##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921288_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1365451
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.32672574848896	33.0	30.0	34.0	19.0	34.0
2	30.07342043031936	33.0	31.0	34.0	26.0	34.0
3	30.305800794023366	33.0	31.0	34.0	27.0	34.0
4	32.51553076602529	37.0	35.0	37.0	19.0	37.0
5	32.1478910630993	37.0	33.0	37.0	19.0	37.0
6	32.28881959147564	37.0	35.0	37.0	17.0	37.0
7	32.50468819459651	37.0	35.0	37.0	17.0	37.0
8	32.749540627968344	37.0	35.0	37.0	17.0	37.0
9	34.50822695212058	39.0	37.0	39.0	17.0	39.0
10-11	34.680353597456076	39.0	37.0	39.0	24.0	39.0
12-13	34.705743010917274	39.0	37.0	39.0	25.0	39.0
14-15	36.13939313823784	41.0	38.0	41.0	27.0	41.0
16-17	36.15741941673484	41.0	38.0	41.0	27.0	41.0
18-19	36.18602791312175	41.0	38.0	41.0	27.0	41.0
20-21	36.173363233100275	41.0	38.0	41.0	27.0	41.0
22-23	36.131879869728024	41.0	38.0	41.0	26.0	41.0
24-25	36.08604519678846	41.0	38.0	41.0	26.0	41.0
26-27	35.994686737202585	41.0	38.0	41.0	25.0	41.0
28-29	35.91726652952028	40.0	38.0	41.0	25.0	41.0
30-31	35.82498676261543	40.0	37.5	41.0	24.0	41.0
32-33	35.697357503125346	40.0	37.0	41.0	24.0	41.0
34-35	35.616454563364044	40.0	37.0	41.0	23.5	41.0
36-37	35.537704758354565	40.0	37.0	41.0	23.0	41.0
38-39	35.43993083603878	40.0	36.5	41.0	21.5	41.0
40-41	35.31158020317097	40.0	36.0	41.0	19.5	41.0
42-43	35.16974501465084	40.0	36.0	41.0	17.0	41.0
44-45	35.01459517771052	40.0	35.5	41.0	15.0	41.0
46-47	34.795127397467944	40.0	35.0	41.0	9.0	41.0
48-49	34.57261446950494	40.0	35.0	41.0	7.5	41.0
50-51	34.384799967190325	40.0	35.0	41.0	2.0	41.0
52-53	34.19815833742844	39.5	35.0	41.0	2.0	41.0
54-55	33.998882054354205	39.0	35.0	41.0	2.0	41.0
56-57	33.756228528156626	39.0	35.0	41.0	2.0	41.0
58-59	33.687149154381956	39.0	35.0	41.0	2.0	41.0
60-61	33.45024720769914	38.0	35.0	41.0	2.0	41.0
62-63	33.16234892354248	37.0	34.0	40.5	2.0	41.0
64-65	32.86187603949172	37.0	34.0	40.0	2.0	41.0
66-67	32.53946754588777	36.0	34.0	39.0	2.0	41.0
68-69	32.203744770043016	36.0	34.0	39.0	2.0	41.0
70-71	31.876938828269928	35.0	34.0	38.5	2.0	40.5
72-73	31.53628654561753	35.0	33.0	37.0	2.0	39.5
74-75	31.202775493225314	35.0	33.0	37.0	2.0	39.0
76-77	30.910104427035463	35.0	33.0	36.5	2.0	39.0
78-79	30.655357826827913	35.0	33.0	36.0	2.0	37.5
80-81	30.424609158439225	35.0	33.0	35.5	2.0	37.0
82-83	30.225293327992	35.0	33.0	35.0	2.0	37.0
84-85	30.054580867420363	35.0	32.5	35.0	2.0	36.0
86-87	29.913651240505885	35.0	32.0	35.0	2.0	36.0
88-89	29.787652943972358	35.0	32.0	35.0	2.0	36.0
90-91	29.68655740850459	35.0	32.0	35.0	2.0	35.5
92-93	29.6177435147801	35.0	32.0	35.0	2.0	35.0
94-95	29.547034642766384	35.0	32.0	35.0	2.0	35.0
96-97	29.488028863723414	35.0	32.0	35.0	2.0	35.0
98-99	29.427192553962023	35.0	32.0	35.0	2.0	35.0
100-101	29.37527637388672	35.0	32.0	35.0	2.0	35.0
102-103	28.70885663418167	34.0	30.0	35.0	2.0	35.0
104-105	29.248837563559587	35.0	31.5	35.0	2.0	35.0
106-107	29.307085717466244	35.0	32.0	35.0	2.0	35.0
108-109	29.373574372130527	35.0	32.0	35.0	2.0	35.0
110-111	29.476103133689897	35.0	32.0	35.0	2.0	35.0
112-113	29.442031973318706	35.0	32.5	35.0	2.0	35.0
114-115	29.383709484997997	35.0	32.0	35.0	2.0	35.0
