FastQCFastQC Report
Mon 15 Aug 2016
SRR2921288_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921288_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365451
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18960.1388552207292682No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14330.10494701018198382No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18850.075.4637761
GTATCAA31000.046.1451262
GTACATG63550.041.2878151
TACATGG66500.039.5269432
TCAACGC37800.038.789985
ACATGGG67350.038.4085923
CAACGCA38900.037.3633426
AACGCAG40000.036.322537
ATCAACG40200.036.0293584
ATGGGGG34750.034.990625
CATGGGG60300.034.596094
TATCAAC42350.034.0594983
ACGCAGA49150.029.3150358
CGCAGAG51950.027.6204059
TATACCG1556.74036E-626.917995
GAGTACT27850.025.3230912-13
ATACCGT1951.4508605E-624.437736
GTACTTT30300.023.56064214-15
GTGGTAT9900.023.5451971
GAGTACA43100.023.2972661
Produced by FastQC (version 0.11.5)