##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921289_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1550362
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.50087656947216	31.0	31.0	34.0	27.0	34.0
2	30.65386148525312	31.0	31.0	34.0	27.0	34.0
3	30.729503174097406	33.0	31.0	34.0	27.0	34.0
4	34.2093265959821	37.0	35.0	37.0	31.0	37.0
5	34.24926630038662	37.0	35.0	37.0	31.0	37.0
6	34.47232323805666	37.0	35.0	37.0	32.0	37.0
7	34.49620346731925	37.0	35.0	37.0	32.0	37.0
8	34.48750678873708	37.0	35.0	37.0	32.0	37.0
9	36.14335619681081	39.0	37.0	39.0	32.0	39.0
10-11	36.1417201272993	39.0	37.0	39.0	32.0	39.0
12-13	36.06702047650807	39.0	37.0	39.0	32.0	39.0
14-15	37.4234101455015	41.0	38.0	41.0	32.0	41.0
16-17	37.41150969902513	41.0	38.0	41.0	32.0	41.0
18-19	37.40191548812471	41.0	38.0	41.0	32.0	41.0
20-21	37.42578088214236	41.0	38.0	41.0	32.0	41.0
22-23	37.3795449062864	41.0	38.0	41.0	31.0	41.0
24-25	37.36611739709823	41.0	38.0	41.0	31.0	41.0
26-27	37.332823559916974	41.0	38.0	41.0	31.0	41.0
28-29	37.26800450475437	41.0	38.0	41.0	31.0	41.0
30-31	37.19775220238886	41.0	38.0	41.0	30.5	41.0
32-33	37.121613210334104	40.5	38.0	41.0	30.0	41.0
34-35	37.02154690323937	40.0	38.0	41.0	30.0	41.0
36-37	36.91766084308052	40.0	38.0	41.0	30.0	41.0
38-39	36.82185966890313	40.0	38.0	41.0	30.0	41.0
40-41	36.721962032093145	40.0	37.5	41.0	30.0	41.0
42-43	36.538918652546954	40.0	37.0	41.0	30.0	41.0
44-45	36.318115382084954	40.0	37.0	41.0	29.0	41.0
46-47	36.11655600433963	40.0	36.0	41.0	28.5	41.0
48-49	35.9145357664855	40.0	36.0	41.0	28.0	41.0
50-51	35.74487216533945	40.0	35.0	41.0	28.0	41.0
52-53	35.546394325970326	39.0	35.0	41.0	27.5	41.0
54-55	35.33450026509938	39.0	35.0	41.0	27.0	41.0
56-57	35.084499297583406	39.0	35.0	41.0	26.5	41.0
58-59	34.81155207622478	38.0	35.0	40.0	26.0	41.0
60-61	34.51864209778103	38.0	34.5	40.0	26.0	41.0
62-63	34.18390575878408	37.0	34.0	40.0	26.0	41.0
64-65	33.81975177410179	36.5	34.0	39.5	25.5	41.0
66-67	33.46837641789466	36.0	34.0	39.0	24.5	41.0
68-69	33.10478133493984	35.5	33.5	39.0	23.5	40.0
70-71	33.028490120371885	35.0	34.0	38.5	25.0	40.0
72-73	32.726909263771944	35.0	34.0	37.0	24.0	39.5
74-75	32.40576297664674	35.0	33.5	37.0	24.0	39.0
76-77	32.11206608521107	35.0	33.0	36.0	23.0	39.0
78-79	31.83281904484243	35.0	33.0	36.0	23.0	37.5
80-81	31.57435360257798	35.0	33.0	35.5	21.5	37.0
82-83	31.36741257848167	35.0	33.0	35.0	20.5	37.0
84-85	31.163761108695905	35.0	33.0	35.0	20.0	36.0
86-87	31.004035186620932	35.0	33.0	35.0	20.0	36.0
88-89	30.883081499675562	35.0	33.0	35.0	19.0	36.0
90-91	30.771233428063898	35.0	33.0	35.0	18.5	35.0
92-93	30.664823118729693	35.0	32.0	35.0	18.0	35.0
94-95	30.548756677472745	35.0	32.0	35.0	17.0	35.0
96-97	30.46249230824801	35.0	32.0	35.0	15.5	35.0
98-99	30.380208299739028	35.0	32.0	35.0	12.5	35.0
100-101	30.293459527516802	35.0	32.0	35.0	9.0	35.0
102-103	29.47375322666577	34.0	30.5	35.0	7.0	35.0
104-105	30.05789389832826	34.5	31.5	35.0	7.0	35.0
106-107	30.12974614960893	35.0	32.0	35.0	6.0	35.0
108-109	30.058298320005264	35.0	32.0	35.0	2.0	35.0
110-111	29.97740301942385	35.0	32.0	35.0	2.0	35.0
112-113	29.887024449773666	35.0	31.5	35.0	2.0	35.0
114-115	29.75702352095833	35.0	31.0	35.0	2.0	35.0
116-117	29.62704194246247	35.0	31.0	35.0	2.0	35.0
118-119	29.62056474552395	34.5	31.5	35.0	2.0	35.0
120-121	29.778963235683023	35.0	32.0	35.0	2.0	35.0
122-123	29.647008569611486	35.0	32.0	35.0	2.0	35.0
