##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921283_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1346509
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.99493430790288	31.0	30.0	34.0	16.0	34.0
2	29.78882651359924	33.0	31.0	34.0	25.0	34.0
3	30.021498556637944	33.0	31.0	34.0	26.0	34.0
4	32.14220328271107	37.0	33.0	37.0	19.0	37.0
5	31.817209539631744	37.0	33.0	37.0	19.0	37.0
6	31.95793418387846	37.0	33.0	37.0	17.0	37.0
7	32.1836044170518	37.0	35.0	37.0	17.0	37.0
8	32.426231833578534	37.0	35.0	37.0	17.0	37.0
9	34.14411414999825	39.0	37.0	39.0	17.0	39.0
10-11	34.33520087871674	39.0	37.0	39.0	17.5	39.0
12-13	34.356139840134745	39.0	37.0	39.0	18.5	39.0
14-15	35.72550796169948	40.0	38.0	41.0	22.0	41.0
16-17	35.73590187663061	40.0	38.0	41.0	22.0	41.0
18-19	35.77326182001012	40.5	38.0	41.0	22.5	41.0
20-21	35.769293038516636	40.5	38.0	41.0	22.0	41.0
22-23	35.72485033520013	40.0	38.0	41.0	22.0	41.0
24-25	35.66477424213281	40.5	37.5	41.0	21.5	41.0
26-27	35.56205788449984	40.0	37.0	41.0	19.0	41.0
28-29	35.486290102776884	40.0	37.0	41.0	18.5	41.0
30-31	35.376679249823056	40.0	37.0	41.0	18.0	41.0
32-33	35.2234422495505	40.0	36.0	41.0	16.0	41.0
34-35	35.13144620644942	40.0	36.0	41.0	16.0	41.0
36-37	35.04754219986647	40.0	36.0	41.0	15.0	41.0
38-39	34.947195674147	40.0	35.0	41.0	10.5	41.0
40-41	34.804646682643785	40.0	35.0	41.0	8.5	41.0
42-43	34.649885741573215	40.0	35.0	41.0	6.5	41.0
44-45	34.48355042558201	40.0	35.0	41.0	2.0	41.0
46-47	34.23770431538148	40.0	35.0	41.0	2.0	41.0
48-49	33.99985518106452	40.0	35.0	41.0	2.0	41.0
50-51	33.80746916656331	40.0	35.0	41.0	2.0	41.0
52-53	33.62232929746477	39.0	35.0	41.0	2.0	41.0
54-55	33.41893815785858	39.0	34.0	41.0	2.0	41.0
56-57	33.1812338424771	39.0	34.0	41.0	2.0	41.0
58-59	33.12809494775007	38.5	34.0	41.0	2.0	41.0
60-61	32.888749722430376	38.0	34.0	41.0	2.0	41.0
62-63	32.60425626564694	37.0	34.0	40.0	2.0	41.0
64-65	32.30673058999234	37.0	34.0	40.0	2.0	41.0
66-67	31.99162055359452	36.0	33.0	39.0	2.0	41.0
68-69	31.663502063484167	36.0	33.0	39.0	2.0	41.0
70-71	31.32688121653847	35.0	33.0	38.5	2.0	40.5
72-73	30.986340603739002	35.0	33.0	37.0	2.0	39.5
74-75	30.657025315092582	35.0	32.5	37.0	2.0	39.0
76-77	30.356729513133594	35.0	32.0	36.0	2.0	39.0
78-79	30.097718990366943	35.0	32.0	36.0	2.0	37.5
80-81	29.871893541001214	35.0	32.0	35.5	2.0	37.0
82-83	29.667473072961265	35.0	32.0	35.0	2.0	37.0
84-85	29.49000675079038	35.0	32.0	35.0	2.0	36.0
86-87	29.346870685602546	35.0	31.5	35.0	2.0	36.0
88-89	29.223347931577138	35.0	31.0	35.0	2.0	36.0
90-91	29.1189264238115	35.0	31.0	35.0	2.0	35.5
92-93	29.05270035328394	35.0	31.0	35.0	2.0	35.0
94-95	28.980861992010453	35.0	31.0	35.0	2.0	35.0
96-97	28.914390471953773	35.0	31.0	35.0	2.0	35.0
98-99	28.855819753154268	35.0	31.0	35.0	2.0	35.0
100-101	28.804399376461653	35.0	31.0	35.0	2.0	35.0
102-103	28.145455767469805	34.0	29.5	35.0	2.0	35.0
104-105	28.677877013818694	35.0	31.0	35.0	2.0	35.0
106-107	28.732109477173935	35.0	31.0	35.0	2.0	35.0
108-109	28.800555733381657	35.0	31.0	35.0	2.0	35.0
110-111	28.908556125506774	35.0	32.0	35.0	2.0	35.0
112-113	28.872469103437112	35.0	32.0	35.0	2.0	35.0
114-115	28.815136400870696	35.0	31.5	35.0	2.0	35.0
116-117	28.75440305263463	35.0	31.0	35.0	2.0	35.0
