##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921288_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1365451
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.633867491400277	33.0	31.0	34.0	27.0	34.0
2	30.640521703085646	33.0	31.0	34.0	27.0	34.0
3	30.792585746394415	33.0	31.0	34.0	27.0	34.0
4	34.22741497131717	37.0	35.0	37.0	31.0	37.0
5	34.23744901867588	37.0	35.0	37.0	31.0	37.0
6	34.30175231480295	37.0	35.0	37.0	31.0	37.0
7	34.19306954259069	37.0	35.0	37.0	30.0	37.0
8	34.18239028716519	37.0	35.0	37.0	30.0	37.0
9	35.78606189456817	39.0	37.0	39.0	30.0	39.0
10-11	35.830527788986934	39.0	37.0	39.0	30.5	39.0
12-13	35.786099244864886	39.0	37.0	39.0	30.0	39.0
14-15	37.12742602993443	41.0	38.0	41.0	31.0	41.0
16-17	37.120280771701076	41.0	38.0	41.0	31.0	41.0
18-19	37.11018740328287	41.0	38.0	41.0	31.0	41.0
20-21	37.147680509956054	41.0	38.0	41.0	31.0	41.0
22-23	37.09594851810867	41.0	38.0	41.0	30.0	41.0
24-25	37.087882684915094	41.0	38.0	41.0	30.0	41.0
26-27	37.06426265021594	41.0	38.0	41.0	30.0	41.0
28-29	37.00111721328704	41.0	38.0	41.0	30.0	41.0
30-31	36.930030810332994	40.0	38.0	41.0	30.0	41.0
32-33	36.86415111197692	40.0	38.0	41.0	30.0	41.0
34-35	36.75751967664896	40.0	38.0	41.0	30.0	41.0
36-37	36.647854078981965	40.0	38.0	41.0	30.0	41.0
38-39	36.54495547624924	40.0	37.0	41.0	29.5	41.0
40-41	36.45065696242487	40.0	37.0	41.0	29.0	41.0
42-43	36.253770732161016	40.0	37.0	41.0	28.5	41.0
44-45	36.001315682510764	40.0	36.0	41.0	27.5	41.0
46-47	35.786931570594625	40.0	36.0	41.0	27.0	41.0
48-49	35.58412971245398	40.0	35.0	41.0	27.0	41.0
50-51	35.403946388409395	39.5	35.0	41.0	26.0	41.0
52-53	35.208772412924375	39.0	35.0	41.0	26.0	41.0
54-55	35.002841185806005	39.0	35.0	41.0	26.0	41.0
56-57	34.7553852170455	38.5	35.0	40.5	25.5	41.0
58-59	34.48918525820406	38.0	34.5	40.0	25.0	41.0
60-61	34.19667457858246	38.0	34.0	40.0	24.0	41.0
62-63	33.868226322292045	37.0	34.0	40.0	23.0	41.0
64-65	33.51453256103661	36.5	34.0	39.5	22.5	41.0
66-67	33.16141992645653	36.0	33.0	39.0	22.0	41.0
68-69	32.79692094406903	35.5	33.0	39.0	21.5	40.0
70-71	32.74019756109886	35.0	33.0	38.5	22.0	40.0
72-73	32.44369552624005	35.0	33.0	37.0	21.0	39.5
74-75	32.11721804737043	35.0	33.0	37.0	20.0	39.0
76-77	31.821338151277487	35.0	33.0	36.5	20.0	39.0
78-79	31.537994772423176	35.0	33.0	36.0	19.0	38.0
80-81	31.265176487475564	35.0	33.0	35.5	18.0	37.0
82-83	31.049648797357065	35.0	33.0	35.0	17.5	37.0
84-85	30.842038271604032	35.0	32.0	35.0	16.0	36.0
86-87	30.674343129119976	35.0	32.0	35.0	12.5	36.0
88-89	30.535650858214613	35.0	32.0	35.0	10.5	36.0
90-91	30.415847584424487	35.0	32.0	35.0	7.5	35.5
92-93	30.306167705761688	35.0	32.0	35.0	7.0	35.0
94-95	30.18412597742431	35.0	32.0	35.0	6.5	35.0
96-97	30.095452345049367	35.0	32.0	35.0	2.0	35.0
98-99	30.008412238886642	35.0	32.0	35.0	2.0	35.0
100-101	29.91636170027339	35.0	31.0	35.0	2.0	35.0
102-103	29.077337084963137	33.5	29.5	34.5	2.0	35.0
104-105	29.67400514555264	34.0	31.0	35.0	2.0	35.0
106-107	29.74765224090795	35.0	31.0	35.0	2.0	35.0
108-109	29.68280809783727	35.0	31.0	35.0	2.0	35.0
110-111	29.600657218750435	35.0	31.0	35.0	2.0	35.0
112-113	29.514750071588068	34.5	31.0	35.0	2.0	35.0
114-115	29.385262451746712	34.0	31.0	35.0	2.0	35.0
116-117	29.246650374125473	34.0	31.0	35.0	2.0	35.0
118-119	29.24093834198371	34.5	31.0	35.0	2.0	35.0
120-121	29.406056680173805	35.0	31.5	35.0	2.0	35.0