124-125	29.090623028686203	34.5	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	45855.0
3	6590.0
4	4276.0
5	3733.0
6	3902.0
7	4469.0
8	4605.0
9	4841.0
10	5193.0
11	5916.0
12	6032.0
13	5979.0
14	5832.0
15	5075.0
16	4100.0
17	3857.0
18	3812.0
19	3609.0
20	3642.0
21	3866.0
22	4514.0
23	5805.0
24	8586.0
25	10889.0
26	11460.0
27	12921.0
28	15539.0
29	18891.0
30	23226.0
31	29173.0
32	37624.0
33	51336.0
34	80526.0
35	137553.0
36	303174.0
37	526005.0
38	137894.0
39	62.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.07018605452292	17.013012398258994	11.403572716476027	25.513228830742058
2	17.133934682466112	18.72336198160086	38.26720879690823	25.875494539024796
3	21.051249747612072	22.295610933606334	27.840275372163337	28.81286394661826
4	13.937212325298601	14.147192681974913	33.773098645610574	38.142496347115916
5	14.921979319197796	37.40612683279255	32.599356224560225	15.072537623449422
6	35.86100147946269	32.722062963299905	16.37725847110144	15.039677086135958
7	31.30952987645847	31.216894695861804	18.527939247835878	18.94563617984385
8	28.51055923940705	34.731082424448076	18.152137941978854	18.606220394166016
9	28.17324699753455	15.664103169473353	17.570147836508998	38.592501996483094
10-11	27.507570599529558	24.880687867463145	25.691981340959675	21.919760192047622
12-13	26.815882641507855	22.00533007882234	26.31348255790889	24.86530472176092
14-15	24.28360866171699	23.573700065149943	23.1838002412483	28.95889103188477
16-17	24.995371848583655	26.779322645044974	23.975856208012022	24.249449298359345
18-19	24.9047425840481	26.169423062905693	25.344407081849457	23.581427271196752
20-21	25.494933987837697	25.143578670339117	25.246301883688943	24.115185458134246
22-23	25.406490823276556	24.664657568531123	25.142022639766598	24.786828968425727
24-25	25.001967396689096	24.828449459191678	25.162971401147672	25.006611742971558
26-27	24.642139419766437	25.233416081319003	25.262668682204193	24.861775816710363
28-29	24.714283548763618	25.77513135619323	25.03596955239073	24.474615542652423
30-31	25.3943317372786	25.17043684338517	25.065520379853716	24.36971103948252
32-33	24.903885849932912	25.145558107131798	25.133366446485706	24.81718959644958
34-35	24.918802947412694	25.373635454100363	25.310193705365812	24.39736789312113
36-37	24.900129204716908	25.459392757182215	25.198306340964994	24.442171697135887
38-39	24.985236304596242	25.577235494407212	25.131179989363044	24.3063482116335
40-41	25.035107263413813	25.232011115633686	25.22465745825348	24.508224162699026
42-43	24.8367279997652	25.220182157657746	25.08930008408318	24.853789758493868
44-45	24.42895519143519	25.330067624554868	25.444694260701738	24.7962829233082
46-47	24.6163204630054	25.1771765224215	25.280129684860757	24.92637332971234
48-49	24.482571218926132	25.50209242132254	25.22987606737677	24.785460292374555
50-51	24.56976140016584	25.495178753942447	25.370684592105846	24.564375253785865
52-53	24.68170307079615	25.42685782328634	25.23876301214876	24.652676093768747
54-55	24.357820712289747	25.05112019341172	25.627923688033295	24.963135406265238
56-57	24.556587647153684	25.32559264634736	25.197419771004675	24.920399935494274
58-59	24.5769635294425	25.444178215069112	25.373447710547424	24.605410544940963
60-61	24.929948228773863	25.320973861529712	25.437630056983178	24.311447852713247
62-63	24.81367833856681	25.44134076770846	25.37819072216818	24.36679017155655