116-117	29.325897084553013	35.0	32.0	35.0	2.0	35.0
118-119	29.253654287118323	35.0	32.0	35.0	2.0	35.0
120-121	29.162204282687554	35.0	32.0	35.0	2.0	35.0
122-123	29.050939945849393	35.0	32.0	35.0	2.0	35.0
124-125	28.535250257973374	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	88864.0
3	5870.0
4	4190.0
5	3912.0
6	4078.0
7	4225.0
8	4389.0
9	4472.0
10	4638.0
11	4769.0
12	5346.0
13	6570.0
14	7864.0
15	7044.0
16	4390.0
17	3848.0
18	3599.0
19	3043.0
20	2926.0
21	3100.0
22	3429.0
23	4352.0
24	5705.0
25	7388.0
26	8704.0
27	9846.0
28	11845.0
29	14156.0
30	17077.0
31	20938.0
32	27164.0
33	36370.0
34	55877.0
35	95947.0
36	219814.0
37	476664.0
38	172654.0
39	384.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.33297769783327	17.34354977133519	11.11280111452051	27.210671416311033
2	16.34800516459397	19.159969856113474	36.976207860992446	27.515817118300106
3	19.817920965307433	22.071022687741998	28.290725921325627	29.82033042562494
4	13.471198965128767	14.87012102309269	31.50131043646544	40.157369575313105
5	14.019599356176263	37.415798918059195	32.508183516175556	16.056418209588983
6	35.14627981096824	33.13851338974979	15.97252445323662	15.742682346045353
7	30.7265471284306	31.603525494051166	18.057315649214516	19.612611728303722
8	28.69049851173038	34.46398856258936	17.41810122577167	19.427411699908596
9	27.601049384343607	15.491529048493744	17.758017595204823	39.149403971957824
10-11	27.21823894304358	24.905927070593144	25.227803695539137	22.648030290824135
12-13	26.57786056381787	22.130459824019415	26.230786929836388	25.06089268232632
14-15	23.912428933736912	23.696968986803626	23.545919992735	28.84468208672446
16-17	24.130964787458502	26.91931090899637	24.326614429957573	24.623109873587552
18-19	24.20661744727566	25.87657850776044	26.11983146960235	23.796972575361547
20-21	24.82666899068513	24.740470364736634	26.245101435349934	24.187759209228307
22-23	24.679355026288018	24.291278119830004	26.105623709675413	24.92374314420657
24-25	24.25912757030461	24.88987155159724	26.031765328818096	24.819235549280055
26-27	24.034732846510053	25.063111016067218	26.217418274255174	24.684737863167555
28-29	24.107968722422115	25.356384081157067	26.109285503471014	24.4263616929498
30-31	24.621169122875884	25.056519787235132	26.039821275168425	24.282489814720556
32-33	24.276484472895767	25.094053173640063	26.017264625387508	24.61219772807666
34-35	24.3507456510706	25.23422664013575	26.25476857097032	24.160259137823328
36-37	24.37916117092448	25.24085448690579	26.27908288177313	24.1009014603966
38-39	24.276191529392122	25.400765021959774	26.26165274330606	24.061390705342045
40-41	24.223534934611347	25.444926255134746	26.147368158945287	24.184170651308616
42-43	24.120821618644683	25.38652064409488	25.93630236456673	24.556355372693712
44-45	24.039895975761855	25.453934267871936	26.13070699717529	24.37546275919092
46-47	24.055568453207034	25.485498930390033	25.70692760121015	24.752005015192783
48-49	24.049380021692464	25.744900402870552	25.60216368071795	24.603555894719033
50-51	24.05088135714866	25.785766021629485	25.68470051287084	24.478652108351014
52-53	24.232689419100357	25.76489379699455	25.581584399586653	24.42083238431844
54-55	23.922938281930293	25.40380431081013	25.865483272559764	24.807774134699816