122-123	29.270716781488314	35.0	32.0	35.0	2.0	35.0
124-125	28.698487166511285	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	45889.0
3	6540.0
4	4068.0
5	3387.0
6	3574.0
7	4173.0
8	4386.0
9	4624.0
10	5045.0
11	5530.0
12	5824.0
13	5890.0
14	5934.0
15	5113.0
16	4015.0
17	3695.0
18	3521.0
19	3469.0
20	3367.0
21	3656.0
22	4103.0
23	5457.0
24	7888.0
25	10054.0
26	10821.0
27	12093.0
28	14113.0
29	17060.0
30	20780.0
31	26055.0
32	34254.0
33	45976.0
34	72431.0
35	119963.0
36	260984.0
37	447520.0
38	124109.0
39	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.06602898079398	17.102960705336535	11.400630947175204	25.430379366694282
2	16.839607267240652	18.956716631082433	38.14620176444091	26.057474337236002
3	20.6893394672407	22.645016835041496	28.15696396488116	28.508679732836644
4	13.732431246653615	14.027462518650033	34.25856546537374	37.981540769322606
5	14.406522691283987	37.95972536961869	32.78557648502995	14.84817545406738
6	35.47152367947584	33.33279611798421	16.290371733486488	14.905308469053466
7	31.181937237966608	31.51329686717196	18.602283480946088	18.702482413915348
8	28.187019881519504	35.24992529048816	18.2260446147626	18.33701021322974
9	27.890012004256786	15.84151744352543	17.664128365714554	38.60434218650323
10-11	27.204992690551034	25.35683001356423	25.78679113725651	21.651386158628224
12-13	26.529536811382975	22.497596809661832	26.43641204140262	24.536454337552573
14-15	23.963876670050347	24.027082610090392	23.40344578570916	28.6055949341501
16-17	24.58807843418721	27.27769418400988	24.16366052572892	23.97056685607399
18-19	24.534689635624982	26.59195725142982	25.590586935935004	23.282766177010192
20-21	25.140124902134115	25.383535388083544	25.641044420308056	23.83529528947428
22-23	25.0941398730105	24.973295146399725	25.473628934182468	24.458936046407302
24-25	24.540889397821637	25.313419040570622	25.547638778673697	24.598052782934037
26-27	24.32920657566512	25.594588537554618	25.618427740482996	24.457777146297268
28-29	24.45069421938541	25.94551343671096	25.484800046873314	24.11899229703032
30-31	24.97790921777941	25.490071140924357	25.448837455420197	24.083182185876034
32-33	24.531205254573326	25.453524081141794	25.535407067571043	24.47986359671384
34-35	24.557250630877338	25.71687111206161	25.662306768066017	24.06357148899503
36-37	24.58494766986238	25.727421870994675	25.609138914726408	24.078491544416533
38-39	24.59834221064215	25.87740708121776	25.569395897456555	23.954854810683532
40-41	24.56373563073173	25.59495755913871	25.646701756043505	24.19460505408605
42-43	24.337183944981845	25.54107227541303	25.573444512191774	24.548299267413352
44-45	24.17817913492155	25.665068195925272	25.786097882072045	24.370654787081133
46-47	24.2833909442837	25.59317794551014	25.577650637773182	24.545780472432988
48-49	24.172606808107034	25.833051050732138	25.55817798635156	24.436164154809273
50-51	24.17181814220233	25.86694380451528	25.664143935906747	24.297094117375643
52-53	24.36306776256644	25.80942842442741	25.530021587199286	24.297482225806867
54-55	24.123174500871553	25.45368322371794	25.790622390836248	24.632519884574258
56-57	24.213983649805254	25.755658189818703	25.481885443170547	24.548472717205495
58-59	24.21075567290245	25.90293125136181	25.636776777033372	24.249536298702367
60-61	24.525822118889884	25.794031666421922	25.679485597333212	24.000660617354974
62-63	24.457974186073255	25.75178319406879	25.648881965332905	24.141360654525055
