FastQCFastQC Report
Mon 15 Aug 2016
SRR2921283_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921283_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1346509
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25760.1913095270807696No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20200.15001756393756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19030.14132842780850333No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21200.070.458951
GTATCAA34600.047.833891
GTACATG69850.038.8348351
TACATGG71950.037.4389342
ACATGGG73150.036.906233
CATGGGG65300.034.771844
TCAACGC46500.033.835054
ATGGGGG40200.033.6523675
TATCAAC48450.033.2112962
ATCAACG49450.032.178133
CAACGCA48600.032.0051655
AACGCAG49600.031.2374236
TGGGGGG32900.028.6183596
GTGGTAT9500.027.673311
ACGCAGA59850.025.7804167
TGGTATC10200.024.539462
CGCAGAG64400.024.0479018
AGAGTAC63600.023.08518610-11
GAGTACT34600.022.62334812-13
CAGAGTA67450.022.0773939
Produced by FastQC (version 0.11.5)