118-119	28.68066199334724	35.0	31.0	35.0	2.0	35.0
120-121	28.586742457718444	35.0	31.0	35.0	2.0	35.0
122-123	28.48025820844866	35.0	31.0	35.0	2.0	35.0
124-125	27.959830197941493	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	96513.0
3	6281.0
4	4670.0
5	4379.0
6	4505.0
7	4911.0
8	5075.0
9	4936.0
10	5260.0
11	5455.0
12	5991.0
13	7490.0
14	9406.0
15	8164.0
16	5225.0
17	4482.0
18	3781.0
19	3314.0
20	3175.0
21	3233.0
22	3618.0
23	4529.0
24	6178.0
25	7932.0
26	8954.0
27	9976.0
28	12071.0
29	14188.0
30	17106.0
31	21494.0
32	27103.0
33	36940.0
34	56807.0
35	96010.0
36	209708.0
37	455076.0
38	162284.0
39	289.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.962863594696714	18.01655972157012	12.25672785267661	26.763848831056556
2	15.756597245172516	19.663217995572253	38.39313365153891	26.187051107716325
3	19.17462118708453	23.738497106220606	28.53066708057651	28.556214626118354
4	13.552263018680405	16.65229369587388	31.154584086335873	38.64085919910984
5	14.149966371249501	37.686520525441786	31.808036378570453	16.35547672473826
6	33.381530914686564	34.018155082776744	16.648004345212573	15.952309657324113
7	29.848935730829858	31.943006220555613	18.821823497862844	19.386234550751684
8	27.791109536552415	35.43358667388587	17.740448853208797	19.03485493635292
9	27.85216830074947	17.03951878421566	17.954981458278713	37.15333145675616
10-11	27.148051654838255	25.863958956565135	25.141740365220027	21.846249023376576
12-13	26.443722243255806	23.217707246950823	26.12497366673813	24.213596843055246
14-15	24.226276987379958	24.130770756081095	23.89334196800764	27.749610288531308
16-17	24.101769835923857	26.975126048173465	24.361478460225666	24.561625655677013
18-19	24.014952740754055	25.866035800726173	26.516198554929822	23.60281290358995
20-21	24.952859579846848	25.080894372039104	26.22812027249725	23.738125775616798
22-23	24.554718906446222	24.6421672636425	26.37052555905679	24.43258827085448
24-25	24.155649906536087	24.821334279978817	26.336920139412364	24.68609567407273
26-27	23.767052429653273	24.921482143825255	26.471193285748555	24.840272140772917
28-29	24.00407275406254	25.631577657483167	26.18497165633501	24.17937793211928
30-31	24.746585429432702	25.20774090629918	26.22073079348151	23.824942870786607
32-33	24.323379940275185	25.17610353885492	26.33680874023122	24.163707780638674
34-35	24.331215016015488	25.533212180535	26.347243130198166	23.788329673251347
36-37	24.38773153391474	25.412938197962287	26.371268220264405	23.828062047858573
38-39	24.374066567694683	25.517913359658195	26.317908012497504	23.790112060149614
40-41	24.380119256536716	25.266188343338218	26.423662968461407	23.93002943166366
42-43	24.260402269869715	25.51416292055976	25.9543382183112	24.271096591259322
44-45	23.674219778701815	25.716129635969754	26.44616560305204	24.16348498227639
46-47	23.776669892291846	25.61523911091571	25.88226294811249	24.725828048679958
48-49	23.967570955708428	25.763102957351197	25.609706284919003	24.659619802021375
50-51	23.87306731703984	25.94835979558993	25.835920888757517	24.34265199861271
52-53	24.11539766908353	25.886013387210927	25.675914531577583	24.322674412127952
54-55	23.73968536415278	25.472016897027792	26.169004440371364	24.61929329844806
56-57	23.94109508365707	25.7860511886664	25.6580906625949	24.61476306508163
