##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921286_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1510204
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.33360857208695	33.0	30.0	34.0	20.0	34.0
2	30.07240611202195	33.0	31.0	34.0	26.0	34.0
3	30.300683881118047	33.0	31.0	34.0	27.0	34.0
4	32.55175393522994	37.0	35.0	37.0	19.0	37.0
5	32.17722771228258	37.0	33.0	37.0	19.0	37.0
6	32.312433949320756	37.0	35.0	37.0	17.0	37.0
7	32.52067866327993	37.0	35.0	37.0	17.0	37.0
8	32.75905970319241	37.0	35.0	37.0	17.0	37.0
9	34.51912854157452	39.0	37.0	39.0	18.0	39.0
10-11	34.67825936098699	39.0	37.0	39.0	25.0	39.0
12-13	34.697981199890876	39.0	37.0	39.0	25.0	39.0
14-15	36.12377930398807	41.0	38.0	41.0	27.0	41.0
16-17	36.137576777706855	41.0	38.0	41.0	27.0	41.0
18-19	36.15209335957262	41.0	38.0	41.0	27.0	41.0
20-21	36.139932088644976	41.0	38.0	41.0	26.0	41.0
22-23	36.096600194410826	41.0	38.0	41.0	26.0	41.0
24-25	36.04575209706768	41.0	38.0	41.0	25.5	41.0
26-27	35.95108409195049	40.5	38.0	41.0	25.0	41.0
28-29	35.87402198643362	40.0	38.0	41.0	24.5	41.0
30-31	35.78683575199112	40.0	37.5	41.0	24.0	41.0
32-33	35.66731911715239	40.0	37.0	41.0	24.0	41.0
34-35	35.58286430177645	40.0	37.0	41.0	23.0	41.0
36-37	35.50282908799076	40.0	37.0	41.0	22.5	41.0
38-39	35.40636794764151	40.0	36.5	41.0	21.5	41.0
40-41	35.282581028788165	40.0	36.0	41.0	19.5	41.0
42-43	35.14725758904095	40.0	36.0	41.0	17.5	41.0
44-45	35.00303667583982	40.0	35.0	41.0	15.0	41.0
46-47	34.81033820596423	40.0	35.0	41.0	12.0	41.0
48-49	34.61397367507966	40.0	35.0	41.0	8.5	41.0
50-51	34.427922651509334	40.0	35.0	41.0	7.5	41.0
52-53	34.23251130310872	39.0	35.0	41.0	4.5	41.0
54-55	34.021336852504696	39.0	35.0	41.0	2.0	41.0
56-57	33.770553845705614	39.0	35.0	41.0	2.0	41.0
58-59	33.7014032541299	38.5	35.0	41.0	2.0	41.0
60-61	33.45694257199689	37.5	34.5	41.0	2.0	41.0
62-63	33.16631792790908	37.0	34.0	40.0	2.0	41.0
64-65	32.859034607245114	36.5	34.0	40.0	2.0	41.0
66-67	32.535168758657775	36.0	34.0	39.0	2.0	41.0
68-69	32.19867944992862	35.5	34.0	39.0	2.0	41.0
70-71	31.869409364562667	35.0	33.5	38.5	2.0	40.5
72-73	31.532583346355857	35.0	33.0	37.0	2.0	39.5
74-75	31.202516679865766	35.0	33.0	37.0	2.0	39.0
76-77	30.9097393464724	35.0	33.0	36.0	2.0	39.0
78-79	30.6577965625836	35.0	33.0	36.0	2.0	37.0
80-81	30.43626589520356	35.0	33.0	35.5	2.0	37.0
82-83	30.244019351028072	35.0	33.0	35.0	2.0	36.5
84-85	30.083724119390492	35.0	32.0	35.0	2.0	36.0
86-87	29.94731307823314	35.0	32.0	35.0	2.0	36.0
88-89	29.82927372725804	35.0	32.0	35.0	2.0	36.0
90-91	29.732452701754198	35.0	32.0	35.0	2.0	35.0
92-93	29.66932348212559	35.0	32.0	35.0	2.0	35.0
94-95	29.60354428938077	35.0	32.0	35.0	2.0	35.0
96-97	29.54427580644734	35.0	32.0	35.0	2.0	35.0
98-99	29.48299302610773	35.0	32.0	35.0	2.0	35.0
100-101	29.43133576655869	35.0	32.0	35.0	2.0	35.0
102-103	28.754352392127156	34.0	30.0	35.0	2.0	35.0
104-105	29.3065589152194	35.0	31.5	35.0	2.0	35.0
106-107	29.36896207399795	35.0	32.0	35.0	2.0	35.0
108-109	29.439347598072843	35.0	32.0	35.0	2.0	35.0
110-111	29.55133975277512	35.0	32.0	35.0	2.0	35.0
112-113	29.514046446705215	35.0	32.0	35.0	2.0	35.0
114-115	29.456611821979017	35.0	32.0	35.0	2.0	35.0
116-117	29.398153825575882	35.0	32.0	35.0	2.0	35.0
118-119	29.322812679611495	35.0	32.0	35.0	2.0	35.0
120-121	29.230540708407606	35.0	32.0	35.0	2.0	35.0
122-123	29.11922495239054	35.0	32.0	35.0	2.0	35.0
124-125	28.586717092525248	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	97749.0
