##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921285_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1659616
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.29465249792723	33.0	30.0	34.0	19.0	34.0
2	30.051401046989184	33.0	31.0	34.0	26.0	34.0
3	30.28351558432794	33.0	31.0	34.0	27.0	34.0
4	32.49429566839558	37.0	35.0	37.0	19.0	37.0
5	32.11726809093188	37.0	33.0	37.0	19.0	37.0
6	32.25234150550489	37.0	35.0	37.0	17.0	37.0
7	32.47516292925592	37.0	35.0	37.0	17.0	37.0
8	32.71444478722789	37.0	35.0	37.0	17.0	37.0
9	34.47455977768351	39.0	37.0	39.0	17.0	39.0
10-11	34.64974729093959	39.0	37.0	39.0	24.0	39.0
12-13	34.67712741983688	39.0	37.0	39.0	25.0	39.0
14-15	36.108063552050595	41.0	38.0	41.0	26.5	41.0
16-17	36.12609814559512	41.0	38.0	41.0	27.0	41.0
18-19	36.14675292356786	41.0	38.0	41.0	27.0	41.0
20-21	36.13475406359061	41.0	38.0	41.0	26.0	41.0
22-23	36.088877788596875	41.0	38.0	41.0	25.5	41.0
24-25	36.04080883770703	41.0	38.0	41.0	25.0	41.0
26-27	35.94523492181324	41.0	38.0	41.0	25.0	41.0
28-29	35.86723344436304	40.0	38.0	41.0	24.0	41.0
30-31	35.784213637371536	40.0	37.5	41.0	24.0	41.0
32-33	35.66725917320633	40.0	37.0	41.0	23.0	41.0
34-35	35.592759409405545	40.0	37.0	41.0	23.0	41.0
36-37	35.51595579941384	40.0	37.0	41.0	22.0	41.0
38-39	35.419130690472976	40.0	36.5	41.0	21.0	41.0
40-41	35.29092211692343	40.0	36.0	41.0	18.5	41.0
42-43	35.16137648709099	40.0	36.0	41.0	17.0	41.0
44-45	35.020059158263116	40.0	35.5	41.0	15.0	41.0
46-47	34.827306135877215	40.0	35.0	41.0	11.0	41.0
48-49	34.63409457368452	40.0	35.0	41.0	8.5	41.0
50-51	34.45650831276633	40.0	35.0	41.0	7.0	41.0
52-53	34.265547572450494	39.0	35.0	41.0	3.5	41.0
54-55	34.06250753186279	39.0	35.0	41.0	2.0	41.0
56-57	33.816539789927305	39.0	35.0	41.0	2.0	41.0
58-59	33.748859675973236	39.0	35.0	41.0	2.0	41.0
60-61	33.508989428879936	38.0	35.0	41.0	2.0	41.0
62-63	33.215365482135624	37.0	34.0	40.0	2.0	41.0
64-65	32.9087053872703	37.0	34.0	40.0	2.0	41.0
66-67	32.588734683203825	36.0	34.0	39.0	2.0	41.0
68-69	32.25366138914062	35.5	34.0	39.0	2.0	41.0
70-71	31.924745543547424	35.0	34.0	38.5	2.0	40.5
72-73	31.581429981393285	35.0	33.0	37.0	2.0	39.5
74-75	31.253130844725526	35.0	33.0	37.0	2.0	39.0
76-77	30.957660386499043	35.0	33.0	36.0	2.0	39.0
78-79	30.706996678749782	35.0	33.0	36.0	2.0	37.5
80-81	30.484025521566437	35.0	33.0	35.5	2.0	37.0
82-83	30.28845196720205	35.0	33.0	35.0	2.0	37.0
84-85	30.11925258614041	35.0	33.0	35.0	2.0	36.0
86-87	29.977194122013767	35.0	32.0	35.0	2.0	36.0
88-89	29.860282740103735	35.0	32.0	35.0	2.0	36.0
90-91	29.763711003027208	35.0	32.0	35.0	2.0	35.0
92-93	29.701276680870755	35.0	32.0	35.0	2.0	35.0
94-95	29.630414204249657	35.0	32.0	35.0	2.0	35.0
96-97	29.565948388060853	35.0	32.0	35.0	2.0	35.0
98-99	29.511392093110693	35.0	32.0	35.0	2.0	35.0
100-101	29.460361915045407	35.0	32.0	35.0	2.0	35.0
102-103	28.784683023060758	34.0	30.0	35.0	2.0	35.0
104-105	29.33323913483601	35.0	31.5	35.0	2.0	35.0
106-107	29.396504974644735	35.0	32.0	35.0	2.0	35.0
108-109	29.467356605383415	35.0	32.0	35.0	2.0	35.0
110-111	29.578411813335133	35.0	33.0	35.0	2.0	35.0
112-113	29.544569044887492	35.0	33.0	35.0	2.0	35.0
114-115	29.48875251865492	35.0	32.5	35.0	2.0	35.0
116-117	29.432301207026203	35.0	32.0	35.0	2.0	35.0
118-119	29.35743659979176	35.0	32.0	35.0	2.0	35.0
120-121	29.26523906735052	35.0	32.0	35.0	2.0	35.0
