##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921285_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1659616
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.51494984381929	31.0	31.0	34.0	27.0	34.0
2	30.712310558587046	33.0	31.0	34.0	27.0	34.0
3	30.797341071669592	33.0	31.0	34.0	27.0	34.0
4	34.27291011896728	37.0	35.0	37.0	31.0	37.0
5	34.208752506603936	37.0	35.0	37.0	31.0	37.0
6	34.34557090314868	37.0	35.0	37.0	31.0	37.0
7	34.267870398935656	37.0	35.0	37.0	31.0	37.0
8	34.29449101478896	37.0	35.0	37.0	31.0	37.0
9	35.92966264485278	39.0	37.0	39.0	31.0	39.0
10-11	35.92242542853286	39.0	37.0	39.0	31.0	39.0
12-13	35.85098601122188	39.0	37.0	39.0	31.0	39.0
14-15	37.18686551587837	41.0	38.0	41.0	31.0	41.0
16-17	37.16830218556582	41.0	38.0	41.0	31.0	41.0
18-19	37.157409304321	41.0	38.0	41.0	31.0	41.0
20-21	37.18071559927115	41.0	38.0	41.0	31.0	41.0
22-23	37.124088644602125	41.0	38.0	41.0	31.0	41.0
24-25	37.09987069297958	41.0	38.0	41.0	30.5	41.0
26-27	37.06030943302547	41.0	38.0	41.0	30.0	41.0
28-29	36.99279321240576	40.0	38.0	41.0	30.0	41.0
30-31	36.915942904864735	40.0	38.0	41.0	30.0	41.0
32-33	36.836070211422395	40.0	38.0	41.0	30.0	41.0
34-35	36.7293048512427	40.0	38.0	41.0	30.0	41.0
36-37	36.624382989800054	40.0	38.0	41.0	30.0	41.0
38-39	36.5270198045813	40.0	37.0	41.0	30.0	41.0
40-41	36.424690711586294	40.0	37.0	41.0	29.0	41.0
42-43	36.232731848813216	40.0	37.0	41.0	28.5	41.0
44-45	35.98640589148333	40.0	36.0	41.0	28.0	41.0
46-47	35.77132601758479	40.0	36.0	41.0	27.0	41.0
48-49	35.57182956780363	40.0	35.0	41.0	27.0	41.0
50-51	35.394528915122535	39.0	35.0	41.0	26.0	41.0
52-53	35.19725647378671	39.0	35.0	41.0	26.0	41.0
54-55	34.98664450089659	39.0	35.0	41.0	26.0	41.0
56-57	34.73646915913079	38.5	35.0	40.5	25.5	41.0
58-59	34.457384117771824	38.0	34.0	40.0	24.5	41.0
60-61	34.166918431733606	37.5	34.0	40.0	24.0	41.0
62-63	33.837424741626975	37.0	34.0	40.0	23.0	41.0
64-65	33.4844548377456	36.5	34.0	39.5	22.5	41.0
66-67	33.139805533328186	36.0	33.0	39.0	22.0	41.0
68-69	32.7844679130594	35.5	33.0	39.0	21.5	40.0
70-71	32.722944343751806	35.0	33.0	38.5	22.0	40.0
72-73	32.4342616002738	35.0	33.0	37.0	21.5	39.5
74-75	32.11513506738908	35.0	33.0	37.0	20.5	39.0
76-77	31.816701574340087	35.0	33.0	36.5	20.0	39.0
78-79	31.53604568767715	35.0	33.0	36.0	20.0	37.5
80-81	31.274176375739927	35.0	33.0	35.5	18.5	37.0
82-83	31.067150774637025	35.0	33.0	35.0	18.0	37.0
84-85	30.859274675587606	35.0	32.0	35.0	17.5	36.0
86-87	30.6966792318223	35.0	32.0	35.0	15.5	36.0
88-89	30.568132628270636	35.0	32.0	35.0	13.5	36.0
90-91	30.454177050594836	35.0	32.0	35.0	10.5	35.0
92-93	30.350945640437306	35.0	32.0	35.0	8.5	35.0
94-95	30.234883250101227	35.0	32.0	35.0	7.0	35.0
96-97	30.146805947881923	35.0	32.0	35.0	7.0	35.0
98-99	30.063960578832692	35.0	32.0	35.0	3.5	35.0
100-101	29.976233960144995	35.0	31.5	35.0	2.0	35.0
102-103	29.13773216213871	33.5	29.5	34.5	2.0	35.0
104-105	29.743044776623027	34.0	31.0	35.0	2.0	35.0