3	6832.0
4	4967.0
5	4493.0
6	4603.0
7	4942.0
8	5010.0
9	5081.0
10	5077.0
11	5317.0
12	5414.0
13	6297.0
14	7138.0
15	6316.0
16	4503.0
17	3898.0
18	3540.0
19	3293.0
20	3156.0
21	3354.0
22	3845.0
23	4889.0
24	6235.0
25	8209.0
26	9599.0
27	11447.0
28	13535.0
29	16077.0
30	19388.0
31	24155.0
32	31395.0
33	41899.0
34	63614.0
35	110405.0
36	254916.0
37	525777.0
38	173668.0
39	171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.69312322592305	17.278446360418016	11.284391843764286	27.74403856989465
2	16.27515223108931	18.94836724045228	36.813966854809024	27.962513673649386
3	19.416648346845857	22.126348493316133	28.109844762694316	30.347158397143698
4	13.684917417631215	14.993506394595308	30.824989221475985	40.49658696629749
5	14.647607044542468	36.83300719710789	32.05923518291267	16.460150575436966
6	35.667434041636994	32.67155799201709	15.954288742774805	15.706719223571108
7	31.08930459040802	30.876898540511473	18.268178699256346	19.76561816982417
8	28.68712795623517	34.19124001454236	17.484740783711494	19.636891245510974
9	27.75891228827645	15.254425848251712	17.699419775830304	39.28724208764153
10-11	27.28287964835759	24.577589086795165	25.218476986538935	22.92105427830831
12-13	26.736219808599664	21.919409652966394	26.060332613486803	25.28403792494714
14-15	24.243910094265413	23.44365396992724	23.206997200378225	29.105438735429125
16-17	24.69278984825891	26.487414945265677	23.941566834679286	24.87822837179613
18-19	24.706033092218004	25.577835842045182	25.43977502377162	24.27635604196519
20-21	25.384782453231487	24.428918212373958	25.55442840834748	24.63187092604708
22-23	25.27754528527272	24.078204004227246	25.329624342141855	25.314626368358184
24-25	24.846808775503177	24.605715519227868	25.234405418075966	25.31307028719299
26-27	24.609092546437434	24.86137634385818	25.357037857137183	25.1724932525672
28-29	24.677361469046566	25.061581084409788	25.355117586763114	24.90593985978053
30-31	25.054429732671878	24.724441201321145	25.289000691297336	24.93212837470964
32-33	24.823897963453945	24.788670934522752	25.276154744657013	25.11127635736629
34-35	24.89445134564602	24.889948642699927	25.419115563195437	24.79648444845862
36-37	24.93477702350146	24.866706749551717	25.453316240719797	24.745199986227025
38-39	24.80774120582385	25.13173716928309	25.473810160746496	24.586711464146564
40-41	24.79370336722721	25.03555811003017	25.37902164210928	24.791716880633345
42-43	24.67878511777217	24.98741891823886	25.202058794705877	25.13173716928309
44-45	24.666402684670416	25.06035608434357	25.289695961605187	24.983545269380826
46-47	24.589757410257157	25.060091219464393	25.100152032440647	25.249999337837803
48-49	24.584393896453722	25.281948663889114	25.044828380801533	25.088829058855627
50-51	24.482056728759822	25.40600475167593	25.118957438862566	24.99298108070168
52-53	24.738479039917785	25.147397305264718	25.088133788547772	25.02598986626972
54-55	24.538042542596894	24.98069797192962	25.223314201260226	25.257945284213257
56-57	24.46457564673382	25.254237175904713	24.97208986335621	25.309097314005257
58-59	24.51867429830672	25.29184798874854	25.172062847138534	25.01741486580621
60-61	24.755629040844813	25.077704733929984	25.376273668987764	24.790392556237435
62-63	24.638922953455296	25.190868253560446	25.209607443762565	24.960601349221694
64-65	24.532116190925198	24.97427499860946	25.119387844291236	25.374220966174104
66-67	24.505894567886195	25.179313523206137	25.094689194307524	25.220102714600145