122-123	29.15589600244876	35.0	32.0	35.0	2.0	35.0
124-125	28.63946750332607	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	109793.0
3	6976.0
4	5163.0
5	4839.0
6	4903.0
7	5043.0
8	5307.0
9	5537.0
10	5558.0
11	5546.0
12	6029.0
13	6904.0
14	7803.0
15	6862.0
16	4795.0
17	4198.0
18	3932.0
19	3584.0
20	3463.0
21	3655.0
22	4173.0
23	5085.0
24	6806.0
25	8676.0
26	10651.0
27	12384.0
28	14439.0
29	17558.0
30	21062.0
31	26156.0
32	33490.0
33	45431.0
34	68682.0
35	119465.0
36	273226.0
37	583473.0
38	198721.0
39	248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.584333305514335	17.394315005448895	11.668922424897765	27.352429264139005
2	15.978756531631413	18.957397373850338	37.91533704182172	27.14850905269653
3	19.37954321963635	22.464292944873996	28.07402435262133	30.082139482868325
4	13.632071065469786	15.215314753377147	31.230839231851697	39.92177494930137
5	14.585720339648109	36.84521574512628	32.04629172406549	16.52277219116012
6	35.027385161673436	32.91572845735782	16.335542835095207	15.721343545873538
7	30.803534297269113	31.11200178402454	18.374006280248558	19.710457638457783
8	28.14795933125072	34.76874349184311	17.49856579505573	19.584731381850435
9	27.602391829262483	15.922248073647033	17.859538181469773	38.61582191562071
10-11	27.082382410158285	24.73885578295361	25.355460731881696	22.823301075006412
12-13	26.70217531412462	22.003072365172546	26.159688229694268	25.13506409100857
14-15	24.115789435628482	23.55743738310549	23.483504617935715	28.84326856333031
16-17	24.49885997724775	26.632666833773595	24.14703160249118	24.721441586487476
18-19	24.589543605267725	25.756379789059636	25.69034041609625	23.963736189576384
20-21	25.261084491834257	24.763680273027013	25.52234974837553	24.452885486763204
22-23	25.164797157896768	24.273356005244587	25.405666129996334	25.15618070686231
24-25	24.714120615853304	24.780943302547094	25.313717149027244	25.191218932572358
26-27	24.416551780652874	24.902959479783277	25.563051934905424	25.11743680465843
28-29	24.505849545919055	25.307902550951546	25.338451786437343	24.847796116692052
30-31	25.095534147658256	24.96902898019783	25.31172873725006	24.623708134893853
32-33	24.71656093939803	24.993914254863775	25.319863149065807	24.969661656672386
34-35	24.73665594932804	25.137290794979076	25.567149268264465	24.55890398742842
36-37	24.847765989240887	25.11246577521547	25.489932610917226	24.54983562462642
38-39	24.766271233827585	25.282535237066888	25.502465630603705	24.448727898501822
40-41	24.662512292000077	25.199021942425237	25.470861934326976	24.66760383124771
42-43	24.57924001696778	25.23143907988354	25.251835364325242	24.937485538823438
44-45	24.4404729768814	25.29600220773962	25.47688742456086	24.786637390818118
46-47	24.534289859822994	25.293140099878524	25.108037039893567	25.064533000404914
48-49	24.464725575072787	25.45266495382064	25.1011077261246	24.98150174498197
50-51	24.372927231359544	25.533135375894183	25.25352250159073	24.840414891155543
52-53	24.625817056475714	25.410426267281107	25.15988638335615	24.80387029288703
54-55	24.367805564660742	25.075650029886432	25.40497319861944	25.15157120683339
56-57	24.37262595684785	25.345320845303977	25.130542245917127	25.15151095193105
58-59	24.485393006575016	25.42220610068835	25.307691658793356	24.78470923394327
60-61	24.811372028228217	25.160488932379536	25.367313884657655	24.66082515473459
