##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921284_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1794657
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.276084510856393	31.0	30.0	34.0	19.0	34.0
2	30.0553024895565	33.0	31.0	34.0	26.0	34.0
3	30.291637343514665	33.0	31.0	34.0	27.0	34.0
4	32.47705271815171	37.0	35.0	37.0	19.0	37.0
5	32.15319417582301	37.0	33.0	37.0	19.0	37.0
6	32.29452368892775	37.0	35.0	37.0	17.0	37.0
7	32.51775297452382	37.0	35.0	37.0	17.0	37.0
8	32.76297030574645	37.0	35.0	37.0	17.0	37.0
9	34.5119396074013	39.0	37.0	39.0	18.0	39.0
10-11	34.70250554841399	39.0	37.0	39.0	25.5	39.0
12-13	34.73132776903888	39.0	37.0	39.0	25.5	39.0
14-15	36.13845431188244	41.0	38.0	41.0	27.0	41.0
16-17	36.15061596728512	41.0	38.0	41.0	27.0	41.0
18-19	36.172668927822976	41.0	38.0	41.0	27.0	41.0
20-21	36.16666499503805	41.0	38.0	41.0	27.0	41.0
22-23	36.11787182731854	41.0	38.0	41.0	26.0	41.0
24-25	36.06493831411796	41.0	38.0	41.0	25.5	41.0
26-27	35.970105986826454	40.5	38.0	41.0	25.0	41.0
28-29	35.902375217102765	40.0	38.0	41.0	25.0	41.0
30-31	35.81690205983651	40.0	37.5	41.0	24.0	41.0
32-33	35.70292345556839	40.0	37.0	41.0	24.0	41.0
34-35	35.62384678520743	40.0	37.0	41.0	23.5	41.0
36-37	35.54490579536926	40.0	37.0	41.0	23.0	41.0
38-39	35.45244355885275	40.0	37.0	41.0	22.0	41.0
40-41	35.32577283570063	40.0	36.0	41.0	20.0	41.0
42-43	35.18771247096242	40.0	36.0	41.0	18.5	41.0
44-45	35.045645491032545	40.0	35.5	41.0	15.5	41.0
46-47	34.83974375047711	40.0	35.0	41.0	11.5	41.0
48-49	34.63907810796158	40.0	35.0	41.0	8.5	41.0
50-51	34.46216992996433	40.0	35.0	41.0	7.0	41.0
52-53	34.2783501248428	39.5	35.0	41.0	4.0	41.0
54-55	34.07729972914044	39.0	35.0	41.0	2.0	41.0
56-57	33.833338905428725	39.0	35.0	41.0	2.0	41.0
58-59	33.76651973051118	39.0	35.0	41.0	2.0	41.0
60-61	33.52858122749919	38.0	35.0	41.0	2.0	41.0
62-63	33.24483034919764	37.0	34.0	40.0	2.0	41.0
64-65	32.94271300866962	37.0	34.0	40.0	2.0	41.0
66-67	32.62370107491292	36.0	34.0	39.0	2.0	41.0
68-69	32.29102636325493	36.0	34.0	39.0	2.0	41.0
70-71	31.953917935293482	35.0	34.0	38.5	2.0	40.5
72-73	31.612334000313155	35.0	33.0	37.0	2.0	39.5
74-75	31.280060479523385	35.0	33.0	37.0	2.0	39.0
76-77	30.98204113655144	35.0	33.0	36.5	2.0	39.0
78-79	30.722696871881368	35.0	33.0	36.0	2.0	37.5
80-81	30.492048062666015	35.0	33.0	35.5	2.0	37.0
82-83	30.29430442697407	35.0	33.0	35.0	2.0	37.0
84-85	30.11892049567132	35.0	32.5	35.0	2.0	36.0
86-87	29.974825830228284	35.0	32.0	35.0	2.0	36.0
88-89	29.854687553109034	35.0	32.0	35.0	2.0	36.0
90-91	29.7522381714166	35.0	32.0	35.0	2.0	35.5
92-93	29.68406163406155	35.0	32.0	35.0	2.0	35.0
94-95	29.611730820986963	35.0	32.0	35.0	2.0	35.0
96-97	29.548321489844575	35.0	32.0	35.0	2.0	35.0
98-99	29.492431144224216	35.0	32.0	35.0	2.0	35.0
100-101	29.4374468213146	35.0	32.0	35.0	2.0	35.0
102-103	28.76639185092193	34.0	30.0	35.0	2.0	35.0
104-105	29.315989907820825	35.0	31.5	35.0	2.0	35.0
106-107	29.378422729245756	35.0	32.0	35.0	2.0	35.0
108-109	29.454086769783864	35.0	32.0	35.0	2.0	35.0
110-111	29.565547344144314	35.0	32.5	35.0	2.0	35.0
112-113	29.526791749064028	35.0	32.5	35.0	2.0	35.0
114-115	29.472703976302995	35.0	32.0	35.0	2.0	35.0
116-117	29.42041654756313	35.0	32.0	35.0	2.0	35.0
118-119	29.351363519602913	35.0	32.0	35.0	2.0	35.0
120-121	29.265281610915068	35.0	32.0	35.0	2.0	35.0
122-123	29.159253829561862	35.0	32.0	35.0	2.0	35.0