64-65	24.571996752906436	25.176811468935718	25.409700450135926	24.84149132802192
66-67	24.577389065234346	25.421707885490775	25.19997703674701	24.800926012527874
68-69	24.410456012065026	25.366646756105084	25.457566636951334	24.765330594878556
70-71	24.39301600734831	25.231569876048837	25.591632006440097	24.783782110162758
72-73	24.495651952356116	25.637887277473588	25.136917622372795	24.729543147797497
74-75	24.32872799704316	25.661112698614403	25.292571839182404	24.717587465160037
76-77	24.39144509274546	25.236415216106135	25.307659882028638	25.064479809119767
78-79	24.270195760469015	25.280314362325544	25.52787727381503	24.92161260339041
80-81	24.52068565486916	25.61052974124266	25.14736535545875	24.72141924842943
82-83	24.694531086471123	25.52173622773427	25.178959549578284	24.604773136216316
84-85	24.6285113263285	25.677946066208214	25.02213401943149	24.67140858803179
86-87	24.25380258795299	25.80741295008579	25.247571375124174	24.691213086837045
88-89	24.199181055390998	25.434370903986874	25.328971133105593	25.037476907516538
90-91	24.62170653193011	25.26849104148303	25.383727954449654	24.72607447213721
92-93	24.51251981544823	25.575547051509684	25.32794062864358	24.583992504398505
94-95	24.655210727759275	25.29823252057641	25.23704917599226	24.809507575672058
96-97	24.29765079414227	25.32317868491433	25.38326669788792	24.995903823055478
98-99	24.1750316802413	25.42343799235804	25.259529632676912	25.14200069472374
100-101	24.20313797180689	25.48613534575681	25.404792944562672	24.905933737873628
102-103	24.07891609222724	25.401709820712977	25.513528366981568	25.005845720078216
104-105	24.267788094515495	25.493110785077977	25.32620604398351	24.91289507642302
106-107	24.293461871533832	25.300588129998175	25.4686575251451	24.937292473322895
108-109	24.260836527408845	25.342425106401095	25.458824485604403	24.937913880585654
110-111	24.08280744939355	25.786277928613956	25.367473699352427	24.763440922640072
112-113	24.103155392646176	25.87141375846015	25.3973066199225	24.62812422897117
114-115	24.225881305724112	25.904687880463968	25.222122422520403	24.647308391291517
116-117	24.012558697559214	25.841987460653282	25.32022421177563	24.82522963001187
118-119	24.128450175765117	25.672560388876487	25.35610888360806	24.842880551750333
120-121	23.892999820023572	25.730855894357063	25.574102230482072	24.802042055137292
122-123	23.970375425432383	25.94823891231215	25.30379734179946	24.777588320456005
124-125	23.966048715796997	25.86469797895815	25.28846916706604	24.88078413817881
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	239.0
1	229.0
2	202.0
3	168.5
4	152.0
5	156.0
6	148.5
7	175.0
8	229.5
9	252.0
10	245.5
11	238.5
12	264.0
13	293.5
14	327.5
15	365.0
16	387.5
17	424.5
18	451.0
19	470.5
20	517.5
21	577.0
22	677.0
23	808.5
24	944.5
25	1158.5
26	1545.0
27	2048.0
28	2652.0
29	3518.0
30	4802.5
31	6336.5
32	8377.0
33	10608.5
34	12943.0
35	15635.5
36	18750.5
37	22176.0
38	25369.5
39	28642.0
40	32044.0
41	35311.0
42	38788.5
43	43358.0
44	48727.5
45	53808.5
46	57796.5
47	61629.0
48	66644.0
49	71170.0
50	73161.5
51	74509.5
52	74839.0
53	73766.0
54	73533.5
55	71620.5
56	66697.0
57	60810.0
58	55268.5
59	49244.5
60	42184.0
61	35462.5
62	29270.0
63	22595.5
64	16576.5
65	12342.0
66	9233.0
67	6913.0
68	5162.5
69	3833.0
70	2847.0
71	2196.5
72	1621.5
73	1149.5
74	868.5
75	640.0
76	446.0
77	270.5
78	165.0
79	114.0
