##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921289_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1550362
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.442649523143626	33.0	30.0	34.0	22.0	34.0
2	30.184242776848247	33.0	31.0	34.0	26.0	34.0
3	30.41220244046229	33.0	31.0	34.0	27.0	34.0
4	32.65702139242319	37.0	35.0	37.0	19.0	37.0
5	32.29082433650979	37.0	35.0	37.0	19.0	37.0
6	32.42492463050565	37.0	35.0	37.0	17.0	37.0
7	32.63805549929629	37.0	35.0	37.0	17.0	37.0
8	32.87631082289169	37.0	35.0	37.0	17.0	37.0
9	34.64439143890266	39.0	37.0	39.0	22.0	39.0
10-11	34.814141148970364	39.0	37.0	39.0	26.0	39.0
12-13	34.83745022130316	39.0	37.0	39.0	26.5	39.0
14-15	36.27641028353378	41.0	38.0	41.0	27.0	41.0
16-17	36.292067272030664	41.0	38.0	41.0	27.0	41.0
18-19	36.31369415659053	41.0	38.0	41.0	27.0	41.0
20-21	36.30374970490763	41.0	38.0	41.0	27.0	41.0
22-23	36.26483653495119	41.0	38.0	41.0	27.0	41.0
24-25	36.217968126153764	41.0	38.0	41.0	27.0	41.0
26-27	36.12411810918998	41.0	38.0	41.0	26.5	41.0
28-29	36.0457309325177	40.5	38.0	41.0	25.5	41.0
30-31	35.95710550181184	40.0	38.0	41.0	25.0	41.0
32-33	35.83608699129623	40.0	37.0	41.0	25.0	41.0
34-35	35.758023932475126	40.0	37.0	41.0	24.0	41.0
36-37	35.68121767690385	40.0	37.0	41.0	24.0	41.0
38-39	35.589198200162286	40.0	37.0	41.0	23.5	41.0
40-41	35.461884063205886	40.0	36.0	41.0	22.5	41.0
42-43	35.324751251643164	40.0	36.0	41.0	21.5	41.0
44-45	35.17710895906892	40.0	36.0	41.0	18.5	41.0
46-47	34.973352030042015	40.0	35.0	41.0	15.5	41.0
48-49	34.7695554328602	40.0	35.0	41.0	11.5	41.0
50-51	34.582846457795014	40.0	35.0	41.0	9.0	41.0
52-53	34.392073270629695	39.5	35.0	41.0	8.0	41.0
54-55	34.1852518960088	39.0	35.0	41.0	7.0	41.0
56-57	33.9325799393948	39.0	35.0	41.0	3.5	41.0
58-59	33.858091207085835	39.0	35.0	41.0	2.0	41.0
60-61	33.607630991987676	38.0	35.0	41.0	2.0	41.0
62-63	33.31085836727164	37.0	34.5	40.0	2.0	41.0
64-65	33.000304767531716	37.0	34.0	40.0	2.0	41.0
66-67	32.67348335421018	36.0	34.0	39.0	2.0	41.0
68-69	32.33249944206579	35.5	34.0	39.0	2.0	41.0
70-71	31.99945303096954	35.0	34.0	38.5	2.0	40.5
72-73	31.656836596872214	35.0	33.5	37.0	2.0	39.5
74-75	31.32661275237654	35.0	33.0	37.0	2.0	39.0
76-77	31.037417712766437	35.0	33.0	36.0	2.0	39.0
78-79	30.787972421924685	35.0	33.0	36.0	2.0	37.0
80-81	30.568583014805576	35.0	33.0	35.5	2.0	37.0
82-83	30.377622129541358	35.0	33.0	35.0	2.0	36.5
84-85	30.2127941732318	35.0	33.0	35.0	2.0	36.0
86-87	30.077883745860646	35.0	33.0	35.0	2.0	36.0
88-89	29.965718651514933	35.0	32.5	35.0	2.0	36.0
90-91	29.873906545697068	35.0	32.0	35.0	2.0	35.0
92-93	29.810809668967636	35.0	32.0	35.0	2.0	35.0
94-95	29.742125064984823	35.0	32.0	35.0	2.0	35.0
96-97	29.679726412283067	35.0	32.0	35.0	2.0	35.0
98-99	29.625868990597034	35.0	32.0	35.0	2.0	35.0
100-101	29.577101347943255	35.0	32.0	35.0	2.0	35.0
102-103	28.89691568807801	34.0	30.5	35.0	2.0	35.0
104-105	29.443393542927396	35.0	32.0	35.0	2.0	35.0
106-107	29.509694832561685	35.0	32.0	35.0	2.0	35.0
108-109	29.578302357771925	35.0	32.0	35.0	2.0	35.0
110-111	29.686732840459197	35.0	33.0	35.0	2.0	35.0
112-113	29.650916689134537	35.0	33.0	35.0	2.0	35.0
114-115	29.593235321815165	35.0	33.0	35.0	2.0	35.0
116-117	29.53069057420138	35.0	33.0	35.0	2.0	35.0
118-119	29.454991479409326	35.0	33.0	35.0	2.0	35.0
120-121	29.36398241184962	35.0	32.0	35.0	2.0	35.0
122-123	29.257925245845808	35.0	32.0	35.0	2.0	35.0