124-125	28.635311650081324	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	117012.0
3	7238.0
4	5506.0
5	5205.0
6	5092.0
7	5499.0
8	5914.0
9	5922.0
10	5776.0
11	6038.0
12	6719.0
13	7985.0
14	9133.0
15	8133.0
16	5384.0
17	4891.0
18	4331.0
19	3865.0
20	3696.0
21	3865.0
22	4405.0
23	5507.0
24	7450.0
25	9609.0
26	11464.0
27	13232.0
28	15785.0
29	18880.0
30	22912.0
31	27950.0
32	36469.0
33	49226.0
34	74686.0
35	128616.0
36	294981.0
37	628169.0
38	217859.0
39	253.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.88585748343897	18.072770131548932	12.232686398260924	26.808685986751176
2	15.856288973324709	19.095570908535723	38.05763441147807	26.990505706661494
3	19.550922543973584	22.520403620301817	27.95598267524101	29.97269116048359
4	13.51133245203186	15.789693677144939	31.197013235126924	39.50196063569628
5	14.536577577780422	37.347385250399554	32.279383729764646	15.836653442055383
6	35.051052122502796	33.60838018569983	15.738046723214708	15.602520968582667
7	30.758838250129582	31.093696877142328	18.329775553313752	19.81768931941433
8	27.992650861300334	35.2824971468312	17.400428866935343	19.32442312493313
9	27.249687522115856	16.37311670429032	17.664153283845828	38.713042489748
10-11	27.254982266391398	25.60267801171839	25.011297687804905	22.131042034085304
12-13	26.67633416655586	22.563855537222246	26.195663172930335	24.564147123291566
14-15	24.1894412135578	23.801205467117114	23.3388887124392	28.670464606885883
16-17	24.454143605156865	26.998223058779473	23.905347929994424	24.642285406069238
18-19	24.512427722957646	26.045255444355103	25.751912482440936	23.690404350246315
20-21	25.260871576017035	24.94103330051369	25.65818983794675	24.139905285522527
22-23	25.020017752695917	24.57809486715289	25.636430805440817	24.765456574710377
24-25	24.630806889561626	25.03383654926819	25.496571211100505	24.838785350069678
26-27	24.389367996224347	25.07272977510466	25.761106439837807	24.776795788833187
28-29	24.447958579271695	25.476121621011703	25.532845552102714	24.543074247613887
30-31	24.918577756083753	25.250173152864306	25.475146504318097	24.356102586733844
32-33	24.47629268434024	25.303943873397532	25.598512696297952	24.62125074596427
34-35	24.581661008203795	25.423688203372567	25.7527761572267	24.241874631196936
36-37	24.74107865737018	25.289902193009585	25.73583698723489	24.233182162385347
38-39	24.639387916465374	25.464503802119292	25.63083084957181	24.265277431843522
40-41	24.546222481510394	25.396106331181944	25.614866796273606	24.442804391034052
42-43	24.439906901430188	25.50125177122982	25.45823519480324	24.600606132536747
44-45	24.382570039846055	25.44650593400299	25.744362293184714	24.426561732966242
46-47	24.339915649619957	25.492670744326073	25.32481137064074	24.84260223541323
48-49	24.40870316723474	25.66195100233638	25.222396257334967	24.70694957309391
50-51	24.34456834927231	25.762248719393178	25.269954091506065	24.623228839828446
52-53	24.501255671696597	25.73550266151136	25.28719972674444	24.476041940047597
54-55	24.25154221670213	25.335649096178265	25.486625020825706	24.926183666293895
56-57	24.26747840952338	25.585641155942334	25.251844781481918	24.895035653052368
58-59	24.416448379829685	25.622444846006786	25.33854658578213	24.6225601883814
60-61	24.637103357354636	25.415692246484983	25.485733485562978	24.461470910597402
62-63	24.459715700548905	25.45422328612097	25.553963793638562	24.53209721969156