58-59	23.89025992399605	25.944238025887685	25.864290546888284	24.301211503227975
60-61	24.315433465353742	25.727603751627353	25.976023925573465	23.980938857445437
62-63	24.25999380620553	25.75779292971677	25.95738312926241	24.02483013481529
64-65	24.114729273996684	25.294483735348223	25.924223306342547	24.666563684312546
66-67	23.92015203760242	25.58995149679653	25.953669823224352	24.536226642376693
68-69	23.65238553919803	25.534400438467173	26.111448196781456	24.701765825553338
70-71	23.756023910720238	25.497490176448878	26.113936111826952	24.63254980100393
72-73	23.736083457295866	26.04163804326596	25.504396925679668	24.71788157375851
74-75	23.632816416377462	26.010854736210455	25.648807397499755	24.707521449912328
76-77	23.696165417386737	25.403283602263333	25.818654015680547	25.081896964669376
78-79	23.558550295616293	25.440416662643916	26.22986552633514	24.77116751540465
80-81	23.859253818578264	25.600794350427662	25.68133595839315	24.858615872600925
82-83	23.974514837999596	25.711710801784466	25.79975328794683	24.514021072269106
84-85	23.901845438834794	25.983636202951484	25.400981352519736	24.713537005693983
86-87	23.476300566873302	26.138703863100805	25.609372087375576	24.775623482650317
88-89	23.397095749081515	25.72337058274397	25.748695329923528	25.13083833825099
90-91	23.803181119741673	25.502763817789287	25.84379435086487	24.85026071160417
92-93	23.701961192051257	25.861964608309307	25.745811834734806	24.690262364904633
94-95	23.64847577900606	25.72276797567039	25.542486766690864	25.086269478632683
96-97	23.379330243107425	25.731644960447557	25.599227926254926	25.28979687019009
98-99	23.249231902646027	25.759612449675416	25.49080622558037	25.50034942209818
100-101	23.241471093026487	25.922626584746183	25.58493853364515	25.250963788582176
102-103	23.020826448245053	25.79221527668957	25.71936021222287	25.467598062842505
104-105	23.383653581223744	25.844238694282772	25.505807981974126	25.26629974251936
106-107	23.375324682558396	25.62035488105339	25.63988689257208	25.364433543816133
108-109	23.301516335840446	25.60689638526479	25.692377102901776	25.399210175992987
110-111	23.13160599597179	26.06089547450286	25.61722802160261	25.19027050792274
112-113	23.156048620977245	26.182679946557624	25.49615780800106	25.165113624464077
114-115	23.138135612416278	26.161812067004504	25.447890522622803	25.25216179795641
116-117	22.932920213204447	25.96180767782571	25.56830629906699	25.53696580990285
118-119	22.96317283398663	25.80096189693919	25.666391386299058	25.569473882775124
120-121	22.90677069872589	25.79461094921085	25.827993595284987	25.470624756778275
122-123	22.81841039235731	26.02801581445678	25.505991691728752	25.64758210145716
124-125	22.61086551690206	25.85967772180994	25.497667112882773	26.031789648405223
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	46.0
1	46.5
2	81.5
3	101.0
4	87.5
5	100.0
6	195.5
7	515.0
8	747.5
9	880.0
10	927.5
11	806.0
12	780.0
13	860.0
14	947.0
15	1022.5
16	1108.5
17	1194.0
18	1286.0
19	1335.5
20	1437.0
21	1571.5
22	1693.5
23	1853.5
24	2058.5
25	2441.5
26	2962.0
27	3624.0
28	4344.0
29	5217.5
30	6486.5
31	7937.0
32	9776.0
33	11885.0
34	13900.5
35	15917.0
36	18375.0
37	21066.0
38	23419.0
39	25616.0
40	28032.0
41	30633.0
