FastQCFastQC Report
Mon 15 Aug 2016
SRR2921285_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921285_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1659616
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19350.063.0157131
GTATCAA31600.052.206151
GTACATG75600.039.848171
TACATGG79400.038.284982
TCAACGC41550.037.8714264
ACATGGG79550.037.388593
TATCAAC45350.036.5381242
CAACGCA43700.035.4626055
ATCAACG45000.035.232863
CATGGGG75000.035.1268964
AACGCAG45500.034.047366
ATGGGGG47050.032.6842735
CGTATAC1454.013147E-628.7745673
GTGGTAT9400.028.6143781
ACGCAGA54550.028.500297
CGCAGAG57900.026.4358088
TGGGGGG32400.024.4584036
GCAGAGT64450.023.1022959
GAGTACT26950.022.6371412-13
AGAGTAC59650.022.6122310-11
Produced by FastQC (version 0.11.5)