FastQCFastQC Report
Mon 15 Aug 2016
SRR2921282_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921282_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779845
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7550.059.902661
GTATCAA12350.048.6669241
TCAACGC13150.045.250764
CAACGCA14050.042.7756545
GTACATG39500.042.183281
TACATGG40700.040.0597042
AACGCAG15300.039.6672866
ATCAACG15550.038.6493843
ACATGGG41500.038.1403923
TATCAAC17250.034.4955062
GTATCGC700.002788224734.000536
CATGGGG35200.033.640474
ATGGGGG20000.031.8350375
AGTACAT25750.030.5034052
GAGTACA26850.030.3637771
ACGCAGA20200.029.4521277
CTATGCG1254.884372E-528.5567849
CGCAGAG21050.028.262858
GTATTAG2353.4015102E-1027.8550721
TAGAGCG1150.001004054725.871635
Produced by FastQC (version 0.11.5)