64-65	24.374295478188866	25.259589994076865	25.473892782854886	24.892221744879382
66-67	24.264163012765113	25.65671323266786	25.35102807946031	24.728095675106722
68-69	24.187713863986264	25.482780275005197	25.441574629581027	24.88793123142751
70-71	24.17590102175513	25.38365269798073	25.548085232999956	24.892361047264185
72-73	24.32044117622476	25.63428554871488	25.212533648491046	24.83273962656931
74-75	24.04595975721266	25.70566409068697	25.37983581263718	24.868540339463195
76-77	24.177684092280586	25.28413507427882	25.421765830462313	25.116415002978286
78-79	24.093211126137195	25.35813250108517	25.5736890113264	24.97496736145124
80-81	24.27004157340372	25.56020454047765	25.184756752961707	24.984997133156924
82-83	24.259175987389234	25.59639530004898	25.313750761287533	24.830677951274254
84-85	24.304700006742237	25.66465346860152	25.128590031409903	24.90205649324634
86-87	23.990350245200055	25.83708196050833	25.22417932786042	24.94838846643119
88-89	23.999906388797413	25.529056527236122	25.318096995693328	25.152940088273134
90-91	24.094010347958534	25.40651997725466	25.38197484918398	25.117494825602826
92-93	24.108627430002986	25.656061801534342	25.36241090174786	24.87289986671481
94-95	24.097675516178167	25.496243988752166	25.229061940265453	25.17701855480422
96-97	23.874117967947484	25.469500016298458	25.285508555017877	25.37087346073618
98-99	23.86191344641344	25.499106514503882	25.222758443535447	25.416221595547228
100-101	23.89384155300985	25.47929771538517	25.360472781149824	25.266387950455155
102-103	23.721858828734405	25.42145936521575	25.432464253615034	25.424217552434808
104-105	23.756935169227322	25.504400005126328	25.34768482222508	25.390980003421266
106-107	23.80450626749639	25.38397330741936	25.358480956796175	25.453039468288075
108-109	23.782518911786603	25.35578007497849	25.438748991445674	25.42295202178923
110-111	23.662531318789544	25.64475681741755	25.322465473063126	25.37024639072978
112-113	23.722662003894374	25.685924284000055	25.283559576416557	25.307854135689013
114-115	23.703258347579364	25.65450014821815	25.260746930324096	25.38149457387839
116-117	23.441786627817727	25.52551523464131	25.452380932480462	25.580317205060503
118-119	23.598620679279513	25.428530274540766	25.402842836060373	25.570006210119345
120-121	23.445697516877196	25.468954905272906	25.537101688875662	25.548245888974236
122-123	23.41299946423661	25.60648109323592	25.335674012912374	25.644845429615092
124-125	23.354475732396626	25.496306813116277	25.239628095857924	25.909589358629177
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	32.0
1	39.5
2	49.5
3	54.5
4	57.0
5	65.5
6	150.0
7	385.0
8	560.0
9	638.5
10	656.5
11	576.0
12	581.5
13	640.5
14	704.5
15	760.0
16	789.5
17	852.0
18	942.0
19	976.5
20	1004.0
21	1095.0
22	1231.0
23	1408.5
24	1624.5
25	1906.5
26	2274.5
27	2838.0
28	3538.5
29	4550.5
30	6179.0
31	7993.5
32	10476.5
33	13433.0
34	16355.5
35	19614.5
36	23484.0
37	27277.0
38	30921.5
39	34882.0
40	38583.5
41	41824.5
42	45633.0
43	50780.5
44	55847.5
45	61725.5
46	66222.0
47	70154.0
48	75904.5
49	79806.0
50	82295.5
51	84703.5
52	84375.5
53	82584.0
54	82337.0
55	80196.0
56	75480.0
57	69374.0
58	63011.5
59	56556.5
60	48456.5
61	40322.0
62	33074.5
63	25644.0
64	18735.5
65	13890.0
66	10559.5
67	8068.0
68	6211.5
69	4720.0
70	3571.0
71	2783.0
72	2081.0
73	1606.0
74	1244.5
75	879.0
76	651.5