80	76.5
81	56.0
82	46.5
83	38.0
84	28.0
85	20.5
86	17.0
87	13.0
88	12.5
89	10.5
90	6.0
91	5.5
92	4.0
93	5.0
94	5.5
95	2.5
96	0.5
97	1.0
98	1.5
99	3.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014512739605330885
2	0.011674692749177288
3	0.010900679970226309
4	0.01399673108603023
5	0.008514140568460785
6	0.03063800583347631
7	0.012577707657953434
8	0.013093716177254086
9	0.008772144828111112
10-11	0.007836879386878676
12-13	0.0066758602184522065
14-15	0.005934097971957517
16-17	0.004321571349142974
18-19	0.005740594777219772
20-21	0.005127834660550246
22-23	0.005224586257919118
24-25	0.005805095842132353
26-27	0.004676327206162174
28-29	0.005966348504413808
30-31	0.005224586257919118
32-33	0.007869129919334968
34-35	0.007804628854422386
36-37	0.007030616075471407
38-39	0.006869363413189951
40-41	0.0072886203351217324
42-43	0.00719186873775286
44-45	0.00719186873775286
46-47	0.009191401750042894
48-49	0.008030382581616423
50-51	0.005708344244763481
52-53	0.005160085193006537
54-55	0.0060631001017826805
56-57	0.007224119270209151
58-59	0.00719186873775286
60-61	0.005160085193006537
62-63	0.005547091582482027
64-65	0.0033863059079105393
66-67	0.0038055628298423205
68-69	0.006192102231607844
70-71	0.0047730788035310456
72-73	0.004418322946511847
74-75	0.0038055628298423205
76-77	0.00454732507633701
78-79	0.0031605521807165035
80-81	0.003128301648260213
82-83	0.005256836790375409
84-85	0.00944940600969322
86-87	0.006579108621083335
88-89	0.0047730788035310456
90-91	0.004998832530725083
92-93	0.007707877257053514
94-95	0.0066758602184522065
96-97	0.008772144828111112
98-99	0.0068048623482773705
100-101	0.008062633114072713
102-103	0.00532133785528799
104-105	0.004934331465812501
106-107	0.007062866607927697
108-109	0.006192102231607844
110-111	0.0074176224649468965
112-113	0.005127834660550246
114-115	0.002225286739484069
116-117	0.0027090447263284317
118-119	0.1558023222963411
120-121	0.010255669321100492
122-123	0.0017737792850959968
124-125	0.0014835244929893793
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1550362.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.813859518003554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.66512473974177	47.65078579667144
2	10.745235749066344	12.85428043164968
3	3.617573394793246	6.491430804966912
4	1.7881237325695778	4.278183269628995
5	1.0316206086155726	3.0852605079804594
6	0.6704079754346635	2.4059813077438883
7	0.4655407350148455	1.949205168686026
8	0.3364069183800943	1.6097436925497157
9	0.2549644813194236	1.3725368700948581
>10	1.3478548965644492	14.113270652064228
>50	0.06108519350724244	2.4358682528043114
>100	0.015846001434508497	1.6274760657458969
>500	1.0778677914694353E-4	0.060216318102509936
>1k	1.0778677914694353E-4	0.06576086131109223
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.450106491258171E-5
2	0.0	0.0	0.0	0.0	6.450106491258171E-5
3	0.0	0.0	0.0	0.0	6.450106491258171E-5
4	0.0	0.0	0.0	0.0	6.450106491258171E-5
5	0.0	0.0	0.0	0.0	6.450106491258171E-5
6	0.0	0.0	0.0	0.0	3.8700638947549023E-4
7	0.0	0.0	0.0	0.0	3.8700638947549023E-4
8	0.0	0.0	0.0	0.0	3.8700638947549023E-4
9	0.0	0.0	0.0	0.0	3.8700638947549023E-4
10-11	0.0	0.0	0.0	0.0	3.8700638947549023E-4
12-13	0.0	0.0	0.0	0.0	3.8700638947549023E-4
14-15	0.0	0.0	0.0	0.0	4.837579868443628E-4
16-17	0.0	0.0	0.0	0.0	5.160085193006537E-4
18-19	0.0	0.0	0.0	0.0	5.160085193006537E-4