106-107	29.825434317336057	35.0	31.0	35.0	2.0	35.0
108-109	29.76580064304032	35.0	31.0	35.0	2.0	35.0
110-111	29.67974850808862	35.0	31.0	35.0	2.0	35.0
112-113	29.593342074311167	34.5	31.0	35.0	2.0	35.0
114-115	29.471112293446197	34.0	31.0	35.0	2.0	35.0
116-117	29.34087795008002	34.0	31.0	35.0	2.0	35.0
118-119	29.336988194859536	34.5	31.0	35.0	2.0	35.0
120-121	29.50272201521316	35.0	32.0	35.0	2.0	35.0
122-123	29.369659005456683	35.0	32.0	35.0	2.0	35.0
124-125	28.80220424483736	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	55626.0
3	8082.0
4	5067.0
5	4297.0
6	4542.0
7	5066.0
8	5270.0
9	5582.0
10	6029.0
11	6506.0
12	6608.0
13	6491.0
14	6370.0
15	5639.0
16	4604.0
17	4283.0
18	4181.0
19	4294.0
20	4283.0
21	4381.0
22	5072.0
23	6462.0
24	8965.0
25	11379.0
26	12858.0
27	14505.0
28	17712.0
29	21257.0
30	25783.0
31	32478.0
32	41897.0
33	57889.0
34	89824.0
35	149834.0
36	319478.0
37	540916.0
38	146055.0
39	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.436613759674856	17.10199376012082	12.13847541804599	25.322917062158336
2	16.531717641160135	18.699981680037027	39.10848069654428	25.659819982258565
3	20.492204373650107	22.922552356525763	27.864784210120337	28.720459059703796
4	13.841306726963296	14.372056079722686	34.13971236964791	37.646924823666104
5	14.922157709354632	37.06932136949402	32.54232589811654	15.466195023034809
6	35.2325481870994	32.917614495971804	16.79300372380887	15.056833593119933
7	30.930646981862186	31.013570968408732	19.36238927146818	18.693392778260897
8	27.929030597886058	35.02018545670838	18.39959068630553	18.651193259100026
9	27.906612375553575	15.902748442404677	18.02622436724479	38.16441481479696
10-11	27.086612512504065	24.939588781892905	26.106263483301795	21.86753522230123
12-13	26.71281744730905	22.173706589020227	26.520438834146436	24.593037129524287
14-15	24.254676940051468	23.670373911154485	23.466245977323872	28.608703171470175
16-17	24.83805616784552	26.85477660488597	24.171570677410298	24.135596549858214
18-19	24.789635178831187	26.2059671057151	25.468515177481382	23.535882537972327
20-21	25.550982924794436	25.161892520780967	25.19946381811756	24.08766073630704
22-23	25.398054005065347	24.74973049289821	25.14457572140227	24.707639780634175
24-25	24.917852208092874	25.06190083934465	25.106492342012004	24.91375461055047
26-27	24.653019019251847	25.243999954203872	25.23776324487249	24.865217781671788
28-29	24.689470281162492	25.698567583186048	25.088897097504493	24.523065038146964
30-31	25.305217830668848	25.238812747004165	25.1563185913445	24.299650830982493
32-33	24.94313677695425	25.184449561891803	25.097945752982366	24.774467908171584
34-35	24.934851050021198	25.40223017457719	25.221599833440443	24.441318941961164
36-37	24.89215014555738	25.43906746860317	25.19983633456926	24.468946051270184
38-39	24.931499419997287	25.543018123201616	25.141806896759515	24.383675560041578
40-41	24.917149827520387	25.29371549918152	25.271509634241983	24.51762503905611
