FastQCFastQC Report
Mon 15 Aug 2016
SRR2921286_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921286_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1510204
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22200.056.8290251
GGTATCA12950.054.6845321
TCAACGC28850.042.903284
CAACGCA29050.042.60795
AACGCAG30450.040.452156
GTACATG75250.040.0948641
TATCAAC31550.039.420292
TACATGG76200.038.7346532
ATCAACG32000.038.494023
ACATGGG76900.037.685613
CATGGGG69650.033.748074
ATGGGGG42200.032.57415
ACGCAGA39550.031.1383847
CGCAGAG44100.027.790798
TAGTACG1505.290516E-627.7702472
GCAGAGT49150.024.6908349
AGAGTAC43050.022.31662210-11
GAGTACA51000.022.1702561
AGTACAT49250.022.1114772
TATTACG1350.002553739422.0398772
Produced by FastQC (version 0.11.5)