77	460.0
78	338.5
79	265.5
80	218.5
81	203.5
82	197.5
83	173.0
84	139.0
85	114.0
86	118.5
87	114.5
88	102.0
89	96.5
90	91.5
91	83.5
92	78.5
93	84.0
94	85.0
95	77.5
96	57.5
97	44.5
98	29.0
99	8.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.34106795894703
2	0.0
3	0.0
4	0.019000845286870974
5	0.18387914793746102
6	0.05026030043623935
7	0.026021685480846758
8	0.00897107358119128
9	0.007243724009657555
10-11	0.002340280064658595
12-13	0.05675179156797093
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	1.950233387215496E-4
92-93	5.014885852839847E-4
94-95	2.786047696022137E-5
96-97	4.7362810832376323E-4
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0
106-107	5.572095392044274E-5
108-109	5.014885852839847E-4
110-111	4.179071544033205E-4
112-113	9.193957396873052E-4
114-115	2.7860476960221367E-4
116-117	7.52232877925977E-4
118-119	3.0646524656243503E-4
120-121	8.35814308806641E-5
122-123	0.0011980005092895188
124-125	5.572095392044274E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1794657.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.47467492269929
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.18983385016288	52.169729855897565
2	9.70812995176467	12.324407855911655
3	3.1846141134748396	6.064270368211669
4	1.477961471615063	3.752524958361616
5	0.8782474779034686	2.78732365808016
6	0.5370171713930171	2.045219422924755
7	0.38143635744603704	1.6948084154810015
8	0.27889590009122533	1.416226127645132
9	0.20497018840074402	1.170937446982349
>10	1.0871550710147129	11.975587281932528
>50	0.05367559847857696	2.3063338633129984
>100	0.016835671731739204	1.6422105774654578
>500	9.642101252543493E-4	0.38997390556562195
>1k	2.629663977966404E-4	0.2604462622275181
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1903	0.10603697531060252	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	5.5720953920442733E-5	0.0	0.0	0.0
6	0.0	5.5720953920442733E-5	0.0	0.0	5.5720953920442733E-5
7	0.0	5.5720953920442733E-5	0.0	0.0	5.5720953920442733E-5
8	0.0	5.5720953920442733E-5	0.0	0.0	5.5720953920442733E-5
9	0.0	5.5720953920442733E-5	0.0	0.0	5.5720953920442733E-5
10-11	0.0	5.5720953920442733E-5	0.0	0.0	1.1144190784088547E-4
12-13	0.0	5.5720953920442733E-5	0.0	5.5720953920442733E-5	2.2288381568177093E-4
14-15	0.0	5.5720953920442733E-5	0.0	5.5720953920442733E-5	3.0646524656243503E-4
16-17	0.0	5.5720953920442733E-5	0.0	5.5720953920442733E-5	3.343257235226564E-4
18-19	0.0	5.5720953920442733E-5	0.0	5.5720953920442733E-5	3.343257235226564E-4
20-21	0.0	5.5720953920442733E-5	0.0	8.35814308806641E-5	3.343257235226564E-4
22-23	0.0	5.5720953920442733E-5	0.0	1.3930238480110683E-4	5.014885852839846E-4
24-25	0.0	5.5720953920442733E-5	0.0	3.6218620048287777E-4	6.129304931248701E-4
26-27	0.0	5.5720953920442733E-5	0.0	6.686514470453128E-4	6.686514470453128E-4
28-29	0.0	5.5720953920442733E-5	0.0	0.0013651633710508471	6.686514470453128E-4
30-31	0.0	5.5720953920442733E-5	0.0	0.005237769668521617	6.686514470453128E-4
32-33	0.0	5.5720953920442733E-5	0.0	0.015044657558519539	6.686514470453128E-4
34-35	0.0	5.5720953920442733E-5	0.0	0.02610526691172742	6.686514470453128E-4
36-37	0.0	5.5720953920442733E-5	0.0	0.043239460242263564	6.686514470453128E-4
38-39	0.0	5.5720953920442733E-5	0.0	0.07550189256219991	7.243724009657555E-4
40-41	0.0	5.5720953920442733E-5	0.0	0.11367074599770319	7.243724009657555E-4