42-43	24.755729277927642	25.313373673267026	25.10978586370855	24.821111185096782
44-45	24.63636941583189	25.265602859209768	25.362078892809464	24.73594883214888
46-47	24.675235848345974	25.211804017632506	25.25474031824085	24.85821981578067
48-49	24.56203994685002	25.423979559554187	25.26561431313293	24.748366180462863
50-51	24.573028186391557	25.44208172730869	25.367692142212157	24.617197944087597
52-53	24.732730405627915	25.390001346780245	25.24890577871165	24.628362468880187
54-55	24.520962206353307	25.083391157027258	25.417287210563916	24.978359426055523
56-57	24.58437206421059	25.2963433660123	25.18534453927352	24.93394003050359
58-59	24.623835365245547	25.489679143369955	25.280366524563874	24.60611896682062
60-61	24.979007452467357	25.290573916816182	25.356707597238355	24.373711033478106
62-63	24.777494561647714	25.32581906707402	25.390567095106388	24.50611927617188
64-65	24.70599613327633	25.098423091396466	25.29073489743722	24.904845877889986
66-67	24.619721314256154	25.394815851023733	25.19924955085432	24.786213283865795
68-69	24.538564720855007	25.335644075068576	25.340856501009945	24.784934703066472
70-71	24.486909059246152	25.17725699303807	25.555497640741283	24.780336306974494
72-73	24.581275905215648	25.52422048217709	25.162763917975845	24.73173969463142
74-75	24.51426298853899	25.54918832929607	25.248231437627293	24.688317244537643
76-77	24.49027675339012	25.179810162889687	25.32147695007255	25.00843613364764
78-79	24.42838454497632	25.270750944080657	25.372404945675065	24.928459565267953
80-81	24.579119101204842	25.447243387534613	25.172651704824485	24.800985806436064
82-83	24.679318957647265	25.48982684700048	25.23671072572408	24.594143469628175
84-85	24.705298880251746	25.56613378465147	24.995126354715204	24.733440980381584
86-87	24.33010682255476	25.71120127316914	25.2144495779102	24.744242326365896
88-89	24.365805547546245	25.38802483720507	25.228159960085	25.018009655163688
90-91	24.602624623835652	25.191351202822034	25.324462463106666	24.881561710235648
92-93	24.5632250644711	25.492380222656163	25.294454928080718	24.64993978479202
94-95	24.596267939671662	25.36445783949302	25.257948924531128	24.781325296304185
96-97	24.401560525502262	25.323467360394254	25.306835253558273	24.968136860545208
98-99	24.340880248462476	25.350319315739934	25.182827694840448	25.125972740957142
100-101	24.283517358949684	25.39336567716867	25.34452451729809	24.97859244658356
102-103	24.095164034519755	25.429079064436657	25.455864022799453	25.01989287824414
104-105	24.31988801611546	25.431742646764178	25.361933493600127	24.88643584352024
106-107	24.329804715817435	25.368202911877763	25.40077337525814	24.901218997046666
108-109	24.3236313390825	25.3227051492419	25.405351171777784	24.948312339897814
110-111	24.158338457959932	25.62085315767891	25.31690078250864	24.903907601852517
112-113	24.12315627885816	25.752274380957115	25.350500973927666	24.774068366257058
