FastQCFastQC Report
Mon 15 Aug 2016
SRR2921283_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921283_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1346509
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14200.060.7600941
GTATCAA24600.051.762761
TATCACG802.293984E-644.6255872
TCAACGC30400.042.276874
GTACATG70800.041.5176241
CAACGCA31900.040.288935
TACATGG73650.039.1016652
TATCAAC32450.039.055982
ACATGGG74300.038.519353
ATCAACG33800.037.6720923
AACGCAG34300.037.469886
CATGGGG67700.036.298114
ATGGGGG42300.036.1505935
TGGGGGG33800.031.334736
ACGCAGA42050.030.558347
CGCAGAG43950.029.2372748
GCAGAGT49550.024.972489
GTAAGTA10200.023.916982
TATAACG1501.6866543E-423.800312
AGAGTAC44900.022.9876810-11
Produced by FastQC (version 0.11.5)