124-125	28.737488728438905	34.5	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	96959.0
3	6381.0
4	4686.0
5	4254.0
6	4543.0
7	4724.0
8	4942.0
9	5045.0
10	5135.0
11	5259.0
12	5653.0
13	6648.0
14	7680.0
15	6972.0
16	4828.0
17	4061.0
18	3733.0
19	3312.0
20	3278.0
21	3413.0
22	3718.0
23	4709.0
24	6331.0
25	8010.0
26	9810.0
27	11129.0
28	13604.0
29	16095.0
30	19484.0
31	24128.0
32	31255.0
33	42155.0
34	64400.0
35	111416.0
36	257274.0
37	551707.0
38	183379.0
39	252.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.46279782805828	17.05183960667376	11.070456065041745	27.414906500226216
2	16.33670071892887	18.91525979093915	37.10081903452226	27.647220455609723
3	19.932764089935127	21.88972639938285	27.973789347262123	30.203720163419902
4	13.680501437322915	14.698110578387	31.074495646743706	40.54689233754639
5	14.29126216101651	37.23216039618291	32.13170600581041	16.344871436990168
6	35.571688542997634	32.68736851615235	15.888475884410372	15.852467056439643
7	31.143859350312614	31.26889104371787	17.904435270292325	19.682814335677193
8	28.802286090263152	34.39765972475318	17.39021896538934	19.409835219594324
9	27.991477663052912	15.502564377121795	17.518911112128848	38.98704684769645
10-11	27.46383028129495	24.697741771106795	24.974070036701864	22.86435791089639
12-13	26.804887855419228	21.7148346566865	26.031624946030036	25.448652541864234
14-15	24.1781919319488	23.41778887769437	23.251247128090085	29.152772062266745
16-17	24.64846919622643	26.59495008262586	23.957017780363554	24.799562940784153
18-19	24.6765271594634	25.598892387713324	25.700739569210292	24.023840883612987
20-21	25.15544756643932	24.6554998123019	25.675229398037363	24.513823223221415
22-23	25.015157750254456	24.14677991333637	25.621080754043252	25.21698158236593
24-25	24.690104633627502	24.624087793689473	25.50610760583657	25.179699966846453
26-27	24.37472667673743	24.831103961526406	25.66994031071453	25.124229051021636
28-29	24.463996150576445	25.280547381837277	25.44908866445385	24.806367803132428
30-31	25.08007807208897	24.803626507873645	25.489305078426845	24.62699034161054
32-33	24.65975688258613	24.87167513135642	25.52758646045246	24.94098152560499
34-35	24.798240668953444	25.089172722241642	25.71367203272526	24.39891457607965
36-37	24.769892450924363	25.115779411518087	25.679034960867202	24.435293176690347
38-39	24.708971195114433	25.203758864058845	25.703545365533987	24.383724575292735
40-41	24.675462891892344	25.1358392427059	25.596731602038748	24.59196626336301
42-43	24.583032865872614	25.09136575844867	25.415806114958954	24.909795260719754
44-45	24.28207089699051	25.24010521413709	25.725475727604262	24.75234816126814
46-47	24.317094975238042	25.165832237890246	25.356948893226228	25.160123893645487
48-49	24.412169544919188	25.362689488003447	25.234461370957234	24.99067959612013
50-51	24.402784639974406	25.46160187104689	25.350821292059532	24.78479219691917
52-53	24.561489510191812	25.4390909993924	25.27138823061969	24.728031259796097
54-55	24.21128097824895	25.00467632720616	25.642559608659138	25.141483085885746
56-57	24.303162745216923	25.27903160681183	25.26277733845386	25.15502830951739
58-59	24.35727913867858	25.398971337016775	25.403679914755394	24.840069609549253
60-61	24.781018884621787	25.19208417130967	25.48398374057156	24.54291320349699
62-63	24.50979190666438	25.278676850954806	25.483048475130328	24.728482767250487