114-115	24.22533233829437	25.743861424052604	25.28994770622328	24.740858531429744
116-117	24.12301117798641	25.57957502501421	25.365572066995007	24.931841730004372
118-119	24.256088496520622	25.457295497706923	25.397844744206346	24.888771261566113
120-121	24.057301054410267	25.557170586430967	25.537766110987576	24.847762248171186
122-123	24.024777502606096	25.76950655892791	25.40389980537127	24.801816133094718
124-125	24.05519826992476	25.683982884836592	25.35103740040408	24.909781444834568
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	266.0
1	224.0
2	175.5
3	151.0
4	124.5
5	103.5
6	92.0
7	119.0
8	163.5
9	178.5
10	193.5
11	216.0
12	229.5
13	245.5
14	276.0
15	311.0
16	332.0
17	356.5
18	384.0
19	421.5
20	464.5
21	507.5
22	574.0
23	726.0
24	899.0
25	1123.5
26	1444.5
27	1939.5
28	2603.5
29	3619.0
30	5306.0
31	7415.0
32	10047.5
33	12674.5
34	15505.5
35	18503.5
36	21826.5
37	25357.0
38	28394.0
39	32077.0
40	35936.0
41	38858.0
42	42367.5
43	47713.5
44	52837.0
45	58004.5
46	62001.0
47	65431.0
48	70754.0
49	74670.5
50	76144.0
51	76821.0
52	76843.0
53	76388.5
54	76040.5
55	73876.5
56	69396.5
57	63709.5
58	58149.0
59	52639.5
60	45453.5
61	38254.0
62	31520.5
63	24244.0
64	17822.5
65	13516.0
66	10437.0
67	8071.0
68	6340.0
69	4893.5
70	3798.5
71	2874.0
72	2148.5
73	1683.0
74	1313.0
75	997.0
76	742.5
77	491.5
78	310.5
79	186.5
80	121.0
81	92.0
82	64.5
83	46.0
84	31.0
85	24.5
86	26.0
87	19.5
88	11.0
89	9.0
90	10.5
91	8.0
92	6.0
93	4.0
94	3.5
95	4.0
96	3.5
97	2.5
98	3.0
99	3.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01741366677592889
2	0.013497098123903362
3	0.013497098123903362
4	0.015907294217457534
5	0.010303588299944084
6	0.03386325511443611
7	0.015967549119796386
8	0.015244490291730134
9	0.010062568690588667
10-11	0.00939976476486127
12-13	0.00771262749937335
14-15	0.007079951024815378
16-17	0.005422941210496886
18-19	0.007110078475984807
20-21	0.006296637294410273
22-23	0.0063267647455797
24-25	0.006447274550257409
26-27	0.005242176503480324
28-29	0.006808803964290535
30-31	0.006296637294410273
32-33	0.009128617704336425
34-35	0.009008107899658717
36-37	0.00801390201106762
38-39	0.008495941229778455
40-41	0.008706833387964444
42-43	0.008164539266914756
44-45	0.008224794169253612
46-47	0.010906137323332626
48-49	0.00970103927655554
50-51	0.006597911806104545
52-53	0.006176127489732564
54-55	0.007079951024815378
56-57	0.008375431425100745
58-59	0.008224794169253612
60-61	0.005935107880377147
62-63	0.0063870196479185555
64-65	0.004308225517228082
66-67	0.004609500028922353
68-69	0.0072305882806625145
70-71	0.005453068661666313
72-73	0.00485051963827777
74-75	0.004579372577752926
76-77	0.0053024314058191776
78-79	0.003916568652025529
80-81	0.0036755490426701115
82-83	0.006085745136224284
84-85	0.0108760098721632
86-87	0.008134411815745328
88-89	0.005392813759327458
90-91	0.0061460000385631375
92-93	0.009128617704336425
94-95	0.007863264755220485
96-97	0.010544607909299501
98-99	0.007833137304051057
100-101	0.009339509862522416
