700602	26.644484054812207	25.815990701435947	24.262289136745828
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	381.0
1	342.5
2	261.0
3	205.5
4	196.0
5	168.0
6	153.5
7	174.0
8	201.0
9	229.5
10	241.5
11	256.0
12	281.0
13	313.5
14	339.0
15	381.0
16	411.0
17	442.0
18	494.5
19	560.5
20	639.0
21	746.0
22	893.0
23	1132.0
24	1434.0
25	1871.5
26	2609.0
27	3453.5
28	4437.5
29	5725.0
30	7390.0
31	9279.0
32	11517.0
33	13862.0
34	16406.5
35	19079.0
36	21922.5
37	24970.5
38	27427.5
39	30013.5
40	33019.0
41	35710.0
42	38124.0
43	41637.0
44	45449.5
45	48919.5
46	52077.0
47	54990.5
48	58778.5
49	61548.0
50	62920.5
51	63373.0
52	62429.0
53	60948.0
54	59528.5
55	57162.5
56	52864.0
57	47820.5
58	43488.0
59	39033.0
60	33279.5
61	27498.5
62	22435.0
63	17320.5
64	12645.0
65	9140.0
66	6682.5
67	4977.5
68	3694.0
69	2802.0
70	2151.5
71	1615.0
72	1175.0
73	880.5
74	629.5
75	429.0
76	298.5
77	181.0
78	124.0
79	81.5
80	51.5
81	35.0
82	26.5
83	17.5
84	14.5
85	11.5
86	9.0
87	7.5
88	7.0
89	4.5
90	3.5
91	3.5
92	3.0
93	2.5
94	3.5
95	2.5
96	2.0
97	1.5
98	0.5
99	1.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01842284490793292
2	0.012330250528931483
3	0.011895065216145665
4	0.014868831520182082
5	0.009283953339430762
6	0.030317910124078588
7	0.013780868238217541
8	0.012910497612645904
9	0.008558644484787735
10-11	0.007688273859216101
12-13	0.006527779691787256
14-15	0.005838736279876379
16-17	0.003952933257804505
18-19	0.00587500172260853
20-21	0.004968365654304745
22-23	0.005040896539769047
24-25	0.005476081852554864
26-27	0.004315587685126019
28-29	0.005657409066215621
30-31	0.005040896539769047
32-33	0.007833335630144708
34-35	0.007289353989162435
36-37	0.006962965004573073
38-39	0.006600310577251558
40-41	0.006962965004573073
42-43	0.0070717613327695265
44-45	0.0069992304473052245
46-47	0.009138891568502156
48-49	0.007978397401073312
50-51	0.005403550967090561
52-53	0.004714507555179684
54-55	0.005838736279876379
56-57	0.006854168676376619
58-59	0.006709106905448012
60-61	0.004968365654304745
62-63	0.005222223753429804
64-65	0.003191358960429325
66-67	0.003372686174090082
68-69	0.005838736279876379
70-71	0.004460649456054624
72-73	0.0037716060441437475
74-75	0.0034452170595543853
76-77	0.004134260471465262
78-79	0.0027561736476435076
80-81	0.0026473773194470537
82-83	0.004932100211572593
84-85	0.009175157011234308
86-87	0.006455248806322952
88-89	0.004424384013322473
90-91	0.004678242112447533
92-93	0.007760804744680404
94-95	0.006600310577251558
96-97	0.008993829797573552
98-99	0.006310187035394346
100-101	0.00790586651560901
102-103	0.004968365654304745
104-105	0.004569445784251078
106-107	0.007035495890037375
108-109	0.006020063493537136
110-111	0.007325619431894586
112-113	0.004859569326108291
114-115	0.002067130235732631
116-117	0.002502315548518448
118-119	0.15978554067785916
120-121	0.010662040163252517
122-123	0.0017044758084111167
