FastQCFastQC Report
Mon 15 Aug 2016
SRR2921290_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921290_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378723
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23290.1689244322463613No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17000.12330250528931481No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13950.10118058522270246No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18050.070.493061
GTATCAA31900.048.6885221
GTACATG65800.039.4010471
TACATGG67150.037.979722
ACATGGG69400.036.3190883
TCAACGC41100.035.3754544
TATCAAC43550.034.7535822
CAACGCA41450.034.6454735
ATCAACG42550.034.4500273
AACGCAG42900.033.6121526
CATGGGG62000.033.541914
ATGGGGG37750.033.1479265
GTGGTAT9200.032.4657971
TGGTATC9500.028.852822
ACGCAGA53750.026.3780167
GGACCGT3450.025.9065996
CGCAGAG54750.025.783678
TGGGGGG28700.024.7060496
GAGTACA45950.024.180841
AGTACAT45050.023.8084832
Produced by FastQC (version 0.11.5)