62-63	24.580204095405204	25.262379972234537	25.403707845670326	24.753708086689933
64-65	24.452795104409695	25.047932774810562	25.30766153134219	25.19161058943756
66-67	24.350602790043	25.391114571081502	25.196340599271156	25.06194203960434
68-69	24.260702475753426	25.238127374043152	25.318838815726046	25.182331334477375
70-71	24.2716688679791	25.133826138094594	25.479478385361432	25.11502660856487
72-73	24.364612054836783	25.3681574532904	25.14307526560361	25.124155226269206
74-75	24.1985495439909	25.470108748047743	25.220804089620728	25.11053761834063
76-77	24.29107094653221	25.08932789271735	25.288169070435572	25.33143209031487
78-79	24.110245984613307	25.125059049804292	25.40916091433199	25.355534051250412
80-81	24.257057054161926	25.366470316024913	25.060435667045873	25.316036962767292
82-83	24.345390740990688	25.417385708501243	25.142593226384896	25.09463032412317
84-85	24.34620418217226	25.44549462044232	24.93215297996645	25.27614821741897
86-87	23.959578601315005	25.5915525037117	25.204565393440408	25.244303501532883
88-89	23.98389145440873	25.314319698050635	25.174076412856948	25.527712434683686
90-91	24.148422688080913	25.136003215210305	25.25359097596034	25.46198312074844
92-93	24.107227860106555	25.42043111437293	25.269551929795387	25.20278909572513
94-95	24.15002047163391	25.209152612942713	25.13045959194716	25.510367323476217
96-97	23.920021957025327	25.220963656724443	25.184448954887173	25.674565431363057
98-99	23.848438433950985	25.25400454080944	25.10704283400497	25.7905141912346
100-101	23.86356241443804	25.24758739371035	25.231619844590558	25.657230347261052
102-103	23.668487168116	25.21480872683802	25.287295374351658	25.829408730694333
104-105	23.81343033569211	25.245900256444862	25.273286109557873	25.66738329830515
106-107	23.711906416765107	25.1433541604717	25.314689233366682	25.83005018939651
108-109	23.780183772700063	25.11875570354262	25.321514854894644	25.779545668862674
110-111	23.6116124017462	25.45741305913154	25.215608533355827	25.715366005766434
112-113	23.530820679681852	25.518408050132564	25.298535791757047	25.652235478428537
114-115	23.56195130181187	25.482703767752664	25.1813679117383	25.773977018697163
116-117	23.358084262422114	25.310420998782234	25.320875318826637	26.010619419969018
118-119	23.469753897074042	25.22055626907639	25.32591231496788	25.98377751888169
120-121	23.378976449357232	25.297704588112303	25.4102306860326	25.913088276497863
122-123	23.288117373497446	25.42837361735512	25.218954549645062	26.06455445950237
124-125	23.12006097799791	25.370372044118668	25.201778725788813	26.30778825209461
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20.0
1	24.5
2	38.5
3	49.5
4	50.0
5	59.5
6	118.0
7	319.5
8	478.5
9	550.0
10	547.0
11	467.5
12	454.0
13	475.5
14	510.5
15	574.0
16	642.5
17	673.5
18	722.0
19	800.5
20	859.5
21	882.0
22	968.0
23	1128.0
24	1321.0
25	1542.0
26	1885.5
27	2364.0
28	2943.0
29	3775.5
30	5080.0
31	6887.0
32	9080.5
33	11719.5
34	14452.5
35	17006.0
36	20259.5
37	23549.0
38	26699.0
39	30226.0
40	33659.0
41	37008.0
42	40761.0
43	45940.0
44	51167.0
45	56517.5
46	60601.0
47	64445.5
48	70030.5
49	74185.0
50	76531.0
51	77897.5
52	77957.5
53	76998.0
54	76802.5
55	75097.0
56	70586.0
57	64717.5
58	59444.0
59	54041.0
60	46735.0
61	39303.0
62	32155.5
63	24870.0
64	18557.5
65	14041.5
66	10815.0
67	8519.0
68	6638.0
69	5166.0
70	4021.0
71	3113.0
72	2365.5
73	1886.0
74	1492.0
75	1140.0
76	871.0