56-57	23.935754560214903	25.690852326447448	25.59308243210485	24.780310681232795
58-59	23.98577466346284	25.718608723418125	25.781628194640454	24.513988418478583
60-61	24.288678246235126	25.50589512183154	25.88082618856334	24.32460044336999
62-63	24.166740512841546	25.517210064659956	25.869254920169233	24.446794502329265
64-65	23.99749240360877	25.24781189511744	25.887087855953823	24.867607845319974
66-67	23.94622729047033	25.640319572068133	25.65423438849142	24.75921874897012
68-69	23.773573713007643	25.56741325759767	25.87251391664732	24.78649911274736
70-71	23.789539133956474	25.38168707628469	25.896132486628957	24.93264130312988
72-73	23.865997388408665	25.64365180442213	25.632153771903937	24.858197035265274
74-75	23.658776477515488	25.68147813433071	25.770569577377735	24.889175810776074
76-77	23.73640650598227	25.337049809916284	25.762440395151494	25.164103288949953
78-79	23.637391601749165	25.43467323250706	25.906348891318693	25.02158627442508
80-81	23.8003414256535	25.586125023893203	25.569427244185256	25.044106306268038
82-83	23.87039154096339	25.567340021721762	25.654380860243247	24.907887577071605
84-85	23.83791142999639	25.656065285389225	25.48403421287179	25.021989071742595
86-87	23.488576301895858	25.81787995321692	25.647240362341822	25.0463033825454
88-89	23.459172097717165	25.57437066580932	25.687446858217537	25.279010378255972
90-91	23.665482316590214	25.36759953612243	25.78669321720038	25.18022493008697
92-93	23.67456165373254	25.4704969700343	25.826058840179883	25.028882536053278
94-95	23.66818533683792	25.36550248306601	25.72421232869188	25.24209985140419
96-97	23.373963570104827	25.373458972517742	25.762855978401173	25.489721478976264
98-99	23.317542452106466	25.43933302598666	25.65493952362242	25.588184998284447
100-101	23.371545372188383	25.425738455645792	25.78331261978643	25.4194035523794
102-103	23.171098779817072	25.360448672270188	25.84761371883722	25.62083882907552
104-105	23.24093769785137	25.407804969861598	25.770066362713255	25.58119096957378
106-107	23.256937189502203	25.313221260267234	25.772850459025005	25.656991091205562
108-109	23.168059924808368	25.309853311504053	25.794091228167826	25.727995535519753
110-111	23.101899455415236	25.588343425288773	25.74243249814712	25.567324621148874
112-113	23.03840918852986	25.60325726147916	25.761374617660294	25.596958932330676
114-115	23.022502928533527	25.628650596930157	25.70287551050226	25.645970964034053
116-117	22.811754134006325	25.43167309071122	25.757391557172287	25.999181218110166
118-119	22.92502855293122	25.281730188623282	25.8216268451382	25.971614413307297
120-121	22.75732946256467	25.36888991744834	25.969754981515226	25.90402563847177
122-123	22.66487283981856	25.514171976679762	25.75655098949154	26.064404194010137
124-125	22.468571704775606	25.377430655619264	25.730098403492768	26.42389923611236
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	39.0
1	43.5
2	52.5
3	51.5
4	52.0
5	58.5
6	141.0
7	341.5
8	484.5
9	593.5
10	588.5
11	511.5
12	545.0
13	577.5
14	672.5
15	743.0
16	808.5
17	894.0
18	917.0
19	981.5
20	1048.5
21	1127.0
22	1223.5
23	1366.5
24	1568.5
25	1847.5
26	2372.0
27	3043.0
28	3738.0
29	4650.5
30	5872.0
31	7246.5