68-69	24.292380367155694	25.191166226549527	25.24635744574905	25.27009596054573
70-71	24.21742360634722	25.07105000384054	25.3699169118874	25.341609477924838
72-73	24.375779695988093	25.215732444093646	25.119950682159498	25.28853717775877
74-75	24.23265333690018	25.312540557434627	25.114256087257086	25.34055001840811
76-77	24.338301315583855	24.990497972459348	25.092636491493863	25.57856422046293
78-79	24.174283739150475	25.10558176246388	25.285921637076846	25.4342128613088
80-81	24.302114151465627	25.31396420616023	25.05396621913331	25.329955423240836
82-83	24.31817158476603	25.181631090898975	25.202588524464243	25.297608799870748
84-85	24.27814387989967	25.28423312347206	24.99357702667984	25.44404596994843
86-87	24.042381029317895	25.393655426684074	25.107667573387438	25.456295970610594
88-89	23.99344724288904	25.16097163032279	25.179081766436852	25.66649936035132
90-91	24.119364204048267	25.11502604122695	25.24921343277702	25.516396321947767
92-93	24.057218930346618	25.166310808605086	25.300266620403463	25.47620364064483
94-95	24.170599638128	25.056110024980093	25.128318884388023	25.644971452503885
96-97	23.960203946497153	24.983810091378626	25.220599920540327	25.835386041583895
98-99	23.868207165458166	25.136049545640702	25.075263035743195	25.920480253157933
100-101	23.906604670627278	25.215964200862928	25.21546757921446	25.661963549295326
102-103	23.653294521799705	25.063534462893756	25.35402501913649	25.929145996170057
104-105	23.78857425884185	25.208018254487474	25.111441897915775	25.891965588754896
106-107	23.8066244802432	24.993783942076604	25.250041136894648	25.94955044078555
108-109	23.745380575672478	25.013756465207564	25.20128142052802	26.039581538591932
110-111	23.593423522240922	25.336072925416193	25.23049091825887	25.84001263408402
112-113	23.55275312128566	25.31845225285476	25.255712010700606	25.87308261515897
114-115	23.527174040797895	25.305547177344994	25.242542298191534	25.924736483665576
116-117	23.31331607730647	25.293355767492585	25.250877865602966	26.142450289597978
118-119	23.425855233839734	25.158273633774574	25.268093452460967	26.147777679924726
120-121	23.344434500958975	25.160119361555395	25.359132539598196	26.136313597887433
122-123	23.234897009034018	25.346630635665523	25.162116329102275	26.256356026198187
124-125	23.058894419788075	25.21330748690987	25.18049731741273	26.547300775889326
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	47.0
1	39.0
2	35.5
3	40.5
4	39.5
5	49.0
6	119.0
7	278.0
8	389.0
9	445.0
10	443.0
11	378.5
12	402.0
13	446.5
14	467.5
15	510.0
16	554.0
17	598.5
18	654.0
19	713.0
20	777.0
21	835.0
22	909.5
23	1015.5
24	1115.5
25	1311.5
26	1616.0
27	1960.0
28	2482.0
29	3324.5
30	4432.5
31	5665.5
32	7377.0
33	9327.5
34	11406.5
35	13753.0
36	16526.5
37	19810.5
38	22920.5
39	26359.5
40	30099.0
41	33431.0
42	37127.5
43	41492.0
44	45811.5
45	50273.5
46	54751.0
47	58830.5
48	63633.5
49	67555.0
50	69271.0
51	71262.5
52	72552.5
53	71832.5
54	70837.5
55	68683.5
56	64563.5
57	59438.5
58	54877.0
59	50154.5
60	43726.0
61	37085.0
62	30330.5
63	23471.5
64	17820.5
65	13621.5
66	10432.5
67	8243.0
68	6653.5
69	5161.0
70	3971.0
71	3112.0
72	2385.0
73	1848.5
74	1414.0
75	1067.5
76	818.0
77	559.5
78	390.0
79	311.0
80	258.5
81	193.5
82	142.0
83	122.5
84	103.0
85	92.0
86	95.0
87	102.0
88	89.5
89	80.5
90	75.5
91	59.0
92	55.0
93	63.0
94	59.5
95	48.0
96	40.0
97	34.0
98	28.5
99	13.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.34405947805726905