42-43	0.0	1.1144190784088547E-4	0.0	0.14885852839846278	7.52232877925977E-4
44-45	0.0	1.1144190784088547E-4	0.0	0.18822538234325556	7.800933548861983E-4
46-47	0.0	1.1144190784088547E-4	0.0	0.2277593991498097	8.079538318464196E-4
48-49	0.0	1.671628617613282E-4	0.0	0.27659881526107777	8.915352627270837E-4
50-51	0.0	1.671628617613282E-4	0.0	0.32772279048308395	8.915352627270837E-4
52-53	0.0	1.671628617613282E-4	0.0	0.3817721157859134	9.19395739687305E-4
54-55	0.0	1.9502333872154957E-4	0.0	0.43947116357053184	9.472562166475265E-4
56-57	0.0	2.2288381568177093E-4	0.0	0.5019622133923084	9.472562166475265E-4
58-59	0.0	2.2288381568177093E-4	0.0	0.5735914996570375	9.751166936077479E-4
60-61	0.0	2.2288381568177093E-4	0.0	0.6474496240785843	0.0010029771705679692
62-63	0.0	2.2288381568177093E-4	0.0	0.7318111483141347	0.0011701400323292975
64-65	0.0	2.2288381568177093E-4	0.0	0.8092632742635502	0.0011701400323292975
66-67	0.0	2.2288381568177093E-4	0.0	0.8965780090568838	0.0012258609862497404
68-69	0.0	2.2288381568177093E-4	0.0	0.9992717271322599	0.001281581940170183
70-71	0.0	2.2288381568177093E-4	0.0	1.110685774496185	0.001281581940170183
72-73	0.0	2.2288381568177093E-4	0.0	1.2194530765488891	0.0016994890945735033
74-75	0.0	2.2288381568177093E-4	0.0	1.3290561929104001	0.0017273495715337248
76-77	0.0	2.2288381568177093E-4	0.0	1.45740941026614	0.0017552100484939462
78-79	0.0	2.2288381568177093E-4	0.0	1.5996928661019905	0.0017830705254541675
80-81	0.0	2.2288381568177093E-4	0.0	1.7433972062628125	0.0017830705254541675
82-83	0.0	2.2288381568177093E-4	0.0	1.8964348062053085	0.0018387914793746103
84-85	0.0	2.2288381568177093E-4	0.0	2.057384781604507	0.0018387914793746103
86-87	0.0	2.2288381568177093E-4	0.0	2.2354689503342424	0.0018666519563348316
88-89	0.0	2.2288381568177093E-4	0.0	2.441720061270761	0.0019502333872154958
90-91	0.0	2.2288381568177093E-4	0.0	2.6588646186987264	0.0019502333872154958
92-93	0.0	2.2288381568177093E-4	0.0	2.8820548996270596	0.00203381481809616
94-95	0.0	2.2288381568177093E-4	0.0	3.1036292728917	0.0021731172028972667
96-97	0.0	2.2288381568177093E-4	0.0	3.346572631984831	0.002368140541618816
98-99	0.0	2.2288381568177093E-4	0.0	3.615370513697046	0.002563163880340366
100-101	0.0	2.2288381568177093E-4	0.0	3.90904780133474	0.002563163880340366
102-103	0.0	2.2288381568177093E-4	0.0	4.231783566441944	0.002674605788181251
104-105	0.0	2.2288381568177093E-4	0.0	4.561233706496562	0.002813908172982358
106-107	0.0	2.2288381568177093E-4	0.0	4.908430970374841	0.0028417686499425795
108-109	0.0	2.2288381568177093E-4	0.0	5.278139499636978	0.0028417686499425795
110-111	0.0	2.2288381568177093E-4	0.0	5.681280601251381	0.0028417686499425795
112-113	0.0	2.2288381568177093E-4	0.0	6.099243476608622	0.0028417686499425795
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2210	0.0	61.370747	1
GTATCAA	3435	0.0	49.225204	1
GTACATG	8800	0.0	37.274986	1
TACATGG	9175	0.0	35.331963	2
ACATGGG	9275	0.0	34.180046	3
TATCAAC	4925	0.0	33.757706	2
TCAACGC	4805	0.0	33.48461	4
CAACGCA	4965	0.0	32.285534	5
ATCAACG	5065	0.0	32.236362	3
CATGGGG	8310	0.0	31.33685	4
ATGGGGG	4865	0.0	30.989357	5
AACGCAG	5255	0.0	30.384506	6
GTGGTAT	1240	0.0	25.05587	1
ACGCAGA	6615	0.0	23.591333	7
TGGTATC	1255	0.0	22.316652	2
CGCAGAG	7105	0.0	22.128307	8
TGGGGGG	3500	0.0	21.618511	6
AGAGTAC	7170	0.0	21.11006	5
AGTACAT	6055	0.0	20.461159	7
CAGAGTA	7680	0.0	20.251362	4
>>END_MODULE