32	9041.5
33	11152.0
34	13286.5
35	15583.5
36	18368.0
37	21293.5
38	23787.5
39	26358.0
40	29324.5
41	31794.5
42	34347.0
43	38071.5
44	42074.0
45	46337.5
46	49576.0
47	52944.0
48	57299.0
49	60564.0
50	61912.0
51	62460.5
52	62822.5
53	62312.0
54	61943.5
55	60271.0
56	56269.0
57	51612.0
58	47517.0
59	43012.5
60	36641.0
61	30345.0
62	25017.0
63	19285.0
64	14175.0
65	10749.0
66	8184.5
67	6050.0
68	4466.0
69	3350.0
70	2628.5
71	2011.5
72	1498.0
73	1182.0
74	931.5
75	681.5
76	490.0
77	340.5
78	245.0
79	196.0
80	151.5
81	133.5
82	132.5
83	121.0
84	104.0
85	100.0
86	90.0
87	76.0
88	67.0
89	66.5
90	69.0
91	59.0
92	57.0
93	55.0
94	51.0
95	48.0
96	32.5
97	24.5
98	21.0
99	7.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33007409273566024
2	0.0
3	0.0
4	0.01882162010939975
5	0.1717381290137837
6	0.044014761423148835
7	0.02262988565682694
8	0.007836238722590557
9	0.0057856341970528415
10-11	0.001537953394153287
12-13	0.05529308631360627
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	3.295614416042758E-4
92-93	4.760331934283984E-4
94-95	1.4647175182412258E-4
96-97	4.760331934283984E-4
98-99	3.6617937956030646E-5
100-101	0.0
102-103	0.0
104-105	3.6617937956030646E-5
106-107	1.830896897801532E-4
108-109	4.760331934283984E-4
110-111	5.12651131384429E-4
112-113	6.957408211645823E-4
114-115	4.760331934283984E-4
116-117	5.858870072964903E-4
118-119	3.295614416042758E-4
120-121	2.197076277361839E-4
122-123	0.0020139865875816857
124-125	1.0985381386809195E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1365451.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.48049000119578
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.72099650496217	55.65812871038284
2	8.93045326921631	11.87401818540562
3	2.9186296008846853	5.820957779964249
4	1.3573053294790038	3.609372935399946
5	0.7765362789607096	2.581225616450661
6	0.5014717423424033	2.0002852291605824
7	0.34039931218388614	1.5840939149038331
8	0.2576616277148875	1.3703577011993122
9	0.18896141705114133	1.1306022837192167
>10	0.9547483373906632	10.970416722027018
>50	0.04127766472054914	1.8126739142759924
>100	0.011008510717488184	1.1622387198036173
>500	2.2016175044459394E-4	0.09799110672357443
>1k	3.302426256668909E-4	0.3276371805836018
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1896	0.1388552207292682	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1433	0.10494701018198382	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.323587591206129E-5
2	0.0	0.0	0.0	0.0	7.323587591206129E-5
3	0.0	0.0	0.0	0.0	7.323587591206129E-5
4	0.0	0.0	0.0	0.0	7.323587591206129E-5
5	0.0	0.0	0.0	0.0	7.323587591206129E-5
6	0.0	0.0	0.0	0.0	7.323587591206129E-5
7	0.0	0.0	0.0	0.0	7.323587591206129E-5
8	0.0	0.0	0.0	0.0	1.4647175182412258E-4
9	0.0	0.0	0.0	0.0	2.1970762773618386E-4
10-11	0.0	0.0	0.0	0.0	2.1970762773618386E-4
12-13	0.0	0.0	0.0	0.0	2.1970762773618386E-4
14-15	0.0	0.0	0.0	0.0	3.661793795603064E-4
16-17	0.0	0.0	0.0	0.0	4.3941525547236773E-4
18-19	0.0	0.0	0.0	0.0	5.12651131384429E-4
20-21	0.0	0.0	0.0	1.4647175182412258E-4	5.492690693404596E-4
22-23	0.0	0.0	0.0	2.9294350364824517E-4	7.32358759120613E-4
24-25	0.0	0.0	0.0	6.957408211645822E-4	8.788305109447355E-4
26-27	0.0	0.0	0.0	0.0012816278284610726	8.788305109447355E-4