77	609.0
78	425.5
79	322.0
80	264.5
81	196.0
82	165.5
83	143.5
84	132.0
85	127.5
86	109.0
87	95.0
88	93.5
89	90.5
90	80.0
91	78.5
92	81.5
93	70.0
94	61.0
95	60.5
96	55.0
97	47.0
98	28.5
99	10.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.36574725719684553
2	0.0
3	0.0
4	0.01813672560399514
5	0.18546458939899352
6	0.05248201993714209
7	0.02687368644312901
8	0.009640784374216687
9	0.00801390201106762
10-11	0.0026813431540790157
12-13	0.057784451342961264
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	2.711470605248443E-4
92-93	6.025490233885429E-4
94-95	1.5063725584713573E-4
96-97	6.3267647455797E-4
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0
106-107	1.5063725584713573E-4
108-109	6.929313768968243E-4
110-111	6.628039257273972E-4
112-113	9.640784374216687E-4
114-115	3.6152941403312574E-4
116-117	9.038235350828143E-4
118-119	3.314019628636986E-4
120-121	6.0254902338854294E-5
122-123	0.0015063725584713572
124-125	6.0254902338854294E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1659616.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.15794014932536
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.17773195192595	54.196896802802186
2	9.194570017153172	11.981984857528955
3	2.9783450403912246	5.821884836575543
4	1.4301542406944172	3.7274361767788275
5	0.8351158221334053	2.720721337816153
6	0.5120156213807214	2.001712992806681
7	0.354964331397879	1.6190121262257802
8	0.2481761719697767	1.293651852775631
9	0.20656575204996774	1.2113459018074961
>10	0.9985879116048362	11.274353859826551
>50	0.04775765956358879	2.12791013188597
>100	0.015089904880285514	1.5305491871900179
>500	7.404598838733342E-4	0.3312748706182479
>1k	1.8511497096833354E-4	0.16126506536195065
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	6.025490233885429E-5
5	0.0	6.025490233885429E-5	0.0	0.0	6.025490233885429E-5
6	0.0	6.025490233885429E-5	0.0	0.0	1.2050980467770857E-4
7	0.0	6.025490233885429E-5	0.0	0.0	1.2050980467770857E-4
8	0.0	6.025490233885429E-5	0.0	0.0	1.2050980467770857E-4
9	0.0	6.025490233885429E-5	0.0	6.025490233885429E-5	1.2050980467770857E-4
10-11	0.0	6.025490233885429E-5	0.0	6.025490233885429E-5	1.2050980467770857E-4
12-13	0.0	6.025490233885429E-5	0.0	1.8076470701656287E-4	1.2050980467770857E-4
14-15	0.0	1.2050980467770857E-4	0.0	1.8076470701656287E-4	2.1089215818599E-4
16-17	0.0	1.2050980467770857E-4	0.0	1.8076470701656287E-4	2.4101960935541715E-4
18-19	0.0	1.2050980467770857E-4	0.0	2.4101960935541715E-4	3.0127451169427146E-4
20-21	0.0	1.2050980467770857E-4	0.0	2.711470605248443E-4	3.0127451169427146E-4
22-23	0.0	1.8076470701656287E-4	0.0	4.2178431637198E-4	3.6152941403312574E-4
24-25	0.0	2.4101960935541715E-4	0.0	6.3267647455797E-4	4.2178431637198E-4
26-27	0.0	2.4101960935541715E-4	0.0	0.001024333339760523	4.2178431637198E-4
28-29	0.0	2.4101960935541715E-4	0.0	0.002560833349401307	4.2178431637198E-4
30-31	0.0	2.4101960935541715E-4	0.0	0.008495941229778455	4.2178431637198E-4
32-33	0.0	2.4101960935541715E-4	0.0	0.020275774637024468	4.2178431637198E-4
34-35	0.0	2.4101960935541715E-4	0.0	0.035309372770568614	4.2178431637198E-4
36-37	0.0	2.4101960935541715E-4	0.0	0.057151774868403296	4.5191176754140716E-4
38-39	0.0	2.4101960935541715E-4	0.0	0.09429892216030697	4.820392187108343E-4
40-41	0.0	2.4101960935541715E-4	0.0	0.13861640283053428	4.820392187108343E-4
