##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921290_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1378723
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.300623112837023	33.0	30.0	34.0	19.0	34.0
2	30.065429386468494	33.0	31.0	34.0	26.0	34.0
3	30.297618158252238	33.0	31.0	34.0	27.0	34.0
4	32.47553496967846	37.0	35.0	37.0	19.0	37.0
5	32.10324263829645	37.0	33.0	37.0	19.0	37.0
6	32.244257911125004	37.0	35.0	37.0	17.0	37.0
7	32.46602036812325	37.0	35.0	37.0	17.0	37.0
8	32.717740256744825	37.0	35.0	37.0	17.0	37.0
9	34.4768216675866	39.0	37.0	39.0	17.0	39.0
10-11	34.65092407974626	39.0	37.0	39.0	24.0	39.0
12-13	34.68244056275263	39.0	37.0	39.0	25.0	39.0
14-15	36.11244970889729	41.0	38.0	41.0	26.5	41.0
16-17	36.13937027234623	41.0	38.0	41.0	27.0	41.0
18-19	36.16888852945806	41.0	38.0	41.0	27.0	41.0
20-21	36.16485907611609	41.0	38.0	41.0	27.0	41.0
22-23	36.12236867013896	41.0	38.0	41.0	26.0	41.0
24-25	36.073438609495895	41.0	38.0	41.0	25.5	41.0
26-27	35.98125729388717	41.0	38.0	41.0	25.0	41.0
28-29	35.9009670542959	40.0	38.0	41.0	24.5	41.0
30-31	35.80313993456264	40.0	37.5	41.0	24.0	41.0
32-33	35.67038085242648	40.0	37.0	41.0	24.0	41.0
34-35	35.588116322132876	40.0	37.0	41.0	23.0	41.0
36-37	35.506039284178186	40.0	37.0	41.0	22.0	41.0
38-39	35.412711255270274	40.0	36.0	41.0	21.0	41.0
40-41	35.28267316930231	40.0	36.0	41.0	18.5	41.0
42-43	35.13978768759207	40.0	36.0	41.0	15.5	41.0
44-45	34.98239820471552	40.0	35.0	41.0	13.5	41.0
46-47	34.75725834703563	40.0	35.0	41.0	8.5	41.0
48-49	34.53546687768319	40.0	35.0	41.0	4.0	41.0
50-51	34.34910964711548	40.0	35.0	41.0	2.0	41.0
52-53	34.170407688854105	39.5	35.0	41.0	2.0	41.0
54-55	33.97683399783713	39.0	35.0	41.0	2.0	41.0
56-57	33.73781027806166	39.0	35.0	41.0	2.0	41.0
58-59	33.678612745272254	39.0	35.0	41.0	2.0	41.0
60-61	33.449385046887585	38.0	35.0	41.0	2.0	41.0
62-63	33.17335171749511	37.5	34.0	40.5	2.0	41.0
64-65	32.88078207152561	37.0	34.0	40.0	2.0	41.0
66-67	32.56509574439536	36.5	34.0	39.5	2.0	41.0
68-69	32.23625267729631	36.0	34.0	39.0	2.0	41.0
70-71	31.902712147400166	35.5	34.0	39.0	2.0	41.0
72-73	31.55621615074239	35.0	33.0	37.5	2.0	40.0
74-75	31.21950275726161	35.0	33.0	37.0	2.0	39.0
76-77	30.913908377534863	35.0	33.0	36.5	2.0	39.0
78-79	30.64946512098514	35.0	33.0	36.0	2.0	38.0
80-81	30.413229851101345	35.0	33.0	36.0	2.0	37.0
82-83	30.19979466506325	35.0	33.0	35.0	2.0	37.0
84-85	30.016122165221006	35.0	32.0	35.0	2.0	36.5
86-87	29.863856264093656	35.0	32.0	35.0	2.0	36.0
88-89	29.73431972919869	35.0	32.0	35.0	2.0	36.0
90-91	29.63100020816364	35.0	32.0	35.0	2.0	36.0
92-93	29.55404276275945	35.0	32.0	35.0	2.0	35.0
94-95	29.48243809670253	35.0	32.0	35.0	2.0	35.0
96-97	29.42087097988501	35.0	32.0	35.0	2.0	35.0
98-99	29.362485067703954	35.0	32.0	35.0	2.0	35.0
100-101	29.308328794108753	35.0	32.0	35.0	2.0	35.0
102-103	28.646236408618698	34.0	30.0	35.0	2.0	35.0
104-105	29.179727182327415	35.0	31.5	35.0	2.0	35.0
106-107	29.23513715227787	35.0	32.0	35.0	2.0	35.0
108-109	29.301654139373902	35.0	32.0	35.0	2.0	35.0
110-111	29.404913097119582	35.0	32.0	35.0	2.0	35.0
112-113	29.368779660598975	35.0	32.0	35.0	2.0	35.0
114-115	29.305149402744423	35.0	32.0	35.0	2.0	35.0
116-117	29.24736295833173	35.0	32.0	35.0	2.0	35.0
118-119	29.17319069892937	35.0	32.0	35.0	2.0	35.0
120-121	29.077699799016916	35.0	32.0	35.0	2.0	35.0
122-123	28.96618755181425	35.0	32.0	35.0	2.0	35.0