64-65	24.35395733383558	24.948850655524325	25.55106484808064	25.146127162559456
66-67	24.268267669099217	25.25152190262661	25.37742798133597	25.1027824469382
68-69	24.097468849210703	25.221915913831737	25.55019408370432	25.13042115325324
70-71	24.121301992695898	25.045924758217758	25.642978865581075	25.18979438350527
72-73	24.168807027004018	25.377782737192987	25.220400138806294	25.2330100969967
74-75	24.058381203873676	25.46595569292849	25.30144572686895	25.174217376328883
76-77	24.067959612013194	25.00067726118158	25.39019919218866	25.541163934616563
78-79	23.940666760408213	24.97997241934464	25.62814362065118	25.451217199595966
80-81	24.15581006242413	25.30544479289353	25.23517088267127	25.303574262011065
82-83	24.256979982739516	25.306444559399676	25.25806876071524	25.17850669714557
84-85	24.226567730633235	25.374106176492973	25.081948602971437	25.31737748990236
86-87	23.853267817451666	25.613211624123917	25.223786444714204	25.30973411371022
88-89	23.760837791431936	25.21940037230015	25.334309019441907	25.685452816826004
90-91	24.022581851956964	25.02460718800795	25.45644237467427	25.49636858536082
92-93	24.00112619113579	25.277024636559865	25.39802890750752	25.32382026479682
94-95	24.026347420487042	25.121318557845097	25.260511990598317	25.59182203106955
96-97	23.75121625095099	25.057817272585662	25.299374306007287	25.891592170456057
98-99	23.611339552530023	25.112344802275082	25.32681098144239	25.949504663752503
100-101	23.6604418838955	25.170798819888518	25.394166007680784	25.77459328853519
102-103	23.455973508122618	25.07249919696174	25.49507792373652	25.97644937117912
104-105	23.609461406259122	25.12984874817905	25.352732249865596	25.907957595696228
106-107	23.614676591243324	24.969942464975876	25.397133568977527	26.018247374803273
108-109	23.58517309717275	24.944504735198173	25.459031223443624	26.011290944185454
110-111	23.365434510792184	25.32469103113893	25.428957237954965	25.88091722011393
112-113	23.39382391670534	25.40704478952031	25.382630971105584	25.816500322668762
114-115	23.356074302790514	25.312975897840435	25.333132519629327	25.997817279739728
116-117	23.114222226522312	25.20809750024994	25.459362533097256	26.218317740130487
118-119	23.229321973387464	25.00726444414989	25.506729245700754	26.256684336761893
120-121	23.052864156433294	25.075297003503376	25.63207073451626	26.23976810554706
122-123	23.06800440034354	25.21967047735213	25.368928685344155	26.343396436960177
124-125	22.859690652493615	25.149158905028507	25.254811785649782	26.73633865682809
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	35.0
1	40.0
2	56.5
3	61.0
4	48.5
5	73.0
6	180.0
7	387.0
8	538.5
9	644.0
10	664.0
11	561.5
12	526.5
13	562.0
14	673.0
15	720.5
16	751.0
17	813.5
18	863.0
19	914.0
20	931.0
21	1007.5
22	1109.0
23	1228.0
24	1372.0
25	1548.0
26	1888.0
27	2368.0
28	2828.0
29	3586.5
30	4769.5
31	6056.5
32	7815.5
33	9911.0
34	12076.0
35	14383.5
36	17304.0
37	20759.5
38	23860.0
39	27284.5
40	30933.5
41	33795.0
42	36949.5
43	41657.5
44	46899.0
45	51966.5
46	55624.5
47	59470.5
48	65225.5
49	69618.5
50	72214.0
51	74902.5
52	75706.0
53	74871.5
54	74751.5
55	73025.5
56	67975.5
57	61868.5
58	56648.0
59	50912.5
60	43646.0
61	36495.5
62	30272.0
63	23617.5
64	17287.0
65	12896.0
66	9755.0
67	7324.0
68	5439.0
69	4042.0
70	3115.0
71	2354.0
72	1767.5
73	1340.5
74	1018.0
75	766.5
76	555.0
77	382.0
78	279.0
79	211.5
80	175.5
81	146.5
82	134.5
83	123.5
84	110.5