20-21	0.0	0.0	0.0	6.450106491258171E-5	5.160085193006537E-4
22-23	0.0	0.0	0.0	9.675159736887256E-5	5.160085193006537E-4
24-25	0.0	0.0	0.0	1.6125266228145425E-4	5.160085193006537E-4
26-27	0.0	0.0	0.0	5.482590517569445E-4	5.160085193006537E-4
28-29	0.0	0.0	0.0	0.0034830575052794124	5.160085193006537E-4
30-31	0.0	0.0	0.0	0.013125966709710377	5.160085193006537E-4
32-33	0.0	0.0	0.0	0.031895776599271654	5.160085193006537E-4
34-35	0.0	0.0	0.0	0.05311662695551104	5.805095842132354E-4
36-37	0.0	0.0	0.0	0.08514140568460785	5.805095842132354E-4
38-39	0.0	0.0	0.0	0.1410638289638162	5.805095842132354E-4
40-41	0.0	0.0	0.0	0.21088623173168589	5.805095842132354E-4
42-43	0.0	0.0	0.0	0.2704207146459988	5.805095842132354E-4
44-45	0.0	0.0	0.0	0.33643755458402613	5.805095842132354E-4
46-47	0.0	0.0	0.0	0.4052601908457509	5.805095842132354E-4
48-49	0.0	0.0	0.0	0.48337098045488736	5.805095842132354E-4
50-51	0.0	0.0	0.0	0.5657065898157978	5.805095842132354E-4
52-53	0.0	0.0	0.0	0.6551373163170924	5.805095842132354E-4
54-55	0.0	0.0	0.0	0.7513729051666642	6.127601166695262E-4
56-57	0.0	0.0	0.0	0.8493177722364196	6.45010649125817E-4
58-59	0.0	0.0	0.0	0.9610981177299237	6.772611815821078E-4
60-61	0.0	0.0	0.0	1.08242462083049	7.740127789509805E-4
62-63	0.0	0.0	0.0	1.2122330139670605	7.740127789509805E-4
64-65	0.0	0.0	0.0	1.3338820223921897	7.740127789509805E-4
66-67	0.0	0.0	0.0	1.475848866264782	7.740127789509805E-4
68-69	0.0	0.0	0.0	1.6323607002751617	8.062633114072713E-4
70-71	0.0	0.0	0.0	1.8000312185154177	9.675159736887256E-4
72-73	0.0	0.0	0.0	1.9603486153556395	0.0012255202333390524
74-75	0.0	0.0	0.0	2.124084568636228	0.0012255202333390524
76-77	0.0	0.0	0.0	2.3188132836073123	0.0012255202333390524
78-79	0.0	0.0	0.0	2.537987902180265	0.0012255202333390524
80-81	0.0	0.0	0.0	2.751970185027755	0.0012577707657953432
82-83	0.0	0.0	0.0	2.9815617255840894	0.0013545223631642159
84-85	0.0	0.0	0.0	3.2165391050606242	0.0013867728956205067
86-87	0.0	0.0	0.0	3.4758978870741157	0.0014190234280767975
88-89	0.0	0.0	0.0	3.774860322943932	0.0014190234280767975
90-91	0.0	0.0	0.0	4.087496984575216	0.0014190234280767975
92-93	0.0	0.0	0.0	4.385524155003798	0.0014190234280767975
94-95	0.0	0.0	0.0	4.693419988364008	0.0014512739605330883
96-97	0.0	0.0	0.0	5.034082362699808	0.0014835244929893793
98-99	0.0	0.0	0.0	5.42924813688674	0.001548025557901961
100-101	0.0	0.0	0.0	5.850762596090462	0.001548025557901961
102-103	0.0	0.0	0.0	6.280178435745974	0.0018382803500085786
104-105	0.0	0.0	0.0	6.730944127887551	0.0019027814149211605
106-107	0.0	0.0	0.0	7.208381010370481	0.0019350319473774513
108-109	0.0	0.0	0.0	7.701588403224537	0.001999533012290033
110-111	0.0	0.0	0.0	8.234883207921762	0.0020640340772026147
112-113	0.0	0.0	0.0	8.767145995580385	0.0020640340772026147
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1325	0.0	55.689735	1
GTATCAA	2280	0.0	49.850315	1
TCAACGC	2690	0.0	40.698544	4
GTACATG	7790	0.0	39.34043	1
CAACGCA	2800	0.0	38.462173	5
TACATGG	8175	0.0	36.973392	2
AACGCAG	2970	0.0	36.658943	6
ACATGGG	8180	0.0	36.441628	3
ATCAACG	3005	0.0	36.0363	3
TATCAAC	3165	0.0	34.402554	2
ATGGGGG	4710	0.0	33.729015	5
CATGGGG	7665	0.0	33.689217	4
TGGGGGG	3245	0.0	28.23523	6
ACGCAGA	4000	0.0	27.068779	7
CGCAGAG	4260	0.0	25.69517	8
GTATAGG	475	0.0	25.055655	1
AGTACAT	5200	0.0	24.600754	2
GAGTACA	5320	0.0	24.384521	1
AGAGTAC	4455	0.0	23.906645	5
ACCGTAT	175	1.7535656E-5	23.79596	8
>>END_MODULE