124-125	28.4552919621998	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91475.0
3	5340.0
4	3991.0
5	3837.0
6	4001.0
7	4389.0
8	4509.0
9	4601.0
10	4638.0
11	4858.0
12	5519.0
13	7088.0
14	8616.0
15	7561.0
16	4835.0
17	3946.0
18	3512.0
19	3249.0
20	3017.0
21	3103.0
22	3539.0
23	4366.0
24	5436.0
25	7470.0
26	8619.0
27	9927.0
28	11708.0
29	14108.0
30	17236.0
31	21247.0
32	27189.0
33	36615.0
34	55399.0
35	94516.0
36	213359.0
37	477427.0
38	187950.0
39	527.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.37741957318711	17.481336670667293	11.100759779642797	27.0404839765028
2	16.180407522033068	19.471206326433954	37.25541678785369	27.092969363679288
3	19.80542864665346	22.480875418775202	28.42064722210335	29.293048712467986
4	13.271012714886071	15.110525273438604	31.96647280340495	39.65198920827037
5	13.721590620090712	37.67222925437685	32.68442935639557	15.921750769136867
6	34.65009640426951	33.5811229047401	16.15941645120536	15.609364239785029
7	30.22270168624142	32.06093191264308	18.2062274057216	19.510138995393906
8	28.49659112422902	34.74101030968216	17.574769314242566	19.187629251846246
9	27.741848441063315	15.484262660376375	17.73983194935269	39.03405694920762
10-11	27.156105173332328	25.190515237048356	25.368509580251768	22.28487000936755
12-13	26.371597155622517	22.39335852299055	26.41405163408013	24.820992687306802
14-15	23.628749212133258	23.969390515716356	23.91553633325911	28.486323938891278
16-17	23.868246195936386	27.194077418016526	24.572666155565695	24.365010230481396
18-19	23.838544798338752	26.184338696025232	26.462277049124445	23.51483945651157
20-21	24.45433201593068	25.161181760223045	26.57462739070865	23.80985883313762
22-23	24.29820928496877	24.704491039897064	26.48671995752591	24.51057971760825
24-25	23.88329635467023	25.02986459208993	26.559686028303002	24.527153024936844
26-27	23.677381170837073	25.29724970135408	26.58579714707015	24.439571980738698
28-29	23.749694463644982	25.787123301779978	26.410562527788393	24.052619706786643
30-31	24.421983241013603	25.322998165693907	26.410562527788393	23.844456065504094
32-33	23.866360392914313	25.398720410118642	26.505940642173954	24.228978554793095
34-35	23.989626632760896	25.576058425078855	26.66351399084515	23.770800951315092
36-37	24.086926815611257	25.49783386510561	26.66518220121083	23.750057118072306
38-39	24.00032493836688	25.72442035129609	26.543547906287195	23.731706804049836
40-41	23.934974610563543	25.670711230609772	26.498723819070257	23.895590339756424
42-43	23.793793242007276	25.6596865360192	26.250378067240483	24.29614215473304
44-45	23.559953667270364	25.772689655572584	26.58376628227715	24.0835903948799
46-47	23.65239428079462	25.688843951975848	26.156015385251425	24.502746381978106
48-49	23.691452162617146	26.001814722754318	25.963663477000093	24.343069637628442
50-51	23.77207024181072	26.067128785114924	26.058461344301936	24.10233962877242
52-53	23.954412887867978	25.981361013053384	25.98991965753817	24.074306441540468
54-55	23.577651203323654	25.671218946808022	26.30691589245991	24.444213957408415
56-57	23.654135022045764	25.943681218054675	25.88522132437045	24.51696243552911
58-59	23.717925935811618	26.027454390765946	26.083194376245267	24.17142529717717
60-61	24.16808887644581	25.735626373100324	26.201999966635793	23.89428478381807
62-63	23.84456486183229	25.92906624463362	26.180603355423827	24.045765538110267