64-65	24.273506026209702	25.556636630522465	25.697326921098085	24.472530422169744
66-67	24.25774987567828	25.82273882770657	25.49364329662394	24.425867999991212
68-69	24.10512974597905	25.771992328872667	25.685020172047405	24.43785775310088
70-71	24.054687471391063	25.69443274656784	25.813995217501162	24.43688456453993
72-73	24.156101726631192	25.961720501140874	25.535656283540888	24.346521488687053
74-75	23.990755842780484	26.00939063412048	25.66169109046926	24.33816243262978
76-77	24.038265765732774	25.71697043645882	25.61630381764145	24.628459980166955
78-79	24.057008308829182	25.664358455121704	25.77205465005145	24.506578585997662
80-81	24.208601704023426	25.970153407515877	25.4274980765928	24.39374681186789
82-83	24.286934763351585	25.921851660751997	25.572647531969793	24.21856604392663
84-85	24.23498021189586	26.0025246449512	25.33034799744093	24.432147145712005
86-87	23.982558618575798	26.173680681513154	25.50393720106623	24.33982349884482
88-89	23.971578899570382	25.850340634278457	25.590928332774276	24.587152133376886
90-91	24.210081485637115	25.725065438986842	25.611288756212492	24.453564319163554
92-93	24.256512042655572	25.84364505934354	25.650581716842876	24.249261181158012
94-95	24.229449640628943	25.73021335219023	25.576372437831026	24.46396456934981
96-97	24.003194815638924	25.721415298933525	25.620634319585566	24.654755565841988
98-99	23.93002955595032	25.88491159764358	25.49794873428684	24.68711011211926
100-101	23.982416288224048	25.89356830039378	25.624920121477608	24.499095289904563
102-103	23.82404262526206	25.841821460940572	25.76675119700451	24.56738471679285
104-105	23.921044035640996	25.868577128652976	25.717668306730513	24.49271052897552
106-107	23.934194861518666	25.828670659007173	25.67929662738412	24.557837852090046
108-109	23.90607851545492	25.828335009918526	25.650361860427306	24.615224614199256
110-111	23.784672568640197	26.199269424786458	25.584853839181903	24.431204167391442
112-113	23.750393660419366	26.247995078329268	25.655380513992338	24.346230747259025
114-115	23.81556937625236	26.265394485522446	25.5921970025428	24.326839135682395
116-117	23.654010586505592	26.183456708296184	25.63501639608398	24.527516309114244
118-119	23.743318645814423	26.037835488786325	25.68676528024556	24.532080585153686
120-121	23.606945925071695	26.183780561092522	25.74630022225583	24.46297329157995
122-123	23.641753223807196	26.351344176091366	25.57114896250442	24.435753637597017
124-125	23.613901231331013	26.23310700708127	25.585290526887803	24.56770123469991
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	267.0
1	230.0
2	177.0
3	149.0
4	144.5
5	135.5
6	127.5
7	151.0
8	183.0
9	213.5
10	231.0
11	245.0
12	253.5
13	266.0
14	287.5
15	349.5
16	409.0
17	441.5
18	484.0
19	529.0
20	618.0
21	708.5
22	817.0
23	1011.0
24	1227.0
25	1574.0
26	2106.0
27	2868.0
28	3751.0
29	4826.5
30	6145.0
31	7530.0
32	9581.0
33	11877.0
34	14288.0
35	16847.0
36	19859.0
37	22845.0
38	25166.0
39	28093.5
40	31040.0
41	33417.0
42	35887.0
43	39363.5
44	43476.5
45	47472.0
46	50856.0
47	54030.0
48	58170.0
49	61474.5
50	62189.0
51	62215.5
52	61971.0
53	61101.0
54	60686.5
55	58878.0
56	55145.0
57	50813.0
58	46421.0
59	41525.5
60	35354.5
61	29495.5
62	24394.5
63	18906.5
64	13860.0
65	10377.5
66	7829.5
67	5691.0
68	4146.0
69	3128.5
70	2443.0
71	1835.0
72	1341.5
73	1060.0
74	798.0
75	546.0
76	382.0
77	264.0
78	172.0
79	114.0
