FastQCFastQC Report
Mon 15 Aug 2016
SRR2921284_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921284_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1794657
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15350.054.2761761
GTATCAA27300.046.6488271
GTACATG88550.038.6428261
TCAACGC33000.037.8668064
TACATGG91600.037.288052
CAACGCA34400.036.1527335
ACATGGG93850.035.6966443
ATCAACG36800.033.7949453
AACGCAG37450.033.3654146
TATCAAC37250.033.2269362
CATGGGG83800.032.5218124
ATGGGGG50700.029.811175
ACGCAGA46900.026.511237
CGCAGAG49200.025.2718858
TGGGGGG34350.023.3849326
GTGGTAT10150.021.6932831
GAGTACA59850.021.6761381
GTATAGG4950.021.6399821
AGAGTAC51500.021.02396210-11
GTAAGTA8800.020.9619792
Produced by FastQC (version 0.11.5)