124-125	0.001124228724696694
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1378723.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.51944260616244
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.69465696961143	55.00802488126357
2	9.9492325851785	13.236348118502846
3	2.971415701873296	5.9297074871943165
4	1.3683496395250678	3.640874212462033
5	0.7764233372392725	2.5823623809786445
6	0.4837433883413146	1.9307004334128373
7	0.34451347914853064	1.6041791222289017
8	0.24518611975275084	1.3047715216576625
9	0.18540976280998625	1.1100018668274962
>10	0.9309754488362944	10.718728701583137
>50	0.039617583692994104	1.7414587027680883
>100	0.0102577425582466	1.094124941757521
>500	2.182414323834692E-4	0.09871762936288825
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.253088546430283E-5	0.0
3	0.0	0.0	0.0	7.253088546430283E-5	0.0
4	0.0	0.0	0.0	7.253088546430283E-5	0.0
5	0.0	0.0	0.0	7.253088546430283E-5	0.0
6	0.0	0.0	0.0	7.253088546430283E-5	0.0
7	0.0	0.0	0.0	7.253088546430283E-5	0.0
8	0.0	0.0	0.0	7.253088546430283E-5	7.253088546430283E-5
9	0.0	0.0	0.0	7.253088546430283E-5	1.4506177092860566E-4
10-11	0.0	0.0	3.6265442732151415E-5	7.253088546430283E-5	1.4506177092860566E-4
12-13	0.0	0.0	7.253088546430283E-5	7.253088546430283E-5	2.1759265639290852E-4
14-15	0.0	0.0	7.253088546430283E-5	7.253088546430283E-5	2.538580991250599E-4
16-17	0.0	0.0	7.253088546430283E-5	7.253088546430283E-5	2.901235418572113E-4
18-19	0.0	0.0	7.253088546430283E-5	7.253088546430283E-5	2.901235418572113E-4
20-21	0.0	0.0	7.253088546430283E-5	7.253088546430283E-5	2.901235418572113E-4
22-23	0.0	0.0	7.253088546430283E-5	1.0879632819645425E-4	3.263889845893628E-4
24-25	0.0	0.0	7.253088546430283E-5	2.1759265639290852E-4	4.3518531278581704E-4
26-27	0.0	0.0	7.253088546430283E-5	5.077161982501198E-4	5.802470837144226E-4
28-29	0.0	0.0	7.253088546430283E-5	0.0030462971895007193	5.802470837144226E-4
30-31	0.0	0.0	7.253088546430283E-5	0.012837966727181602	5.802470837144226E-4
32-33	0.0	0.0	7.253088546430283E-5	0.030172848353149984	5.802470837144226E-4
34-35	0.0	0.0	7.253088546430283E-5	0.051641990450583625	5.802470837144226E-4
36-37	0.0	0.0	7.253088546430283E-5	0.08192363513193004	5.802470837144226E-4
38-39	0.0	0.0	7.253088546430283E-5	0.13483491607813897	5.802470837144226E-4
40-41	0.0	0.0	7.253088546430283E-5	0.20120067627797605	6.527779691787256E-4
42-43	0.0	0.0	7.253088546430283E-5	0.2616189038697403	6.527779691787256E-4
44-45	0.0	0.0	7.253088546430283E-5	0.3233789528425942	6.527779691787256E-4
46-47	0.0	0.0	7.253088546430283E-5	0.38909193507325257	6.527779691787256E-4
48-49	0.0	0.0	7.253088546430283E-5	0.4635811544450916	7.253088546430284E-4
50-51	0.0	0.0	7.253088546430283E-5	0.5433288630130926	7.253088546430284E-4
52-53	0.0	0.0	7.253088546430283E-5	0.6296043512728808	7.253088546430284E-4
54-55	0.0	0.0	7.253088546430283E-5	0.7235318479491529	7.253088546430284E-4