42	33284.0
43	36731.0
44	40468.0
45	44738.5
46	48538.0
47	51952.0
48	56659.5
49	60967.0
50	63783.0
51	65055.5
52	64398.0
53	62644.5
54	61113.5
55	58542.0
56	54380.0
57	49572.0
58	45029.0
59	40004.5
60	33888.5
61	28152.0
62	22838.5
63	17382.0
64	12660.5
65	9420.5
66	7096.5
67	5378.0
68	4158.5
69	3135.5
70	2417.5
71	1914.0
72	1422.5
73	1096.0
74	864.0
75	621.0
76	464.5
77	352.0
78	264.0
79	211.5
80	181.0
81	160.5
82	150.0
83	129.5
84	101.0
85	95.0
86	87.5
87	76.5
88	74.0
89	76.0
90	75.5
91	65.5
92	66.0
93	66.0
94	56.0
95	50.0
96	41.0
97	32.5
98	23.5
99	10.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.34897650145673
2	0.0
3	0.0
4	0.017229740016591052
5	0.1797982783627885
6	0.04901563970237109
7	0.02428502148890204
8	0.007426612076116832
9	0.006386886385460476
10-11	0.002450781985118555
12-13	0.05774190889180837
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	1.4853224152233666E-4
92-93	4.8272978494759417E-4
94-95	1.1139918114175249E-4
96-97	4.084636641864258E-4
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0
106-107	1.1139918114175249E-4
108-109	4.8272978494759417E-4
110-111	3.713306038058416E-4
112-113	8.911934491340199E-4
114-115	1.856653019029208E-4
116-117	5.941289660893466E-4
118-119	2.2279836228350497E-4
120-121	7.426612076116833E-5
122-123	0.0014481893548427822
124-125	1.1139918114175249E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1346509.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.08236624939043
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.36665290082226	55.751480562273336
2	8.53926322473701	11.285894398340437
3	2.7129521088106103	5.378348846144365
4	1.324181987138996	3.5002031621985896
5	0.7548445032678462	2.49409554631425
6	0.5081034218654904	2.0146005849770323
7	0.34901269374191707	1.614450925747779
8	0.22778359567090437	1.2041983195782213
9	0.1806962177031481	1.0746750274325119
>10	0.9762696394105547	11.380515250015497
>50	0.04494806430840997	1.9730860283403107
>100	0.014617025820716611	1.6338164728852436
>500	2.2487223405983427E-4	0.13128888923461957
>1k	4.4974446811966854E-4	0.563345986517762
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2576	0.1913095270807696	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2020	0.15001756393756	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1903	0.14132842780850333	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	7.426612076116833E-5
7	0.0	0.0	0.0	0.0	7.426612076116833E-5
8	0.0	0.0	0.0	0.0	7.426612076116833E-5
9	0.0	0.0	0.0	0.0	1.4853224152233666E-4
10-11	0.0	0.0	0.0	0.0	1.4853224152233666E-4
12-13	0.0	0.0	0.0	0.0	1.856653019029208E-4
14-15	0.0	0.0	0.0	0.0	4.4559672456700994E-4
16-17	0.0	0.0	0.0	0.0	5.198628453281783E-4
18-19	0.0	0.0	0.0	7.426612076116833E-5	5.198628453281783E-4
20-21	0.0	0.0	0.0	7.426612076116833E-5	5.569959057087625E-4
22-23	0.0	0.0	0.0	2.2279836228350497E-4	6.312620264699308E-4
24-25	0.0	0.0	0.0	3.713306038058416E-4	6.68395086850515E-4
26-27	0.0	0.0	0.0	5.198628453281783E-4	6.68395086850515E-4
28-29	0.0	0.0	0.0	0.0015595885359845347	6.68395086850515E-4
30-31	0.0	0.0	0.0	0.007129547593072159	7.05528147231099E-4
32-33	0.0	0.0	0.0	0.018603663250672666	7.426612076116832E-4
34-35	0.0	0.0	0.0	0.03200869804806355	8.169273283728515E-4