42-43	0.0	2.4101960935541715E-4	0.0	0.18025254034668262	5.121666698802615E-4
44-45	0.0	2.4101960935541715E-4	0.0	0.22523282494263736	5.724215722191158E-4
46-47	0.0	2.4101960935541715E-4	0.0	0.2759975801631221	6.025490233885429E-4
48-49	0.0	2.4101960935541715E-4	0.0	0.333269864836203	6.025490233885429E-4
50-51	0.0	2.4101960935541715E-4	0.0	0.3929523456028382	6.025490233885429E-4
52-53	0.0	2.4101960935541715E-4	0.0	0.45254444401596516	6.3267647455797E-4
54-55	0.0	2.4101960935541715E-4	0.0	0.5224100032778667	6.628039257273972E-4
56-57	0.0	2.4101960935541715E-4	0.0	0.5944146115727975	6.929313768968243E-4
58-59	0.0	2.4101960935541715E-4	0.0	0.6768433179723503	7.230588280662515E-4
60-61	0.0	2.4101960935541715E-4	0.0	0.7649661126429246	7.230588280662515E-4
62-63	0.0	2.711470605248443E-4	0.0	0.8620668877619884	7.230588280662515E-4
64-65	0.0	3.0127451169427146E-4	0.0	0.9575106530667336	7.230588280662515E-4
66-67	0.0	3.0127451169427146E-4	0.0	1.068349545919056	7.531862792356786E-4
68-69	0.0	3.0127451169427146E-4	0.0	1.1906669976669302	7.833137304051058E-4
70-71	0.0	3.0127451169427146E-4	0.0	1.3156055376665445	7.833137304051058E-4
72-73	0.0	3.0127451169427146E-4	0.0	1.4368986560746584	9.942058885910958E-4
74-75	0.0	3.0127451169427146E-4	0.0	1.5656332549216203	0.001024333339760523
76-77	0.0	3.0127451169427146E-4	0.0	1.7196146578485627	0.001024333339760523
78-79	0.0	3.0127451169427146E-4	0.0	1.8881476196903382	0.0011147156932688044
80-81	0.0	3.0127451169427146E-4	0.0	2.0605368952818	0.0011448431444382315
82-83	0.0	3.0127451169427146E-4	0.0	2.238349112083759	0.0012050980467770858
84-85	0.0	3.0127451169427146E-4	0.0	2.427579632878931	0.0012050980467770858
86-87	0.0	3.0127451169427146E-4	0.0	2.639134594990648	0.0012050980467770858
88-89	0.0	3.0127451169427146E-4	0.0	2.8829560573048223	0.0012050980467770858
90-91	0.0	3.0127451169427146E-4	0.0	3.1371413628212794	0.0012050980467770858
92-93	0.0	3.0127451169427146E-4	0.0	3.379667344735168	0.0012050980467770858
94-95	0.0	3.0127451169427146E-4	0.0	3.6324969149490003	0.0012050980467770858
96-97	0.0	3.0127451169427146E-4	0.0	3.9267517305207953	0.00126535294911594
98-99	0.0	3.0127451169427146E-4	0.0	4.262883703218094	0.00126535294911594
100-101	0.0	3.6152941403312574E-4	0.0	4.610102577945741	0.00126535294911594
102-103	0.0	3.6152941403312574E-4	0.0	4.96518471742861	0.0013256078514547944
104-105	0.0	3.6152941403312574E-4	0.0	5.327135915778108	0.0013557353026242215
106-107	0.0	3.6152941403312574E-4	0.0	5.715237741742668	0.0014159902049630758
108-109	0.0	3.6152941403312574E-4	0.0	6.144132136590633	0.0015365000096407844
110-111	0.0	3.6152941403312574E-4	0.0	6.599839962979388	0.0018377745213350558
112-113	0.0	3.6152941403312574E-4	0.0	7.056632377610242	0.0019281568748433372
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1935	0.0	63.015713	1
GTATCAA	3160	0.0	52.20615	1
GTACATG	7560	0.0	39.84817	1
TACATGG	7940	0.0	38.28498	2
TCAACGC	4155	0.0	37.871426	4
ACATGGG	7955	0.0	37.38859	3
TATCAAC	4535	0.0	36.538124	2
CAACGCA	4370	0.0	35.462605	5
ATCAACG	4500	0.0	35.23286	3
CATGGGG	7500	0.0	35.126896	4
AACGCAG	4550	0.0	34.04736	6
ATGGGGG	4705	0.0	32.684273	5
CGTATAC	145	4.013147E-6	28.774567	3
GTGGTAT	940	0.0	28.614378	1
ACGCAGA	5455	0.0	28.50029	7
CGCAGAG	5790	0.0	26.435808	8
TGGGGGG	3240	0.0	24.458403	6
GCAGAGT	6445	0.0	23.102295	9
GAGTACT	2695	0.0	22.63714	12-13
AGAGTAC	5965	0.0	22.61223	10-11
>>END_MODULE