80	74.0
81	49.5
82	40.5
83	30.5
84	22.0
85	16.5
86	14.5
87	13.0
88	9.0
89	5.5
90	3.5
91	3.5
92	3.5
93	2.5
94	1.5
95	2.0
96	1.5
97	1.5
98	2.5
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014793646934236382
2	0.010692437883160948
3	0.010545966131336825
4	0.012596570656874542
5	0.00915448448900766
6	0.02834228397796772
7	0.012230391277314234
8	0.01193744777366599
9	0.007470059343030251
10-11	0.006957408211645822
12-13	0.005749016259096812
14-15	0.005346218941580474
16-17	0.003771647609471156
18-19	0.005272983065668413
20-21	0.0045406243065478
22-23	0.00457724224450383
24-25	0.004870185748152076
26-27	0.0038815014233392485
28-29	0.005016657499976198
30-31	0.00457724224450383
32-33	0.006444757080261393
34-35	0.006627846770041547
36-37	0.005932105948876965
38-39	0.005749016259096812
40-41	0.005895488010920934
42-43	0.006115195638657117
44-45	0.006115195638657117
46-47	0.008385507791931018
48-49	0.0068475543977777305
50-51	0.004796949872240015
52-53	0.004284298740855585
54-55	0.0051997471897563515
56-57	0.00622504945252521
58-59	0.005749016259096812
60-61	0.00450400636859177
62-63	0.004687096058371923
64-65	0.0029660529744384824
66-67	0.003075906788306574
68-69	0.005236365127712382
70-71	0.004101209051075432
72-73	0.00340546822991085
74-75	0.0035153220437789423
76-77	0.0038815014233392485
78-79	0.0026364915328342064
80-81	0.0025998735948781757
82-83	0.0044307704926797075
84-85	0.008092564288282773
86-87	0.00567578038318475
88-89	0.004174444926987494
90-91	0.004174444926987494
92-93	0.0070306440875578845
94-95	0.005822252135008872
96-97	0.008348889853974988
98-99	0.005456072755448566
100-101	0.007067262025513915
102-103	0.004650478120415892
104-105	0.004174444926987494
106-107	0.006334903266393302
108-109	0.005602544507272689
110-111	0.006481375018217423
112-113	0.004467388430635739
114-115	0.0019773686496256547
116-117	0.0024534018430540532
118-119	0.15002369180585756
120-121	0.00970375355834812
122-123	0.0017210430839334403
124-125	0.0013182457664171032
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1365451.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.07289751411781
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.1565412146619	53.4616418657609
2	9.941657614163086	12.938649340937669
3	3.093050653849706	6.03821304611811
4	1.4966459772739158	3.8956436117624884
5	0.8582637367170955	2.792485408973768
6	0.5345392010166592	2.087040878702129
7	0.3659082855254456	1.6667498654494715
8	0.2705307827456918	1.408337752001958
9	0.19898428583224342	1.1653635634993351
>10	1.0322826832345666	11.563840784835747
>50	0.040236695606377826	1.7448051698137734
>100	0.011246408914806692	1.1898072434273372
>500	1.1246045847104762E-4	0.047421468717332146
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.323587591206129E-5	0.0
5	0.0	0.0	0.0	7.323587591206129E-5	0.0
6	0.0	0.0	0.0	7.323587591206129E-5	7.323587591206129E-5
7	0.0	0.0	0.0	7.323587591206129E-5	7.323587591206129E-5
8	0.0	0.0	0.0	7.323587591206129E-5	7.323587591206129E-5
9	0.0	0.0	0.0	7.323587591206129E-5	7.323587591206129E-5
10-11	0.0	0.0	0.0	1.4647175182412258E-4	7.323587591206129E-5
12-13	0.0	0.0	0.0	1.4647175182412258E-4	1.4647175182412258E-4
14-15	0.0	0.0	0.0	1.4647175182412258E-4	1.4647175182412258E-4
16-17	0.0	0.0	0.0	1.4647175182412258E-4	2.1970762773618386E-4
18-19	0.0	0.0	0.0	1.4647175182412258E-4	2.9294350364824517E-4
