FastQCFastQC Report
Mon 15 Aug 2016
SRR2921286_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921286_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1510204
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17500.061.4631271
GTATCAA26450.047.2172661
GTACATG70100.039.553071
TACATGG72050.038.2990262
ACATGGG73150.037.560153
CATGGGG67000.035.0479134
ATGGGGG40150.033.1024445
TCAACGC35300.032.923094
TATCAAC38500.032.6634832
CAACGCA35500.032.401845
ATCAACG37500.031.627333
AACGCAG37100.030.9900656
TGGGGGG27700.024.3017626
ACGCAGA47750.023.8207347
GTGGTAT11050.022.7126221
TACGTAA1903.2642587E-521.9576155
GAGTACA49100.021.6630061
TTACGTA1953.9884733E-521.3945984
CGCAGAG53550.021.2378968
AGTACAT48800.020.7620322
Produced by FastQC (version 0.11.5)