##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921287_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1832157
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.243039215525744	33.0	30.0	34.0	19.0	34.0
2	30.000829077420768	33.0	31.0	34.0	26.0	34.0
3	30.2275536430557	33.0	31.0	34.0	26.0	34.0
4	32.423827761485505	37.0	35.0	37.0	19.0	37.0
5	32.04967205321378	37.0	33.0	37.0	19.0	37.0
6	32.18147298512082	37.0	35.0	37.0	17.0	37.0
7	32.3996251412952	37.0	35.0	37.0	17.0	37.0
8	32.64647243658704	37.0	35.0	37.0	17.0	37.0
9	34.39867162039061	39.0	37.0	39.0	17.0	39.0
10-11	34.57516713906068	39.0	37.0	39.0	23.0	39.0
12-13	34.59964484484681	39.0	37.0	39.0	24.5	39.0
14-15	36.02505080077744	40.5	38.0	41.0	25.0	41.0
16-17	36.04513778022299	41.0	38.0	41.0	25.0	41.0
18-19	36.067551252430874	41.0	38.0	41.0	25.5	41.0
20-21	36.05151960230482	41.0	38.0	41.0	25.0	41.0
22-23	36.00685940124127	41.0	38.0	41.0	25.0	41.0
24-25	35.957532842436535	41.0	38.0	41.0	25.0	41.0
26-27	35.86191412635489	41.0	38.0	41.0	24.0	41.0
28-29	35.783578044894625	40.0	38.0	41.0	24.0	41.0
30-31	35.690316932446294	40.0	37.0	41.0	23.5	41.0
32-33	35.56753133055737	40.0	37.0	41.0	22.0	41.0
34-35	35.486313399997925	40.0	37.0	41.0	21.5	41.0
36-37	35.40230422392841	40.0	36.5	41.0	19.5	41.0
38-39	35.3039635795404	40.0	36.0	41.0	18.5	41.0
40-41	35.173873472633616	40.0	36.0	41.0	16.5	41.0
42-43	35.03019146284953	40.0	35.5	41.0	15.0	41.0
44-45	34.880273906657564	40.0	35.0	41.0	11.0	41.0
46-47	34.675076153408256	40.0	35.0	41.0	8.0	41.0
48-49	34.46510697500268	40.0	35.0	41.0	4.0	41.0
50-51	34.28088831906872	40.0	35.0	41.0	2.0	41.0
52-53	34.084901021036956	39.0	35.0	41.0	2.0	41.0
54-55	33.88121460115045	39.0	35.0	41.0	2.0	41.0
56-57	33.635451274099324	39.0	34.5	41.0	2.0	41.0
58-59	33.561965213679834	38.5	35.0	41.0	2.0	41.0
60-61	33.32148118310822	37.5	34.0	41.0	2.0	41.0
62-63	33.035474579962305	37.0	34.0	40.0	2.0	41.0
64-65	32.732341715256936	36.5	34.0	40.0	2.0	41.0
66-67	32.41546548685511	36.0	34.0	39.0	2.0	41.0
68-69	32.08197850948363	35.5	34.0	39.0	2.0	41.0
70-71	31.755861260798063	35.0	33.5	38.5	2.0	40.5
72-73	31.414998550888377	35.0	33.0	37.0	2.0	39.5
74-75	31.091899056685644	35.0	33.0	37.0	2.0	39.0
76-77	30.80243259720646	35.0	33.0	36.0	2.0	39.0
78-79	30.55292477664305	35.0	33.0	36.0	2.0	37.5
80-81	30.32950123815808	35.0	33.0	35.5	2.0	37.0
82-83	30.13694896234329	35.0	32.5	35.0	2.0	37.0
84-85	29.969207606116726	35.0	32.0	35.0	2.0	36.0
86-87	29.828804518390072	35.0	32.0	35.0	2.0	36.0
88-89	29.70864969541366	35.0	32.0	35.0	2.0	36.0
90-91	29.61233944470916	35.0	32.0	35.0	2.0	35.0
92-93	29.54431416084975	35.0	32.0	35.0	2.0	35.0
94-95	29.477653115972046	35.0	32.0	35.0	2.0	35.0
96-97	29.413239149265046	35.0	32.0	35.0	2.0	35.0
98-99	29.355382207965803	35.0	32.0	35.0	2.0	35.0
100-101	29.304072194686373	35.0	32.0	35.0	2.0	35.0
102-103	28.626988571394264	34.0	30.0	35.0	2.0	35.0
104-105	29.171306007072538	35.0	31.0	35.0	2.0	35.0
106-107	29.23108909334735	35.0	32.0	35.0	2.0	35.0
108-109	29.303424870248563	35.0	32.0	35.0	2.0	35.0
110-111	29.41099070658246	35.0	32.0	35.0	2.0	35.0
112-113	29.374321905819208	35.0	32.0	35.0	2.0	35.0
114-115	29.313575201251858	35.0	32.0	35.0	2.0	35.0
116-117	29.258208767043435	35.0	32.0	35.0	2.0	35.0
118-119	29.183037534447102	35.0	32.0	35.0	2.0	35.0
120-121	29.085477390856788	35.0	32.0	35.0	2.0	35.0