20-21	0.0	0.0	0.0	2.9294350364824517E-4	2.9294350364824517E-4
22-23	0.0	0.0	0.0	4.3941525547236773E-4	4.027973175163371E-4
24-25	0.0	0.0	0.0	8.055946350326742E-4	5.12651131384429E-4
26-27	0.0	0.0	0.0	0.001354863704373134	5.12651131384429E-4
28-29	0.0	0.0	0.0	0.0036617937956030643	5.492690693404596E-4
30-31	0.0	0.0	0.0	0.014866882810148443	6.22504945252521E-4
32-33	0.0	0.0	0.0	0.03277305447064743	6.591228832085516E-4
34-35	0.0	0.0	0.0	0.052986156222376346	7.323587591206128E-4
36-37	0.0	0.0	0.0	0.08491699812003506	7.323587591206128E-4
38-39	0.0	0.0	0.0	0.13808624403219155	8.788305109447355E-4
40-41	0.0	0.0	0.0	0.20077615381291603	8.788305109447355E-4
42-43	0.0	0.0	0.0	0.2577536652724997	8.788305109447355E-4
44-45	0.0	0.0	0.0	0.31758737589265373	8.788305109447355E-4
46-47	0.0	0.0	0.0	0.38913882665873767	9.154484489007661E-4
48-49	0.0	0.0	0.0	0.46153248999781027	9.520663868567968E-4
50-51	0.0	0.0	0.0	0.5399314951616718	9.520663868567968E-4
52-53	0.0	0.0	0.0	0.6167925469313802	0.0010253022627688582
54-55	0.0	0.0	0.0	0.7065431128616113	0.0010985381386809194
56-57	0.0	0.0	0.0	0.8015666618575108	0.0010985381386809194
58-59	0.0	0.0	0.0	0.9069530872949669	0.0010985381386809194
60-61	0.0	0.0	0.0	1.0140239378784006	0.0010985381386809194
62-63	0.0	0.0	0.0	1.1317872263449953	0.0011717740145929807
64-65	0.0	0.0	0.0	1.2495871327495458	0.0011717740145929807
66-67	0.0	0.0	0.0	1.382510247529937	0.0011717740145929807
68-69	0.0	0.0	0.0	1.5289453814161036	0.0011717740145929807
70-71	0.0	0.0	0.0	1.6835096975285089	0.0011717740145929807
72-73	0.0	0.0	0.0	1.831812346250433	0.0013914816423291644
74-75	0.0	0.0	0.0	1.982092363621983	0.0013914816423291644
76-77	0.0	0.0	0.0	2.1681114884386186	0.0013914816423291644
78-79	0.0	0.0	0.0	2.3715973696602806	0.0013914816423291644
80-81	0.0	0.0	0.0	2.581235064458556	0.001428099580285195
82-83	0.0	0.0	0.0	2.7914952642020845	0.0016111892700653484
84-85	0.0	0.0	0.0	3.02299386796011	0.0016111892700653484
86-87	0.0	0.0	0.0	3.273131002137755	0.001647807208021379
88-89	0.0	0.0	0.0	3.5567369316072126	0.0016844251459774097
90-91	0.0	0.0	0.0	3.8576997636678287	0.0016844251459774097
92-93	0.0	0.0	0.0	4.156026104195609	0.001757661021889471
94-95	0.0	0.0	0.0	4.450617415051877	0.0018308968978015322
96-97	0.0	0.0	0.0	4.777762072751054	0.0018308968978015322
98-99	0.0	0.0	0.0	5.149177817439073	0.0018308968978015322
100-101	0.0	0.0	0.0	5.550803360940817	0.0018308968978015322
102-103	0.0	0.0	0.0	5.963341049953458	0.0018308968978015322
104-105	0.0	0.0	0.0	6.379687004513527	0.0018675148357575628
106-107	0.0	0.0	0.0	6.822690817905586	0.0019773686496256547
108-109	0.0	0.0	0.0	7.299456370093105	0.002160458339405808
110-111	0.0	0.0	0.0	7.8247773080103205	0.0023435480291859614
112-113	0.0	0.0	0.0	8.349768684485932	0.0024167839050980226
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1230	0.0	60.473846	1
GTATCAA	2270	0.0	54.001278	1
TCAACGC	2600	0.0	45.316307	4
CAACGCA	2685	0.0	44.103336	5
AACGCAG	2815	0.0	42.27489	6
ATCAACG	2870	0.0	40.845764	3
TATCAAC	2970	0.0	40.071556	2
GTACATG	6665	0.0	36.516224	1
TACATGG	6855	0.0	35.156876	2
ACATGGG	6890	0.0	34.891914	3
ACGCAGA	3555	0.0	33.13302	7
CATGGGG	6350	0.0	31.58049	4
ATGGGGG	3865	0.0	31.100298	5
CGCAGAG	3850	0.0	30.594257	8
GTATAGG	420	0.0	29.753132	1
TACACCG	210	3.1359377E-9	28.336317	5
AGTACAT	4725	0.0	26.573166	2
GAGTACA	4830	0.0	26.488295	1
GTATATA	385	0.0	26.275494	1
GCAGAGT	4535	0.0	25.448364	9
>>END_MODULE