FastQCFastQC Report
Mon 15 Aug 2016
SRR2921288_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921288_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365451
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12300.060.4738461
GTATCAA22700.054.0012781
TCAACGC26000.045.3163074
CAACGCA26850.044.1033365
AACGCAG28150.042.274896
ATCAACG28700.040.8457643
TATCAAC29700.040.0715562
GTACATG66650.036.5162241
TACATGG68550.035.1568762
ACATGGG68900.034.8919143
ACGCAGA35550.033.133027
CATGGGG63500.031.580494
ATGGGGG38650.031.1002985
CGCAGAG38500.030.5942578
GTATAGG4200.029.7531321
TACACCG2103.1359377E-928.3363175
AGTACAT47250.026.5731662
GAGTACA48300.026.4882951
GTATATA3850.026.2754941
GCAGAGT45350.025.4483649
Produced by FastQC (version 0.11.5)