122-123	28.974997502943253	35.0	32.0	35.0	2.0	35.0
124-125	28.454571851648083	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	123539.0
3	7794.0
4	5873.0
5	5651.0
6	5661.0
7	5934.0
8	6194.0
9	6199.0
10	6235.0
11	6432.0
12	7018.0
13	8323.0
14	9577.0
15	8323.0
16	5712.0
17	4934.0
18	4528.0
19	4022.0
20	3912.0
21	4077.0
22	4605.0
23	5900.0
24	7823.0
25	10008.0
26	11886.0
27	13838.0
28	16197.0
29	19376.0
30	23403.0
31	29003.0
32	37510.0
33	50212.0
34	76000.0
35	132100.0
36	303106.0
37	631540.0
38	219372.0
39	340.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.70107034476714	17.44437384283351	11.390736094830137	27.46381971756921
2	16.220225668433436	19.149286878799142	37.24375149072923	27.386735962038188
3	19.271219660760515	22.45408008156506	28.339274418076617	29.935425839597805
4	13.586309150124848	15.262672901624722	31.234585019723532	39.916432928526895
5	14.394233513818547	37.12645739714147	32.26806592832138	16.211243160718602
6	35.119103528872095	33.136884413329106	16.048886510337596	15.6951255474612
7	30.613078297594953	31.345461652647742	18.495313893009246	19.546146156748055
8	28.2845728203513	34.49293415160965	17.679495584288265	19.54299744375078
9	27.674622344127396	15.435870143693453	17.843752132203434	39.04575537997571
10-11	27.0714418854966	24.717354811647656	25.397198805858707	22.814004496997047
12-13	26.6255679920748	22.108309033562218	26.20820082119073	25.057922153172253
14-15	23.97117168452267	23.682522840564427	23.546153522869492	28.80015195204341
16-17	24.350997212575123	26.653256243869933	24.300892336191712	24.694854207363235
18-19	24.476395854722057	25.705711901327234	25.88413547528951	23.9337567686612
20-21	25.127158862477394	24.65340033632489	25.79953573847656	24.419905062721153
22-23	25.039038685003522	24.180924451343415	25.686281252097938	25.093755611555125
24-25	24.59549045196454	24.784584508860323	25.57346340952222	25.046461629652917
26-27	24.27090036497964	24.9415033755295	25.82284160145664	24.96475465803422
28-29	24.400119640401996	25.21710748587594	25.67334567943686	24.709427194285205
30-31	24.887059351354715	24.914049396421813	25.5648942748902	24.633996977333275
32-33	24.522734678305408	25.017206494858247	25.569506325058388	24.89055250177796
34-35	24.695863946157452	25.084176738128882	25.79009331623873	24.429865999474938
36-37	24.72522824190285	25.080274234140415	25.760729020493333	24.433768503463405
38-39	24.598819860961697	25.337157241437282	25.726807255055107	24.337215642545917
40-41	24.58637551257889	25.247317778989466	25.66051926772651	24.505787440705138
42-43	24.4699553586292	25.177072707197034	25.46970592585679	24.883266008316973
44-45	24.339944666314075	25.27769181352908	25.66955233639912	24.712811183757726
46-47	24.38532833157857	25.325831792799413	25.307956687117972	24.98088318850404
48-49	24.42058731866319	25.55621597930745	25.172788139881025	24.85040856214833
50-51	24.32717283507909	25.60356454168502	25.35942607538546	24.70983654785043
52-53	24.583400876671597	25.44555406550858	25.3139878296456	24.657057228174224
54-55	24.22423405854411	25.197922448785775	25.518500870831485	25.05934262183863
56-57	24.250323525767715	25.42917992289962	25.224039206247063	25.0964573450856
58-59	24.36030318362455	25.480894923306245	25.337102660961914	24.821699232107292
60-61	24.609926987698106	25.257742649783832	25.5533232141132	24.57900714840486
