FastQCFastQC Report
Mon 15 Aug 2016
SRR2921285_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921285_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1659616
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14000.050.5846751
GTATCAA23350.049.1892781
GTACATG84150.039.603531
TACATGG85750.038.5858082
TCAACGC28950.038.2341354
ACATGGG87050.037.3943063
CAACGCA30000.036.697585
TATCAAC32600.034.5009572
ATCAACG32550.034.18833
CATGGGG82000.034.1817254
ATGGGGG49600.034.0740365
AACGCAG32550.033.8195956
TGGGGGG34700.028.9804346
ACGCAGA42600.025.4173877
CGCAGAG45400.023.9808398
GAGTACA57300.022.8490471
AGTACAT57200.022.2640742
AGAGTAC46200.022.0262745
CTAGTAC2755.509537E-821.6398493
GTATATG4600.020.699611
Produced by FastQC (version 0.11.5)