FastQCFastQC Report
Mon 15 Aug 2016
SRR2921289_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921289_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550362
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13250.055.6897351
GTATCAA22800.049.8503151
TCAACGC26900.040.6985444
GTACATG77900.039.340431
CAACGCA28000.038.4621735
TACATGG81750.036.9733922
AACGCAG29700.036.6589436
ACATGGG81800.036.4416283
ATCAACG30050.036.03633
TATCAAC31650.034.4025542
ATGGGGG47100.033.7290155
CATGGGG76650.033.6892174
TGGGGGG32450.028.235236
ACGCAGA40000.027.0687797
CGCAGAG42600.025.695178
GTATAGG4750.025.0556551
AGTACAT52000.024.6007542
GAGTACA53200.024.3845211
AGAGTAC44550.023.9066455
ACCGTAT1751.7535656E-523.795968
Produced by FastQC (version 0.11.5)