FastQCFastQC Report
Mon 15 Aug 2016
SRR2921284_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921284_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1794657
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19030.10603697531060252No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22100.061.3707471
GTATCAA34350.049.2252041
GTACATG88000.037.2749861
TACATGG91750.035.3319632
ACATGGG92750.034.1800463
TATCAAC49250.033.7577062
TCAACGC48050.033.484614
CAACGCA49650.032.2855345
ATCAACG50650.032.2363623
CATGGGG83100.031.336854
ATGGGGG48650.030.9893575
AACGCAG52550.030.3845066
GTGGTAT12400.025.055871
ACGCAGA66150.023.5913337
TGGTATC12550.022.3166522
CGCAGAG71050.022.1283078
TGGGGGG35000.021.6185116
AGAGTAC71700.021.110065
AGTACAT60550.020.4611597
CAGAGTA76800.020.2513624
Produced by FastQC (version 0.11.5)