62-63	24.36843567445366	25.32075580859064	25.585525694577484	24.725282822378215
64-65	24.313909779565833	25.067284081003976	25.45865337959574	25.160152759834446
66-67	24.28798405376832	25.487499160825188	25.258534066676603	24.96598271872989
68-69	24.116792392791666	25.349001204591094	25.489191155561453	25.04501524705579
70-71	24.123287469359887	25.216889163974486	25.61109664728514	25.048726719380486
72-73	24.28187104052764	25.53634868627525	25.192600852437863	24.989179420759246
74-75	24.046683772187645	25.536676169127425	25.332845383883583	25.08379467480134
76-77	24.13084686519769	25.17625400006659	25.33598376121697	25.356915373518753
78-79	24.02627067440181	25.231025507093552	25.463156268813208	25.27954754969143
80-81	24.171482029105583	25.55163121937694	25.13935759872107	25.1375291527964
82-83	24.16727933250262	25.499233963028278	25.276300011407322	25.057186693061784
84-85	24.14219960407323	25.4501115352014	25.12104584923672	25.286643011488646
86-87	23.813270369296955	25.72066695157675	25.200051087324937	25.26601159180136
88-89	23.82909870715228	25.431772495479372	25.254658852925814	25.484469944442534
90-91	23.99923150585827	25.30580799817919	25.328786409661415	25.36617408630113
92-93	23.92794696723877	25.506474495408536	25.3287598371422	25.23681870021049
94-95	23.960780642919968	25.291916929585433	25.272240662847306	25.47506176464729
96-97	23.78936664864595	25.239465862657024	25.264163615379847	25.707003873317174
98-99	23.633618734639008	25.353176610956375	25.210721570258443	25.80248308414617
100-101	23.681949745573114	25.487608321775916	25.265957011325995	25.564484921324976
102-103	23.49566658315854	25.32040103550078	25.396677249820836	25.787255131519842
104-105	23.550465380423187	25.404973482076045	25.29428427803949	25.75027685946128
106-107	23.58742279930781	25.341721626946168	25.228494399905248	25.84236117384077
108-109	23.5804591380457	25.29470149968575	25.323984028604634	25.800855333663907
110-111	23.42296661764413	25.61664557578649	25.279473493034548	25.68091431353483
112-113	23.419012458614105	25.687226891306697	25.240046371850276	25.653714278228918
114-115	23.358134741948547	25.70015948441015	25.142646305932796	25.799059467708503
116-117	23.19192467204487	25.57464637828211	25.26877597530986	25.96465297436316
118-119	23.282950014027204	25.48958766566566	25.207815500225415	26.019646820081714
120-121	23.148196916906798	25.545157651910717	25.344219248005146	25.96242618317734
122-123	23.11924472319817	25.72785779120725	25.114143537306173	26.038753948288406
124-125	22.915576761906944	25.562049727766013	25.113152240625862	26.409221269701177
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	47.0
1	42.5
2	54.5
3	67.0
4	69.0
5	82.5
6	176.0
7	411.5
8	594.5
9	703.0
10	681.0
11	584.0
12	595.0
13	610.5
14	666.5
15	759.5
16	851.0
17	941.0
18	984.5
19	1007.0
20	1121.0
21	1246.0
22	1314.0
23	1482.0
24	1693.5
25	1985.0
26	2525.5
27	3259.0
28	4017.0
29	5145.5
30	6876.0
31	8742.5
32	11308.5
33	14007.0
34	16441.0
35	19391.5
36	22856.5
37	26770.5
38	30619.0
39	34337.0
40	38085.5
41	41540.0
42	45388.0
43	50694.0
44	56050.5
45	61405.0
46	65994.0
47	70131.5
48	75795.5
49	80564.0
50	82582.0
51	84087.5
52	84749.5
53	83875.5
54	83461.5
55	81814.0
56	77022.0
57	70569.0
58	65089.5
59	59072.5
60	50692.5
61	42628.5
62	35535.5
63	27934.5