102-103	0.006176127489732564
104-105	0.005513323564005168
106-107	0.008254921620423038
108-109	0.0077728824017122035
110-111	0.008767088290303298
112-113	0.005935107880377147
114-115	0.0029826176657732875
116-117	0.0035851666891618303
118-119	0.16765926575786205
120-121	0.012352254979465129
122-123	0.0027717255075872973
124-125	0.0021089215818599003
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1659616.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.29712668729979
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.54387433763341	51.614929445224355
2	10.107974514961171	12.796114868509894
3	3.2434873244582425	6.159102842546532
4	1.5613288212522571	3.9531051279734624
5	0.8769874053797094	2.7755391450742897
6	0.5951911457686883	2.2604333614127863
7	0.40774688790947594	1.8066444494250828
8	0.29112153946274355	1.4741725571820017
9	0.21827379758349988	1.2434493796344739
>10	1.0866113983358299	11.901259375316428
>50	0.05160309820049044	2.2163853827107025
>100	0.01541908413363758	1.6267745999921253
>500	3.806449209805863E-4	0.17208946499782912
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	6.025490233885429E-5	0.0
4	0.0	0.0	0.0	6.025490233885429E-5	0.0
5	0.0	0.0	0.0	6.025490233885429E-5	0.0
6	0.0	0.0	0.0	6.025490233885429E-5	0.0
7	0.0	0.0	0.0	6.025490233885429E-5	0.0
8	0.0	0.0	0.0	6.025490233885429E-5	0.0
9	0.0	0.0	0.0	6.025490233885429E-5	6.025490233885429E-5
10-11	0.0	0.0	0.0	6.025490233885429E-5	6.025490233885429E-5
12-13	0.0	0.0	0.0	6.025490233885429E-5	6.025490233885429E-5
14-15	0.0	0.0	0.0	6.025490233885429E-5	6.025490233885429E-5
16-17	0.0	0.0	0.0	6.025490233885429E-5	6.025490233885429E-5
18-19	0.0	6.025490233885429E-5	0.0	6.025490233885429E-5	6.025490233885429E-5
20-21	0.0	6.025490233885429E-5	0.0	6.025490233885429E-5	9.038235350828143E-5
22-23	0.0	6.025490233885429E-5	0.0	1.8076470701656287E-4	2.1089215818599E-4
24-25	0.0	6.025490233885429E-5	0.0	4.5191176754140716E-4	3.6152941403312574E-4
26-27	0.0	6.025490233885429E-5	0.0	8.736960839133873E-4	3.6152941403312574E-4
28-29	0.0	6.025490233885429E-5	0.0	0.002500578447062453	3.916568652025529E-4
30-31	0.0	6.025490233885429E-5	0.0	0.008736960839133872	4.5191176754140716E-4
32-33	0.0	1.2050980467770857E-4	0.0	0.02145074523263213	6.025490233885429E-4
34-35	0.0	1.2050980467770857E-4	0.0	0.0371772747430731	6.025490233885429E-4
36-37	0.0	1.5063725584713573E-4	0.0	0.060164519985346006	6.628039257273972E-4
38-39	0.0	1.8076470701656287E-4	0.0	0.09969173591963443	6.628039257273972E-4
40-41	0.0	1.8076470701656287E-4	0.0	0.1479559126930567	6.628039257273972E-4
42-43	0.0	1.8076470701656287E-4	0.0	0.19209262865626747	6.628039257273972E-4
44-45	0.0	2.1089215818599E-4	0.0	0.23936259954109868	6.628039257273972E-4
46-47	0.0	2.4101960935541715E-4	0.0	0.2933509920367121	7.230588280662515E-4
48-49	0.0	2.4101960935541715E-4	0.0	0.3539975512407689	8.4356863274396E-4
50-51	0.0	2.4101960935541715E-4	0.0	0.4169940516360411	8.736960839133872E-4
