FastQCFastQC Report
Mon 15 Aug 2016
SRR2921287_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921287_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1832157
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16850.060.7476841
GTATCAA29600.053.479961
TCAACGC36100.042.3646244
GTACATG87950.041.0051081
CAACGCA37500.040.6243255
TATCAAC39400.039.4204642
AACGCAG38500.039.4124266
TACATGG90150.039.144112
ACATGGG91400.038.608773
ATCAACG40600.037.6690373
CATGGGG84650.034.5169144
ATGGGGG54550.031.9632055
ACGCAGA48950.030.8702347
CGCAGAG51200.029.3974368
TGGGGGG36000.025.6202246
GCAGAGT60150.024.8240859
GTATAGT4200.022.671061
GAGTACA59100.022.1531941
CAGAGTA57150.022.1193310-11
AGAGTAC53450.022.09235610-11
Produced by FastQC (version 0.11.5)