56-57	0.0	0.0	7.253088546430283E-5	0.8142317202222636	7.253088546430284E-4
58-59	0.0	0.0	7.253088546430283E-5	0.9262194073791472	7.253088546430284E-4
60-61	0.0	0.0	7.253088546430283E-5	1.0472734552190686	7.253088546430284E-4
62-63	0.0	0.0	7.253088546430283E-5	1.1691978736845616	7.253088546430284E-4
64-65	0.0	0.0	7.253088546430283E-5	1.2847758396719282	7.615742973751797E-4
66-67	0.0	0.0	7.253088546430283E-5	1.4186315887962992	7.978397401073312E-4
68-69	0.0	0.0	7.253088546430283E-5	1.5722520042096926	7.978397401073312E-4
70-71	0.0	0.0	7.253088546430283E-5	1.7424457269516793	0.0010154323965002397
72-73	0.0	0.0	7.253088546430283E-5	1.9041170706516102	0.0011967596101609967
74-75	0.0	0.0	7.253088546430283E-5	2.0667675813053092	0.0012330250528931482
76-77	0.0	0.0	7.253088546430283E-5	2.2553478835124965	0.0012692904956252997
78-79	0.0	0.0	7.253088546430283E-5	2.4713448604251904	0.0013418213810896026
80-81	0.0	0.0	7.253088546430283E-5	2.686471466712313	0.0014506177092860567
82-83	0.0	0.0	7.253088546430283E-5	2.912622767590009	0.0016682103656789652
84-85	0.0	0.0	7.253088546430283E-5	3.139716969978741	0.0016682103656789652
86-87	0.0	0.0	7.253088546430283E-5	3.393502538218337	0.0016682103656789652
88-89	0.0	0.0	7.253088546430283E-5	3.6937078731550863	0.0016682103656789652
90-91	0.0	0.0	7.253088546430283E-5	4.011937133129715	0.0016682103656789652
92-93	0.0	0.0	7.253088546430283E-5	4.325560681877361	0.0016682103656789652
94-95	0.0	0.0	7.253088546430283E-5	4.635630217237255	0.0019583339075361765
96-97	0.0	0.0	7.253088546430283E-5	4.960350991460938	0.002067130235732631
98-99	0.0	0.0	7.253088546430283E-5	5.340594158507546	0.002175926563929085
100-101	0.0	0.0	7.253088546430283E-5	5.744772517757374	0.002175926563929085
102-103	0.0	0.0	7.253088546430283E-5	6.171399186058403	0.002175926563929085
104-105	0.0	0.0	7.253088546430283E-5	6.609558265148257	0.0022121920066612366
106-107	0.0	0.0	7.253088546430283E-5	7.0765121057674385	0.0022847228921255396
108-109	0.0	0.0	7.253088546430283E-5	7.572986016770591	0.002357253777589842
110-111	0.0	0.0	7.253088546430283E-5	8.111564106785771	0.0024660501057862964
112-113	0.0	0.0	7.253088546430283E-5	8.64774867758063	0.0025385809912505993
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1165	0.0	55.161907	1
GTATCAA	2005	0.0	49.561394	1
GTACATG	6925	0.0	41.845684	1
TCAACGC	2295	0.0	40.445255	4
TACATGG	7105	0.0	40.11411	2
CAACGCA	2365	0.0	39.246723	5
ACATGGG	7205	0.0	39.22702	3
AACGCAG	2465	0.0	38.137314	6
ATCAACG	2550	0.0	36.86741	3
CATGGGG	6445	0.0	36.005375	4
TATCAAC	2625	0.0	35.587383	2
ATGGGGG	3795	0.0	34.021847	5
GAGTACA	4700	0.0	28.612253	1
ACGCAGA	3335	0.0	28.185383	7
AGTACAT	4635	0.0	27.985458	2
CGCAGAG	3545	0.0	26.515726	8
TGGGGGG	2825	0.0	25.69516	6
TACCGAC	145	1.3414027E-4	24.61761	7
AGAGTAC	3570	0.0	23.999613	5
ATGGGGA	2625	0.0	22.666302	5
>>END_MODULE