64	20962.0
65	15970.0
66	12168.0
67	9323.0
68	7427.5
69	5808.5
70	4593.5
71	3513.0
72	2611.0
73	2141.5
74	1687.0
75	1346.0
76	1060.0
77	713.5
78	479.0
79	354.0
80	273.5
81	213.5
82	185.0
83	161.5
84	149.0
85	137.5
86	127.5
87	116.0
88	106.0
89	101.5
90	90.5
91	79.5
92	79.5
93	77.5
94	67.5
95	68.0
96	63.0
97	50.5
98	32.5
99	13.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35897578646371464
2	0.0
3	0.0
4	0.018284459246669365
5	0.18464574815367898
6	0.05332512442983871
7	0.025434501519247532
8	0.009606163663921814
9	0.007204622747941361
10-11	0.0024834116290252417
12-13	0.05651808223858545
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	1.3645118840798032E-4
92-93	3.5477308986074885E-4
94-95	2.7290237681596065E-5
96-97	3.5477308986074885E-4
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0
106-107	2.7290237681596065E-5
108-109	3.5477308986074885E-4
110-111	3.5477308986074885E-4
112-113	6.003852289951134E-4
114-115	1.637414260895764E-4
116-117	4.6393404058713304E-4
118-119	1.637414260895764E-4
120-121	1.9103166377117245E-4
122-123	0.0018557361623485322
124-125	8.18707130447882E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1832157.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.47570708398603
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.26672676963011	52.21938651187352
2	9.456769437021984	12.00550253490398
3	3.1932959065160262	6.080901467835088
4	1.57251481590512	3.992659593584865
5	0.863946662830995	2.7419812653023734
6	0.5778370438432797	2.200716896236345
7	0.3851171813958959	1.7111909779507342
8	0.28934647301860134	1.4693177573690563
9	0.2106310208494636	1.2032957684017358
>10	1.1182149233071461	12.353885952573389
>50	0.05035001071904027	2.1677651195900824
>100	0.014646915586807868	1.4469602264540118
>500	3.44479643176883E-4	0.14125493446642934
>1k	2.583597323826621E-4	0.26518099345845525
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2016	0.11003423833219532	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.458047536319213E-5	0.0
5	0.0	0.0	0.0	5.458047536319213E-5	0.0
6	0.0	0.0	0.0	5.458047536319213E-5	0.0
7	0.0	0.0	0.0	5.458047536319213E-5	0.0
8	0.0	0.0	0.0	5.458047536319213E-5	0.0
9	0.0	0.0	0.0	5.458047536319213E-5	0.0
10-11	0.0	0.0	0.0	5.458047536319213E-5	0.0
12-13	0.0	0.0	0.0	5.458047536319213E-5	1.637414260895764E-4
14-15	0.0	0.0	0.0	5.458047536319213E-5	2.456121391343646E-4
16-17	0.0	0.0	0.0	8.18707130447882E-5	2.7290237681596065E-4
18-19	0.0	0.0	0.0	1.0916095072638426E-4	2.7290237681596065E-4
20-21	0.0	0.0	0.0	1.0916095072638426E-4	2.7290237681596065E-4
22-23	0.0	0.0	0.0	2.1832190145276852E-4	2.7290237681596065E-4
24-25	0.0	0.0	0.0	4.3664380290553705E-4	3.2748285217915274E-4
26-27	0.0	0.0	0.0	7.095461797214976E-4	3.2748285217915274E-4
28-29	2.7290237681596065E-5	0.0	0.0	0.002892765194249183	3.2748285217915274E-4
30-31	5.458047536319213E-5	0.0	0.0	0.011762092440767903	3.2748285217915274E-4
32-33	5.458047536319213E-5	0.0	0.0	0.028491008139586292	3.2748285217915274E-4
34-35	5.458047536319213E-5	0.0	0.0	0.05005029590804719	3.820633275423449E-4
36-37	5.458047536319213E-5	0.0	0.0	0.07944188189112614	3.820633275423449E-4
38-39	5.458047536319213E-5	0.0	0.0	0.1282641171035015	4.09353565223941E-4
40-41	5.458047536319213E-5	0.0	0.0	0.1837724605478679	4.3664380290553705E-4
42-43	5.458047536319213E-5	0.0	0.0	0.23565120238058201	4.3664380290553705E-4