64-65	23.679665893729197	25.604744390279993	26.194239161891115	24.5213505540997
66-67	23.5834536741608	25.957208228193775	26.024371828133713	24.434966269511715
68-69	23.485573244226725	25.75227221131438	26.220966793184708	24.541187751274187
70-71	23.436215976668265	25.713649514804644	26.317287809081304	24.53284669944579
72-73	23.610217570897127	26.077464436293585	25.88489493538586	24.42742305742343
74-75	23.42852770280905	26.081272307780463	26.047654242367756	24.442545747042736
76-77	23.450540826547464	25.62236939544782	26.10118203584041	24.825907742164308
78-79	23.332968261209828	25.634627115091284	26.36548458247233	24.666920041226557
80-81	23.568403515426954	25.86288181164744	25.930154207915585	24.638560465010013
82-83	23.611704454049146	26.01686488148816	25.97494202968979	24.396488634772904
84-85	23.64434335250808	26.042540814942523	25.765799221453477	24.54731661109592
86-87	23.264861759758848	26.160367238379283	25.936391864065516	24.638379137796353
88-89	23.12716187370487	25.899836297791506	26.059948227453955	24.91305360104967
90-91	23.418906573952754	25.62254233360399	26.095045301027074	24.863505791416177
92-93	23.443229952419635	25.81669954740629	26.103596089126146	24.636474411047928
94-95	23.43158249756568	25.757467510684712	25.954171343399413	24.856778648350193
96-97	23.093421105598345	25.68709588861259	26.089897183582302	25.129585822206764
98-99	23.051955705372183	25.66179923806277	25.996746988607207	25.289498067957837
100-101	23.058547656055637	25.813125624218934	26.107347161104876	25.02097955862055
102-103	22.91301443437152	25.720793807022872	26.151989921108154	25.214201837497452
104-105	23.036715859530883	25.78327916485037	25.963409618900968	25.216595356717775
106-107	23.098855316735353	25.587718191194455	26.113966412373195	25.199460079696994
108-109	22.974252558261487	25.54413715045011	26.113832436547103	25.367777854741302
110-111	22.866680689335038	25.952260067308806	26.035852094696533	25.145207148659626
112-113	22.858567579231387	25.965010897828776	26.035221202351465	25.141200320588375
114-115	22.859274232884765	25.986956747215988	25.94071822388947	25.213050796009778
116-117	22.554801835764035	25.798649832180992	26.157715413055975	25.488832918998998
118-119	22.705346946428268	25.686659706111787	26.118871722824373	25.489121624635576
120-121	22.554526985517736	25.815592857438162	26.220352050922557	25.40952810612154
122-123	22.526835881075314	25.897717748799685	25.950194548578033	25.625251821546968
124-125	22.335245735174656	25.800089938493677	25.91175147963328	25.95291284669839
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	32.0
1	32.0
2	52.0
3	71.0
4	67.0
5	86.5
6	211.5
7	454.5
8	648.0
9	791.0
10	776.5
11	650.0
12	655.0
13	713.0
14	790.5
15	844.5
16	894.5
17	974.5
18	1042.0
19	1103.5
20	1196.5
21	1328.5
22	1440.0
23	1649.5
24	1947.0
25	2249.5
26	2769.0
27	3660.0
28	4532.0
29	5543.0
30	7011.0
31	8659.0
32	10688.5
33	12920.5
34	15222.5
35	17638.0
36	20393.5
37	23441.0
38	26017.5
39	28552.5
40	31549.0
41	34151.0
42	36634.5
43	40225.5
44	44123.5
45	47882.5
46	51074.5
47	54152.0
48	58156.0
49	61249.5
50	63183.5
51	63891.5
52	63070.5
53	61939.5
54	60547.5
55	57804.0
56	53587.0
57	48835.0
58	44521.0
59	39775.5
60	33757.0
61	27791.0
62	22907.0
63	17892.5
64	12974.5
65	9525.5
66	6965.5
67	5248.0
68	4003.5
69	2987.0
70	2262.5
71	1718.5
72	1324.5
73	1043.5
74	797.5
75	622.5
76	480.0
77	322.5
78	237.0
79	194.0
80	156.0
81	127.5
