FastQCFastQC Report
Mon 15 Aug 2016
SRR2921287_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921287_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1832157
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20160.11003423833219532No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19950.068.90941
GTATCAA32500.049.8401641
GTACATG82450.041.7566221
TACATGG85450.039.54832
ACATGGG87100.038.5938223
CATGGGG79600.035.4913144
TCAACGC44750.035.1615264
TATCAAC47050.034.076072
CAACGCA46650.033.6016775
ATGGGGG49300.033.2462465
ATCAACG48300.032.577193
AACGCAG47650.032.009556
GTGGTAT10200.027.541731
ACGCAGA60550.024.791677
CGCAGAG63450.023.4663098
TGGTATC11100.023.088852
TGGGGGG36700.022.7281686
GAGTACA57400.021.6593281
AGTACAT55400.021.516772
ATGGGGA32250.021.2532315
Produced by FastQC (version 0.11.5)