44-45	5.458047536319213E-5	0.0	0.0	0.29421605244528715	4.912242782687292E-4
46-47	5.458047536319213E-5	0.0	0.0	0.3627418392637749	4.912242782687292E-4
48-49	5.458047536319213E-5	0.0	0.0	0.43304149153156635	4.912242782687292E-4
50-51	5.458047536319213E-5	0.0	0.0	0.5060701675675174	4.912242782687292E-4
52-53	5.458047536319213E-5	0.0	0.0	0.5838473449600662	6.003852289951134E-4
54-55	5.458047536319213E-5	0.0	0.0	0.6745600950136915	7.095461797214977E-4
56-57	5.458047536319213E-5	0.0	0.0	0.7677835469340237	7.095461797214977E-4
58-59	5.458047536319213E-5	0.0	0.0	0.8669289804312621	7.095461797214977E-4
60-61	5.458047536319213E-5	0.0	0.0	0.9733336171518052	7.641266550846898E-4
62-63	5.458047536319213E-5	0.0	0.0	1.0858239768753442	8.732876058110741E-4
64-65	8.18707130447882E-5	0.0	0.0	1.2010706506047244	8.732876058110741E-4
66-67	1.0916095072638426E-4	0.0	0.0	1.3330189497952414	8.732876058110741E-4
68-69	1.0916095072638426E-4	0.0	0.0	1.4773843071308845	8.732876058110741E-4
70-71	1.0916095072638426E-4	0.0	0.0	1.6272077120028468	8.732876058110741E-4
72-73	1.0916095072638426E-4	0.0	0.0	1.7741656419182417	9.278680811742662E-4
74-75	1.0916095072638426E-4	0.0	0.0	1.9261995560424134	9.278680811742662E-4
76-77	1.0916095072638426E-4	0.0	0.0	2.1079798292395244	9.278680811742662E-4
78-79	1.0916095072638426E-4	0.0	0.0	2.3135026092196247	9.278680811742662E-4
80-81	1.0916095072638426E-4	0.0	0.0	2.5138948245155848	9.551583188558623E-4
82-83	1.0916095072638426E-4	0.0	0.0	2.7217645649363016	0.0010916095072638426
84-85	1.0916095072638426E-4	0.0	0.0	2.934710289565796	0.0010916095072638426
86-87	1.0916095072638426E-4	0.0	0.0	3.178139209685633	0.0010916095072638426
88-89	1.0916095072638426E-4	0.0	0.0	3.4524879690987182	0.0010916095072638426
90-91	1.0916095072638426E-4	0.0	0.0	3.7447118341932484	0.0010916095072638426
92-93	1.0916095072638426E-4	0.0	0.0	4.031041007948555	0.0010916095072638426
94-95	1.0916095072638426E-4	0.0	0.0	4.3211635247416025	0.0010916095072638426
96-97	1.0916095072638426E-4	0.0	0.0	4.645944643390277	0.0011461899826270346
98-99	1.0916095072638426E-4	0.0	0.0	5.01258898664252	0.0012007704579902268
100-101	1.0916095072638426E-4	0.0	0.0	5.406960211379265	0.001255350933353419
102-103	1.0916095072638426E-4	0.0	0.0	5.810446375501662	0.001255350933353419
104-105	1.0916095072638426E-4	0.0	0.0	6.221792128076361	0.001255350933353419
106-107	1.0916095072638426E-4	0.0	0.0	6.667059646089282	0.0012826411710350149
108-109	1.0916095072638426E-4	0.0	0.0	7.14390196909981	0.001337221646398207
110-111	1.0916095072638426E-4	0.0	0.0	7.659469139380523	0.0013918021217613993
112-113	1.0916095072638426E-4	0.0	0.0	8.168050008814747	0.0014190923594429954
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1995	0.0	68.9094	1
GTATCAA	3250	0.0	49.840164	1
GTACATG	8245	0.0	41.756622	1
TACATGG	8545	0.0	39.5483	2
ACATGGG	8710	0.0	38.593822	3
CATGGGG	7960	0.0	35.491314	4
TCAACGC	4475	0.0	35.161526	4
TATCAAC	4705	0.0	34.07607	2
CAACGCA	4665	0.0	33.601677	5
ATGGGGG	4930	0.0	33.246246	5
ATCAACG	4830	0.0	32.57719	3
AACGCAG	4765	0.0	32.00955	6
GTGGTAT	1020	0.0	27.54173	1
ACGCAGA	6055	0.0	24.79167	7
CGCAGAG	6345	0.0	23.466309	8
TGGTATC	1110	0.0	23.08885	2
TGGGGGG	3670	0.0	22.728168	6
GAGTACA	5740	0.0	21.659328	1
AGTACAT	5540	0.0	21.51677	2
ATGGGGA	3225	0.0	21.253231	5
>>END_MODULE