36-37	0.0	0.0	0.0	0.04838437767590116	8.169273283728515E-4
38-39	0.0	0.0	0.0	0.08106147081081522	8.169273283728515E-4
40-41	0.0	0.0	0.0	0.1315624329284097	8.169273283728515E-4
42-43	0.0	0.0	0.0	0.1782015567664234	8.540603887534357E-4
44-45	0.0	0.0	0.0	0.22562047487242937	8.911934491340199E-4
46-47	0.0	0.0	0.0	0.2726309293142489	9.654595698951882E-4
48-49	0.0	0.0	0.0	0.32517420975277556	0.0010397256906563566
50-51	0.0	0.0	0.0	0.3864437593807394	0.0010397256906563566
52-53	0.0	0.0	0.0	0.45376599785073846	0.001113991811417525
54-55	0.0	0.0	0.0	0.5255813366267882	0.0011882579321786932
56-57	0.0	0.0	0.0	0.6033379650637315	0.0012253909925592774
58-59	0.0	0.0	0.0	0.6953165556264385	0.0012625240529398616
60-61	0.0	0.0	0.0	0.7874065453702872	0.0012625240529398616
62-63	0.0	0.0	0.0	0.8989542587535619	0.0012625240529398616
64-65	0.0	0.0	0.0	0.9930865668183428	0.0012625240529398616
66-67	0.0	0.0	0.0	1.1022948973976407	0.00133679017370103
68-69	0.0	0.0	0.0	1.2304782218314174	0.0014110562944621983
70-71	0.0	0.0	0.0	1.3576589536349182	0.0014110562944621983
72-73	0.0	0.0	0.0	1.4737369003846243	0.0017081207775068714
74-75	0.0	0.0	0.0	1.6013260958523112	0.0017081207775068714
76-77	0.0	7.426612076116833E-5	0.0	1.7615181183341515	0.0017823868982680398
78-79	0.0	7.426612076116833E-5	0.0	1.9381229535042097	0.0017823868982680398
80-81	0.0	7.426612076116833E-5	0.0	2.1021396812052497	0.001856653019029208
82-83	0.0	7.426612076116833E-5	0.0	2.2696840496424455	0.0019309191397903764
84-85	0.0	7.426612076116833E-5	0.0	2.45494088788118	0.0019309191397903764
86-87	0.0	7.426612076116833E-5	0.0	2.6725406217114034	0.0019309191397903764
88-89	0.0	7.426612076116833E-5	0.0	2.908298422067732	0.0019309191397903764
90-91	0.0	7.426612076116833E-5	0.0	3.1560502009269897	0.0019309191397903764
92-93	0.0	7.426612076116833E-5	0.0	3.382933199852359	0.0019309191397903764
94-95	0.0	7.426612076116833E-5	0.0	3.615571823136719	0.002005185260551545
96-97	0.0	7.426612076116833E-5	0.0	3.887460091243356	0.002042318320932129
98-99	0.0	7.426612076116833E-5	0.0	4.2041679632293585	0.002079451381312713
100-101	0.0	7.426612076116833E-5	0.0	4.533204011261715	0.002079451381312713
102-103	0.0	7.426612076116833E-5	0.0	4.8842599640997575	0.002079451381312713
104-105	0.0	7.426612076116833E-5	0.0	5.243448057161148	0.0021165844416932973
106-107	0.0	7.426612076116833E-5	0.0	5.637801158402952	0.0021537175020738815
108-109	0.0	7.426612076116833E-5	0.0	6.07058697713866	0.0021537175020738815
110-111	0.0	7.426612076116833E-5	0.0	6.513101657694081	0.0021537175020738815
112-113	0.0	7.426612076116833E-5	0.0	6.942508367935157	0.0021537175020738815
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2120	0.0	70.45895	1
GTATCAA	3460	0.0	47.83389	1
GTACATG	6985	0.0	38.834835	1
TACATGG	7195	0.0	37.438934	2
ACATGGG	7315	0.0	36.90623	3
CATGGGG	6530	0.0	34.77184	4
TCAACGC	4650	0.0	33.83505	4
ATGGGGG	4020	0.0	33.652367	5
TATCAAC	4845	0.0	33.211296	2
ATCAACG	4945	0.0	32.17813	3
CAACGCA	4860	0.0	32.005165	5
AACGCAG	4960	0.0	31.237423	6
TGGGGGG	3290	0.0	28.618359	6
GTGGTAT	950	0.0	27.67331	1
ACGCAGA	5985	0.0	25.780416	7
TGGTATC	1020	0.0	24.53946	2
CGCAGAG	6440	0.0	24.047901	8
AGAGTAC	6360	0.0	23.085186	10-11
GAGTACT	3460	0.0	22.623348	12-13
CAGAGTA	6745	0.0	22.077393	9
>>END_MODULE