2	0.0
3	0.0
4	0.017017568487436137
5	0.17573784733718095
6	0.04827162423089861
7	0.02383783912636968
8	0.008806757232797688
9	0.006489189539956191
10-11	0.00221824336314829
12-13	0.056581759815230254
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	1.6554054948867834E-4
92-93	2.97972989079621E-4
94-95	9.932432969320702E-5
96-97	2.6486487918188535E-4
98-99	3.310810989773567E-5
100-101	0.0
102-103	0.0
104-105	0.0
106-107	1.6554054948867834E-4
108-109	3.310810989773567E-4
110-111	2.3175676928414968E-4
112-113	5.95945978159242E-4
114-115	2.3175676928414968E-4
116-117	4.6351353856829937E-4
118-119	1.9864865938641404E-4
120-121	1.6554054948867834E-4
122-123	0.0015229730552958408
124-125	9.932432969320702E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1510204.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.66550698181285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.8021841457932	55.20049585280732
2	9.511914279241733	12.682331755863904
3	3.039010282270077	6.077914835714307
4	1.44202428973099	3.845331214200202
5	0.848776680394163	2.8292063856408505
6	0.5380444083275955	2.1521401955933213
7	0.3645669579853006	1.7012828758035135
8	0.26376013178337837	1.4066962325542953
9	0.2061106535476268	1.2366424091794792
>10	0.9444553564041523	10.551619373881103
>50	0.031894823917940136	1.42164675035502
>100	0.006860343737724356	0.6481553500702059
>500	1.988234330527894E-4	0.09311402137440936
>1k	1.988234330527894E-4	0.1534227469621051
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	6.621621979547134E-5	0.0	0.0	0.0	1.9864865938641401E-4
7	6.621621979547134E-5	0.0	0.0	0.0	1.9864865938641401E-4
8	6.621621979547134E-5	0.0	0.0	0.0	1.9864865938641401E-4
9	6.621621979547134E-5	0.0	0.0	0.0	1.9864865938641401E-4
10-11	6.621621979547134E-5	0.0	0.0	0.0	1.9864865938641401E-4
12-13	6.621621979547134E-5	0.0	0.0	0.0	2.6486487918188535E-4
14-15	6.621621979547134E-5	0.0	0.0	0.0	2.97972989079621E-4
16-17	6.621621979547134E-5	0.0	0.0	0.0	3.310810989773567E-4
18-19	6.621621979547134E-5	0.0	0.0	0.0	3.310810989773567E-4
20-21	6.621621979547134E-5	0.0	0.0	9.932432969320701E-5	3.310810989773567E-4
22-23	6.621621979547134E-5	0.0	0.0	2.3175676928414968E-4	3.310810989773567E-4
24-25	6.621621979547134E-5	0.0	0.0	6.95270307852449E-4	3.310810989773567E-4
26-27	6.621621979547134E-5	0.0	0.0	0.001092567626625277	4.6351353856829937E-4
28-29	6.621621979547134E-5	0.0	0.0	0.0030790542204894174	4.6351353856829937E-4
30-31	9.932432969320701E-5	0.0	0.0	0.012547973651241818	4.6351353856829937E-4
32-33	1.3243243959094268E-4	0.0	0.0	0.028903379940723242	5.297297583637707E-4
34-35	1.6554054948867834E-4	0.0	0.0	0.04913243508823974	5.297297583637707E-4
36-37	2.6486487918188535E-4	0.0	0.0	0.07803581502896298	5.297297583637707E-4
38-39	2.6486487918188535E-4	0.0	0.0	0.1233608174789631	5.95945978159242E-4
40-41	2.6486487918188535E-4	0.0	0.0	0.1788500096675681	5.95945978159242E-4
42-43	2.6486487918188535E-4	0.0	0.0	0.22811487719539877	5.95945978159242E-4
44-45	2.6486487918188535E-4	3.310810989773567E-5	0.0	0.287080420923266	5.95945978159242E-4
46-47	2.6486487918188535E-4	6.621621979547134E-5	0.0	0.3496878567398841	6.290540880569777E-4
48-49	2.6486487918188535E-4	6.621621979547134E-5	0.0	0.4175594820302423	6.621621979547134E-4
50-51	2.6486487918188535E-4	6.621621979547134E-5	0.0	0.48258380986939514	6.621621979547134E-4
52-53	2.6486487918188535E-4	6.621621979547134E-5	0.0	0.5535013812703449	6.95270307852449E-4