52-53	0.0	2.4101960935541715E-4	0.0	0.4814667971386152	0.0010544607909299501
54-55	0.0	2.4101960935541715E-4	0.0	0.5566649152575054	0.0012050980467770858
56-57	0.0	2.4101960935541715E-4	0.0	0.6318931608275649	0.0012050980467770858
58-59	0.0	2.4101960935541715E-4	0.0	0.7183288182326514	0.0012954804002853672
60-61	0.0	2.711470605248443E-4	0.0	0.8103681815552514	0.0013256078514547944
62-63	0.0	3.0127451169427146E-4	0.0	0.9121688390567457	0.0013858627537936487
64-65	0.0	3.0127451169427146E-4	0.0	1.0132765651813431	0.0014159902049630758
66-67	0.0	3.0127451169427146E-4	0.0	1.1318582129842083	0.001446117656132503
68-69	0.0	4.2178431637198E-4	0.0	1.2606530667335094	0.001446117656132503
70-71	0.0	4.2178431637198E-4	0.0	1.3931234695254804	0.0015063725584713572
72-73	0.0	4.2178431637198E-4	0.0	1.5239368625031333	0.0015063725584713572
74-75	0.0	4.2178431637198E-4	0.0	1.6605045986541467	0.0015063725584713572
76-77	0.0	4.2178431637198E-4	0.0	1.8258139232207933	0.0015365000096407844
78-79	0.0	4.2178431637198E-4	0.0	2.004680600813682	0.0015666274608102115
80-81	0.0	4.2178431637198E-4	0.0	2.189301621579932	0.0015666274608102115
82-83	0.0	4.2178431637198E-4	0.0	2.3792853286543396	0.00168713726548792
84-85	0.0	4.2178431637198E-4	0.0	2.5801751730520794	0.00168713726548792
86-87	0.0	4.2178431637198E-4	0.0	2.8061611842739524	0.00168713726548792
88-89	0.0	4.2178431637198E-4	0.0	3.067305931010547	0.00168713726548792
90-91	0.0	4.2178431637198E-4	0.0	3.3383324817307134	0.00168713726548792
92-93	0.0	4.2178431637198E-4	0.0	3.596042699033993	0.0017473921678267744
94-95	0.0	4.2178431637198E-4	0.0	3.865713514451536	0.0017473921678267744
96-97	0.0	4.2178431637198E-4	0.0	4.176658937971194	0.0017473921678267744
98-99	0.0	4.2178431637198E-4	0.0	4.531259038235351	0.0017473921678267744
100-101	0.0	4.2178431637198E-4	0.0	4.900350442512003	0.0017473921678267744
102-103	0.0	4.2178431637198E-4	0.0	5.275678229180726	0.0018076470701656286
104-105	0.0	4.2178431637198E-4	0.0	5.660014123749108	0.001867901972504483
106-107	0.0	4.2178431637198E-4	0.0	6.071524979272313	0.00189802942367391
108-109	0.0	4.2178431637198E-4	0.0	6.521478462487708	0.0019281568748433372
110-111	0.0	4.2178431637198E-4	0.0	7.003246534138017	0.0019582843260127643
112-113	0.0	4.2178431637198E-4	0.0	7.486279958737443	0.0019884117771821917
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1400	0.0	50.584675	1
GTATCAA	2335	0.0	49.189278	1
GTACATG	8415	0.0	39.60353	1
TACATGG	8575	0.0	38.585808	2
TCAACGC	2895	0.0	38.234135	4
ACATGGG	8705	0.0	37.394306	3
CAACGCA	3000	0.0	36.69758	5
TATCAAC	3260	0.0	34.500957	2
ATCAACG	3255	0.0	34.1883	3
CATGGGG	8200	0.0	34.181725	4
ATGGGGG	4960	0.0	34.074036	5
AACGCAG	3255	0.0	33.819595	6
TGGGGGG	3470	0.0	28.980434	6
ACGCAGA	4260	0.0	25.417387	7
CGCAGAG	4540	0.0	23.980839	8
GAGTACA	5730	0.0	22.849047	1
AGTACAT	5720	0.0	22.264074	2
AGAGTAC	4620	0.0	22.026274	5
CTAGTAC	275	5.509537E-8	21.639849	3
GTATATG	460	0.0	20.69961	1
>>END_MODULE