82	121.5
83	127.0
84	122.5
85	111.0
86	93.5
87	86.5
88	81.0
89	68.5
90	67.0
91	62.0
92	67.0
93	74.5
94	63.0
95	49.0
96	38.0
97	34.0
98	23.0
99	8.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3461173854356531
2	0.0
3	0.0
4	0.018858030220718736
5	0.1816898680880786
6	0.04874075503201151
7	0.024080253974148542
8	0.008413582713859129
9	0.006600310577251558
10-11	0.001994599350268328
12-13	0.056320232563031154
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	1.0879632819645426E-4
92-93	2.1759265639290852E-4
94-95	3.6265442732151415E-5
96-97	2.538580991250599E-4
98-99	3.6265442732151415E-5
100-101	0.0
102-103	0.0
104-105	0.0
106-107	7.253088546430283E-5
108-109	2.538580991250599E-4
110-111	2.1759265639290852E-4
112-113	5.802470837144226E-4
114-115	1.813272136607571E-4
116-117	3.9891987005366564E-4
118-119	1.0879632819645426E-4
120-121	1.4506177092860566E-4
122-123	0.0013418213810896026
124-125	2.1759265639290852E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1378723.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.69421452502156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.31221054425414	57.074488676615246
2	8.884944476790244	12.029186749094885
3	2.739061484374349	5.562558471613836
4	1.2653954620119467	3.4263980745770204
5	0.6777399201000078	2.293953577171045
6	0.4496273031048841	1.826230026761335
7	0.3198749644471166	1.515757912512673
8	0.24061341988107152	1.3030509170422675
9	0.1705787027052663	1.0392472164897155
>10	0.8890167490536951	10.451464482860471
>50	0.03916408909587536	1.7651070684525334
>100	0.011023727347628593	1.1313791608430808
>500	4.2808961929293674E-4	0.18821984245565893
>1k	3.210672144697026E-4	0.3929578235101978
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2329	0.1689244322463613	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1700	0.12330250528931481	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1395	0.10118058522270246	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	2.901235418572113E-4
7	0.0	0.0	0.0	0.0	2.901235418572113E-4
8	0.0	0.0	0.0	0.0	2.901235418572113E-4
9	0.0	0.0	0.0	7.253088546430283E-5	2.901235418572113E-4
10-11	0.0	0.0	0.0	7.253088546430283E-5	2.901235418572113E-4
12-13	0.0	0.0	0.0	1.4506177092860566E-4	2.901235418572113E-4
14-15	0.0	0.0	0.0	1.4506177092860566E-4	3.626544273215142E-4
16-17	0.0	0.0	0.0	1.4506177092860566E-4	4.3518531278581704E-4
18-19	0.0	0.0	0.0	1.4506177092860566E-4	5.077161982501198E-4
20-21	0.0	0.0	0.0	1.4506177092860566E-4	5.077161982501198E-4
22-23	0.0	0.0	0.0	1.813272136607571E-4	5.439816409822712E-4
24-25	0.0	0.0	0.0	2.901235418572113E-4	5.802470837144226E-4
26-27	0.0	0.0	0.0	5.077161982501198E-4	7.253088546430284E-4
28-29	0.0	0.0	0.0	0.003010031746768568	7.253088546430284E-4
30-31	0.0	0.0	0.0	0.012257719643467179	7.253088546430284E-4
32-33	0.0	7.253088546430283E-5	0.0	0.02886729241479253	8.703706255716341E-4
34-35	0.0	7.253088546430283E-5	0.0	0.04953859477211883	8.703706255716341E-4
36-37	0.0	7.253088546430283E-5	0.0	0.07873227617150072	8.703706255716341E-4
38-39	0.0	7.253088546430283E-5	0.0	0.12946763055378055	8.703706255716341E-4
40-41	0.0	7.253088546430283E-5	0.0	0.19246070457952758	8.703706255716341E-4
42-43	0.0	7.253088546430283E-5	0.0	0.2491798570126124	8.703706255716341E-4
44-45	0.0	7.253088546430283E-5	0.0	0.30629792931575084	8.703706255716341E-4
46-47	0.0	7.253088546430283E-5	0.0	0.36820304005953336	8.703706255716341E-4