54-55	2.6486487918188535E-4	9.932432969320701E-5	0.0	0.634152736981229	7.283784177501847E-4
56-57	2.6486487918188535E-4	1.9864865938641401E-4	0.0	0.7226838228477741	7.283784177501847E-4
58-59	2.6486487918188535E-4	3.310810989773567E-4	0.0	0.8167439630672413	7.283784177501847E-4
60-61	3.310810989773567E-4	3.310810989773567E-4	0.0	0.9105392384075264	7.283784177501847E-4
62-63	3.310810989773567E-4	3.310810989773567E-4	0.0	1.0159885684318146	7.283784177501847E-4
64-65	3.310810989773567E-4	3.310810989773567E-4	0.0	1.1235568174895576	7.945946375456561E-4
66-67	3.310810989773567E-4	3.310810989773567E-4	0.0	1.2477784458258618	7.945946375456561E-4
68-69	3.9729731877282803E-4	3.310810989773567E-4	0.0	1.3825946693294417	7.945946375456561E-4
70-71	3.9729731877282803E-4	3.310810989773567E-4	0.0	1.5125440006780542	8.608108573411274E-4
72-73	3.9729731877282803E-4	3.310810989773567E-4	0.0	1.6322298179583685	0.0010594595167275414
74-75	3.9729731877282803E-4	3.310810989773567E-4	0.0	1.7688007712865281	0.001092567626625277
76-77	3.9729731877282803E-4	3.310810989773567E-4	0.0	1.9345068613246954	0.0011256757365230127
78-79	3.9729731877282803E-4	3.310810989773567E-4	0.0	2.120011601081708	0.0011256757365230127
80-81	3.9729731877282803E-4	3.310810989773567E-4	0.0	2.3009143135629357	0.0011256757365230127
82-83	3.9729731877282803E-4	3.310810989773567E-4	0.0	2.480691350307641	0.001191891956318484
84-85	3.9729731877282803E-4	3.310810989773567E-4	0.0	2.6772210906605993	0.001191891956318484
86-87	3.9729731877282803E-4	3.310810989773567E-4	0.0	2.9017934000969405	0.001191891956318484
88-89	3.9729731877282803E-4	3.310810989773567E-4	0.0	3.1492765215825145	0.001191891956318484
90-91	3.9729731877282803E-4	3.310810989773567E-4	0.0	3.407254913905671	0.001191891956318484
92-93	3.9729731877282803E-4	3.310810989773567E-4	0.0	3.6551353327100182	0.001191891956318484
94-95	3.9729731877282803E-4	3.310810989773567E-4	0.0	3.913378589912356	0.0012250000662162198
96-97	3.9729731877282803E-4	3.310810989773567E-4	0.0	4.211781984420648	0.0013243243959094268
98-99	3.9729731877282803E-4	3.310810989773567E-4	0.0	4.540115110276492	0.001390540615704898
100-101	3.9729731877282803E-4	3.310810989773567E-4	0.0	4.888048237191796	0.001390540615704898
102-103	3.9729731877282803E-4	3.9729731877282803E-4	0.0	5.248363797208854	0.001390540615704898
104-105	3.9729731877282803E-4	3.9729731877282803E-4	0.0	5.61235435742456	0.001390540615704898
106-107	3.9729731877282803E-4	3.9729731877282803E-4	0.0	6.0050827570315	0.001390540615704898
108-109	3.9729731877282803E-4	3.9729731877282803E-4	0.0	6.439825348098667	0.0014236487256026338
110-111	3.9729731877282803E-4	3.9729731877282803E-4	0.0	6.901948346051262	0.0015229730552958408
112-113	3.9729731877282803E-4	3.9729731877282803E-4	0.0	7.34764972149458	0.0015891892750913121
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1750	0.0	61.463127	1
GTATCAA	2645	0.0	47.217266	1
GTACATG	7010	0.0	39.55307	1
TACATGG	7205	0.0	38.299026	2
ACATGGG	7315	0.0	37.56015	3
CATGGGG	6700	0.0	35.047913	4
ATGGGGG	4015	0.0	33.102444	5
TCAACGC	3530	0.0	32.92309	4
TATCAAC	3850	0.0	32.663483	2
CAACGCA	3550	0.0	32.40184	5
ATCAACG	3750	0.0	31.62733	3
AACGCAG	3710	0.0	30.990065	6
TGGGGGG	2770	0.0	24.301762	6
ACGCAGA	4775	0.0	23.820734	7
GTGGTAT	1105	0.0	22.712622	1
TACGTAA	190	3.2642587E-5	21.957615	5
GAGTACA	4910	0.0	21.663006	1
TTACGTA	195	3.9884733E-5	21.394598	4
CGCAGAG	5355	0.0	21.237896	8
AGTACAT	4880	0.0	20.762032	2
>>END_MODULE