85	103.0
86	97.5
87	87.0
88	76.0
89	69.5
90	65.0
91	63.0
92	61.5
93	60.0
94	56.5
95	48.5
96	43.0
97	40.0
98	25.5
99	12.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3473382345542525
2	0.0
3	0.0
4	0.016641274747446082
5	0.1802159753657533
6	0.04785979016513563
7	0.022897878043966507
8	0.007546624594772061
9	0.005353588387744281
10-11	0.0020640340772026147
12-13	0.05734144670728514
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	1.2900212982516342E-4
92-93	2.2575372719403598E-4
94-95	9.675159736887256E-5
96-97	2.2575372719403598E-4
98-99	6.450106491258171E-5
100-101	0.0
102-103	0.0
104-105	3.2250532456290855E-5
106-107	1.2900212982516342E-4
108-109	2.902547921066177E-4
110-111	2.2575372719403598E-4
112-113	6.772611815821079E-4
114-115	1.9350319473774512E-4
116-117	4.5150745438807195E-4
118-119	1.6125266228145428E-4
120-121	9.675159736887256E-5
122-123	0.0018382803500085786
124-125	1.2900212982516342E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1550362.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.12479347367646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.01630681891675	50.952461225988856
2	9.428966632830091	11.71545209469512
3	3.247940960518615	6.0533298416074
4	1.5566822794280049	3.8683426045438694
5	0.9047167769181453	2.8102671459105
6	0.584291846584453	2.177940617844732
7	0.42196904377925654	1.8350317787949737
8	0.3060645646021614	1.5211358292415988
9	0.23134463383133	1.2935013838213212
>10	1.2274836741947233	13.356277040990156
>50	0.057743420290801054	2.3868106726530938
>100	0.015659769925271384	1.525414254691926
>500	5.184863627791206E-4	0.22734423722197503
>1k	3.110918176674724E-4	0.2766912719944411
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1763	0.11371537744088155	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.450106491258171E-5
2	0.0	0.0	0.0	0.0	6.450106491258171E-5
3	0.0	0.0	0.0	0.0	6.450106491258171E-5
4	0.0	0.0	0.0	0.0	6.450106491258171E-5
5	6.450106491258171E-5	0.0	0.0	0.0	6.450106491258171E-5
6	6.450106491258171E-5	0.0	0.0	0.0	6.450106491258171E-5
7	6.450106491258171E-5	0.0	0.0	0.0	6.450106491258171E-5
8	6.450106491258171E-5	0.0	0.0	0.0	6.450106491258171E-5
9	6.450106491258171E-5	0.0	0.0	0.0	6.450106491258171E-5
10-11	6.450106491258171E-5	0.0	0.0	0.0	6.450106491258171E-5
12-13	6.450106491258171E-5	0.0	0.0	0.0	6.450106491258171E-5
14-15	6.450106491258171E-5	0.0	0.0	0.0	1.2900212982516342E-4
16-17	6.450106491258171E-5	0.0	0.0	0.0	1.2900212982516342E-4
18-19	6.450106491258171E-5	0.0	0.0	0.0	1.2900212982516342E-4
20-21	6.450106491258171E-5	0.0	0.0	6.450106491258171E-5	2.902547921066177E-4
22-23	6.450106491258171E-5	0.0	0.0	9.675159736887256E-5	5.805095842132354E-4
24-25	6.450106491258171E-5	0.0	0.0	1.6125266228145425E-4	7.417622464946897E-4
26-27	6.450106491258171E-5	0.0	0.0	5.160085193006537E-4	9.030149087761439E-4
28-29	6.450106491258171E-5	0.0	0.0	0.0033540553754542487	9.030149087761439E-4
30-31	6.450106491258171E-5	0.0	0.0	0.012255202333390523	9.352654412324347E-4
32-33	6.450106491258171E-5	0.0	0.0	0.030025245716806787	0.0010320170386013074
34-35	9.675159736887256E-5	0.0	0.0	0.050149577969532275	0.0010320170386013074
36-37	1.2900212982516342E-4	0.0	0.0	0.08023932475125164	0.001096518103513889
38-39	1.2900212982516342E-4	0.0	0.0	0.13271094105763687	0.0011287686359701798
40-41	1.2900212982516342E-4	0.0	0.0	0.19876003152812052	0.0011610191684264708