28-29	0.0	0.0	0.0	0.0036251758576470337	8.788305109447355E-4
30-31	0.0	0.0	0.0	0.013804962609423554	8.788305109447355E-4
32-33	0.0	0.0	0.0	0.03068583200715368	9.886843248128273E-4
34-35	0.0	0.0	0.0	0.049763777682245644	0.0010985381386809194
36-37	0.0	0.0	0.0	0.0799369585580149	0.0010985381386809194
38-39	0.0	0.0	0.0	0.13021338737164498	0.00113515607663695
40-41	0.0	0.0	0.0	0.1893879751085905	0.0011717740145929807
42-43	0.0	0.0	0.0	0.24215442370323065	0.0012083919525490113
44-45	0.0	0.0	0.0	0.29821648671391354	0.001245009890505042
46-47	0.0	0.0	0.0	0.3660329078084823	0.001245009890505042
48-49	0.0	0.0	0.0	0.43377609302713904	0.0013182457664171032
50-51	0.0	0.0	0.0	0.5074513841946726	0.0013914816423291644
52-53	0.0	0.0	0.0	0.5805407883549099	0.0013914816423291644
54-55	0.0	0.0	0.0	0.6661169093581535	0.001428099580285195
56-57	0.0	0.0	0.0	0.7556111497226923	0.0014647175182412257
58-59	0.0	0.0	0.0	0.8546992898317113	0.0014647175182412257
60-61	0.0	0.0	0.0	0.9564971573494765	0.0014647175182412257
62-63	0.0	0.0	0.0	1.069500113881787	0.0014647175182412257
64-65	0.0	0.0	0.0	1.181990419282713	0.0014647175182412257
66-67	0.0	0.0	0.0	1.308212451417151	0.0015379533941532872
68-69	0.0	0.0	0.0	1.4447607420551891	0.0015379533941532872
70-71	0.0	0.0	0.0	1.5899508660508506	0.0016111892700653484
72-73	0.0	0.0	0.0	1.7307102195538324	0.001940750711669624
74-75	0.0	0.0	0.0	1.8711033936772539	0.0019773686496256547
76-77	0.0	0.0	0.0	2.046063901231168	0.0019773686496256547
78-79	0.0	0.0	0.0	2.2362208530368353	0.0019773686496256547
80-81	0.0	0.0	0.0	2.4334816848059724	0.002050604525537716
82-83	0.0	0.0	0.0	2.6303397192575932	0.0023069300912299307
84-85	0.0	0.0	0.0	2.84817983215802	0.0023435480291859614
86-87	0.0	0.0	0.0	3.0820586018831873	0.0023435480291859614
88-89	0.0	0.0	0.0	3.3481611570096623	0.0023435480291859614
90-91	0.0	0.0	0.0	3.633012096369624	0.0023435480291859614
92-93	0.0	0.0	0.0	3.9162884643974776	0.0024167839050980226
94-95	0.0	0.0	0.0	4.194511556987399	0.0024167839050980226
96-97	0.0	0.0	0.0	4.503640189212209	0.0024534018430540532
98-99	0.0	0.0	0.0	4.854586506582807	0.002490019781010084
100-101	0.0	0.0	0.0	5.233508928551812	0.002490019781010084
102-103	0.0	0.0	0.0	5.625870133750681	0.002563255656922145
104-105	0.0	0.0	0.0	6.0182313389495485	0.002563255656922145
106-107	0.0	0.0	0.0	6.438495412870912	0.002563255656922145
108-109	0.0	0.0	0.0	6.891276215697231	0.0026364915328342064
110-111	0.0	0.0	0.0	7.388950610457643	0.00285619916057039
112-113	0.0	0.0	0.0	7.890433270765483	0.0029294350364824514
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1885	0.0	75.463776	1
GTATCAA	3100	0.0	46.145126	2
GTACATG	6355	0.0	41.287815	1
TACATGG	6650	0.0	39.526943	2
TCAACGC	3780	0.0	38.78998	5
ACATGGG	6735	0.0	38.408592	3
CAACGCA	3890	0.0	37.363342	6
AACGCAG	4000	0.0	36.32253	7
ATCAACG	4020	0.0	36.029358	4
ATGGGGG	3475	0.0	34.99062	5
CATGGGG	6030	0.0	34.59609	4
TATCAAC	4235	0.0	34.059498	3
ACGCAGA	4915	0.0	29.315035	8
CGCAGAG	5195	0.0	27.620405	9
TATACCG	155	6.74036E-6	26.91799	5
GAGTACT	2785	0.0	25.32309	12-13
ATACCGT	195	1.4508605E-6	24.43773	6
GTACTTT	3030	0.0	23.560642	14-15
GTGGTAT	990	0.0	23.545197	1
GAGTACA	4310	0.0	23.297266	1
>>END_MODULE