48-49	0.0	7.253088546430283E-5	0.0	0.43863052984537143	8.703706255716341E-4
50-51	0.0	7.253088546430283E-5	0.0	0.5128296256753532	8.703706255716341E-4
52-53	0.0	7.253088546430283E-5	0.0	0.5940279519526402	8.703706255716341E-4
54-55	0.0	7.253088546430283E-5	0.0	0.6820441814635717	8.703706255716341E-4
56-57	0.0	7.253088546430283E-5	0.0	0.7682471388378956	8.703706255716341E-4
58-59	0.0	7.253088546430283E-5	0.0	0.8740334352875814	9.429015110359369E-4
60-61	0.0	7.253088546430283E-5	0.0	0.988450907107519	0.0010516978392323911
62-63	0.0	7.253088546430283E-5	0.0	1.104572854735868	0.0011604941674288453
64-65	0.0	7.253088546430283E-5	0.0	1.2130427939477328	0.0011604941674288453
66-67	0.0	7.253088546430283E-5	0.0	1.338593756686441	0.0011967596101609967
68-69	0.0	7.253088546430283E-5	0.0	1.4830390150886	0.0012330250528931482
70-71	0.0	7.253088546430283E-5	0.0	1.6440575808193523	0.0012330250528931482
72-73	0.0	7.253088546430283E-5	0.0	1.7938338593031378	0.0013780868238217538
74-75	0.0	7.253088546430283E-5	0.0	1.9465113732054953	0.0013780868238217538
76-77	0.0	7.253088546430283E-5	0.0	2.1238131227229835	0.0014143522665539053
78-79	0.0	7.253088546430283E-5	0.0	2.3276249108776748	0.0014506177092860567
80-81	0.0	7.253088546430283E-5	0.0	2.528680525384722	0.0015231485947503594
82-83	0.0	7.253088546430283E-5	0.0	2.740434445497754	0.0017407412511432682
84-85	0.0	7.253088546430283E-5	0.0	2.9553797245712152	0.0017407412511432682
86-87	3.6265442732151415E-5	7.253088546430283E-5	0.0	3.1949129738170754	0.0017770066938754196
88-89	7.253088546430283E-5	7.253088546430283E-5	0.0	3.476949321945017	0.0018132721366075708
90-91	7.253088546430283E-5	7.253088546430283E-5	0.0	3.7772634532099634	0.0018132721366075708
92-93	7.253088546430283E-5	7.253088546430283E-5	0.0	4.070795946683997	0.0018132721366075708
94-95	7.253088546430283E-5	1.4506177092860566E-4	0.0	4.36128214296853	0.0018858030220718738
96-97	7.253088546430283E-5	1.4506177092860566E-4	0.0	4.667833930383406	0.0018858030220718738
98-99	7.253088546430283E-5	1.4506177092860566E-4	0.0	5.026390362676187	0.0018858030220718738
100-101	7.253088546430283E-5	1.4506177092860566E-4	0.0	5.407322573134705	0.0018858030220718738
102-103	7.253088546430283E-5	1.4506177092860566E-4	0.0	5.810086580117979	0.0019583339075361765
104-105	7.253088546430283E-5	1.4506177092860566E-4	0.0	6.224129139790952	0.0020308647930004794
106-107	7.253088546430283E-5	1.4506177092860566E-4	0.0	6.667038991878717	0.0020308647930004794
108-109	7.253088546430283E-5	1.4506177092860566E-4	0.0	7.1380545620839	0.0021396611211969337
110-111	7.253088546430283E-5	1.4506177092860566E-4	0.0	7.645589433120358	0.002175926563929085
112-113	7.253088546430283E-5	1.4506177092860566E-4	0.0	8.155554088819873	0.002175926563929085
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1805	0.0	70.49306	1
GTATCAA	3190	0.0	48.688522	1
GTACATG	6580	0.0	39.401047	1
TACATGG	6715	0.0	37.97972	2
ACATGGG	6940	0.0	36.319088	3
TCAACGC	4110	0.0	35.375454	4
TATCAAC	4355	0.0	34.753582	2
CAACGCA	4145	0.0	34.645473	5
ATCAACG	4255	0.0	34.450027	3
AACGCAG	4290	0.0	33.612152	6
CATGGGG	6200	0.0	33.54191	4
ATGGGGG	3775	0.0	33.147926	5
GTGGTAT	920	0.0	32.465797	1
TGGTATC	950	0.0	28.85282	2
ACGCAGA	5375	0.0	26.378016	7
GGACCGT	345	0.0	25.906599	6
CGCAGAG	5475	0.0	25.78367	8
TGGGGGG	2870	0.0	24.706049	6
GAGTACA	4595	0.0	24.18084	1
AGTACAT	4505	0.0	23.808483	2
>>END_MODULE