FastQCFastQC Report
Mon 15 Aug 2016
SRR2921289_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921289_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550362
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17630.11371537744088155No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17450.061.3049131
GTATCAA28950.049.1321871
GTACATG74950.039.7095871
ACATGGG76800.038.020873
TACATGG77900.037.9429782
TCAACGC37300.036.4261444
TATCAAC40950.035.0711752
CAACGCA39050.034.335925
CATGGGG70800.034.256924
ATCAACG40550.033.653623
AACGCAG41250.032.0588466
ATGGGGG42450.031.8665715
TATACCG2255.456968E-1231.7823415
TGGGGGG32450.026.434236
GTGGTAT9500.026.4218581
ACGCAGA52850.025.6695671
AGAGTAC58300.024.3273945
CGCAGAG57250.024.2531222
TGTACGC1751.7304683E-523.8367582
CAGAGTA60800.023.621134
Produced by FastQC (version 0.11.5)