42-43	1.2900212982516342E-4	0.0	0.0	0.25442445054767854	0.0011932697008827616
44-45	1.2900212982516342E-4	0.0	0.0	0.31602296753919407	0.0012255202333390524
46-47	1.6125266228145425E-4	0.0	0.0	0.38091103884125127	0.0012255202333390524
48-49	3.225053245629085E-4	0.0	0.0	0.4546680065687885	0.0012255202333390524
50-51	3.225053245629085E-4	0.0	0.0	0.5319402823340613	0.0012255202333390524
52-53	3.225053245629085E-4	0.0	0.0	0.6165656794993686	0.0013545223631642156
54-55	3.225053245629085E-4	0.0	0.0	0.7072541767664584	0.0014190234280767975
56-57	3.225053245629085E-4	0.0	0.0	0.7995874511888191	0.0014190234280767975
58-59	3.225053245629085E-4	0.0	0.0	0.9057562040349285	0.0014835244929893793
60-61	3.225053245629085E-4	0.0	0.0	1.0203100953196738	0.0014835244929893793
62-63	3.225053245629085E-4	0.0	0.0	1.1434748787702484	0.0016125266228145428
64-65	3.225053245629085E-4	0.0	0.0	1.2579320184576246	0.0016125266228145428
66-67	3.225053245629085E-4	0.0	0.0	1.3914492228266688	0.0016125266228145428
68-69	3.225053245629085E-4	0.0	0.0	1.5396726699957815	0.0016125266228145428
70-71	3.225053245629085E-4	0.0	0.0	1.698700045537752	0.0016770276877271244
72-73	3.225053245629085E-4	0.0	0.0	1.8497937900954744	0.0018705308824648694
74-75	3.225053245629085E-4	0.0	0.0	2.00233880861373	0.0018705308824648694
76-77	3.225053245629085E-4	0.0	0.0	2.187392363847927	0.0019027814149211605
78-79	3.5475585701919937E-4	0.0	0.0	2.3906674699199284	0.0019350319473774513
80-81	3.8700638947549023E-4	0.0	0.0	2.5892662487857674	0.001967282479833742
82-83	3.8700638947549023E-4	0.0	0.0	2.803893542282383	0.0022575372719403596
84-85	3.8700638947549023E-4	0.0	0.0	3.0245839358807816	0.0022575372719403596
86-87	3.8700638947549023E-4	0.0	0.0	3.2685592139126216	0.0022897878043966506
88-89	3.8700638947549023E-4	0.0	0.0	3.5515253856841174	0.0023220383368529416
90-91	3.8700638947549023E-4	0.0	0.0	3.8474240209705863	0.002386539401765523
92-93	3.8700638947549023E-4	0.0	0.0	4.1264556277824145	0.002451040466678105
94-95	3.8700638947549023E-4	0.0	0.0	4.414936640603936	0.0025155415315906864
96-97	3.8700638947549023E-4	0.0	0.0	4.73818372741334	0.002547792064046977
98-99	3.8700638947549023E-4	0.0	0.0	5.112354404971226	0.002580042596503268
100-101	4.192569219317811E-4	0.0	0.0	5.509164956313429	0.002580042596503268
102-103	4.5150745438807195E-4	0.0	0.0	5.912586866809171	0.00264454366141585
104-105	4.5150745438807195E-4	0.0	0.0	6.335907355830445	0.002676794193872141
106-107	4.5150745438807195E-4	0.0	0.0	6.790059353879933	0.0027412952587847223
108-109	4.5150745438807195E-4	0.0	0.0	7.257047063847024	0.0028057963236973044
110-111	4.5150745438807195E-4	0.0	0.0	7.762961166488859	0.0029025479210661766
112-113	4.5150745438807195E-4	0.0	0.0	8.268230258481568	0.0029670489859787586
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1745	0.0	61.304913	1
GTATCAA	2895	0.0	49.132187	1
GTACATG	7495	0.0	39.709587	1
ACATGGG	7680	0.0	38.02087	3
TACATGG	7790	0.0	37.942978	2
TCAACGC	3730	0.0	36.426144	4
TATCAAC	4095	0.0	35.071175	2
CAACGCA	3905	0.0	34.33592	5
CATGGGG	7080	0.0	34.25692	4
ATCAACG	4055	0.0	33.65362	3
AACGCAG	4125	0.0	32.058846	6
ATGGGGG	4245	0.0	31.866571	5
TATACCG	225	5.456968E-12	31.782341	5
TGGGGGG	3245	0.0	26.43423	6
GTGGTAT	950	0.0	26.421858	1
ACGCAGA	5285	0.0	25.669567	1
AGAGTAC	5830	0.0	24.327394	5
CGCAGAG	5725	0.0	24.253122	2
TGTACGC	175	1.7304683E-5	23.836758	2
CAGAGTA	6080	0.0	23.62113	4
>>END_MODULE